scholarly journals Evaluation of Anti-Obesity Activity, Acute Toxicity, and Subacute Toxicity of Probiotic Dark Tea

Biomolecules ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 99 ◽  
Author(s):  
Wang Ling ◽  
Shungeng Li ◽  
Xingcai Zhang ◽  
Yongquan Xu ◽  
Ying Gao ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Metabolic Disorders ◽  
The Body ◽  
High Fat ◽  
Water Group ◽  
Subacute Toxicity ◽  
Infusion Group ◽  
Sd Rats ◽  
Group Ii

: Probiotic dark tea (PDT) is a novel kind of dark tea produced by fresh albino tea leaves and fermented with specific probiotics. Our study demonstrates that PDT can ameliorate high-fat diet-induced overweight and lipid metabolic disorders and shows no acute or subacute toxicity in Sprague-Dawley (SD) rats. Daily intragastric administration of 5% PDT infusion for 14 days caused no obvious effect on general physiological features and behaviors of rats. Oral administration of 1%, 2%, and 3% of PDT infusion for six weeks had no influence on the biochemistry and histopathology of rats’ organs and blood, as well as the body weight and ratios of organ/body weight. To investigate its anti-obesity activity, SD rats were randomly divided into four groups, treated with normal diet + water (Group I), high-fat diet + water (Group II), high-fat diet + 3% traditional dark tea infusion (Group III), high-fat diet + 3% PDT infusion (Group IV). After six weeks, the body weight, serum total triacylglycerol (TG) and serum total cholesterol (TC) levels of rats in Group II were significantly increased and the high-density lipoprotein cholesterol (HDL) levels were significantly decreased compared with those in the other three groups. Both traditional dark tea and PDT treatment effectively counteracted the adverse effect of a high-fat diet in SD rats. These results suggest that PDT could be applied for the prevention of obesity, which ameliorates overweight and lipid metabolic disorders and which shows no acute or subacute toxicity.

scholarly journals Maternal High Fat Diet Consumption Exaggerates Metabolic Disorders in Mice With Cigarette-Smoking Induced Intrauterine Undernutrition

2021 ◽  
Vol 8 ◽  
Author(s):  
Taida Huang ◽  
Mo Yang ◽  
Yunxin Zeng ◽  
Xiaomin Huang ◽  
Nan Wang ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Metabolic Disorder ◽  
Maternal Smoking ◽  
Metabolic Disorders ◽  
The Body ◽  
Adverse Impact ◽  
Adult Offspring ◽  
High Fat ◽  
Gestation Age

Objectives: Maternal smoking causes fetal underdevelopment and results in births which are small for gestation age due to intrauterine undernutrition, leading to various metabolic disorders in adulthood. Furthermore, postnatal high fat diet (HFD) consumption is also a potent obesogenic factor, which can interact with maternal smoking. In this study, we aimed to determine whether maternal HFD consumption during pregnancy can reverse the adverse impact of maternal smoking and change the response to postnatal HFD consumption.Methods: Female mice were exposed to cigarette smoke (SE, 2 cigarettes/day) or sham exposed for 5 weeks before mating, with half of the SE dams fed HFD (43% fat, SE+HFD). The same treatment continued throughout gestation and lactation. Male offspring from each maternal group were fed the same HFD or chow after weaning and sacrificed at 13 weeks.Results: Maternal SE alone increased body weight and fat mass in HFD-fed offspring, while SE+HFD offspring showed the highest energy intake and glucose metabolic disorder in adulthood. In addition, postnatal HFD increased the body weight and aggravated the metabolic disorder caused by maternal SE and SE+HFD.Conclusions: Maternal HFD consumption could not ameliorate the adverse effect of maternal SE but exaggerate metabolic disorders in adult offspring. Smoking cessation and a healthy diet are needed during pregnancy to optimize the health outcome in the offspring.

Retarding effect of dietary fibers from bamboo shoot (Phyllostachys edulis) on hyperlipidemic rats induced by high-fat diet

2021 ◽  
Author(s):  
Qi Li ◽  
Xiangjun Fang ◽  
Hangjun Chen ◽  
Yanchao Han ◽  
Ruiling Liu ◽  
...  
Keyword(s):  
Body Weight ◽  
Dietary Fiber ◽  
High Fat Diet ◽  
The Body ◽  
Bamboo Shoot ◽  
Dietary Fibers ◽  
High Fat ◽  
Phyllostachys Edulis ◽  
Retarding Effect ◽  
Sd Rats

The effects of administration of bamboo shoot (Phyllostachys edulis) dietary fiber (BSDF) on high-fat diet (HDF) induced hyperlipidemia were studied with SD rats model. Results indicated that the body weight...

