scholarly journals Platelet-Rich Plasma for the Treatment of Degenerative Lumbosacral Stenosis: A Study with Retired Working Dogs

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2965
Author(s):  
Ángel María Hernández-Guerra ◽  
José María Carrillo ◽  
Joaquín Jesús Sopena ◽  
José Manuel Vilar ◽  
Pau Peláez ◽  
...  
Keyword(s):  
Growth Factors ◽  
Structural Integrity ◽  
Platelet Rich Plasma ◽  
Cauda Equina ◽  
Epidural Injection ◽  
Good Alternative ◽  
Epidural Injections ◽  
Tissue Healing ◽  
Before And After ◽  
Working Dogs

Traditionally, canine degenerative lumbosacral stenosis (DLS) has been defined as a multifactorial syndrome characterized by lumbosacral pain triggered by the compression of the nerve rootlets of the cauda equina. There is still no consensus on the treatment of this condition, probably because there are a plethora of possible causes. In addition to compression, inflammation is a very important factor in the physiopathology of the disorder. Platelet-rich plasma (PRP) consists of an increased concentration of autologous platelets suspended in a small amount of plasma. Platelets are a source of several growth factors. Growth factors were shown to help in wound healing and biological processes, such as chemotaxis, neovascularization and synthesis of extracellular matrix, and growth factors were used to improve soft tissue healing and bone regeneration. PRP also facilitates the restoration of the structural integrity of the affected anatomy. Fourteen dogs diagnosed with DLS were treated with three epidural injections of PRP on days 0, 15 and 45. All dogs showed clinical improvement 3 months after the initial treatment. Gait was also objectively assessed by means of the use of force platform analysis before and after treatment, showing significant improvement. The results show that PRP may provide a good alternative to other nonsurgical treatments, such as prednisolone epidural injection.

scholarly journals Changes in the activity of ovine blood-derived macrophages stimulated with antimicrobial peptide extract (AMP) or platelet-rich plasma (PRP)

2019 ◽  
Vol 63 (2) ◽  
pp. 235-242
Author(s):  
Joanna Wessely-Szponder ◽  
Tomasz Szponder ◽  
Ryszard Bobowiec ◽  
Joanna Michalska
Keyword(s):  
Antimicrobial Activity ◽  
Growth Factors ◽  
Soft Tissue ◽  
Platelet Rich Plasma ◽  
Innate Immune ◽  
Ros Generation ◽  
Soft Tissue Healing ◽  
Tissue Healing ◽  
Functional Features ◽  
Stimulation Of

AbstractIntroduction: Antimicrobial peptides (AMP) are a large group of innate immune effectors, which apart from antimicrobial activity show immunomodulative properties. Platelet-rich plasma (PRP) is a source of autologous growth factors and is used for stimulation of bone and soft tissue healing. The purpose of this study was to assess the influence of PRP and AMP extract on ovine monocyte-derived macrophage cultures. Material and Methods: The study was conducted on ovine macrophages (Mfs) previously stimulated with LPS or dexamethasone and then with preparations of PRP or AMP. Following activation of the Mfs their morphological and functional features were assessed. Results: The study revealed pro-inflammatory influence of both examined preparations on Mfs cultures on the basis of morphology, ROS generation and arginase activity. Both preparations enhanced the pro-inflammatory response of cultured Mfs. Conclusion: This activity may intensify the antimicrobial action of Mfs, however, in cases of excessive and prolonged inflammation the use of these preparations should be limited.

scholarly journals REPARATIVE PROCESSES FEATURES IN TROPHIC ULCERS CAUSED BY DIABETES MELLITUS WITH THE USE OF PLATELET-RICH PLASMA

2020 ◽  
Vol 73 (10) ◽  
pp. 2150-2155
Author(s):  
Igor D. Duzhiy ◽  
Andrii S. Nikolaienko ◽  
Vasyl M. Popadynets ◽  
Oleksandr V. Kravets ◽  
Igor Y. Hresko ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Growth Factors ◽  
Comparison Group ◽  
Platelet Rich Plasma ◽  
Main Group ◽  
Lower Limbs ◽  
Before And After ◽  
Autologous Platelet Rich Plasma ◽  
Inflammatory Changes ◽  
Autologous Platelet

