scholarly journals Association between THRSP Gene Polymorphism and Fatty Acid Composition in Milk of Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1144
Author(s):  
Daniel Polasik ◽  
Jacek Golińczak ◽  
Witold Proskura ◽  
Arkadiusz Terman ◽  
Andrzej Dybus
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
De Novo ◽  
Potential Marker ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Lactating Mammary Gland ◽  
A Chain

Thyroid hormone-inducible hepatic protein is involved in the de novo synthesis of fatty acids in the lactating mammary gland. Different variants of the gene that encodes this protein may be associated with its different activity. The primary aim of this study was to find polymorphism in the THRSP gene and estimate the relationship between individual genotypes and fatty acid composition in milk. Investigations were carried out on 224 cows represented by two breeds—Jersey (n = 80) and Polish Holstein-Friesian (n = 144). Polymorphism in THRSP was detected by Sanger sequencing; however, genotypes were determined by the PCR-RFLP method. It was shown that the analyzed variant had a significant (p < 0.05) influence on palmitic and stearic fatty acids as well as on fatty acids with a chain length of 14, 16, and 6–16 in Jersey breed and on caproic, palmitic, myristoleic, and palmitoleic fatty acids in H-F. Obtained results indicated that analyzed SNP in bovine THRSP gene (rs42714482) may be considered as a potential marker for fatty acid composition in milk

The composition and metabolism of fatty acids in Ips paraconfusus Lanier (Coleoptera: Scolytidae)

1972 ◽  
Vol 50 (10) ◽  
pp. 1263-1267 ◽  
Author(s):  
K. R. Penner ◽  
J. S. Barlow
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Sexual Dimorphism ◽  
Acid Composition ◽  
De Novo ◽  
Monounsaturated Fatty Acids ◽  
Ips Paraconfusus ◽  
Chain Elongation ◽  
De Novo Synthesis

The fatty acid composition of newly emerged Ips paraconfusus Lanier shows no sexual dimorphism and is approximately as follows: C14:0, 0.5%; C16:0, 23.0%; C16:1, 6%; C18:0, 3%; C18:1, 55%; C18:2, 9%; C18:3, 2%. Both sexes, but particularly the female, use up fatty acids, particularly the monounsaturated acids, during reproduction. Isotope from 1-14C-acetate injected into newly emerged females appeared in all saturated and monounsaturated fatty acids within 30 min. There was evidence of de novo synthesis of C14:0 and C16:0, chain elongation of C16:0 to C18:0, and desaturation of C16:0 and C18:0 to yield C16:1 and C18:1 respectively.

scholarly journals Influence of Diet on Fatty-Acid Composition of Depot Fat in Western Sandpipers (Calidris Mauri)

The Auk ◽  
2003 ◽  
Vol 120 (2) ◽  
pp. 337-345 ◽  
Author(s):  
Oliver Egeler ◽  
Dana Seaman ◽  
Tony D. Williams
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
De Novo ◽  
Calidris Mauri ◽  
Total Percentage ◽  
Western Sandpipers ◽  
Experimental Treatments

Abstract Western Sandpipers (Calidris mauri) have been previously shown to undergo seasonal changes in the fatty acid composition of their fat stores, even though they do not show the marked seasonal variation in diet common to many migratory passerines. We investigated the effect of dietary fatty acid composition on the fatty acid composition of adipose tissue in captive Western Sandpipers by feeding birds experimental diets with different fatty acid composition. In addition, we determined the effect of total percentage of fat content of the diet (5 vs. 10%) on fatty acid composition of depot fat. Birds maintained normal body mass (24–27 g) throughout all experimental treatments. Most adipose fatty acids were sensitive to dietary manipulation to some extent. Changes in fatty acid composition of the diet had the largest effect on adipose tissue composition for the essential polyunsaturated fatty acid linoleate (18:2), whereas it had the least effect for the monounsaturated fatty acid oleate (18:1). The saturated fatty acid palmitate (16:0) demonstrated an intermediate capacity to alter fatty acid composition of adipose tissue. Total amount of fat in the diet did not influence the effect of diet on fatty acid deposition. Results of dietary manipulations in this study suggest that diet does explain some of the variation in fatty acid composition observed during migration in Western Sandpipers, but that certain fatty acids can be modulated independently of diet (probably through de novo synthesis, postabsorption modification, or both).

