scholarly journals Impacts of Rumen Degradable or Undegradable Protein Supplementation with or without Salt on Nutrient Digestion, and VFA Concentrations

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3011
Author(s):  
Marley Manoukian ◽  
Timothy DelCurto ◽  
Janessa Kluth ◽  
Tanner Carlisle ◽  
Noah Davis ◽  
...  
Keyword(s):  
Protein Supplementation ◽  
Neutral Detergent Fiber ◽  
Delivery Method ◽  
Flow Rates ◽  
Ruminal Ph ◽  
Nutrient Digestion ◽  
Rumen Undegradable Protein ◽  
Dietary Treatments ◽  
Supplement Intake ◽  
Degradable Protein

The objectives of this study were to evaluate the effect of differences in protein type and delivery method on rumen dynamics and nutrient digestion. Cows were allotted to rumen degradable protein (RDP) or rumen undegradable protein (RUP) and self-fed (SF) salt-limited pressed blocks or hand-fed (HF) loose supplement, resulting in four dietary treatments. There was a delivery effect (p = 0.04) on neutral detergent fiber (NDF) intake, as the SF animals consumed more NDF than HF animals. The RDP-SF animals had greater NDF digestibility (p = 0.04) and water intake (p = 0.03) than the three other treatments. Supplement intake displayed a protein type effect (p = 0.03), as RDP-supplemented animals consumed more supplement on a g·kg body weight (BW)−1 d−1 basis than RUP animals. There was an effect of protein type (p = 0.02) and delivery method (p = 0.03) on fluid flow rate, with RUP and HF cows having greater liquid flow rates. Ruminal pH was lower (p < 0.01) in RDP-HF cows than RDP-SF cows at all hours, except 4-h post-feeding. RDP-SF animals had the greatest (p < 0.01) concentrations of ruminal ammonia. Valerate ruminal concentrations were greater (p = 0.04) in RDP supplemented animals compared to RUP supplemented animals. In conclusion, self-fed supplements containing RDP may enhance the use of low-quality forages and increase ruminal ammonia concentrations.

scholarly journals USE OF VIRGINIAMYCIN AND SALINOMYCIN IN THE DIET OF BEEF CATTLE REARED UNDER GRAZING DURING THE RAINY SEASON: PERFORMANCE AND RUMINAL METABOLISM

2019 ◽  
Vol 20 ◽  
Author(s):  
Sérgio Fernandes Ferreira ◽  
Juliano José de Resende Fernandes ◽  
João Teodoro Padua ◽  
Ubirajara Oliveira Bilego ◽  
Marcondes Dias de Freitas Neto ◽  
...  
Keyword(s):  
Rainy Season ◽  
Average Daily Gain ◽  
Neutral Detergent Fiber ◽  
Symbiotic Interaction ◽  
Mineral Supplement ◽  
Ruminal Ph ◽  
Dry Matter Digestibility ◽  
Biometric Measurements ◽  
Daily Gain ◽  
Supplement Intake

Abstract The rumen is the most studied organ with regard to the symbiotic interaction between host and microbiota in the digestive tract of ruminants. Thus, this study aimed at evaluating the effects of including virginiamycin and salinomycin to the supplement for cattle fed tropical grass diet during the rainy season. Three treatments were set: control - mineral supplement COMIGO - Cria 61-F2 (MS); virginiamycin - MS + virginiamycin (Phigrow (r)) 100 mg/animal/day; salinomycin - MS + salinomycin (Posistac(r)) 108 mg/animal/day for the experiment 1 (performance) and experiment 2 (ruminal metabolism). The control showed higher Mineral supplement intake (MSI) (P <0.05), the virginiamycin had higher average daily gain (ADG) (P> 0.05) and better financial efficiency. Biometric measurements showed no differences (P> 0.05), suggesting a change in the ADG composition. There was no difference (P> 0.05) for dry matter digestibility (DMDIS), acid detergent fiber digestibility (ADFDIS), ruminal pH and ammoniacal-N. The virginiamycin had the highest effective neutral detergent fiber (NDF) degradability (P> 0.05) in the passage rates of 2 and 5%; the degradability rates were similar to control treatement and higher than that of salinomycin at the rate of 8%/hour. The virginiamycin or salinomycin can be conveyed to the MS, but do not promote significant effects on ruminal pH, ammoniacal-N, DMDIS and ADFDIS; but virginiamycin promoted greater effective degradability of NDF.