The aim: Was following: the improvement of the treatment results of the lower limbs ulcers, caused by the diabetes mellitus by using our technique of the platelet-rich plasma application; the study of the features of the morphological and immunohistochemical changes, and the effect of the growth factors of the platelet-rich plasma on the regeneration and healing of the ulcers. Materials and methods: 38 patients with the trophic ulcers of the lower limbs, caused by diabetes mellitus were involved in the study. To assess the morphological features of the reparative processes before and after the treatment with PRP, the histological and immunohystochemical studies of the biopsy specimen of ulcers were carried out. Results: The total epithelialization of the ulcers in the patients from the main group was achieved on 46.5 day, and in the comparison group – 81.7 day. The light and optical study of the specimen of the main group, using the autologous platelet-rich plasma revealed the elimination of the destructive changes and reduction of the mixed-cellular infiltration compared to the patients from the comparison group. The immunohystochemical study revealed the increase of the growth factors receptors. Conclusions: The usage of the autologous platelet-rich plasma in the treatment of the trophic ulcers results in the rapid regeneration by the decrease of the destructive and inflammatory changes as well as the improvement of the vascularization.

scholarly journals Skin Grafts Associated with Platelet Rich Plasma and Surgical Sponge - Literature Review

2017 ◽  
Vol 9 (4) ◽  
pp. 51
Author(s):  
Josiane M. Pazzini ◽  
Eduardo L. Serafim ◽  
Ricardo R. A. Uscategui ◽  
Vivian T. Almeida ◽  
Carlos A. C. Oliva ◽  
...  
Keyword(s):  
Growth Factors ◽  
Literature Review ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Skin Grafts ◽  
Vascular Pedicle ◽  
Surgical Sponge ◽  
Donor Region ◽  
Tissue Healing ◽  
Surgical Wounds

Skin graft is one of the techniques used to reconstruct surgical wounds. The graft is composed of epidermal and dermal segments that are completely removed from the donor region and transferred to the recipient bed. After its implantation it’s recommended to make compressive dressing in the receiver bed. Since the grafts do not have a vascular pedicle, it’s important to make the compressive dressing to improve graft contact with the wound and allow adequate angiogenesis. The compressive dressing is made with a sponge or foam, which offers adequate protection and reduces the discomfort of the patient in the postoperative period. The use of platelet-rich plasma (PRP) is the objective of several studies associated with the reduction of postoperative surgical complications, especially necrosis. This product is a result of the centrifugation of the patient’s blood that promotes the separation of its constituents and allows the extraction of plasma with higher concentration of platelets. PRP improves the tissue healing process by releasing biological mediators and growth factors at the site of application. Researches on platelet-rich plasma used in reconstructive surgery are important because this product has therapeutic characteristics to promote healing. When it’s used in skin grafts, platelet-rich plasma is able to improve graft integration in the recipient bed, and reduce necrosis after the surgical procedure. The use of postoperative surgical sponges associated with platelet-rich plasma is indicated to improve the healing of the graft and to avoid its displacement of the recipient bed.

scholarly journals Application of Platelet Rich Fibrin, Platelet Rich Plasma, Plasma Rich Growth Factors in Different Fields of Dentistry: An Overview

2020 ◽  
pp. 12-23
Author(s):  
Geetanjali Singh ◽  
Bhawna Kumari ◽  
Harangad Singh Grover ◽  
Akriti Mahajan ◽  
Farhat Jabeen ◽  
...  
Keyword(s):  
Growth Factors ◽  
Soft Tissue ◽  
Plasma Proteins ◽  
Bone Growth ◽  
Platelet Rich Plasma ◽  
Platelet Rich Fibrin ◽  
Soft Tissue Regeneration ◽  
Tissue Healing ◽  
Transversal Field ◽  
Potential Use

A huge number of studies have demonstrated that platelet-rich preparations applied to surgical sites, injuries, or wounds are effective way to promote soft tissue healing and bone growth. The potential use of  platelet rich preparations like Platelet - rich plasma and Platelet - rich fibrin are new boon to dentistry termed under the general acronyms PRP (Platelet-Rich Plasma) or PRF (Platelet-Rich Fibrin), is an important current transversal field of research across many fundamental and clinical disciplines.The third important is PRGF.  Plasma Rich in Growth Factor (or PRGF) is a type of plasma enriched of proteins and circulating growth factors able to aid the bone and soft tissue regeneration. PRGF includes plasma proteins and coagulative factors and is then more valuable compared to PRP. There are many scientific literature which cover one or other concise aspect of platelet rich preparation. This review aims at covering all basic definitions, key element, history, preparation, differences between PRF and PRP and applications in a simplified manner.