Regulation Of The Fatty Acid Composition Of Platelet Phospholipids

1981 ◽  
Author(s):  
M L McKean ◽  
J B Smith ◽  
M J Silver
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Unsaturated Fatty Acids ◽  
De Novo ◽  
Membrane Phospholipids ◽  
De Novo Biosynthesis ◽  
Coa Transferase

The fatty acid composition of cell membrane phospholipids does not remain constant after de novo biosynthesis, but undergoes continual remodelling. One of the major routes for remodelling probably includes the deacylation-reacylation steps of the Lands Pathway. This has been shown to be important for the incorporation of long chain, polyunsaturated fatty acids into phospholipids by liver and brain. An understanding of the mechanisms involved in these processes in platelets is especially important in light of the large stores of arachidonic acid (AA) in platelet phospholipids and the role of AA in hemostasis and thrombosis. Previous results from this laboratory have shown that the turnover of radioactive AA, 8,11,14-eicosatrienoic and 5,8,11,14,17-eicosapentaenoic acids in the phospholipids of resting platelets is more rapid than the turnover of radioactive C16 and C18 saturated and unsaturated fatty acids. However, little is known about how fatty acids, especially AA and its homologues, are incorporated into platelet phospholipids during de novo biosynthesis or how they are exchanged during remodelling.At least three enzymes are involved in the deacylation- reacylation of phospholipids: phospholipase A2; acyl CoA synthetase; and acyl CoA transferase. We have studied acyl CoA transferase and have found considerable activity in human platelet membranes. Experiments are in progress to determine the substrate specificity and other properties of this enzyme.

Intraerythrocytic Plasmodium falciparum utilize a broad range of serum-derived fatty acids with limited modification for their growth

Parasitology ◽  
2006 ◽  
Vol 133 (4) ◽  
pp. 399-410 ◽  
Author(s):  
F. MI-ICHI ◽  
K. KITA ◽  
T. MITAMURA
Keyword(s):  
Fatty Acids ◽  
Plasmodium Falciparum ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
De Novo ◽  
Severe Form ◽  
Lipid Species ◽  
New Strategy

Plasmodium falciparum causes the most severe form of malaria. Utilization of fatty acids in serum is thought to be necessary for survival of this parasite in erythrocytes, and thus characterization of the parasite fatty acid metabolism is important in developing a new strategy for controlling malaria. Here, we examined which combinations of fatty acids present in human serum support the continuous culture of P. falciparum in serum-free medium. Metabolic labelling and gas chromatography analyses revealed that, despite the need for particular fatty acids for the growth of intraerythrocytic P. falciparum, it can metabolize a broad range of serum-derived fatty acids into the major lipid species of their membranes and lipid bodies. In addition, these analyses showed that the parasite's overall fatty acid composition reflects that of the medium, although the parasite has a limited capacity to desaturate and elongate serum-derived fatty acids. These results indicate that the Plasmodium parasite is distinct from most cells, which maintain their fatty acid composition by coordinating de novo biosynthesis, scavenging, and modification (desaturation and elongation).

scholarly journals Acute-on-chronic effects of fatty acids on intestinal triacylglycerol-rich lipoprotein metabolism

2002 ◽  
Vol 88 (6) ◽  
pp. 661-669 ◽  
Author(s):  
Irene L. Black ◽  
Helen M. Roche ◽  
Anne-Marie Tully ◽  
Michael J. Gibney
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Dietary Fat ◽  
De Novo ◽  
Cell Model ◽  
Lipoprotein Metabolism ◽  
Chronic Effects ◽  
Fat Composition