251 Effects of Roughage Source on Rumination Time and Ruminal Ph in Steers Fed a Steam-flaked Corn Finishing Diet

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 127-127
Author(s):  
Wyatt Smith ◽  
Catherine Lockard ◽  
Caleb Lockard ◽  
Megan Homolka ◽  
Kendall Karr ◽  
...  
Keyword(s):  
Latin Square ◽  
Neutral Detergent Fiber ◽  
Beef Steers ◽  
Finishing Diets ◽  
Ruminal Ph ◽  
Penn State ◽  
Dietary Treatments ◽  
Particle Separator ◽  
Finishing Beef Cattle ◽  
Different Sources

Abstract Research is limited on how physically effective fiber from various roughage sources aids in rumination time and ruminal pH of finishing beef cattle. This experiment’s objective was to evaluate rumination time and ruminal pH of beef steers consuming finishing diets with varying roughage sources (corn stalks, cotton burrs, or wheat silage). We hypothesized that roughage type would not impact rumination time and ruminal pH if different sources provide similar dietary neutral detergent fiber (NDF). Ruminally cannulated steers (n = 6; average BW = 644.56 + 13.15 kg) were used in a 3 × 3 replicated Latin square with 3 dietary treatments and 3, 21-d periods (20-d diet adaptation, 1-d sampling). Steers consumed a steam-flaked corn-based finishing diet containing corn stalks (CS), cotton burrs (CB), or wheat silage (WS) included at 7% (DM basis) of the diet. Dietary NDF was similar across treatments. Steers were fitted with a sensory collar to record daily rumination (Allflex Livestock Intelligence). Ruminal pH was measured using a handheld pH probe on d-21 at 0, 3, 6, 12, 24 hr postprandial. The NDF and dry matter intake (DMI) were greatest for steers consuming the WS diet (P &lt; 0.01) while CS and CB diets did not differ (P = 0.81). While dietary NDF and estimated physically effective NDF (peNDF) were similar among roughage sources, CB had the lowest actual peNDF, consistent with lower rumination time (P &lt; 0.01) and lower ruminal pH (P = 0.29). This experiment’s results indicate that roughage source impacted rumination time despite feeding steers a steam-flaked corn-based finishing diet with similar roughage and NDF levels. Using rumination time (min/d) to determine peNDF was a better indicator of rumen function parameters than particle size measured via the Penn State Particle Separator equation.

scholarly journals Effects of dietary NFC/NDF on rumen microbiomes of Karakul sheep based on Three Generations of Full-length Amplifiers sequencing

2019 ◽  
Author(s):  
Xuanxuan Pu ◽  
Xuefeng Guo ◽  
Chenyu Jiang ◽  
Junfeng Liu ◽  
Xiuping Zhang ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Neutral Detergent Fiber ◽  
Ruminal Bacteria ◽  
Ruminal Ph ◽  
Genus Level ◽  
Group 3 ◽  
The Difference ◽  
Dietary Treatments ◽  
Dominant Bacteria ◽  
Group 1

AbstractAn study was was conducted to investigate the effects of dietary(non fibrous carbohydrate) NFC/(neutral detergent fiber)NDF on ruminal bacteria in Karakul sheep. Twelve Karakul sheep were assigned randomly to four dietary treatments of NFC/NDF (0.78, 1.23, 1.61 and 2.00 respectively) as group 1, 2, 3 to 4. The experiment lasted for four periods, period I (1~18 d), II (19~36 d), III (37~54 d) and IV (55~72 d). Ruminal digesta were collected consecutively for three days to measure pH and bacteria per period. The results indicated that the average ruminal pH and amounts of OTUs were decreased with the increase of dietary NFC/NDF for four periods. At phylum level, Bacteroidetes and Firmicutes were the predominant bacteria of four periods, Bacteroidetes were decreased, while the relative abundance of Firmicutes was increased with dietary NFC/NDF for four periods, but the difference wasn’t significant (P>0.05). At genus level, the most relative abundance genus was unidentified-Lachnospiraceae which reached the highest in group 3 for four periods, but the difference wasn’t significant (P>0.05). Conclusion: ruminal pH and bacteria were decreased with the increase of dietary NFC/NDF and the most dominant bacteria were not change with dietary NFC/NDF and periods in Karakul sheep.

scholarly journals PSX-37 Effect of forage neutral detergent fiber level for feedlot lambs: Digestibility

