scholarly journals Freeform 3D Bioprinting Involving Ink Gelation by Cascade Reaction of Oxidase and Peroxidase: A Feasibility Study Using Hyaluronic Acid-Based Ink

Biomolecules ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1908
Author(s):  
Shinji Sakai ◽  
Ryohei Harada ◽  
Takashi Kotani
Keyword(s):  
Hyaluronic Acid ◽  
Three Dimensional ◽  
Cascade Reaction ◽  
Mouse Fibroblast ◽  
Choline Oxidase ◽  
3D Bioprinting ◽  
Physical Support ◽  
Good Shape ◽  
Supporting Material ◽  
Supporting Materials

Freeform bioprinting, realized by extruding ink-containing cells into supporting materials to provide physical support during printing, has fostered significant advances toward the fabrication of cell-laden soft hydrogel constructs with desired spatial control. For further advancement of freeform bioprinting, we aimed to propose a method in which the ink embedded in supporting materials gelate through a cytocompatible and rapid cascade reaction between oxidase and peroxidase. To demonstrate the feasibility of the proposed method, we extruded ink containing choline, horseradish peroxidase (HRP), and a hyaluronic acid derivative, cross-linkable by HRP-catalyzed reaction, into a supporting material containing choline oxidase and successfully obtained three-dimensional hyaluronic acid-based hydrogel constructs with good shape fidelity to blueprints. Cytocompatibility of the bioprinting method was confirmed by the comparable growth of mouse fibroblast cells, released from the printed hydrogels through degradation on cell culture dishes, with those not exposed to the printing process, and considering more than 85% viability of the enclosed cells during 10 days of culture. Owing to the presence of derivatives of the various biocompatible polymers that are cross-linkable through HRP-mediated cross-linking, our results demonstrate that the novel 3D bioprinting method has great potential in tissue engineering applications.

Conductive Nanomaterials used in Bioinks for 3D Bioprinting

Nano LIFE ◽  
2021 ◽  
Vol 11 (02) ◽  
pp. 2130005
Author(s):  
Sheba Goklany
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
Bioactive Molecules ◽  
3D Bioprinting ◽  
Biological Degradation ◽  
High Reproducibility ◽  
Tissue Constructs ◽  
Conductive Nanomaterials ◽  
Supporting Materials ◽  
The Ideal

Biofabrication for tissue engineering and regenerative medicine is a rapidly evolving field that incorporates bioprinting or bioassembly for the development of biologically functional products with structural organization using cells, bioactive molecules, and biomaterials. Bioprinting is a biofabrication technology that utilizes biomaterials, living cells, and supporting materials, called bioink, to generate three-dimensional tissue constructs. Bioprinting offers several advantages over traditional scaffolding and microengineering methods such as precise architecture control, high reproducibility, and versatility. The ideal bioink should possess appropriate structural, mechanical, gelation, rheological, chemical, biological, degradation, and biomimetic properties for the desired application of the final product. Several natural and synthetic bioinks have been developed and this review has focused on conductive nanomaterials that have been used in combination with hydrogel materials for bioink synthesis.

scholarly journals Biomaterials Based on Marine Resources for 3D Bioprinting Applications

Marine Drugs ◽  
2019 ◽  
Vol 17 (10) ◽  
pp. 555 ◽  
Author(s):  
Yi Zhang ◽  
Dezhi Zhou ◽  
Jianwei Chen ◽  
Xiuxiu Zhang ◽  
Xinda Li ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Regenerative Medicine ◽  
Three Dimensional ◽  
Biologically Active ◽  
Marine Resources ◽  
3D Bioprinting ◽  
Natural Polymers ◽  
Flexible Tool ◽  
Mechanical Integrity ◽  
Further Development

Three-dimensional (3D) bioprinting has become a flexible tool in regenerative medicine with potential for various applications. Further development of the new 3D bioprinting field lies in suitable bioink materials with satisfied printability, mechanical integrity, and biocompatibility. Natural polymers from marine resources have been attracting increasing attention in recent years, as they are biologically active and abundant when comparing to polymers from other resources. This review focuses on research and applications of marine biomaterials for 3D bioprinting. Special attention is paid to the mechanisms, material requirements, and applications of commonly used 3D bioprinting technologies based on marine-derived resources. Commonly used marine materials for 3D bioprinting including alginate, carrageenan, chitosan, hyaluronic acid, collagen, and gelatin are also discussed, especially in regards to their advantages and applications.

scholarly journals Artificial Tumor Microenvironments in Neuroblastoma

Cancers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1629
Author(s):  
Colin H. Quinn ◽  
Andee M. Beierle ◽  
Elizabeth A. Beierle
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Therapeutic Interventions ◽  
3D Bioprinting ◽  
Culture Studies ◽  
In Vivo Models ◽  
Novel Treatments ◽  
Tumor Microenvironments ◽  
Novel Approach

In the quest to advance neuroblastoma therapeutics, there is a need to have a deeper understanding of the tumor microenvironment (TME). From extracellular matrix proteins to tumor associated macrophages, the TME is a robust and diverse network functioning in symbiosis with the solid tumor. Herein, we review the major components of the TME including the extracellular matrix, cytokines, immune cells, and vasculature that support a more aggressive neuroblastoma phenotype and encumber current therapeutic interventions. Contemporary treatments for neuroblastoma are the result of traditional two-dimensional culture studies and in vivo models that have been translated to clinical trials. These pre-clinical studies are costly, time consuming, and neglect the study of cofounding factors such as the contributions of the TME. Three-dimensional (3D) bioprinting has become a novel approach to studying adult cancers and is just now incorporating portions of the TME and advancing to study pediatric solid. We review the methods of 3D bioprinting, how researchers have included TME pieces into the prints, and highlight present studies using neuroblastoma. Ultimately, incorporating the elements of the TME that affect neuroblastoma responses to therapy will improve the development of innovative and novel treatments. The use of 3D bioprinting to achieve this aim will prove useful in developing optimal therapies for children with neuroblastoma.

