scholarly journals Dose-Dependent Impact of Bee Pollen Supplementation on Macroscopic and Microscopic Structure of Femoral Bone in Rats

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1265
Author(s):  
Monika Martiniakova ◽  
Ivana Bobonova ◽  
Robert Toman ◽  
Branislav Galik ◽  
Maria Bauerova ◽  
...  
Keyword(s):  
Cortical Bone ◽  
Bone Structure ◽  
Group Versus ◽  
Male Rats ◽  
Feed Additive ◽  
Standard Diet ◽  
Microscopic Structure ◽  
Femoral Bone ◽  
Bee Pollen ◽  
The Impact

Bee pollen has been successfully used as a feed additive with beneficial impacts on productive, reproductive, and immune conditions of animals. However, its effect on bone structure and bone health remains controversial. Therefore, the purpose of our study was to examine the impact of bee pollen supplementation on macroscopic and microscopic structure of a femoral bone using rats as suitable animal models. Male rats (1 month-old) were assigned into three groups: control (C group) that was fed a standard diet without bee pollen and two bee pollen supplemented groups (P1 and P2 groups) that received an experimental diet including 0.5% and 0.75% of bee pollen, respectively, for 3 months. A number of unfavorable effects of 0.75% bee pollen administration on bone weight, cortical bone thickness, calcium content, alkaline phosphatase activity, sizes of primary osteons’ vascular canals, Haversian canals and secondary osteons in the cortical bone have been recorded, whereas these bone parameters were significantly decreased in the P2 group versus the C group. On the contrary, the concentration of 0.5% did not affect any of bone features mentioned above. In conclusion, the impact of bee pollen supplementation on femoral bone structure of rats depends on the dose used.

scholarly journals High Caloric Diet Induces Memory Impairment and Disrupts Synaptic Plasticity in Aged Rats

2021 ◽  
Vol 43 (3) ◽  
pp. 2305-2319
Author(s):  
Sara L. Paulo ◽  
Catarina Miranda-Lourenço ◽  
Rita F. Belo ◽  
Rui S. Rodrigues ◽  
João Fonseca-Gomes ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Memory Impairment ◽  
Overweight And Obesity ◽  
Synaptic Function ◽  
Male Rats ◽  
Standard Diet ◽  
Aged Rats ◽  
High Caloric Diet ◽  
The Impact

The increasing consumption of sugar and fat seen over the last decades and the consequent overweight and obesity, were recently linked with a deleterious effect on cognition and synaptic function. A major question, which remains to be clarified, is whether obesity in the elderly is an additional risk factor for cognitive impairment. We aimed at unravelling the impact of a chronic high caloric diet (HCD) on memory performance and synaptic plasticity in aged rats. Male rats were kept on an HCD or a standard diet (control) from 1 to 24 months of age. The results showed that under an HCD, aged rats were obese and displayed significant long-term recognition memory impairment when compared to age-matched controls. Ex vivo synaptic plasticity recorded from hippocampal slices from HCD-fed aged rats revealed a reduction in the magnitude of long-term potentiation, accompanied by a decrease in the levels of the brain-derived neurotrophic factor receptors TrkB full-length (TrkB-FL). No alterations in neurogenesis were observed, as quantified by the density of immature doublecortin-positive neurons in the hippocampal dentate gyrus. This study highlights that obesity induced by a chronic HCD exacerbates age-associated cognitive decline, likely due to impaired synaptic plasticity, which might be associated with deficits in TrkB-FL signaling.

scholarly journals Effects of a single intraperitoneal administration of cadmium on femoral bone structure in male rats

2011 ◽  
Vol 53 (1) ◽  
Author(s):  
Monika Martiniaková ◽  
Hana Chovancová ◽  
Radoslav Omelka ◽  
Birgit Grosskopf ◽  
Róbert Toman
Keyword(s):  
Bone Structure ◽  
Intraperitoneal Administration ◽  
Male Rats ◽  
Femoral Bone

scholarly journals A Novel Automated System Yields Reproducible Temporal Feeding Patterns in Laboratory Rodents