Postprandial triacylglycerol (TAG) metabolism is an important metabolic state that has been associated with cardiovascular disease. The magnitude of the postprandial TAG response is determined by dietary fat composition, which alters intestinal and hepatic TAG-rich lipoprotein (TRL) metabolism. Caco-2 cell monolayers are morphologically and physiologically similar to the human intestinal enterocytes, hence they are a good model to study intestinal lipoprotein metabolism. To date only the acute effect of fatty acid composition on intestinal TRL metabolism in Caco-2 cells has been investigated. Little is known of the effect of habitual, or chronic, dietary fat composition on intestinal TRL metabolism. Using the Caco-2 cell model, the present study investigated the acute-on-chronic effect of fatty acid composition on TRL metabolism. Caco-2 cells were grown in the presence of 0·05 mM-PALMITIC ACID (PA; 16 : 0), -OLEIC ACID (OA; 18 : 1N-9),-EICOSAPENTAENOIC ACID (EPA; 20 : 5N-3) OR NO FATTY ACID (CONTROL) FOR 19 D, THEN ONE OF FOUR ACUTE TREATMENTS (CONTROL (BOVINE SERUM ALBUMIN (BSA; 5 G/L)) OR BSA (5 G/L) PLUS 0·5 Mm-PA, -OA or -EPA) were administered for 22 h. Significant acute×chronic interactions for the effect of fatty acid composition on cellular TAG:secretedde novoTAG, and cellularde novoTAG:de novophospholipid were observed. Thus the effect of a fatty acid was determined by the duration of exposure to the fatty acid intervention. Acute PA treatment increasedde novoTAG synthesis, but chronic PA supplementation did not. Acute and chronic OA treatments increasedde novoTAG secretion. For EPA, chronic supplementation had the greatest effect on TAG synthesis and secretion. The acute-on-chronic effects of fatty acids on apolipoprotein B metabolism were relatively minor compared with the changes noted for TRL lipid composition. The present study shows that the Caco-2 cell model is valuable for studying intestinal TRL metabolism and that fatty acids modulate this process, the nature of which can be determined by the length of exposure of the cell to the fatty acid.

Synthesis of fatty acids by the parasite Exeristes comstockii (Hymenop.) and two hosts, Galleria mellonella (Lep.) and Lucilia sericata (Dip.)

1972 ◽  
Vol 50 (8) ◽  
pp. 1105-1110 ◽  
Author(s):  
S. N. Thompson ◽  
J. S. Barlow
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Galleria Mellonella ◽  
De Novo ◽  
Lucilia Sericata ◽  
Fatty Acid Patterns ◽  
Direct Incorporation ◽  
Characteristic Fatty Acid

In contrast with many other insects, whose characteristic fatty acid compositions are influenced to a limited extent by dietary fatty acid composition, the fatty acid composition of the total lipid of the ichneumonid parasite Exeristes comstockii is very similar to that of its diet, that is, the host on which it is reared. When the parasite was reared on two hosts, Lucilia sericata and Galleria mellonella, with very different fatty acid patterns, it retained no characteristic fatty acid composition of its own.Radioisotope studies demonstrated that Exeristes comstockii, Galleria mellonella and Lucilia sericata incorporate 14C from 14C-1-acetate into fatty acids with radioactivity distributions consistent with de novo, elongation, and desaturation synthetic mechanisms.Exeristes comstockii was found to incorporate 14C-1-acetate into fatty acids at higher rates than either host. It appears, therefore, that the parasite has a fatty acid metabolism of its own, with respect to synthesis and turnover, although the fatty acids are qualitatively and quantitatively very similar to those in the host. The origin of the fatty acids of E. comstockii is partially explained by synthesis of fatty acids at different rates than its host, and direct incorporation of host fat, but the parasite appears to lack to great extent the control over pool size normally present in other insects.

