scholarly journals A Review on Haematococcus pluvialis Bioprocess Optimization of Green and Red Stage Culture Conditions for the Production of Natural Astaxanthin

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 256 ◽  
Author(s):  
Siti Nur Hazwani Oslan ◽  
Noor Fazliani Shoparwe ◽  
Abdul Hafidz Yusoff ◽  
Ainihayati Abdul Rahim ◽  
Chang Shen Chang ◽  
...  
Keyword(s):  
Haematococcus Pluvialis ◽  
Carbon Sources ◽  
Biomass Accumulation ◽  
Culture Conditions ◽  
Bioprocess Optimization ◽  
Cellular Processes ◽  
Green Microalga ◽  
Secondary Carotenoid ◽  
Stage Process ◽  
Green Stage

As the most recognizable natural secondary carotenoid astaxanthin producer, the green microalga Haematococcus pluvialis cultivation is performed via a two-stage process. The first is dedicated to biomass accumulation under growth-favoring conditions (green stage), and the second stage is for astaxanthin evolution under various stress conditions (red stage). This mini-review discusses the further improvement made on astaxanthin production by providing an overview of recent works on H. pluvialis, including the valuable ideas for bioprocess optimization on cell growth, and the current stress-exerting strategies for astaxanthin pigment production. The effects of nutrient constituents, especially nitrogen and carbon sources, and illumination intensity are emphasized during the green stage. On the other hand, the significance of the nitrogen depletion strategy and other exogenous factors comprising salinity, illumination, and temperature are considered for the astaxanthin inducement during the red stage. In short, any factor that interferes with the cellular processes that limit the growth or photosynthesis in the green stage could trigger the encystment process and astaxanthin formation during the red stage. This review provides an insight regarding the parameters involved in bioprocess optimization for high-value astaxanthin biosynthesis from H. pluvialis.

scholarly journals Mixotrophic Growth of Astaxanthin-Rich Alga Haematococcus pluvialis using Refined Crude Glycerol as Carbon Substrate: Batch and Fed-Batch Cultivations

2021 ◽  
Vol 18 (2) ◽  
Author(s):  
Pongsathorn DECHATIWONGSE ◽  
Wanna CHOORIT
Keyword(s):  
Crude Glycerol ◽  
Haematococcus Pluvialis ◽  
Exponential Growth Phase ◽  
Carbon Substrate ◽  
Fed Batch ◽  
Bg11 Medium ◽  
Green Microalga ◽  
Green Stage ◽  
A Current ◽  
Mixotrophic Conditions

Due to a current overabundance of crude glycerol produced from the biodiesel industry, the compound has the potential to be used as an inexpensive carbon source for growing the green microalga Haematococcus pluvialis (H. pluvialis), the richest source of natural astaxanthin (ATX). In order to investigate the practical use of crude glycerol, microalgal cultures were grown mixotrophically and heterotrophically in BG11 medium with the supplementation of refined crude glycerol, a mixture of glycerol and ethanol, under 2, 5 and 7 g L-1, using photoautotrophic cultivation as a control. H. pluvialis green-stage growth and red-stage ATX accumulation were effectively facilitated by mixotrophic conditions, with the highest µ of 0.27 ± 0.03 day-1 and the highest ATX content of (3.5 ± 0.4 % wt) (both observed under 7 g glycerol L-1). In contrast, growth was completely inhibited under heterotrophic conditions. Under repeated fed-batch operation, the exponential growth phase, during green-stage mixotrophic cultivation, was significantly extended from 5 days (in batch) to 24 days, making biomass yield of 1.86 ± 0.06 g DCW L-1 (around 2.6-fold higher). Monitoring of substrates (glycerol, ethanol and nitrate) in the broth was carried out and subsequently suggested that further optimization of media could be made.