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 448-449
Author(s):  
Josue Godinez ◽  
Francisco Castrejón-Pineda ◽  
Alejandro Plascencia ◽  
Águeda García-Pérez ◽  
Luis Corona
Keyword(s):  
Energy Content ◽  
Latin Square ◽  
Optimal Level ◽  
Neutral Detergent Fiber ◽  
Digestible Energy ◽  
Finishing Diets ◽  
Ruminal Ph ◽  
Feedlot Lambs ◽  
Dietary Treatments ◽  
Corn Grain

Abstract Fiber plays an important role to maintain the normal functioning of the rumen in cattle fed with high-concentrate diets. However, the optimal incorporation of fiber in the finishing diets should counteract the two main limitations of this ingredient in fattening due to the limitation in the ingestion of the diet and the dilution of diet energy as result to its low energy content. Therefore, efforts have been made to determine the optimal level of NDF in the finishing diets where the negative associative effects of the NDF are avoided without undermining its role as a functional feed in fattening. For the above, eight lambs (30.50± 4.44 kg initial weight) were used in a replicated 4 × 4 Latin square design in order to evaluate effective neutral detergent fiber (eNDF) levels (4, 8, 12, and 16% diet DM) on growing-finishing diets in lambs. Experiment had 4 period of 21 days each (17-d to diet adaptation and 4-d to sampling). Dietary treatments consisted of a cracked corn-based diet in which the eNDF concentration of diet was manipulated by replacing cracked corn grain by corn stubble. As the level of eNDF increased, intake of DM, MO, NDF, and GE were linearly increased (P &lt; 0.01), but starch intake was decreased. The apparent digestible energy (Mcal/kg) showed a quadratic effect (P &lt; 0.01) being maximal at 8% eNDF level increased. Ruminal pH increased (P &lt; 0.01) from 5.77 to 6.70 as the level of eNDF increased. It is concluded that 4% of eNDF level in corn-cracked based finishing diet is enough so as not affect ruminal health in lambs. Digestible energy was optimized at 8% of eNDF level.

Effects of Increasing Rumen‐Undegradable Protein Supplementation Levels on Early Weaned Calves Grazing Stargrass

Crop Science ◽  
2013 ◽  
Vol 53 (1) ◽  
pp. 322-328 ◽  
Author(s):  
J. M. B. Vendramini ◽  
J. D. Arthington ◽  
L. E. Sollenberger
Keyword(s):  
Protein Supplementation ◽  
Rumen Undegradable Protein

PSX-B-10 Effect of undigested neutral detergent fiber concentration and forage inclusion rate on ruminal pH, reticular motility, and total tract permeability for finishing beef heifers

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 457-458
Author(s):  
Murillo C Pereira ◽  
Karen A Beauchemin ◽  
Tim A McAllister ◽  
Wenzhu Yang ◽  
Joyce Van Donkersgoed ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Dry Matter ◽  
Latin Square ◽  
Barley Grain ◽  
Neutral Detergent Fiber ◽  
Fiber Concentration ◽  
Eating Time ◽  
Ruminal Ph ◽  
Contraction Duration ◽  
Gastrointestinal Permeability

Abstract This study evaluated the effects of undigested neutral detergent fiber (uNDF) concentration and forage inclusion (FI) rate on dry matter (DM) intake, ruminal pH, reticular contractions, and gastrointestinal permeability for finishing beef cattle. Five ruminally cannulated Hereford′Simmental heifers (699±69.1 kg) were used in an incomplete 6×6 Latin square (26-d periods) with a 2×3 factorial treatment arrangement. Barley grain-based diets were formulated using barley silage or wheat straw to provide low or high uNDF (7.1 vs. 8.5% DM) with forage proportions of 5, 10, or 15% of dietary DM. Dry matter intake (P ≥ 0.10) and eating time (P ≥ 0.13) were not affected by uNDF, FI, or uNDF′FI. With low uNDF diets, increasing FI numerically (P = 0.02) increased rumination time (min/d); while, with high uNDF diets, rumination time increased with 5 to 10% FI, but not thereafter (P = 0.03). Mean ruminal pH was not affected by uNDF (6.17 vs. 6.19; P = 0.08), but increased with increasing FI (6.04b, 6.23a, and 6.28a; P = 0.02). Duration of ruminal pH &lt; 5.5 was not affected by uNDF but tended (P = 0.07) to be reduced with increasing FI. High uNDF diet tended to increase the frequency of reticular contractions (1.43 vs. 1.51 contractions/min; P = 0.07) but decreased the contraction duration (13.2 vs. 14.1 sec; P = 0.04). Increasing FI increased contraction frequency (1.39b, 1.50a, and 1.53a contractions/min; P = 0.03) and tended to reduce contraction duration as forage increased from 5 to 10 and 15% (14.3, 13.1, and 13.6 sec; P = 0.07). Feeding high uNDF decreased (P = 0.05) permeability of the gastrointestinal tract based on the appearance of Cr-EDTA in urine following an intra-ruminal dose. Increasing FI tended to reduce gastrointestinal tract permeability (P = 0.06). Limited interactions indicate that uNDF and FI act independently suggesting that increasing dietary uNDF, without increasing FI rate, can stimulate frequency of reticulo-ruminal contractions and reduce gastrointestinal permeability for finishing cattle.

