scholarly journals Silymarin-Enriched Biostimulant Foliar Application Minimizes the Toxicity of Cadmium in Maize by Suppressing Oxidative Stress and Elevating Antioxidant Gene Expression

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 465
Author(s):  
Hesham F. Alharby ◽  
Hassan S. Al-Zahrani ◽  
Khalid R. Hakeem ◽  
Hameed Alsamadany ◽  
El-Sayed M. Desoky ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Lipid Peroxidation ◽  
Antioxidative Enzymes ◽  
Irrigation Water ◽  
Foliar Spray ◽  
Cd Stress ◽  
Enzyme Gene ◽  
Maize Plants ◽  
Maize Grain

For maize, the potential preventive role of foliar spraying with an extract derived from maize grain (MEg, 2%), silymarin (Sm, 0.5 mM), or silymarin-enriched MEg (MEg-Sm) in attenuating the stress effects of cadmium (Cd, 0.5 mM) was examined using a completely randomized design layout. Under normal conditions, foliar spraying with MEg, Sm, or MEg-Sm was beneficial (with MEg-Sm preferred) for maize plants, whereas the benefit was more pronounced under Cd stress. The use of Cd through irrigation water decreased plant growth traits, photosynthetic efficiency, including instantaneous carboxylation efficiency, Fv/Fm, and pigment contents, and hormonal contents (e.g., auxin, gibberellins, cytokinins including trans-zeatin, and salicylic acid). These undesired findings were due to an increase in Cd content, leading to increased levels of oxidative stress (O2•− and H2O2), ionic leakage, and lipid peroxidation. Therefore, this damage resulted in an increase in the activities of nonenzymatic antioxidants, Sm, antioxidative enzymes, and enzyme gene expression. However, under Cd stress, although foliar spray with MEg or Sm had better findings than control, MEg-Sm had better findings than MEg or Sm. Application of MEg-Sm greatly increased photosynthesis efficiency, restored hormonal homeostasis, and further increased the activities of various antioxidants, Sm, antioxidative enzymes, and enzyme gene expression. These desired findings were due to the suppression of the Cd content, and thus the levels of O2•−, H2O2, ionic leakage, and lipid peroxidation, which were positively reflected in the growth and accumulation of dry matter in maize plants. The data obtained in this study recommend applying silymarin-enriched maize grain extract (MEg-Sm at 0.24 g Sm L−1 of MEg) as a spray solution to maize plants when exposed to excess Cd in soil or irrigation water.

Penconazole and calcium ameliorate drought stress in canola by upregulating the antioxidative enzymes

2020 ◽  
Vol 47 (9) ◽  
pp. 825 ◽  
Author(s):  
Maryam Rezayian ◽  
Vahid Niknam ◽  
Hassan Ebrahimzadeh
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Antioxidative Enzymes ◽  
Proline Content ◽  
Fresh Weight ◽  
Negative Effects ◽  
Mda Content ◽  
Effects Of Drought

The aim of this research was to gauge the alternations in the lipid peroxidation and antioxidative enzyme activity in two cultivars (cv. RGS003 and cv. Sarigol) of canola under drought stress and drought tolerance amelioration by penconazole (PEN) and calcium (Ca). Plants were treated with different polyethylene glycol (PEG) concentrations (0, 5, 10 and 15%) without or with PEN (15 mg L–1) and Ca (15 mM). The Ca treatment prevented the negative effects of drought on fresh weight (FW) in RGS003 and Sarigol at 5 and 15% PEG respectively. Ca and PEN/Ca treatments caused significant induction in the proline content in Sarigol at 15% PEG; the latter treatment was accompanied by higher glycine betaine (GB), lower malondialdehyde (MDA) and growth recovery. Hydrogen peroxide (HO2) content in Sarigol was proportional to the severity of drought stress and all PEN, Ca and PEN/Ca treatments significantly reduced the H2O2 content. PEN and PEN/Ca caused alleviation of the drought-induced oxidative stress in RGS003. RGS003 cultivar exhibited significantly higher antioxidative enzymes activity at most levels of drought, which could lead to its drought tolerance and lower MDA content. In contrast to that of Sarigol, the activity of catalase and superoxide dismutase (SOD) increased with Ca and PEN/Ca treatments in RGS003 under low stress. The application of PEN and Ca induced significantly P5CS and SOD expression in RGS003 under drought stress after 24 h. Overall, these data demonstrated that PEN and Ca have the ability to enhance the tolerance against the drought stress in canola plants.