2019 ◽  
Vol 149 (9) ◽  
pp. 1674-1684
Author(s):  
Thomas W Tilston ◽  
Richard D Brown ◽  
Matthew J Wateridge ◽  
Bradley Arms-Williams ◽  
Jamie J Walker ◽  
...  
Keyword(s):  
Food Intake ◽  
Reliable Method ◽  
Male Rats ◽  
Standard Diet ◽  
Feeding Patterns ◽  
Laboratory Rodents ◽  
Meal Feeding ◽  
Ad Libitum ◽  
The Impact ◽  
Cumulative Food Intake

ABSTRACT Background The impact of temporal feeding patterns remains a major unanswered question in nutritional science. Progress has been hampered by the absence of a reliable method to impose temporal feeding in laboratory rodents, without the confounding influence of food-hoarding behavior. Objective The aim of this study was to develop and validate a reliable method for supplying crushed diets to laboratory rodents in consistent, relevant feeding patterns for prolonged periods. Methods We programmed our experimental feeding station to deliver a standard diet [StD; Atwater Fuel Energy (AFE) 13.9% fat] or high-fat diet (HFD; AFE 45% fat) during nocturnal grazing [providing 1/24th of the total daily food intake (tdF/I) of ad libitum–fed controls every 30 min] and meal-fed (3 × 1-h periods of ad libitum feeding) patterns in male rats (Sprague-Dawley: 4 wk old, 72–119 g) and mice [C57/Bl6J wild-type (WT): 6 mo old, 29–37 g], and ghrelin-null littermates (Ghr−/−; 27–34 g). Results Grazing yielded accurate, consistent feeding events in rats, with an approximately linear rise in nocturnal cumulative food intake [tdF/I (StD): 97.4 ± 1.5% accurate compared with manual measurement; R2 = 0.86; tdF/I (HFD): 99.0 ± 1.4% accurate; R2 = 0.86]. Meal-feeding produced 3 nocturnal meals of equal size and duration in StD-fed rats (tdF/I: 97.4 ± 0.9% accurate; R2 = 0.90), whereas the second meal size increased progressively in HFD-fed rats (44% higher on day 35 than on day 14; P < 0.01). Importantly, cumulative food intake in grazing and meal-fed rats was identical. Similar results were obtained in WT mice except that less restricted grazing induced hyperphagia (compared with meal-fed WT mice; P < 0.05 from day 1). This difference was abolished in Ghr−/− mice, with meal initiation delayed and meal duration enhanced. Neither pattern elevated corticosterone secretion in rats, but meal-feeding aligned ultradian pulses. Conclusions We have established a consistent, measurable, researcher-defined, stress-free method for imposing temporal feeding patterns in rats and mice. This approach will facilitate progress in understanding the physiologic impact of feeding patterns.

scholarly journals Effect of Pre- and Post-Weaning High-Fat Dietary Manipulation on Intestinal Microflora and Alkaline Phosphatase Activity in Male Rats

2017 ◽  
pp. 677-685 ◽  
Author(s):  
Z. ŠEFČÍKOVÁ ◽  
D. BUJŇÁKOVÁ
Keyword(s):  
Alkaline Phosphatase ◽  
Intestinal Microbiota ◽  
Alkaline Phosphatase Activity ◽  
Intestinal Microflora ◽  
Male Rats ◽  
Standard Diet ◽  
Fat Intake ◽  
Dietary Manipulation ◽  
High Fat ◽  
The Impact

We investigated the impact of a high-fat (HF) diet during pre- and post-weaning periods on the intestinal microbiota and alkaline phosphatase (AP) activity in male rats. Nutrition from birth was influenced by feeding rat dams with either a standard or HF diet. After weaning male pups nursed by control dams continued on a standard diet (CC) or HF diet (C→HF), while offspring nursed by HF dams continued on HF diet (HF) or standard diet (HF→C). The numbers of Bacteroides/Prevotella (BAC) and Lactobacillus/Enterococcus (LAB) in the gut were determined by FISH technique. HF pups displayed enhanced adiposity and increased AP activity (19 %), as well as higher LAB (P<0.001) and lower numbers of BAC (P<0.001) in the jejunum and colon than controls. In HF→C rats, post-weaning lower fat intake resulted in decreased fat deposition accompanied by reduced AP activity (20 %) compared to HF rats. Composition of the intestinal microbiota in these rats was not influenced. In contrast, in comparison with controls, C→HF rats displayed higher LAB (P<0.001) and lower BAC (P<0.001) together with increased adiposity and AP activity (14 %). These results indicate that consumption of diet with different fat content could modulate gut microbial/functional conditions depending on the period when the nutritional manipulation occurs.

