scholarly journals A Simulation Analysis of an Influenza Vaccine Production Plant in Areas of High Humanitarian Flow. A Preliminary Study for the Region of Norte de Santander (Colombia)

2021 ◽  
Vol 12 (1) ◽  
pp. 183
Author(s):  
Jefferson E. Contreras-Ropero ◽  
Silvia L. Ruiz-Roa ◽  
Janet B. García-Martínez ◽  
Néstor A. Urbina-Suarez ◽  
Germán L. López-Barrera ◽  
...  
Keyword(s):  
Simulation Analysis ◽  
Mdck Cells ◽  
Animal Cell ◽  
Scale Up ◽  
Raw Material ◽  
Production Plant ◽  
Biological Origin ◽  
Working Volume ◽  
Competitive Prices ◽  
Inoculum Volume

The production of vaccines of biological origin presents a tremendous challenge for researchers. In this context, animal cell cultures are an excellent alternative for the isolation and production of biologicals against several viruses, since they have an affinity with viruses and a great capacity for their replicability. Different variables have been studied to know the system’s ideal parameters, allowing it to obtain profitable and competitive products. Consequently, this work focuses its efforts on evaluating an alternative for producing an anti-influenza biological from MDCK cells using SuperPro Designer v8.0 software. The process uses the DMEN culture medium supplemented with nutrients as raw material for cell development; the MDCK cells were obtained from a potential scale-up with a final working volume of 500 L, four days of residence time, inoculum volume of 10%, and continuous working mode with up to a total of 7400 h/Yr of work. The scheme has the necessary equipment for the vaccine’s production, infection, and manufacture with yields of up to 416,698 units/h. In addition, it was estimated to be economically viable to produce recombinant vaccines with competitive prices of up to 0.31 USD/unit.

scholarly journals A Simulation Analysis of an Influenza Vaccine Production Plant in Areas of High Humanitarian Flow. A Case Study for the Region of Norte de Santander (Colombia)

2021 ◽  
Author(s):  
Jefferson Eduardo Contreras-Ropero ◽  
Silvia Liliana Ruiz-Roa ◽  
Janet B. García-Martínez ◽  
Néstor A. Urbina-Suarez ◽  
Germán L. López-Barrera ◽  
...  
Keyword(s):  
Simulation Analysis ◽  
Mdck Cells ◽  
Animal Cell ◽  
Scale Up ◽  
Raw Material ◽  
Production Plant ◽  
Biological Origin ◽  
Working Volume ◽  
Competitive Prices ◽  
Inoculum Volume

The production of vaccines of biological origin presents a tremendous challenge for researchers. In this context, animal cell cultures are an excellent alternative for the isolation and production of biologicals against several viruses since they have an affinity with viruses and a great capacity for their replicability. Different variables have been studied to know the system's ideal parameters, allowing it to obtain profitable and competitive products. Consequently, this work focuses its efforts on evaluating an alternative for producing an anti-influenza biological from MDCK cells using SuperPro Designer v8.0 software. The process uses the DMEN culture medium supplemented with nutrients as raw material for cell development; the MDCK cells were obtained from a potential scale-up with a final working volume of 500 L, four days of residence time, inoculum volume of 10%, and continuous working mode with up to a total of 7400 h/Yr of work. The scheme has the necessary equipment for the vaccine's production, infection, and manufacture with yields of up to 416,698 units/h. In addition, it was estimated to be economically viable to produce recombinant vaccines with competitive prices of up to 0.31 USD/unit.

scholarly journals Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particles

BMC Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Marc D. Hein ◽  
Prerna Arora ◽  
Pavel Marichal-Gallardo ◽  
Michael Winkler ◽  
Yvonne Genzel ◽  
...  
Keyword(s):  
Cell Culture ◽  
Influenza Virus ◽  
Influenza A ◽  
Mdck Cells ◽  
Animal Cell ◽  
Lethal Dose ◽  
High Morbidity ◽  
Stirred Tank Bioreactor ◽  
Working Volume ◽  
The Impact