scholarly journals Relevant Aspects of Centrifugation Step in the Preparation of Platelet-Rich Plasma

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Amanda G. M. Perez ◽  
José Fábio S. D. Lana ◽  
Ana Amélia Rodrigues ◽  
Angela Cristina M. Luzo ◽  
William D. Belangero ◽  
...  
Keyword(s):  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Centrifugal Acceleration ◽  
Platelet Recovery ◽  
Acceleration Time ◽  
Biological Responses ◽  
Tissue Healing ◽  
Healthy Donors ◽  
Platelet Concentration ◽  
Centrifugation Step

Introduction. Platelet-Rich Plasma (PRP) is rich in growth factors, playing important role in tissue healing. The wide variation of reported protocols for preparation of PRP leads to variable compositions, which induce different biological responses and prevent results comparison. This study aims to highlight relevant aspects of the centrifugation step to obtain reproducible results and overall quality. Material and Methods. Samples of blood were collected from 20 healthy donors that have signed free informed consent. Two centrifugation steps (spins) were analyzed for the influence of centrifugal acceleration, time, processed volume, and platelet gradient. The Pure Platelet-Rich Plasma (P-PRP) was characterized as platelet concentration, integrity, and viability (sP-selectin measurement). Results. Lower centrifugal accelerations favour platelet separation. The processing of 3.5 mL of blood at 100 ×g for 10 min (1st spin), 400 ×g for 10 min (2nd spin), withdrawing 2/3 of remnant plasma, promoted high platelet recovery (70–80%) and concentration (5x) maintaining platelet integrity and viability. The recovery of platelets was reduced for a larger WB volume (8.5 mL) processed. Conclusion. Centrifugal acceleration, time, WB processed volume, and minimization of the platelet gradient before sampling are relevant aspects to ensure reproducible compositions within the autologous nature of PRP.

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

scholarly journals Use of PRP, PRF and CGF in Periodontal Regeneration and Facial Rejuvenation—A Narrative Review

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 317
Author(s):  
Eitan Mijiritsky ◽  
Haya Drora Assaf ◽  
Oren Peleg ◽  
Maayan Shacham ◽  
Loredana Cerroni ◽  
...  
Keyword(s):  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Periodontal Regeneration ◽  
Clinical Work ◽  
Gingival Recession ◽  
Vital Role ◽  
Facial Rejuvenation ◽  
Cochrane Library ◽  
The Past ◽  
Autologous Platelet

Growth factors (GFs) play a vital role in cell proliferation, migration, differentiation and angiogenesis. Autologous platelet concentrates (APCs) which contain high levels of GFs make them especially suitable for periodontal regeneration and facial rejuvenation. The main generations of APCs presented are platelet-rich plasma (PRP), platelet-rich fibrin (PRF) and concentrated growth factor (CGF) techniques. The purpose of this review is to provide the clinician with an overview of APCs’ evolution over the past decade in order to give reliable and useful information to be used in clinical work. This review summarizes the most interesting and novel articles published between 1997 and 2020. Electronic and manual searches were conducted in the following databases: Pubmed, Scopus, Cochrane Library and Embase. The following keywords were used: growth factors, VEGF, TGF-b1, PRP, PRF, CGF and periodontal regeneration and/or facial rejuvenation. A total of 73 articles were finally included. The review then addresses the uses of the three different techniques in the two disciplines, as well as the advantages and limitations of each technique. Overall, PRP is mainly used in cases of hard and soft tissue procedures, while PRF is used in gingival recession and the treatment of furcation and intrabony defects; CGF is mainly used in bone regeneration.

scholarly journals Water Quality and Financial Analysis of a System Combining Rainwater and Greywater in a House

Water ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 930
Author(s):  
Gustavo Rosa ◽  
Enedir Ghisi
Keyword(s):  
Water Consumption ◽  
Potable Water ◽  
Treatment Process ◽  
Financial Analysis ◽  
Good Alternative ◽  
Water Savings ◽  
Financial Benefits ◽  
Before And After ◽  
Local Water ◽  
Water Tariffs