scholarly journals Effects of cadmium-induced oxidative stress on growth and nitrogen assimilation in blackgram [Vigna mungo (L.) Hepper]

2013 ◽  
Vol 58 (1) ◽  
pp. 31-39
Author(s):  
Mohammad Mobin
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Nitrogen Assimilation ◽  
Dry Weight ◽  
Vigna Mungo ◽  
Cd Stress ◽  
Cd Accumulation ◽  
Shoot Dry Weight ◽  
Activity Of Enzymes ◽  
Consequent Increase

Cadmium (Cd) accumulation, oxidative damage, and nitrogen metabolism were studied in roots and leaves of 30-d-old blackgram plants [Vigna mungo (L.) Hepper], grown in a mixture of soil and compost (3:1) with different Cd concentrations. Significant reductions in both root and shoot dry weight were noted. The concentration of Cd in roots and leaves increased with increasing Cd levels. The level of lipid peroxidation elevated with a consequent increase in H2O2 content under Cd stress in both plant organs. The activity of enzymes mediating the nitrogen assimilation in roots and leaves was greatly reduced in the presence of Cd, except glutamate dehydrogenase (GDH) which showed a significant increase.

Changes In Antioxidant Systems And Sucrose Metabolism In Maize Varieties Exposed To Cd

2021 ◽  
Author(s):  
Cong Li ◽  
Yingdi Cao ◽  
Tianfeng Li ◽  
Meiyu Guo ◽  
Xinglin Ma ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Germination Rate ◽  
Sucrose Metabolism ◽  
Antioxidant Systems ◽  
Cd Stress ◽  
Physiological Mechanisms ◽  
Stress Response Genes ◽  
Maize Varieties ◽  
Tolerant Variety ◽  
Maize Plants

Abstract While it is generally accepted that different maize varieties respond in various ways to cadmium (Cd) stress, the physiological mechanisms that determine how they respond are not well-defined. We do know, however, that antioxidant systems and sucrose metabolism help plants to cope with abiotic stresses, including stress from Cd. Seed is sensitive to Cd stress during germination stage. In this study, we investigated how the antioxidant systems, sucrose metabolism, abscisic acid (ABA) and gibberellin (GA3) concentration in two maize varieties with low (FY9) or high (SY33) sensitivities to Cd changed when Cd was added at 20 mg L−1 over different germination stages (3, 6, and 9 days). As Cd accumulated, the germination rate decreased, and growth was inhibited. The O2•-, malondialdehyde (MDA), and proline (Pro) concentrations, and the superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and lipoxygenase (LOX) activities increased compared to the CK (without Cd). The expression levels of three genes (ZmOPR2, ZmOPR5 and ZmPP2C6) that respond to oxidative stress increased differently in two varieties under Cd stress. The activity of the antioxidant system including the transcript levels of oxidative stress response genes were higher in Cd-tolerant variety, FY9, than in sensitive variety, SY33. And then, we also examined sucrose metabolism levels that were increased compared to the CK. However, it was more active in the Cd-sensitive variety, SY33. Therefore, these results also suggest that antioxidant systems are first respond to Cd stress in maize plants, and sucrose metabolism is cooperation and complement that are exposed to Cd.

scholarly journals Gene expression in human small intestinal mucosa in vivo is mediated by iron-induced oxidative stress