scholarly journals Three-Dimensional Printable Hydrogel Using a Hyaluronic Acid/Sodium Alginate Bio-Ink

Polymers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 794 ◽  
Author(s):  
Su Jeong Lee ◽  
Ji Min Seok ◽  
Jun Hee Lee ◽  
Jaejong Lee ◽  
Wan Doo Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Sodium Alginate ◽  
Three Dimensional ◽  
Fibroblast Cell ◽  
Chemical Crosslinking ◽  
Fibroblast Cell Line ◽  
Engineering Applications ◽  
Encapsulated Cells ◽  
Crosslinking Agents

Bio-ink properties have been extensively studied for use in the three-dimensional (3D) bio-printing process for tissue engineering applications. In this study, we developed a method to synthesize bio-ink using hyaluronic acid (HA) and sodium alginate (SA) without employing the chemical crosslinking agents of HA to 30% (w/v). Furthermore, we evaluated the properties of the obtained bio-inks to gauge their suitability in bio-printing, primarily focusing on their viscosity, printability, and shrinkage properties. Furthermore, the bio-ink encapsulating the cells (NIH3T3 fibroblast cell line) was characterized using a live/dead assay and WST-1 to assess the biocompatibility. It was inferred from the results that the blended hydrogel was successfully printed for all groups with viscosities of 883 Pa∙s (HA, 0% w/v), 1211 Pa∙s (HA, 10% w/v), and 1525 Pa∙s, (HA, 30% w/v) at a 0.1 s−1 shear rate. Their structures exhibited no significant shrinkage after CaCl2 crosslinking and maintained their integrity during the culture periods. The relative proliferation rate of the encapsulated cells in the HA/SA blended bio-ink was 70% higher than the SA-only bio-ink after the fourth day. These results suggest that the 3D printable HA/SA hydrogel could be used as the bio-ink for tissue engineering applications.

Generating adipose stem cell-laden hyaluronic acid-based scaffolds using 3D bioprinting via the double crosslinked strategy for chondrogenesis

2021 ◽  
Vol 124 ◽  
pp. 112072
Author(s):  
Swathi Nedunchezian ◽  
Parikshit Banerjee ◽  
Chih-Yun Lee ◽  
Su-Shin Lee ◽  
Che-Wei Lin ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hyaluronic Acid ◽  
3D Bioprinting ◽  
Adipose Stem Cell

Three-dimensional Printing-Driving Liver Therapies

2021 ◽  
Vol 28 ◽  
Author(s):  
Xiaohong Li ◽  
Liang Wen ◽  
Jiao Liu ◽  
Xiaohong Wang
Keyword(s):  
Liver Diseases ◽  
Effective Means ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Three Dimensional Printing ◽  
Bottle Neck ◽  
Magnetic Resonance Imaging Mri ◽  
End Stage ◽  
Bioartificial Livers ◽  
Dimensional Printing

: End-stage liver diseases have long been a threat to human health, and so far, the treatment of these diseases lacks of effective means. Allogenic organ transplantation has become the last straw for most of the patients with end-stage liver diseases. However, this technique has been greatly limited by the serious shortage of donors and other factors, such as immune rejection, drug syndrome, and high cost. Recently, the emergence of three-dimensional (3D) bioprinting technologies, together with the magnetic resonance imaging (MRI) and computed tomography (CT) techniques, has driven the rapid growth of this field toward liver therapies. There are several basic requirements for liver 3D bioprinting. From information collection of diseased livers, to 3D printing of liver substitutes (containing the major structural, material and functional characters), and to clinical applications, suitable ‘bioinks’ and ‘bioprinters’ have played essential roles. In this review, we highlight the advanced ‘bioinks’ and ‘bioprinters’ that have been used for vascularized and innervated liver tissue 3D bioprinting. Further studies for the incorporation of biliary networks in the bioartificial livers have been emphasized. It is expected that when all the bottle-neck problems for liver 3D bioprinting are solved, batch (i.e. mass) and personalized production of bioartificial livers will make it very easy to treat end-stage liver diseases.

Hydrogel-Based Bioinks for Three-Dimensional Bioprinting: Patent Analysis

2021 ◽  
Vol 7 (1) ◽  
pp. 3
Author(s):  
Ahmed Fatimi
Keyword(s):  
Tissue Engineering ◽  
Detailed Analysis ◽  
State Of The Art ◽  
Three Dimensional ◽  
Patent Analysis ◽  
The State ◽  
Driving Factors ◽  
3D Bioprinting ◽  
Preparation Methods

There are a variety of hydrogel-based bioinks commonly used in three-dimensional bioprinting. In this study, in the form of patent analysis, the state of the art has been reviewed by introducing what has been patented in relation to hydrogel-based bioinks. Furthermore, a detailed analysis of the patentability of the used hydrogels, their preparation methods and their formulations, as well as the 3D bioprinting process using hydrogels, have been provided by determining publication years, jurisdictions, inventors, applicants, owners, and classifications. The classification of patents reveals that most inventions intended for hydrogels used as materials for prostheses or for coating prostheses are characterized by their function or properties Knowledge clusters and expert driving factors show that biomaterials, tissue engineering, and biofabrication research is concentrated in the most patents.

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