scholarly journals Ruminal parameters and ruminal degradability of feedlot sheep fed safflower grains

2018 ◽  
Vol 19 (3) ◽  
pp. 324-335
Author(s):  
Jefferson Leonardo Rocha Alves ◽  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Antônio Campanha Martinez ◽  
Aguinaldo Yoshio Nakamura ◽  
Jefferson Rodrigues Gandra ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Dry Matter ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
Asymptotic Model ◽  
Ruminal Fermentation ◽  
Mean Values ◽  
Ruminal Ph ◽  
Significant Difference ◽  
The Times

SUMMARY This study aimed to evaluate the rumen degradability of dry matter (DM), disappearance of neutral detergent fiber (NDF) of Tifton 85 hay, and fermentation parameters (pH and ruminal ammonia nitrogen) of sheep fed diets with inclusion of safflower grains (0, 7.50 and 15%). Three mixed breed lambs with average body weight of 35.50± 1.50 kg were used. The rumen degradability of dry matter and NDF of the Tifton 85 haywas analyzedby the disappearance of DM and NDF, and the kinetic parameters adjusted according to the first order asymptotic model. The samples were incubated in the rumen in descending order (96, 48, 24, 12, 6, 3, 0 hours). The determination of ruminal pH and ruminal ammonia nitrogen (RAN) was performed before feeding and 2, 4, 6 and 8 hours after feeding. Data of ruminal fermentation were tested by analysis of variance with repeated measures in time, at 5% probability. Ruminal pH values were not statistically different. When evaluating the RAN, there was a significant difference between the times and treatments for the diet with 15% safflower. The diets presented low effective degradability of DM and NDF with mean values of 18.02% and 47.40%, respectively. The safflower grain can be used up to 7.50% in the diets without altering the degradability and ruminal fermentation.

330 3-nitrooxypropanol Supplementation of a Forage Diet Decreased Enteric Methane Emissions from Beef Cattle Without Affecting Apparent Total-tract Digestibility

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 186-187
Author(s):  
Aklilu Alemu ◽  
Xiu Min Zhang ◽  
Maik Kindermann ◽  
Karen A Beauchemin
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Control Diet ◽  
Nutrient Digestibility ◽  
Ch4 Emissions ◽  
Ruminal Ph ◽  
Enteric Methane ◽  
Ruminant Diets ◽  
Dietary Treatments ◽  
High Forage

Abstract Supplementation of ruminant diets with the investigational methane (CH4) inhibitor 3-nitrooxypropanol (3-NOP; DSM Nutritional Products, Switzerland) is as a promising mitigation strategy. However, most studies have examined high grain or mixed forage-concentrate diets. The objective of this study was to evaluate the effects of supplementing a forage diet with 3-NOP on rumen fermentation, CH4 emissions and apparent total-tract nutrient digestibility. Eight ruminally cannulated beef heifers (BW = 514 kg) were randomly allocated to two treatments in a crossover design with 49-d periods. Dietary treatments were: control, high-forage diet (90% forage DM basis) without 3-NOP; and NOP, control diet supplemented with 150 mg 3-NOP/kg DM. After a 14-d diet adaption, dry matter (DM) intake (DMI) was recorded daily. Rumen contents were collected on days 17 and 28 for volatile fatty acid (VFA) analysis, whereas ruminal pH was continuously monitored from day 20 to 27. Diet digestibility was measured on day 38 to 41 by total collection of feces. Enteric CH4 emissions were measured on days 46 to 49 in chambers. Dry matter intake was lower (P = 0.001) for NOP (10.0 kg/d) as compared with control (10.2 kg/d). However, DM, neutral and acid detergent fiber, and crude protein digestibilities were similar between treatments (P ≥ 0.29). Although total VFA concentration was not affected by treatment (P = 0.19), the reduction in acetate and increase in propionate proportion for NOP lowered (P &lt; 0.001) the acetate to propionate ratio by 18% as compared with control. Mean pH was lower (P = 0.03) for control (6.46) than NOP (6.57). Furthermore, CH4 yield (g/kg DMI) was 21.6% less (P &lt; 0.001) for NOP relative to control (25.5 g/kg DMI). Overall, the results indicate that enteric CH4 emissions were effectively decreased with 3-NOP supplementation of a forage diet without affecting apparent total-tract digestibility of nutrients.