Abstract Background Infections with influenza A virus (IAV) cause high morbidity and mortality in humans. Additional to vaccination, antiviral drugs are a treatment option. Besides FDA-approved drugs such as oseltamivir or zanamivir, virus-derived defective interfering (DI) particles (DIPs) are considered promising new agents. IAV DIPs typically contain a large internal deletion in one of their eight genomic viral RNA (vRNA) segments. Consequently, DIPs miss the genetic information necessary for replication and can usually only propagate by co-infection with infectious standard virus (STV), compensating for their defect. In such a co-infection scenario, DIPs interfere with and suppress STV replication, which constitutes their antiviral potential. Results In the present study, we generated a genetically engineered MDCK suspension cell line for production of a purely clonal DIP preparation that has a large deletion in its segment 1 (DI244) and is not contaminated with infectious STV as egg-derived material. First, the impact of the multiplicity of DIP (MODIP) per cell on DI244 yield was investigated in batch cultivations in shake flasks. Here, the highest interfering efficacy was observed for material produced at a MODIP of 1E−2 using an in vitro interference assay. Results of RT-PCR suggested that DI244 material produced was hardly contaminated with other defective particles. Next, the process was successfully transferred to a stirred tank bioreactor (500 mL working volume) with a yield of 6.0E+8 PFU/mL determined in genetically modified adherent MDCK cells. The produced material was purified and concentrated about 40-fold by membrane-based steric exclusion chromatography (SXC). The DI244 yield was 92.3% with a host cell DNA clearance of 97.1% (99.95% with nuclease digestion prior to SXC) and a total protein reduction of 97.2%. Finally, the DIP material was tested in animal experiments in D2(B6).A2G-Mx1r/r mice. Mice infected with a lethal dose of IAV and treated with DIP material showed a reduced body weight loss and all animals survived. Conclusion In summary, experiments not only demonstrated that purely clonal influenza virus DIP preparations can be obtained with high titers from animal cell cultures but confirmed the potential of cell culture-derived DIPs as an antiviral agent.

scholarly journals Scaling Up Delivery of Biofortified Staple Food Crops Globally: Paths to Nourishing Millions

2021 ◽  
pp. 037957212098250
Author(s):  
Jennifer K. Foley ◽  
Kristina D. Michaux ◽  
Bho Mudyahoto ◽  
Laira Kyazike ◽  
Binu Cherian ◽  
...  
Keyword(s):  
Pearl Millet ◽  
Large Scale ◽  
Scale Up ◽  
Lessons Learned ◽  
Raw Material ◽  
Micronutrient Deficiencies ◽  
Seed Systems ◽  
Public And Private ◽  
Context Specific ◽  
Public And Private Sector

Background: Micronutrient deficiencies affect over one quarter of the world’s population. Biofortification is an evidence-based nutrition strategy that addresses some of the most common and preventable global micronutrient gaps and can help improve the health of millions of people. Since 2013, HarvestPlus and a consortium of collaborators have made impressive progress in the enrichment of staple crops with essential micronutrients through conventional plant breeding. Objective: To review and highlight lessons learned from multiple large-scale delivery strategies used by HarvestPlus to scale up biofortification across different country and crop contexts. Results: India has strong public and private sector pearl millet breeding programs and a robust commercial seed sector. To scale-up pearl millet, HarvestPlus established partnerships with public and private seed companies, which facilitated the rapid commercialization of products and engagement of farmers in delivery activities. In Nigeria, HarvestPlus stimulated the initial acceptance and popularization of vitamin A cassava using a host of creative approaches, including “crowding in” delivery partners, innovative promotional programs, and development of intermediate raw material for industry and novel food products. In Uganda, orange sweet potato (OSP) is a traditional subsistence crop. Due to this, and the lack of formal seed systems and markets, HarvestPlus established a network of partnerships with community-based nongovernmental organizations and vine multipliers to popularize and scale-up delivery of OSP. Conclusions: Impact of biofortification ultimately depends on the development of sustainable markets for biofortified seeds and products. Results illustrate the need for context-specific, innovative solutions to promote widespread adoption.