scholarly journals The Impact of Multispecies Probiotics on Calcium and Magnesium Status in Healthy Male Rats

Nutrients ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 3513
Author(s):  
Joanna Suliburska ◽  
Iskandar Azmy Harahap ◽  
Katarzyna Skrypnik ◽  
Paweł Bogdański
Keyword(s):  
Male Rats ◽  
Molar Ratio ◽  
High Dose ◽  
Standard Diet ◽  
Probiotic Properties ◽  
Femur Bone ◽  
Calcium And Magnesium ◽  
Distribution In The Organism ◽  
The Impact ◽  
The Relationship

Although probiotics have been discovered in numerous diseases in the last decade, there is little consensus on the relationship between probiotic properties and minerals balance and their distribution in the organism. This research aimed to evaluate the calcium (Ca) and magnesium (Mg) status in rats on a diet containing multispecies probiotics. Thirty male 10-week-old Wistar rats were selected and divided into three groups (n = 10 rats)—a group fed a standard diet (C), a group fed a low-dose of multispecies probiotics with 2.5 × 109 CFU per day (LD), and a group fed high-dose of multispecies probiotics 1 × 1010 CFU per day (HD) for 6 weeks. The results revealed that HD intake significantly increased the Ca concentration in hair and Mg concentration in femur bones. A significant positive correlation was found between calcium and magnesium levels in hair. The Ca/Mg molar ratio was lower in testicles in the groups with probiotics. In conclusion, multispecies probiotics altered the Ca concentration in hair and Mg level in femur bone, and also changed the molar ratio of these elements in testicles in male rats.

The effect of bee pollen on femoral bone structure in adult rats

2014 ◽  
Vol 185 ◽  
pp. S79
Author(s):  
Ivana Bobonova ◽  
Monika Martiniakova ◽  
Radoslav Omelka ◽  
Hana Chovancova ◽  
Robert Toman
Keyword(s):  
Bone Structure ◽  
Femoral Bone ◽  
Adult Rats ◽  
Bee Pollen

scholarly journals Effects of bee bread, Cornelian cherries treatment on the femoral bone structure using Zucker diabetic fatty rats as an animal model

2021 ◽  
Author(s):  
M Martiniakova ◽  
J Blahova ◽  
V Kovacova ◽  
V Mondockova ◽  
R Babosova ◽  
...  
Keyword(s):  
Trabecular Bone ◽  
Cortical Bone ◽  
Bone Structure ◽  
Relative Volume ◽  
Bone Surface ◽  
Femoral Bone ◽  
Trabecular Thickness ◽  
Zucker Diabetic Fatty Rats ◽  
Bee Bread ◽  
Zdf Rats

: This is a pilot study dealing with the reduced femoral bone structure in Zucker diabetic fatty (ZDF) rats following a 10 week treatment with bee bread and Cornelian cherries. The adult ZDF rats were divided into 4 groups: the E1 group received bee bread (500 mg/kg b.w. daily), the E2 group received Cornelian cherries (500 mg/kg b.w. per day), the E3 group was simultaneously supplemented by bee bread and Cornelian cherries (500 + 500 mg/kg b.w. daily), and the C group served as an untreated diabetic control. A non-significant decrease in the blood glucose, total cholesterol, triglyceride concentrations, ALP activity and a non-significant increase in the insulin, total body weight of the ZDF rats, femoral length and weight were observed in the E1, E2, and E3 groups. The treatments had no impact on the relative volume of the cortical bone, bone mineral density, bone surface and cortical bone thickness. On the contrary, the relative volume of the trabecular bone, trabecular thickness and trabecular bone surface were significantly increased in the E1 group. The relative volume of the trabecular bone and trabecular thickness were significantly elevated in the E2 group. In the E3 group, the trabecular thickness was found to have significantly increased. Single administrations of either bee bread or Cornelian cherries had more positive effects on the trabecular bone microarchitecture in the ZDF rats than the simultaneous supplementation.

Influence of DAFS-25+polizon composition on blood and functional state of liver of steers at fattening

2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  
Keyword(s):  
Functional State ◽  
White Blood Cells ◽  
Live Weight ◽  
The Body ◽  
Farm Animals ◽  
Control Group ◽  
Standard Diet ◽  
The Impact ◽  
Experimental Group ◽  
Stimulation Of

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.

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