scholarly journals Effect of stage of maturity at harvest for forage pea (Pisum sativum L.) on eating behavior, ruminal fermentation, and digestibility when fed as hay to yearling beef heifers

2019 ◽  
Vol 4 (1) ◽  
pp. 149-158 ◽  
Author(s):  
Alex A Pursley ◽  
Bill Biligetu ◽  
Tom Warkentin ◽  
Herbert A Lardner ◽  
Gregory B Penner
Keyword(s):  
Pisum Sativum ◽  
Eating Behavior ◽  
Latin Square ◽  
Neutral Detergent Fiber ◽  
Starch Digestibility ◽  
Ruminal Fermentation ◽  
Beef Heifers ◽  
Passage Rate ◽  
Ruminal Ph ◽  
Pisum Sativum L

Abstract The objective of this study was to evaluate the stage of maturity at harvest for pea hay (Pisum sativum L., c.v. CDC Horizon) on dry matter intake (DMI), eating behavior, ruminal fermentation, and digestibility when fed to beef heifers. Pea hay was cut at EARLY (defined to occur when flat pods were on one or more nodes), MID (when seeds filled the pods at one or more nodes and the leaves were changing from green to gold), and LATE (yellow dry seeds filled pods on most or all of the nodes and the pods and leaves had a yellow color) phases, and was cured in the field and baled. Six ruminally-cannulated Speckle Park heifers were used in a replicated 3 × 3 Latin square design with three 18-d periods including 12 d for adaptation, 2 d for measurement of ruminal pool sizes, and 4 d for the collection of eating behavior, ruminal pH, ruminal digesta, and feces. For all treatments, the respective pea hay was included at 40% of the dietary DM. Stage of maturity at harvest for pea hay did not affect total DMI, pea hay DMI, or the total short-chain fatty acid concentration in ruminal fluid with averages of 8.6 kg/d, 3.2 kg/d, and 96.55 mM, respectively. The duration of time spent ruminating decreased with advancing pea hay maturity when reported as min/d, min/kg DMI, and min/kg neutral detergent fiber (NDF) (P ≤ 0.01). Mean ruminal pH also decreased with advancing pea maturity (P &lt; 0.01). The ruminal DM and undigested NDF corrected for OM pools were not affected by stage of maturity (P ≥ 0.55) nor was the rate of digestion for NDF. However, NDF passage rate decreased by 0.21%/h with advancing pea hay maturity (P = 0.02). Apparent total tract digestibility of NDF (average = 16.30%, P = 0.41) was not affected, but starch digestibility decreased from 96.10% to 93.08% with advancing pea hay maturity (P = 0.07). Overall, stage of maturity at harvest for pea hay does not appear to affect DMI or NDF digestibililty but decreases chewing activity, apparent total tract starch digestibility, ruminal pH, and ruminal NDF passage rate.

The effect of energy or protein supplementation on embryo survival rate in ewes

1995 ◽  
Vol 1995 ◽  
pp. 55-55
Author(s):  
M.G. Diskin ◽  
J.P. Hanrahan
Keyword(s):  
Survival Rate ◽  
Litter Size ◽  
Protein Supplementation ◽  
Ovulation Rate ◽  
Reproductive Efficiency ◽  
Protein Supplement ◽  
Embryo Survival ◽  
Rumen Undegradable Protein

Embryo survival rate is a major factor determining litter size and overall reproductive efficiency in sheep. In sheep, as ovulation rate increases the proportion of embryos surviving decreases. There is evidence, from cattle, that feeding a rumen-undegradable protein supplement enhances embryo survival rate. The objective of this experiment was to determine the effect on embryo survival rate in high prolific ewes of feeding an energy or protein supplement.

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