scholarly journals Variations in the Levels of Individual Phenolic Compounds in Grapevine Latent Buds during Eco-Dormancy, Following Chemically-Induced Stress Conditions

Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1798
Author(s):  
Ioannis Daskalakis ◽  
Maritina Stavrakaki ◽  
Kyriaki Sotirakoglou ◽  
Katerina Biniari
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Content ◽  
Growth Cycle ◽  
Stress Conditions ◽  
Economic Activities ◽  
Induced Stress ◽  
Chemically Induced ◽  
Chilling Temperatures ◽  
Positive Correlations ◽  
The Individual

The goal of viticulture in Greece, where it is one of the country’s briskest economic activities, is satisfying grape yields. Nevertheless, owing to the ongoing climate change, that goal is impeded by a major obstacle in the form of an insufficient period of chilling temperatures which are becoming briefer. Consequently, the shorter period of cold leads to poor budbreak which, in turn, results in reduced yields. In order to meet the chilling requirements that several plants (including the grapevine) require for bud dormancy release, agronomists have resorted to treatments with chemical regulators. This study aimed at identifying and quantifying during eco-dormancy the individual phenolic compounds, together with their possible variations, in the latent buds of the ‘Prime©’ and ‘Ralli’ table grape cultivars. The study induced chemical stress by means of four different solutions, at three discrete dates per year, for three consecutive years. Phenolics were analyzed via HPLC. Their quantitative analysis showed variations both between the varieties and between the samples of those varieties. The analysis indicated that the phenolic content continued progressing during the annual growth cycle. Higher rates of increase in the phenolic content were observed in the first three days following each application, which implies an immediate stress response mechanism. Strong positive correlations were detected between several polyphenols. Luteolin, despite registering a positive correlation with quercetin, resveratrol, o-coumaric, did not do so with the remaining polyphenols. The results confirmed that chemically-induced stress conditions affect the phenolics content. Moreover, depending on the date of application, significant changes appear in the variations of those phenolics.

scholarly journals Variations in phenolic levels in grapevine buds at eco-dormancy after chemically-induced stress conditions

2021 ◽  
Author(s):  
Ioannis Daskalakis ◽  
Maritina Stavrakaki ◽  
Kyriaki Sotirakoglou ◽  
Katerina Biniari
Keyword(s):  
Growth Cycle ◽  
Table Grape ◽  
Stress Conditions ◽  
Economic Activities ◽  
Induced Stress ◽  
Chemically Induced ◽  
Chilling Temperatures ◽  
Bud Dormancy Release ◽  
Positive Correlations ◽  
The Individual

Viticulture is one of the briskest economic activities worldwide. A major obstacle impeding such grape yields to satisfy the demand for increased production is the insufficient period of chilling temperatures which, due to climate change, is becoming briefer. The shorter period of cold leads to poor budbreak which, in turn, results in reduced yields. To combat this issue, agronomists have resorted to treatments with chemical regulators to meet the chilling requirement for bud dormancy release several fruit-bearing plants have, including grapevines. This study aimed at identifying and quantifying during eco-dormancy the individual polyphenolic compounds, and their possible variations, in the latent buds of the 'Prime©' and 'Ralli' table grape cultivars. The study induced chemical stress by means of four different solutions, at three discrete dates per year, for three consecutive years. Phenolics in the latent buds of the studied varieties were analyzed via HPLC. Their quantitative analysis showed variations both between the varieties and between the samples of those varieties collected after the eight treatments. The analysis indicated that the concentration of phenolics continued progressing during the annual growth cycle of the grapevine, with strong positive correlations being detected between catechin, epicatechin, chlorogenic acid, o-coumaric, piceid, procyanidins B1-B2, rutin, ε-viniferin. Luteolin registered a positive correlation with quercetin, resveratrol, o-coumaric, but not with the remaining polyphenols. The results confirmed that (a) the concentration of phenolics of the latent buds is affected by chemically-induced stress conditions, (b) depending on the date of application, significant changes appear in the variations of those phenolics.

scholarly journals Development of a rapid resolution HPLC method for the separation and determination of 17 phenolic compounds in crude plant extracts