scholarly journals Mechanical Properties of Alkali Activated Material Based on Red Clay and Silica Gel Precursor

2021 ◽  
Vol 25 (1) ◽  
pp. 931-943
Author(s):  
Girts Bumanis ◽  
Danute Vaiciukyniene
Keyword(s):  
Silica Gel ◽  
Raw Materials ◽  
Raw Material ◽  
Alkali Activation ◽  
Production Plant ◽  
X Ray Diffraction ◽  
Red Clay ◽  
Significant Strength ◽  
Naoh Solution ◽  
Alkali Activated

Abstract The search for alternative alumosilicates source for production of alkali activated materials (AAM) is intensively researched. Wide spread of natural materials such as clays and waste materials are one of potential alternatives. In this research AAM was made from local waste brick made of red clay and calcined low-carbonate illite clay precursor and its properties evaluated. Waste silica gel containing amorphous silica from fertilizer production plant was proposed as additional raw material. 6 M and 7 M NaOH alkali activation solutions were used to obtain AAM. Raw materials were characterized by X-ray diffraction, laser particle size analyser, DTA/TG. Raw illite clay was calcined at a temperature of 700 to 800 °C. Waste brick was ground similar as raw clay and powder was obtained. Replacement of red clay with silica gel from 2–50 wt.% in mixture composition was evaluated. Results indicate that the most effective activator was 6 M NaOH solution and AAM with strength up to 13 MPa was obtained. Ground brick had the highest strength results and compressive strength of AAM reached 25 MPa. Silica gel in small quantities had little effect of AAM strength while significant strength reduction was observed with the increase silica gel content. The efflorescence was observed for samples with silica gel.

scholarly journals WEEE collection and CRM recovery trials: piloting a holistic approach for Scotland

2018 ◽  
Vol 20 (4) ◽  
pp. 712-718
Keyword(s):  
Raw Materials ◽  
Scale Up ◽  
Holistic Approach ◽  
Extraction Methods ◽  
Social Enterprises ◽  
Raw Material ◽  
Household Waste ◽  
Sustainable Supply Chain ◽  
Gold Silver ◽  
Critical Raw Materials

<p>Re-Tek UK and its partners, Enscape Consulting and the University of West of Scotland commenced trials for the collection and recovery of critical raw materials from waste electrical and electronic (WEEE) products in July 2016. Sponsored by the EU LIFE funded project ‘Critical Raw Material Closed Loop Recovery’ coordinated by WRAP with EARN, ERP UK Ltd, KTN Ltd and Wuppertal Institute as beneficiaries. The trials are aimed at boosting the recovery of critical raw materials (CRMs) from household waste electrical and electronic products (WEEE) and Information Communications Technology (ICT) in particular, after functioning equipment is separated out for re-use. The new collection models provided residents with the opportunity to drop-off unwanted electrical and electronic appliances at a time and place that suits them, through a collaborative approach which encourages local authorities, educational establishments, businesses, and Social Enterprises, etc to act as hub sites. Hubs were designed to minimize product damage and encourage drop-off, rather than hoarding. Extraction methods developed after the collection phase of the trial looked at the opportunity to recover cobalt, gold and silver from ICT products, with the potential to inform how a more sustainable supply chain could be developed in Scotland. The elements studied were selected to demonstrate financial opportunity (gold/silver) and a strategic priority material (cobalt) for long term supply. These are based on bioleaching and electrochemical recovery using novel carbon based electrode systems, and chemical processing methods using extraction techniques with an assessment of pilot performance and scale up challenges. Our report is on the state of progress towards practical solutions to WEEE and CRM recovery.</p>

scholarly journals Removal of orthophosphates in water by modified carbonate material of biological origin