scholarly journals Preliminary study of solute carrier family 23 member 3 (SLC23A3) gene as candidate marker for fatty acid traits in Kampung-Broiler crossbred chickens

2018 ◽  
Vol 43 (3) ◽  
pp. 201
Author(s):  
A. Gunawan ◽  
S. Y. Basril ◽  
K. Listyarini ◽  
A. Furqon ◽  
W. Bilyaro ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Coding Region ◽  
Candidate Marker ◽  
Pcr Rflp ◽  
Preliminary Study ◽  
Selection Of

SLC23A3 is one of the key genes which control the properties of the of fatty acids content in the meat. The aim of this study was to identify the association of SNP and mRNA expression of SLC23A3 as a candidate marker for fatty acid traits (FAs). Sixty two F2 Kampung × Broilers crossbred chickens population were used in this study. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken. The PCR-RFLP and qRT-PCR were used for association study and expression analysis of SLC23A3 gene with divergent fatty acid composition. A SNP in coding region c.22385690 A>C of the SLC23A3 gene was associated (P<0.05) with fatty acid composition including stearic acid (C18:0), elaidic acid (C18:1n9t), and linoleic acid (C18:2n6c). The SLC23A3 was detected in liver from high fatty acids (HFA) and low fatty acid (LFA) composition. However, gene expression of SLC23A3 were not differentially expressed between HFA and LFA. These results will explain better understanding of the key important role of the SLC23A3 in fatty acid traits within the liver and will propose SLC23A3 as a potential genomic selection for selection of chickens with fatty acid composition.

scholarly journals Der Einfluss der Fütterung auf die Zusammensetzung des intra-muskulären Fettes des <i>Musculus longissimus</i> und verschiedener Fettdepots von Jungbullen der Rassen Ungarisches Grauvieh und Holstein Friesian – 1. Mitteilung: Fettsäurezusammensetzung

2005 ◽  
Vol 48 (6) ◽  
pp. 537-546
Author(s):  
G. Holló ◽  
K. Nuernberg ◽  
I. Repa ◽  
I. Holló ◽  
J. Seregi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Acid Composition ◽  
Intramuscular Fat ◽  
Longissimus Muscle ◽  
Fat Deposits ◽  
Holstein Friesian

Abstract. Title of the paper: Effect of feeding on the composition of the intramuscular fat in longissimus muscle and different fatty tissues of Hungarian Grey and Holstein Friesian bulls. 1. Fatty acid profile The aim of this study was to analyse the effect of nutrition, genotype and nutrition x genotype interaction on the fatty acid composition of intramuscular fat from longissimus dorsi muscle and different fat deposits using Hungarian Grey cattle bulls (HG) in comparison to Holstein Friesian bulls (HF) kept under extensive and intensive conditions. The extensive group was kept on pasture and was fed a concentrate supplemented with linseed meal in the last month of the trial. The extensive diet influenced the linoleic and linolenic acid ratio and the CLA content of longissimus muscle more advantageous concerning human nutrition. The meat from HG contained more CLA and less n-6 fatty acids also in case of the intensive diet than that of HF bulls. In the fat deposits from HF the PUFA mainly the linoleic acid was in a significant higher proportion available, however in the fat deposits of HG was the percentage of linol- and linolenic acid favourable. The extensive nutrition resulted in a lower linoleic acid and a higher linolenic acid content in both breeds and all three fat deposits. The PUFA content in the fat samples was altered contrary in both analysed breeds because of the different diet. In case of the extensive UG was the PUFA higher in all three fat deposits. In conclusion, the fatty acid composition of beef can be modified favourably by extensive feeding and concentrate which is rich in unsaturated fatty acids, however the beef of Hungarian Grey has a more wholesome influence on human health, than that of Holstein-Friesian bulls.