2011 ◽  
Vol 9 (1) ◽  
pp. 133-142 ◽  
Author(s):  
Aleksandra Mišan ◽  
Neda Mimica-Dukić ◽  
Anamarija Mandić ◽  
Marijana Sakač ◽  
Ivan Milovanović ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Hplc Method ◽  
Total Phenolic ◽  
Rapid Separation ◽  
Rapid Resolution ◽  
Peak Areas ◽  
Hydroxybenzoic Acids ◽  
The Individual

AbstractRapid Resolution HPLC/DAD method, on a 1.8 µm, 4.6×50 mm column, was developed to enable a rapid separation of a mixture of 17 compounds, which consisted of hydroxybenzoic acids, hydroxycinnamic acids, flavones, flavonols, flavanone, flavonol-glycoside and antraquinone, in a single run, within 22 minutes. The developed method is precise, accurate and sensitive enough for simultaneous quantitative evaluation of major compounds in crude and hydrolyzed extracts of parsley, buckthorn, mint, caraway and birch. In order to overcome the inability to quantify all the phenolic compounds present in the samples caused by lack of external standards, HPLC approaches for the total phenolic content estimation based on sum of all integrated peak areas were made. These results were compared with the total phenolic content determined by Folin-Cioacalteu method. Although the correlation between the series of data was not significant (pHPLC was not high in the case of parsley, buckthorn and mint extract. Regarding the obtained results, the HPLC approach could serve as an excellent tool for total phenolic content estimation, without the need for complete identification of the individual compounds.

scholarly journals Correlations Between Phenolic Composition and Perceived Astringency of Wines

2020 ◽  
Vol 10 (22) ◽  
pp. 8020
Author(s):  
Kyeong-Ok Choi ◽  
Dong Hoon Lee ◽  
Seo Jun Park ◽  
Dongjun Im ◽  
Youn Young Hur
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Tannin Content ◽  
Total Anthocyanin ◽  
Breeding Lines ◽  
Total Anthocyanin Content ◽  
Positive Correlations ◽  
Grape Breeding

The astringent sensation of wines is attributable to the composition and concentration of phenolic compounds. However, in many cases, experimental results have shown a gap in relation to sensory astringency. The aim of the present study was to evaluate the relationships between the experimental evidence of astringent attributes and perceived astringency. To this end, the concentrations of phenolic compounds, namely, the total phenolic content (TPC), the polymeric tannin content (PTC), the proanthocyanidin content (PAC) and the total anthocyanin content (TAC), in wines made from a variety of grape breeding lines were estimated and their relationships to perceived astringency were evaluated. The TPC and the PTC showed strong positive correlations with perceived astringency. In contrast, the PAC showed a moderate correlation with perceived astringency, which was lower than that shown by the PTC despite their similar phenolic compositions. Unlike the other attributes, the TAC showed a weak correlation with perceived astringency. We concluded that the TPC and the PTC showed good correlations with perceived astringency and could be used as an index for predicting the astringency of wines.

scholarly journals Improvement of Phenylpropanoid Production with Elicitor Treatments in Pimpinella brachycarpa Nakai

Horticulturae ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 108
Author(s):  
Nam Su Kim ◽  
Dae Hui Jung ◽  
Chung Ryul Jung ◽  
Kwon Seok Jeon ◽  
Hong Woo Park ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Time Course ◽  
Phenolic Content ◽  
Plant Secondary Metabolites ◽  
Fold Increase ◽  
Treatment Type ◽  
Elicitor Treatment ◽  
Therapeutic Properties ◽  
Elicitor Treatments ◽  
The Individual