DHA-enriched phospholipids from large yellow croaker roe regulate lipid metabolic disorders and gut microbiota imbalance on SD rats with a high-fat diet

2021 ◽  
Author(s):  
Xiaodan Lu ◽  
Rongbin Zhong ◽  
Ling Hu ◽  
Luyao Huang ◽  
Lijiao Chen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Gut Microbiota ◽  
Polyunsaturated Fatty Acids ◽  
High Fat Diet ◽  
Nutritional Value ◽  
Metabolic Disorders ◽  
Large Yellow Croaker ◽  
High Fat ◽  
Sd Rats

Abstract Large yellow croaker roe phospholipids (LYCRPLs) has great nutritional value because of containing rich docosahexaenoic acid (DHA), which is a kind of n-3 polyunsaturated fatty acids (n-3 PUFAs). In...

scholarly journals Antiobesity and Hypolipidemic Activity of Moringa oleifera Leaves against High Fat Diet-Induced Obesity in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Souravh Bais ◽  
Guru Sewak Singh ◽  
Ramica Sharma
Keyword(s):  
Body Weight ◽  
Body Temperature ◽  
Total Cholesterol ◽  
High Fat Diet ◽  
Moringa Oleifera ◽  
Chronic Administration ◽  
The Body ◽  
Atherogenic Index ◽  
High Fat ◽  
Diet Induced Obesity

In the present study, the methanolic extract of Moringa oleifera leaves (MEMOL) was evaluated for antiobesity activity in rats. The antiobesity potential of MEMOL was studied against high fat diet-induced obesity (HFD) in rats. In this study, chronic administration of HFD in rats produced hypercholesterolemia (116.2 ± 0.27 mg/dL), which led to an increase in the body weight (225 gr), total cholesterol, triglycerides (263.0 ± 4.69 mg/dL), and attenuation in the levels of HDL (34.51 ± 2.20 mg/dL) as well as changes in body temperature of animals. Treatment of obese rats with MEMOL for 49 days resulted in a significant (P<0.001) change in body weight, total cholesterol, triglycerides, and LDL level along with a significant (P<0.001) increase in body temperature as compared to the HFD-induced obesity. MEMOL treated rats also showed a significant decrease in the level of liver biomarkers, organ weight, and blood glucose level. Further, rats treated with MEMOL (200 mg and 400 mg/kg) show reduced atherogenic index (1.7 ± 0.6 and 0.87 ± 0.76). The results indicate that the rats treated with Moringa oleifera (MO) have significantly attenuated the body weight without any change in the feed intake and also elicited significant thermogenic effect and to act as hypolipidemic and thermogenic property in obesity related disorders.

scholarly journals Anti-obesity Effect of (8-E)-Nüzhenide, a Secoiridoid from Ligustrum lucidum, in High-fat Diet-induced Obese Mice

2014 ◽  
Vol 9 (10) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Qing Liu ◽  
Sang Hyun Kim ◽  
Seon Beom Kim ◽  
Yang Hee Jo ◽  
Eun Sil Kim ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Body Weight Gain ◽  
Treated Group ◽  
The Body ◽  
Metabolic Parameters ◽  
Obese Mice ◽  
High Fat ◽  
Ligustrum Lucidum ◽  
Epididymal Fat

The effect of the extract of Ligustrum lucidum fruits (LFE) and its major secoiridoid (LFS), (8- E)-nüzhenide, on obesity was investigated using high fat-diet (HFD)-induced C58BL/6J obese mice. LFE and LFS were administered at the doses of 300 mg/kg and 30 mg/kg, respectively, for 6 weeks. The anti-obesity activity was evaluated by measuring body weight, epididymal fat and metabolic plasma parameters. On Day 42, the body weight of the LFS-treated group was significantly lower compared with the HFD-treated group. Body weight gain was also reduced by 23.2% and 32.0% in the LFE- and LFS-treated groups, respectively, compared with the HFD group. In addition, the weight of the epididymal fat in the mice was significantly decreased in the HFD+LFS group. The food efficiency ratios (FERs) of the HFD+LFE and HFD+LFS groups were also lower compared with the HFD group with the same food intake. Metabolic parameters that had increased in the HFD group were decreased in the HFD+LFE and HFD+LFS groups. In particular, the increased triglyceride values were significantly reduced in the HFD+LFS group. These results show that treatment with LFE and LFS decreased HFD-induced obesity, mainly by improving metabolic parameters, such as fats and triglycerides. Therefore, LFE and LFS have potential benefits in regulation of obesity.

scholarly journals The Effects of Triacylglycerol Structures on the Food Intake and Body Weight of Mice in a High-Fat Diet Setting