2006 ◽  
Vol 25 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Freddy J. Troost ◽  
Robert-Jan M. Brummer ◽  
Guido R. M. M. Haenen ◽  
Aalt Bast ◽  
Rachel I. van Haaften ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Lipid Peroxidation ◽  
Small Intestine ◽  
Antioxidant Capacity ◽  
Intestinal Mucosa ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Oxidative Challenge ◽  
Gene Reporters

Iron-induced oxidative stress in the small intestine may alter gene expression in the intestinal mucosa. The present study aimed to determine which genes are mediated by an iron-induced oxidative challenge in the human small intestine. Eight healthy volunteers [22 yr(SD2)] were tested on two separate occasions in a randomized crossover design. After duodenal tissue sampling by gastroduodenoscopy, a perfusion catheter was inserted orogastrically to perfuse a 40-cm segment of the proximal small intestine with saline and, subsequently, with either 80 or 400 mg of iron as ferrous gluconate. After the intestinal perfusion, a second duodenal tissue sample was obtained. Thiobarbituric acid-reactive substances, an indicator of lipid peroxidation, in intestinal fluid samples increased significantly and dose dependently at 30 min after the start of perfusion with 80 or 400 mg of iron, respectively ( P < 0.001). During the perfusion with 400 mg of iron, the increase in thiobarbituric acid-reactive substances was accompanied by a significant, momentary rise in trolox equivalent antioxidant capacity, an indicator of total antioxidant capacity ( P < 0.05). The expression of 89 gene reporters was significantly altered by both iron interventions. Functional mapping showed that both iron dosages mediated six distinct processes. Three of those processes involved G-protein receptor coupled pathways. The other processes were associated with cell cycle, complement activation, and calcium channels. Iron administration in the small intestine induced dose-dependent lipid peroxidation and a momentary antioxidant response in the lumen, mediated the expression of at least 89 individual gene reporters, and affected at least six biological processes.

scholarly journals Métodos de aplicação de diferentes concentrações de H2O2 em milho sob estresse salino

2016 ◽  
Vol 11 (3) ◽  
pp. 01
Author(s):  
Evandro Manoel da Silva ◽  
Francisco Hélio Dantas Lacerda ◽  
Aldair de Souza Medeiros ◽  
Leandro de Pádua Souza ◽  
Francisco Hevilásio Freire Pereira
Keyword(s):  
Salt Stress ◽  
Irrigation Water ◽  
Antioxidant Defense ◽  
Saline Water ◽  
Foliar Spray ◽  
Antioxidant Defense System ◽  
Experimental Unit ◽  
Initial Growth ◽  
Maize Plants ◽  
Hybrid Maize