2017 ◽  
Author(s):  
Marco Tadeu Gomes Vianna ◽  
Marcia Marques
Keyword(s):  
Exotic Species ◽  
Removal Efficiency ◽  
Low Cost ◽  
Industrial Effluents ◽  
Water Bodies ◽  
Raw Material ◽  
Biological Origin ◽  
Chemically Modified ◽  
Removal Capacity ◽  
P Removal

The excessive release of phosphorus (P) by discharge of domestic and industrial effluents is directly associated with the eutrophication of water bodies. Therefore, an efficient removal of P from effluents is required. The method most commonly used for P removal from wastewater is chemical precipitation. However, this technique is relatively expensive and demands a proper disposal for the sludge. Therefore, the development of new materials with low cost but high P removal efficiency has been investigated. The introduction of exotic species in aquatic environments is considered a serious environmental problem in different parts of the world. Considering that, many of these species have high concentrations of carbonates in their exoskeleton composition, which is potentially useful in water treatment, particularly for P removal the use of such material as adsorbent has been tested. The present study aimed to investigate the capacity of the exoskeleton of exotic species in powder form to remove orthophosphates from water comparing the raw material (RCS), with physically modified (CSA) and chemically modified (CSC) material. To study the orthophosphates removal efficiency, a factorial design with central composite rotational design (CCRD) was applied. In order to optimize the P removal, the influence of the independent variables adsorbent/adsorbate ratio, pH and temperature was investigated with the kinetic control associate at each configuration obtained by CCRD. The P removal capacity of RCS varied from 125.0 mgP kg-1 to 1002.5 mgP kg-1; the removal capacity of CSA varied from 237.5 mgP kg-1 to 1540.0 mgP kg-1. The removal capacity of CSC varied from 5212.5 mgP kg-1 to 12672.5 mgP kg-1. Based on the preliminary results, the exoskeleton powder showed to be a potentially sustainable alternative as adsorbent material (mostly the chemically modified form CSC) useful in several applications, such as the treatment of urban and industrial wastewaters to prevent eutrophication of water bodies and population control of exotic species due to the commercial exploitation. 

Novel scale-up strategy based on three-dimensional shear space for animal cell culture

2020 ◽  
Vol 212 ◽  
pp. 115329 ◽  
Author(s):  
Chao Li ◽  
Xiaonuo Teng ◽  
Huadong Peng ◽  
Xiaoping Yi ◽  
Yingping Zhuang ◽  
...  
Keyword(s):  
Cell Culture ◽  
Animal Cell ◽  
Scale Up ◽  
Three Dimensional ◽  
Animal Cell Culture

scholarly journals Design, Cost Estimation and Sensitivity Analysis for a Production Process of Activated Carbon from Waste Nutshells by Physical Activation

Processes ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 945
Author(s):  
Marcelo León ◽  
Javier Silva ◽  
Samuel Carrasco ◽  
Nelson Barrientos
Keyword(s):  
Sensitivity Analysis ◽  
Activated Carbon ◽  
Cost Estimation ◽  
Net Present Value ◽  
Cash Flows ◽  
Raw Material ◽  
Total Annual Cost ◽  
Physical Activation ◽  
Production Plant ◽  
Tax Rate

A conceptual design of an industrial production plant for activated carbon was developed to process 31.25 tons/day of industrial waste nutshells as the raw material and produce 6.6 ton/day of activated carbon using steam as an activation agent. The design considered the cost of the main equipment, the purchase price of the nutshells, basic services, and operation. A sensitivity analysis was developed, considering the price of the finished product and the volume of raw material processing varied up to ±25%. Furthermore, the total annual cost of the product was determined based on the production of 2100 tons/year of activated carbon. Two cash flows were developed and projected to periods of 10 years and 15 years of production, using a tax rate of 27%, a low discount rate (LDR) of 10% per year, and without external financing. For a 10-year production project, the net present value (NPV) was USD 2,785,624, the internal return rate (IRR) 21%, the return on investment (ROI) 25%, and the discounted payback period (DPP) after the fifth year. Considering a project with 15 years of production, the NPV was USD 4,519,482, the IRR at 23%, the ROI 24%, and the DPP after the fifth year of production.