scholarly journals The effect of polymorphism in the <i>FADS2</i> gene on the fatty acid composition of bovine milk

2019 ◽  
Vol 62 (2) ◽  
pp. 547-555 ◽  
Author(s):  
Witold Stanisław Proskura ◽  
Michał Liput ◽  
Daniel Zaborski ◽  
Zbigniew Sobek ◽  
Yu-Hsiang Yu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linolenic Acid ◽  
Acid Composition ◽  
Bovine Milk ◽  
Stearidonic Acid ◽  
Milk Fatty Acids ◽  
Direct Dna Sequencing ◽  
Holstein Friesian

Abstract. Polyunsaturated fatty acids (PUFAs) play a role in a wide variety of physiological processes. They are produced by a series of desaturation and elongation reactions. Δ-6-desaturase is a membrane-bound enzyme that catalyzes the conversion of α-linolenic acid (C18:3n-3) and linoleic acid (C18:2n-6) to stearidonic acid (18:4n-3) and γ-linolenic acid (18:3n-6). It is encoded by the FADS2 gene located on bovine chromosome 29. The aim of this study was to identify a single nucleotide polymorphism in the FADS2 gene and to determine possible associations with milk fatty acid composition in two breeds of dairy cattle, i.e., Jersey and Polish Holstein-Friesian. Direct DNA sequencing revealed the presence of an A-to-G substitution in intron 3 of the FADS2 gene (rs209202414). Both populations were genotyped with an appropriate PCR-RFLP assay. The following genotype distributions were observed: for Jerseys, AA = 0.24, AG = 0.63, and GG = 0.13; for Polish Holstein-Friesians, AA = 0.17, AG = 0.40, and GG = 0.43. In Jerseys, statistically significant relationships were found between the FASD2 genotypes and the following milk fatty acids: lauric (P=0.0486), behenic (P=0.0199), lignoceric (P=0.0209), oleic (P=0.0386), eicosatrienoic (P=0.0113), and docosadienoic (P=0.0181). In Polish Holstein-Friesian cows, significant associations were observed for erucic (P=0.0460) and docosahexaenoic (P=0.0469) acids. The study indicated the A-to-G substitution (rs209202414) in the bovine FADS2 gene as a potential genetic marker for fatty acid composition in cattle milk.

scholarly journals EFFECT OF TREATMENT WITH PREPARATIONS CONTAINING HUMIC ACIDS ON THE OIL CONTENT AND FATTY ACID COMPOSITION OF BRASSICA NAPUS L.

2021 ◽  
pp. 191-196
Author(s):  
Yana Viktorovna Smol’nikova ◽  
Nadezhda Aleksandrovna Velichko ◽  
Valentina Leonidovna Bopp ◽  
Aleksandr Vladimirovich Kolomeitsev ◽  
Oksana Valeriyevna Stutko ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Humic Acids ◽  
Oil Content ◽  
Gas Liquid Chromatography ◽  
Brassica Napus L ◽  
Effect Of Treatment ◽  
A Chain

The article analyzes changes in the fatty acid composition of spring rapeseed represented by Trapper and Siberian varieties after their treatment with «Huminatrin» and «Beres-8». The fatty acid composition of spring rapeseed cultivated in the Siberian region was studied by gas-liquid chromatography. The rapeseed contains 19 fatty acids with a chain length of 12 to 24 carbon atoms. The treatment with preparations containing humic acids caused different reactions in varieties. The Trapper variety showed a decrease in oil content after processing, while the Siberian variety showed an increase in oil content. It was found that treatment with «Huminatrin» and «Beres-8» caused an increase in linoleic and linolenic acids and a decrease in oleic fatty acids. The maximum content of linoleic and linolenic fatty acids was observed in seeds after their treatment with the «Huminatrin». Also the treatment with «Huminatrin» and «Beres-8» preparations of Trapper and Siberian varieties led to an increase in the concentration of limited fatty acids.

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