Pimpinella brachycarpa Nakai, known as cham-na-mul in Korea, is a popular edible herb and vegetable. Phenolic compounds are recognized as a vital group of plant secondary metabolites that provide innumerable, valuable therapeutic properties. Elicitors are biofactors or chemicals from diverse sources that can trigger morphological and physiological responses in the target organism. This study examined the effect of methyl jasmonate (MeJA), salicylic acid (SA), and chitosan treatment on the accretion of phenolic compounds in P. brachycarpa Nakai. This plant was harvested under different concentration of elicitor treatment for time course. Eight phenolic compounds including were detected in response to elicitor using HPLC. While the untreated controls showed the lowest phenolic content, treatment with 0.3% chitosan, 0.1 mM SA, and 0.1 mM MeJA resulted in 1.43-, 1.39-, and 1.35-fold increase in the phenolic content, respectively. The highest content of most of the individual phenolic compounds followed a similar trend according to treatment type, with chitosan treatment showing the highest content, followed by SA and then MeJA treatments. Thus, we demonstrate that the treatment with optimal concentrations of these elicitors for an optimal period of time increases the production of phenolic compounds in P. brachycarpa Nakai.

scholarly journals Post-Anthesis Mobilization of Stem Assimilates in Wheat under Induced Stress

2021 ◽  
Vol 13 (11) ◽  
pp. 5940
Author(s):  
Mian Faisal Nazir ◽  
Zareen Sarfraz ◽  
Naimatullah Mangi ◽  
Muhammad Kausar Nawaz Shah ◽  
Talat Mahmood ◽  
...  
Keyword(s):  
Phenotypic Variation ◽  
Dry Matter ◽  
Genetic Basis ◽  
Grain Filling ◽  
Stress Conditions ◽  
Induced Stress ◽  
Chemically Induced ◽  
Direct Cross ◽  
Stem Reserves ◽  
Source Sink

Stem reserves in grain crops are considered important in grain filling under post-anthesis stress in the absence/low availability of photosynthetic assimilates. Considerable variation is present among genotypes for stem reserve translocation in wheat. Therefore, this study aimed to exploit the phenotypic variation for stem reserve translocation in wheat under control and chemically induced stress conditions. The phenotypic variation among six parents and their corresponding direct cross combinations was evaluated under induced stress conditions. The results signify the presence of considerable variation between treatments, genotypes, and treatment-genotype interactions. The parent LLR-20 depicted the highest translocation of dry matter and contribution of post-anthesis assimilates under induced-stress conditions. Similarly, cross combinations Nacozari × LLR22, Nacozari × LLR 20, Nacozari × Parula, Nacozari × LLR 21, LLR 22 × LLR 21, and LLR 20 × LLR 21 showed higher source-sink accumulation under induced-stress conditions. The selected parents and cross combinations can be further utilized in the breeding program to strengthen the genetic basis for stress tolerance in wheat.

scholarly journals Phenolic Response to Walnut Anthracnose (Ophiognomonia leptostyla) Infection in Different Parts of Juglans regia Husks, Using HPLC-MS/MS

Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 659
Author(s):  
Aljaz Medic ◽  
Anita Solar ◽  
Metka Hudina ◽  
Robert Veberic
Keyword(s):  
Phenolic Compounds ◽  
Bacterial Infections ◽  
High Performance ◽  
Phenolic Content ◽  
Juglans Regia ◽  
Similar Response ◽  
The Individual ◽  
Walnut Husk ◽  
Degrees Of Severity ◽  
Made In

This study compares the individual phenolic response of husk tissues of Juglans regia L., infected to different degrees of severity with walnut anthracnose, which is one of the most serious and widespread walnut diseases worldwide. A comparison among three differently susceptible cultivars, ‘Franquette’, ‘Milotai 10’ (‘M10’), and ‘Milotai intenziv’ (‘M10-37’), is made. In our methodology, high performance liquid chromatography coupled with mass spectrometry is used to identify and quantify the compounds. Our results show that flavanols, flavonols, and naphthoquinones account for more than 95% of the phenolic compounds identified in the walnut husk. The higher total analyzed phenolic content in tissues is more affected by walnut anthracnose confirmed that phenolics play a major role in the plant’s response against pathogens. A difference between cultivars is observed, since French cultivar ‘Franquette’ responds differently to walnut anthracnose infection than Hungarian cultivars ‘M10’ and ‘M10-37’. Naphthoquinones and flavanols have a very similar response to walnut anthracnose infection. The resistance of cultivars may be due to the reaction time of the plant and the speed with which it recognizes the pathogen and responds quickly to the infection by containing it while it has not yet spread. Flavonols may be the most important phenolic compounds in disease control, since they respond more rapidly to infection than flavanols and naphthoquinones. They also play an inhibitory role in the early stages of viral and bacterial infections.