scholarly journals Synthesis of DEAE-Soybean Starch Microspheres for Adhere Animal Cell Culture

2017 ◽  
Vol 9 (8) ◽  
pp. 91
Author(s):  
Lei Zhang ◽  
Mingsheng Li ◽  
Zhongren Ma ◽  
Yuping Feng
Keyword(s):  
Cell Culture ◽  
Mdck Cells ◽  
Animal Cell ◽  
Low Cost ◽  
Animal Cell Culture ◽  
Cross Linking ◽  
Animal Cells ◽  
Inoculation Density ◽  
Animal Cell Cultures ◽  
Adhere Cell

The present study outlines the synthesis of a new microcarrier for anchorage-dependent animal cell cultures. The new microcarriers were synthesized from the cross-linking soybean starch microspheres followed by modification with 2-diethylaminoethyl (DEAE). Furthermore, 5 g/100 mL of wet microspheres DEAE-soybean starch microspheres were applied in the adhere cell culture, with an inoculation density 2.0 × 105 cells/mL of BHK-21, Marc-145, and MDCK cells. The cells were shown to grow well in the DEAE-soybean starch microcarrier, with BHK-21 cells showing a higher cell density after 144 h (2.5 × 106 cells/mL) compared to cells grown on the commercial product Cytodex 1 (2.2 × 106 cells/mL). These starch microcarriers have a potential application in anchorage-dependent animal cells culture, due to its low cost and its simple process.

Company News

2001 ◽  
Vol 05 (21) ◽  
pp. 531-546
Keyword(s):  
Joint Venture ◽  
Ceramic Membrane ◽  
Membrane Filter ◽  
Scale Up ◽  
Rocky Mountain ◽  
Cancer Drug ◽  
Production Plant ◽  
Joint Research ◽  
Aids Vaccine ◽  
Set Up

US-based Alara Expands MetriScan™ Distribution into China. Varsal Sets Up Special Chemical Facility in China. Three Taiwan-based Biotech Firms to Jointly Set Up Vaccine Production Plant. Malaysia's Sirim Comes Up with Latest Ceramic Membrane Filter Technology. Fuzhou Branch of Rocky Mountain Launches New Product. SurroMed Expands Global Efforts with New Singapore Facility. Malaysia's Top Glove Expands into International Market. TNT Offers Bio-logistics Services in Singapore. Malaysia's ITAV Enters into Agreement with Eastgate and Biotech Asia. Thailand to Undertake Bio-diesel Fuel Project. Chugai Sells Business Rights of Medical Device Products to Kobayashi. Sumitomo in Joint Research with RIKEN on Pharmaceutical Development Based on Genome Information. Settlement of Investigation into Takeda Subsidiary's Marketing Practices of Prostate Cancer Drug. Novartis and Korea Yet to Agree on Leukemia Drug Price. VaxGen Receives Funding for Development of AIDS Vaccine in India, China and Africa. Fujisawa Launches Protopic Ointment in Canada. BresaGen and Image Guided Neurologics to Produce and Distribute Proprietary Cell Delivery Device. Progen Industries and BresaGen Sign Manufacturing Agreement. India's Kopran Gets Better Deal for Heart Drug Aten. Reliance Life Sciences to Scale Up Biotech. NZ's Delphic and Japan's Sysmex Announce Joint Venture. Australian Biotech Companies Get Another A$3.6 Million of Funding.

Sign in / Sign up

Export Citation Format

Share Document