Influência do CO2 no Crescimento de Haematococcus Pluvialis e na Produção de Carotenoides

UNICIÊNCIAS ◽  
2019 ◽  
Vol 22 (3Esp) ◽  
pp. 25
Author(s):  
Daiane Felix Reis ◽  
Francisco Roberto da Silva Machado Junior ◽  
Joana Da Costa Ores ◽  
Ailton Cesar Lemes ◽  
Carlos Andre Veiga Burkert ◽  
...  
Keyword(s):  
Cell Growth ◽  
Haematococcus Pluvialis ◽  
Carbon Sources ◽  
Nutrient Deficiency ◽  
Basal Medium ◽  
Culture Conditions ◽  
Cellular Growth ◽  
Co2 Injection ◽  
Total Carotenoids ◽  
Freshwater Microalgae

O crescimento celular da microalga de água doce Haematococcus pluvialis e a bioprodução de carotenoides são influenciados pelas diferentes condições de cultivo, como deficiência de nutrientes, iluminância, aeração, agitação, temperatura e pH, alterando sua morfologia celular e produzindo cistos avermelhados (carotenogênese). A aeração nos cultivos de microalgas está relacionada a alguns fatores que influenciam no crescimento celular. As microalgas absorvem e utilizam CO2 como a principal fonte de carbono no crescimento celular. Logo, a biossíntese de pigmentos pode ocorrer pela limitação do nitrogênio em presença de excesso de fontes de carbono. O objetivo desse trabalho foi investigar a influência do emprego de CO2 na aeração do cultivo da microalga Haematococcus pluvialis sob o crescimento celular e a bioprodução de carotenoides. No cultivo foi utilizado o meio mixotrófico BBM (Bold Basal Medium) e acetato de sódio, empregando 20% de inóculo em pH inicial de 7,0, aeração de 0,30 L.min-1, com 30% de injeção de CO2 uma vez ao dia durante 1 h, sob iluminância de 6 klux, à 25 ºC durante 22 dias. Nestas condições o crescimento celular alcançou o máximo de 1,13±0,39 g.L-1 (10 dias) e os carotenoides totais 2949,91±988,65 µg.g-1, onde foi observado que a suplementação de CO2 como fonte de carbono dissolvida no meio de cultivo pode influenciar o crescimento celular e os carotenoides totais. Palavras-chave: Microalga. Pigmento. Aeração. Cultivo. AbstractThe cellular growth of the freshwater microalgae Haematococcus pluvialis and the bioproduction of carotenoids are influenced by the different culture conditions, such as nutrient deficiency, illuminance, aeration, agitation, temperature and pH, altering its cellular morphology and producing reddish cysts (carotenogenesis). Aeration in microalgae cultures is related to some factors that influence cell growth. Microalgae absorb and utilize CO2 as the main source of carbon in cell growth. Therefore, the biosynthesis of pigments can occur by the limitation of nitrogen in the presence of excess carbon sources. The objective of this work was to investigate the influence of the use of CO2 on the aeration of the microalgae Haematococcus pluvialis under cell growth and bioproduction of carotenoids. In the culture, mixotrophic medium BBM (Bold Basal Medium) and sodium acetate were used, using 20% of inoculum at initial pH of 7.0, aeration of 0.30 L.min-1, with 30% of CO2 injection once a day for 1 h under 6 Klux illuminance at 25 ° C for 22 days. Under these conditions the cell growth reached a maximum of 1.13 ± 0.39 g. L-1 (10 days) and the total carotenoids 2949.91 ± 988.65 μg.g-1, where it was observed that CO2 supplementation as a source of carbon dissolved in the culture medium may influence cell growth and total carotenoids. Keywords: microalgae; pigment; aeration; cultivation. 

scholarly journals Growth of Haematococcus pluvialis on a Small-Scale Angled Porous Substrate Photobioreactor for Green Stage Biomass

2021 ◽  
Vol 11 (4) ◽  
pp. 1788
Author(s):  
Thanh-Tri Do ◽  
Binh-Nguyen Ong ◽  
Tuan-Loc Le ◽  
Thanh-Cong Nguyen ◽  
Bich-Huy Tran-Thi ◽  
...  
Keyword(s):  
Light Intensity ◽  
Algal Biomass ◽  
Haematococcus Pluvialis ◽  
Biomass Productivity ◽  
Pilot Scale ◽  
Small Scale ◽  
Porous Substrate ◽  
Low Light ◽  
Green Algal ◽  
Green Stage