<p>A aplicação exógena de H<sub>2</sub>O<sub>2</sub> tem demonstrado eficiência na aclimatação das plantas aos estresses bióticos e abióticos devido estimular a ativação do sistema de defesa antioxidativo. Neste sentido, objetivou-se com o trabalho avaliar a influência do método de aplicação de diferentes concentrações de H<sub>2</sub>O<sub>2 </sub>sobre o crescimento inicial de plantas de milho irrigadas água salina. O experimento foi conduzido em ambiente protegido, com os tratamentos dispostos em delineamento inteiramente casualizado, em esquema fatorial 5 x 2, correspondente as concentrações de peróxido de hidrogênio (H<sub>2</sub>O<sub>2</sub>) de 0; 5; 10; 15 e 20 µmol L<sup>-1 </sup>aplicados na semeadura via água de irrigação e, pulverização foliar aos 15 dias após a semeadura (DAS), com quatro repetições e unidade experimental constituída por um vaso de 3 L contendo duas plantas de milho híbrido 4051. As plantas foram irrigadas com água de CE de 2,0 dS m<sup>-1</sup>. As 28 DAS verificou-se que a aplicação de H<sub>2</sub>O<sub>2 </sub>na semeadura e através de pulverização foliar promove aclimatação de plantas de milho à salinidade da água de irrigação, aumentando a tolerância ao estresse salino. O maior crescimento inicial do milho é obtido nas concentrações de H<sub>2</sub>O<sub>2</sub> variando de 7 a 8 µmol L<sup>-1</sup>, sendo mais eficiente a aplicação na semeadura. O pré-tratamento de plantas de milho com H<sub>2</sub>O<sub>2</sub> a partir de 15 µmol L<sup>-1</sup> incrementa o estresse, promovendo maiores danos ao crescimento vegetativo.</p><p align="center"><strong><em>Application methods of different concentrations of H<sub>2</sub>O<sub>2</sub> in maize under salt stress</em></strong></p><p><strong>Abstract</strong><strong>:</strong> Exogenous application of H<sub>2</sub>O<sub>2</sub> has established efficiency in acclimatization of plants to biotic and abiotic stresses due to stimulate the activation of antioxidant defense system. In this context, it was aimed with this work evaluate the effect of application method of different concentrations of H<sub>2</sub>O<sub>2</sub> on initial growth of maize plants irrigated saline water. The experiment was conducted in a greenhouse, with the treatments in a randomized completely design in a factorial 5 x 2 corresponding the hydrogen peroxide concentrations (H<sub>2</sub>O<sub>2</sub>) 0; 5; 10; 15:20 µmol L<sup>-1</sup> applied in sowing by irrigation water and foliar spray at 15 days after sowing (DAS), with four repetitions and experimental unit consisting of vessel a 3 L containing two plants hybrid maize 4051. The plants were irrigated with EC water of 2.0 dS m<sup>-1</sup>. The 28 DAS  it was found what the application H<sub>2</sub>O<sub>2</sub> at sowing and foliar spraying promote acclimatization of maize plants at salinity irrigation water, increasing tolerance to salt stress.  The more initial growth of maize is obtained in H<sub>2</sub>O<sub>2</sub> concentrations ranging from 7 to 8 µmol L<sup>-1</sup>, being more efficient the application at sowing. The pretreatment of maize plants with H<sub>2</sub>O<sub>2</sub> from 15 µmol L<sup>-1</sup> increasing stress, promoting further damage to vegetative growth.</p>

scholarly journals Maize crop yield in function of salinity and mulch

2021 ◽  
Vol 25 (12) ◽  
pp. 840-846
Author(s):  
Francisco H. R. Costa ◽  
Geovana F. Goes ◽  
Murilo de S. Almeida ◽  
Clarissa L. Magalhães ◽  
José T. M. de Sousa ◽  
...  
Keyword(s):  
Grain Yield ◽  
Irrigation Water ◽  
Saline Water ◽  
Block Design ◽  
Agronomic Performance ◽  
Maize Crop ◽  
Randomized Block Design ◽  
Salinity Levels ◽  
Maize Plants ◽  
Maize Grain

ABSTRACT Irrigation with saline water affects the agronomic performance of the maize crop; however, the use of vegetal mulch may mitigate salt stress and promote an increase in yield. In this way, this study aimed to evaluate the grain yield of the maize plants submitted to different water salinity levels in the presence and absence of mulch. The experiment was conducted in a randomized block design arranged in a 2 × 2 factorial scheme. The first factor was the salinity of the irrigation water (1.0 and 4.0 dS m-1) and the second, with and without mulch, and five replicates. The variables analyzed were: unhusked ear mass, husked ear mass, cob mass, straw mass, husked ear diameter, husked ear length, and yield. The irrigation water with higher electrical conductivity affects negatively the ear mass with and without straw, ear diameter and ear length. The use of vegetation cover on the soil increased the unhusked ear mass with and without straw, ear diameter and length. The water with higher salinity (4.0 dS m-1) reduces the maize grain yield but with less intensity in the presence of mulch.

scholarly journals Correlation between intermediary metabolism, Hsp gene expression, and oxidative stress-related proteins in long-term thermal-stressed Mytilus galloprovincialis