scholarly journals Characterization of Phenolic Compounds and Their Contribution to Sensory Properties of Olive Oil

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2041 ◽  
Author(s):  
Vasilisa Pedan ◽  
Martin Popp ◽  
Sascha Rohn ◽  
Matthias Nyfeler ◽  
Annette Bongartz
Keyword(s):  
Phenolic Compounds ◽  
Olive Oil ◽  
Phenolic Content ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Sensory Properties ◽  
Total Phenolic ◽  
Sensory Data ◽  
Positive Correlations

Olive oil is not only known for its pungent, bitter, and fruity taste, but also for its health potential, which is often hypothesized to depend on its phenolic compounds. One hundred extra virgin olive oil samples (monocultivaric as well as blends of varieties) were assessed with regard to their sensory properties and phenolic compound composition. Nineteen phenolic compounds have been determined and correlated with sensory data. In all olive oil samples, oleocanthal and oleacein were the most abundant phenolic compounds, with average amounts of 77.9 mg/kg and 41.8 mg/kg, respectively. The highest correlation coefficient between a sensory descriptor and the phenolic compounds was found for the bitter taste sensation and the total phenolic content with r = 0.72 and in particular, for 3,4-DHPEA-EA, with r = 0.57. Intensity plots were assessed for the three main sensory descriptors fruitiness, bitterness, pungency, and for the quality factor harmony, which is associated with the degree of ripeness aroma of olive oil. Positive correlations for the aroma descriptors freshly cut grass, leaves, and nuts, and the phenolic compounds were especially observed for oleoside 11-methylester and vanillic acid. The present study provides a comprehensive database of phenolic compounds in olive oils from six different varieties and seven countries.

Survey of Chinese Cowpea Cultivars for Phenolic Compounds and Antioxidant Activity

2018 ◽  
Vol 17 (4) ◽  
pp. 337-348
Author(s):  
Bai Zhouya ◽  
Huang Xiaojun ◽  
Meng Jinxia ◽  
Kan Lijiao ◽  
Nie Shaoping
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Jiangxi Province ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Comprehensive Investigation ◽  
Cowpea Cultivars ◽  
P Distribution

Distribution of phenolic compounds and antioxidant activities was surveyed in 24 varieties of Chinese cowpea from 4 provinces. Identity of phytochemicals were determined by UPLC-ESIQTOF-MS/MS and quantified by HPLC-ESI-QqQ-MS/MS. Seven phenolic acids, 16 flavonoids and 9 other compounds were identified and several of these were quantified. Quercetin-3-glucoside and kaempferol-glucoside were the major phenolic compounds. In addition, our study indicated that OJYDH cultivar from Jiangxi province had the highest total phenolic content, total flavonoid content and stronger antioxidant activities than other cowpea cultivars. This study made a comprehensive investigation on antioxidants from cowpea and provided the useful data to support its function.

Microwave Pretreatment of Vegetable Materials to Increase the Extraction Yield of Natural Products

2018 ◽  
Vol 69 (8) ◽  
pp. 1976-1979
Author(s):  
Ioana Asofiei ◽  
Ioan Calinescu ◽  
Adina Ionuta Gavrila ◽  
Daniel Ighigeanu ◽  
Diana Martin
Keyword(s):  
Natural Products ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Microwave Pretreatment ◽  
Pretreatment Time ◽  
Conventional Extraction

It was designed and built a laboratory experimental installation (LEI) for the microwave pretreatment of vegetable materials. To study the influence of microwave pretreatment on the total phenolic content (TPC), a conventional extraction of polyphenols from treated and untreated fresh sea buckthorn leaves was performed. For short extraction times, the amount of phenolic compounds was higher for the extracts obtained from treated leaves, but a long pretreatment time (28 s) led to a decrease in TPC. The qualitative analysis showed that the chemical composition is not affected by the microwave pretreatment.

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