In the production of astaxanthin from Haematococcus pluvialis, the process of growing algal biomass in the vegetative green stage is an indispensable step in both suspended and immobilized cultivations. The green algal biomass is usually cultured in a suspension under a low light intensity. However, for astaxanthin accumulation, the microalgae need to be centrifuged and transferred to a new medium or culture system, a significant difficulty when upscaling astaxanthin production. In this research, a small-scale angled twin-layer porous substrate photobioreactor (TL-PSBR) was used to cultivate green stage biomass of H. pluvialis. Under low light intensities of 20–80 µmol photons m−2·s−1, algae in the biofilm consisted exclusively of non-motile vegetative cells (green palmella cells) after ten days of culturing. The optimal initial biomass density was 6.5 g·m−2, and the dry biomass productivity at a light intensity of 80 µmol photons m−2·s−1 was 6.5 g·m−2·d−1. The green stage biomass of H. pluvialis created in this small-scale angled TL-PSBR can be easily harvested and directly used as the source of material for the inoculation of a pilot-scale TL-PSBR for the production of astaxanthin.

scholarly journals Combined Production of Astaxanthin and β-Carotene in a New Strain of the Microalga Bracteacoccus aggregatus BM5/15 (IPPAS C-2045) Cultivated in Photobioreactor

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 643
Author(s):  
Konstantin Chekanov ◽  
Daniil Litvinov ◽  
Tatiana Fedorenko ◽  
Olga Chivkunova ◽  
Elena Lobakova
Keyword(s):  
Bubble Column ◽  
Biomass Accumulation ◽  
Natural Antioxidants ◽  
Gas Chromatography Mass Spectrometry ◽  
Stage Scheme ◽  
Green Microalga ◽  
Animal Feeding ◽  
Carotenoid Synthesis ◽  
Bubble Column Photobioreactor ◽  
Β Carotene

Carotenoids astaxanthin and β-carotene are widely used natural antioxidants. They are key components of functional food, cosmetics, drugs and animal feeding. They hold leader positions on the world carotenoid market. In current work, we characterize the new strain of the green microalga Bracteacoccus aggregatus BM5/15 and propose the method of its culturing in a bubble-column photobioreactor for simultaneous production of astaxanthin and β-carotene. Culture was monitored by light microscopy and pigment kinetics. Fatty acid profile was evaluated by tandem gas-chromatography–mass spectrometry. Pigments were obtained by the classical two-stage scheme of autotrophic cultivation. At the first, vegetative, stage biomass accumulation occurred. Maximum specific growth rate and culture productivity at this stage were 100–200 mg∙L−1∙day−1, and 0.33 day−1, respectively. At the second, inductive, stage carotenoid synthesis was promoted. Maximal carotenoid fraction in the biomass was 2.2–2.4%. Based on chromatography data, astaxanthin and β-carotene constituted 48 and 13% of total carotenoid mass, respectively. Possible pathways of astaxanthin synthesis are proposed based on carotenoid composition. Collectively, a new strain B. aggregatus BM5/15 is a potential biotechnological source of two natural antioxidants, astaxanthin and β-carotene. The results give the rise for further works on optimization of B. aggregatus cultivation on an industrial scale.

scholarly journals Phyllobacterium loti sp. nov. isolated from nodules of Lotus corniculatus

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 781-786 ◽  
Author(s):  
Maximo Sánchez ◽  
Martha-Helena Ramírez-Bahena ◽  
Alvaro Peix ◽  
María J. Lorite ◽  
Juan Sanjuán ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Carbon Sources ◽  
Lotus Corniculatus ◽  
Culture Conditions ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
The 16S Rrna Gene

Strain S658T was isolated from a Lotus corniculatus nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and atpD gene showed that this strain clustered within the genus Phyllobacterium . The closest related species was, in both cases, Phyllobacterium trifolii PETP02T with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the atpD gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658T differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in Lotus corniculatus. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus Phyllobacterium , for which the name Phyllobacterium loti sp. nov. is proposed. The type strain is S658T( = LMG 27289T = CECT 8230T).

scholarly journals Identification and Content of Astaxanthin and Its Esters from Microalgae Haematococcus pluvialis by HPLC-DAD and LC-QTOF-MS after Extraction with Various Solvents

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2413
Author(s):  
Biljana Todorović ◽  
Veno Jaša Grujić ◽  
Andreja Urbanek Krajnc ◽  
Roman Kranvogl ◽  
Jana Ambrožič-Dolinšek
Keyword(s):  
High Performance ◽  
Haematococcus Pluvialis ◽  
Methyl Tert Butyl Ether ◽  
Diode Array ◽  
Diode Array Detection ◽  
Butyl Ether ◽  
Flight Mass Spectrometry ◽  
Green Microalga ◽  
Array Detection ◽  
Long Chain