This study aimed to evaluate the installation of a system that combines rainwater to wash clothes and greywater to flush toilets in a house located in Florianópolis, southern Brazil. The evaluation consisted of determining the potable water savings, performing a financial analysis, and verifying the possibility of using rainwater and reusing greywater according to the local legislation. The reduction in potable water consumption was determined by comparing the monthly water and sewage bill, before and after installing the system, combining rainwater and greywater. The financial analysis was performed considering the financial benefits, operational costs, costs for installing the system, and local water tariffs. After installing the system, there was a decrease of 38.0% in the monthly potable water consumption, which represents a decrease of 7.00 m3 in the monthly potable water consumption. The payback periods for the old and new water tariffs were equivalent to, respectively, ten years and seven months, and eight years and five months. Both rainwater and greywater had parameters with concentrations higher than the limits established by the legislation. Therefore, the rainwater had to go through a device for discarding the first flush and needed disinfection; and the greywater needed to go through a treatment process. The results indicated that the system combining rainwater and greywater shows significant potential for potable water savings, which can be a good alternative to the preservation of potable water resources. It is important to note that current studies on rainwater use and greywater reuse are mainly related to non-potable purposes, but it was shown herein that rainwater can be used for washing clothes.

Clinical Efficacy of Platelet-Rich Plasma Injection and Its Association With Growth Factors in the Treatment of Mild to Moderate Knee Osteoarthritis: A Randomized Double-Blind Controlled Clinical Trial As Compared With Hyaluronic Acid

2021 ◽  
Vol 49 (2) ◽  
pp. 487-496
Author(s):  
Yong-Beom Park ◽  
Jun-Ho Kim ◽  
Chul-Won Ha ◽  
Dong-Hyun Lee
Keyword(s):  
Hyaluronic Acid ◽  
Growth Factors ◽  
Knee Osteoarthritis ◽  
Clinical Outcomes ◽  
Clinical Efficacy ◽  
Global Assessment ◽  
Platelet Rich Plasma ◽  
Patient Global Assessment ◽  
Symptomatic Knee Osteoarthritis ◽  
Symptomatic Knee

Background: Although platelet-rich plasma (PRP) has potential as a regenerative treatment for knee osteoarthritis, its efficacy varies. Compositional differences among types of PRP could affect clinical outcomes, but the biological characterization of PRP is lacking. Purpose: To assess the efficacy of intra-articular PRP injection in knee osteoarthritis as compared with hyaluronic acid (HA) injection and to determine whether the clinical efficacy of PRP is associated with its biological characteristics. Study Design: Randomized controlled trial; Level of evidence, 1. Methods: A total of 110 patients with symptomatic knee osteoarthritis received a single injection of leukocyte-rich PRP (1 commercial kit) or HA. Clinical data were assessed at baseline and at 6 weeks and 3 and 6 months after injection. The primary endpoint was an improvement in the International Knee Documentation Committee (IKDC) subjective score at 6 months, and the secondary endpoints were improvements in scores based on the Patient Global Assessment, the visual analog scale (VAS) for pain, the Western Ontario and McMaster Universities Osteoarthritis Index, and the Samsung Medical Center patellofemoral score. Cell counts and concentrations of growth factors and cytokines in the injected PRP were assessed to determine their association with clinical outcomes. Results: PRP showed significantly improvement in IKDC subjective scores at 6 months (11.5 in the PRP group vs 6.3 in the HA group; P = .029). There were no significant differences between groups in other clinical outcomes. The Patient Global Assessment score at 6 months was better in the PRP group ( P = .035). The proportion of patients who scored above the minimal clinically important difference (MCID) for VAS at 6 months was significantly higher in the PRP group ( P = .044). Within the PRP group, the concentrations of platelet-derived growth factors were high in patients with a score above the MCID for VAS at 6 months. The incidence of adverse events did not differ between the groups ( P > .05). Conclusion: PRP had better clinical efficacy than HA. High concentrations of growth factors were observed in patients who scored above the MCID for clinical outcomes in the PRP group. These findings indicate that concentration of growth factors needs to be taken into consideration for future investigations of PRP in knee osteoarthritis. Registration: NCT02211521 (ClinicalTrials.gov identifier).

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