2020 ◽  
Vol 319 (3) ◽  
pp. R264-R281
Author(s):  
Konstantinos Feidantsis ◽  
Ioannis A. Giantsis ◽  
Andreas Vratsistas ◽  
Stavroula Makri ◽  
Athanasia-Zoi Pappa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Thermal Stress ◽  
Mytilus Galloprovincialis ◽  
Intermediary Metabolism ◽  
Related Proteins ◽  
And Oxidative Stress

Long-term exposure of Mytilus galloprovincialis to temperatures beyond 26°C triggers mussel mortality. The present study aimed to integratively illustrate the correlation between intermediary metabolism, hsp gene expression, and oxidative stress-related proteins in long-term thermally stressed Mytilus galloprovincialis and whether they are affected by thermal stress magnitude and duration. We accordingly evaluated the gene expression profiles, in the posterior adductor muscle (PAM) and the mantle, concerning heat shock protein 70 and 90 ( hsp70 and hsp90), and the antioxidant defense indicators Mn-SOD, Cu/Zn-SOD, catalase, glutathione S-transferase, and the metallothioneins mt-10 and mt-20. Moreover, we determined antioxidant enzyme activities, oxidative stress through lipid peroxidation, and activities of intermediary metabolism enzymes. The pattern of changes in relative mRNA expression levels indicate that mussels are able to sense thermal stress even when exposed to 22°C and before mussel mortality is initiated. Data indicate a close correlation between the magnitude and duration of thermal stress with lipid peroxidation levels and changes in the activity of antioxidant enzymes and the enzymes of intermediary metabolism. The gene expression and increase in the activities of antioxidant enzymes support a scenario, according to which exposure to 24°C might trigger reactive oxygen species (ROS) production, which is closely correlated with anaerobic metabolism under hypometabolic conditions. Increase and maintenance of oxidative stress in conjunction with energy balance disturbance seem to trigger mussel mortality after long-term exposure at temperatures beyond 26°C. Eventually, in the context of preparation for oxidative stress, certain hypotheses and models are suggested, integrating the several steps of cellular stress response.

scholarly journals Monolluma quadrangulaProtects against Oxidative Stress and Modulates LDL Receptor and Fatty Acid Synthase Gene Expression in Hypercholesterolemic Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
May N. Bin-Jumah
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Lipid Peroxidation ◽  
Fatty Acid ◽  
Proinflammatory Cytokines ◽  
Fatty Acid Synthase ◽  
Heart Function ◽  
Ldl Receptor ◽  
Antioxidant Defenses ◽  
Creatine Kinase Mb

Hypercholesterolemia is a metabolic disorder associated with oxidative stress. The present study investigated the protective effect ofMonolluma quadrangulaextract on hypercholesterolemia-induced oxidative stress in the liver and heart of high-cholesterol-diet- (HCD-) fed rats. The experimental animals received HCD for 10 weeks and were concurrently treated with 300 or 600 mg/kgM. quadrangulaextract. HCD-fed rats showed a significant increase in serum triglycerides, total cholesterol, LDL-cholesterol, vLDL-cholesterol, and cardiovascular risk indices along with decreased HDL-cholesterol and antiatherogenic index. TheM. quadrangulaextract significantly improved dyslipidemia and atherogenesis in HCD-fed rats. HCD induced a significant increase in serum transaminases, creatine kinase-MB, and proinflammatory cytokines. In addition, HDC induced a significant increase in hepatic and cardiac lipid peroxidation and decreased antioxidant enzymes. Treatment with theM. quadrangulaextract significantly alleviated liver and heart function markers, decreased proinflammatory cytokines and lipid peroxidation, and enhanced the antioxidant defenses. Also, theM. quadrangulaextract significantly reduced the expression of fatty acid synthase (FAS) and increased the expression of LDL receptor in the liver of HCD-fed rats. In conclusion, theM. quadrangulaextract has a potent antihyperlipidemic and cholesterol-lowering effect on HCD-fed rats. The beneficial effects of theM. quadrangulaextract were mediated through the increased antioxidant defenses, decreased inflammation and lipid peroxidation, and modulated hepatic FAS and LDL receptor gene expression.

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