Haematococcus pluvialis, a unicellular green microalga that produces a secondary metabolite under stress conditions, bears one of the most potent antioxidants, namely xanthophyll astaxanthin. The aim of our study was to determine the content of astaxanthin and its esterified forms using three different solvents—methyl tert-butyl ether (MTBE), hexane isopropanol (HEX -IPA) and acetone (ACE)—and to identify them by using high performance liquid chromatography coupled with diode array detection and the quadrupole time-of-flight mass spectrometry (HPLC-DAD and LC-QTOF-MS) technique. We identified eleven astaxanthin monoesters, which accounted for 78.8% of the total astaxanthin pool, six astaxanthin diesters (20.5% of total), while free astaxanthin represented the smallest fraction (0.7%). Astaxanthin monoesters (C16:2, C16:1, C16:0), which were the major bioactive compounds in the H. pluvialis samples studied, ranged from 10.2 to 11.8 mg g−1 DW. Astaxanthin diesters (C18:4/C18:3, C18:1/C18:3) were detected in the range between 2.3 and 2.6 mg g−1 DW. All three solvents were found to be effective for extraction, but MTBE and hexane-isopropanol extracted the greatest amount of free bioactive astaxanthin. Furthermore, MTBE extracted more low-chain astaxanthin monoesters (C16), and hexane-isopropanol extracted more long-chain monoesters (C18 and above) and more diesters. We can conclude that MTBE is the solvent of choice for the extraction of monoesters and hexane-isopropanol for diesters.

scholarly journals Effect of nitrate concentration on growth of green microalga Haematococcus pluvialis under laboratory conditions

2013 ◽  
Vol 35 (2) ◽  
Author(s):  
Le Thi Thom ◽  
Luu Thi Tam ◽  
Dinh Ngoc Mai ◽  
Hoang Thi Lan Anh ◽  
Ngo Thi Hoai Thu ◽  
...  
Keyword(s):  
Haematococcus Pluvialis ◽  
Nitrate Concentration ◽  
Laboratory Conditions ◽  
Green Microalga

scholarly journals Putative metabolic pathway for the bioproduction of bikaverin and intermediates thereof in the wild Fusarium oxysporum LCP531 strain

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Juliana Lebeau ◽  
Thomas Petit ◽  
Laurent Dufossé ◽  
Yanis Caro
Keyword(s):  
Metabolic Pathway ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Pharmaceutical Products ◽  
Culture Conditions ◽  
Fusarium Fujikuroi ◽  
Submerged Cultures ◽  
In The Wild ◽  
Considerable Work ◽  
Pharmaceutical Uses

AbstractFungal naphthoquinones, like red bikaverin, are of interest due to their growing applications in designing pharmaceutical products. Though considerable work has been done on the elucidation of bikaverin biosynthesis pathway in Fusarium fujikuroi, very few reports are available regarding its bioproduction in F. oxysporum. We are hereby proposing a putative metabolic pathway for bikaverin bioproduction in a wild F. oxysporum strain by cross-linking the pigment profiles we obtained under two different fermentation conditions with literature. Naphthoquinone pigments were extracted with a pressurized liquid extraction method, and characterized by HPLC–DAD and UHPLC-HRMS. The results led to the conclusions that the F. oxysporum LCP531 strain was able to produce bikaverin and its various intermediates, e.g., pre-bikaverin, oxo-pre-bikaverin, dinor-bikaverin, me-oxo-pre-bikaverin, and nor-bikaverin, in submerged cultures in various proportions. To our knowledge, this is the first report of the isolation of these five bikaverin intermediates from F. oxysporum cultures, providing us with steady clues for confirming a bikaverin metabolic pathway as well as some of its regulatory patterns in the F. oxysporum LCP531 strain, based on the previously reported model in F. fujikuroi. Interestingly, norbikaverin accumulated along with bikaverin in mycelial cells when the strain grew on simple carbon and nitrogen sources and additional cofactors. Along bikaverin production, we were able to describe the excretion of the toxin beauvericin as main extrolite exclusively in liquid medium containing complex nitrogen and carbon sources, as well as the isolation of ergosterol derivate in mycelial extracts, which have potential for pharmaceutical uses. Therefore, culture conditions were also concluded to trigger some specific biosynthetic route favoring various metabolites of interest. Such observation is of great significance for selective production of pigments and/or prevention of occurrence of others (aka mycotoxins).

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