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1216-1216
Author(s):  
Xinge Hu
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
High Fat Diet ◽  
Tissue Sample ◽  
Body Weight Gain ◽  
Diet Group ◽  
The Body ◽  
Lower Percentage ◽  
Chow Diet ◽  
High Fat

Abstract Objectives The dietary fat content plays an important role in the regulation of chronic metabolic diseases such as obesity and type 2 diabetes. Here, we tested the impacts of triacylglycerol structure on the body weight gain and food intake of mice in a high-fat diet (HFD) setting. Methods Male C57/BL6J mice at 6 weeks old were fed one of the following three diets for 6 weeks, Teklad Rodent Diet chow diet (number 8640), the chow diet containing 36% (w/w) 1,2-Dipalmitoyl-3-oleoylglycerol (PPO), or the chow diet containing 36% (w/w) 1,3-Dipalmitoyl-2-oleoylglycerol (POP). Each group contained 9 mice, and their food intake and BW were measured daily. The mice were euthanized after 6 weeks (12 weeks old) for tissue sample collection. Results Both high HFD groups had significantly higher BW gain and caloric intakes than the chow diet group. Mice fed the POP diet had a lower percentage of BW gain and consumed less accumulated calories than those fed the PPO diet, as well as a significantly lower liver to BW ratio. Since week 4, the body BW rate of the POP group started to be lower than that of the PPO diet group. Conclusions TAG structures in an HFD setting affect the BW gain rate and obesity in mice. The different structures of fat added to affect the food intake and BW gain differently in an HFD setting. In the future, we would like to compare the changes of the hepatic lipogenesis enzyme in these mice. This will help us to understand how the triacylglycerol structures in the diet affect lipid metabolism in mice. Funding Sources Internal.

scholarly journals The Influence of High-Fat Diet in Early Life on Intestinal Tumorigenesis in APC1638N Mice

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 272-272
Author(s):  
Ting-Chun Lin ◽  
Ying Tang ◽  
Soonkyu Chung ◽  
Young-Cheul Kim ◽  
Zhenhua Liu
Keyword(s):  
Body Weight ◽  
Wnt Signaling ◽  
Tumor Size ◽  
High Fat Diet ◽  
Early Life ◽  
The Body ◽  
Tumor Incidence ◽  
High Fat ◽  
Intestinal Tumorigenesis ◽  
Female Mice

Abstract Objectives Colorectal cancer (CRC) is one of the most prevalent cancer worldwide. Evidence from epidemiological studies shows that the incidence rate of CRC among elders with age ≥ 50 years is gradually decreased, whereas the rate continuously rise in adults with age &lt; 50 years. Along with the rise of CRC in young adults, a significantly increasing trend in obesity is also observed in youth. The present study aims to investigate how the early-life nutrition influences the intestinal tumorigenesis later in mouse with an age equivalent to an age &lt; 50 years in human. Methods APC1638N mice (4 weeks of age) were fed a low-fat diet (N = 22; LF: 10% kcal from fat) or a high-fat diet (N = 23; HF: 60% kcal from fat) for 8 weeks, which is equivalent to child/adolescent age in humans. After that, all animals were switched to standard chow diet (LabDiet #5P76) and fed for additional 12 weeks before sacrifice. Tumors were examined and the expression tumorigenic Wnt-signaling downstream genes (Cyclin D1, c-Myc and Axin 2) in the intestine were assessed. Results Our results showed that compared to LF group, the body weight of both male and female mice significantly increased after 8-week HF feeding (P &lt; 0.05). After switching to the standard chow diet for further 12 weeks feeding, the increase of body weight in HF group remained, although the degree of magnitude reduced, and a statistical significance only shown in female mice (P &lt; 0.05). There were a higher tumor incidence (P = 0.051) and tumor multiplicity (P &lt; 0.05) in males than female.  No interactions between gender and diet were observed. The HF group had a higher tumor incidence (P = 0.088) and tumor size (P &lt; 0.05) when compared to the LF group. The expression of Wnt-signaling downstream gene, c-Myc, was significantly increased in the HF group at 24-week of age (P &lt; 0.01). Conclusions A short term of high-fat diet in early life tends to promote intestinal tumorigenesis in adults as indicated by a mild increase in tumor incidence and a significant increase in tumor size. Funding Sources This project was supported by the US Department of Agriculture Hatch funding (#1013548).

scholarly journals The Correlation of Weight and Blood Cholesterol Levels of White Rat (Rattus Norvegicus) with High-Fat Diet

2016 ◽  
Vol 3 (3) ◽  
pp. 202-206
Author(s):  
Thatit Nurmawati
Keyword(s):  
Body Weight ◽  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Egg Yolk ◽  
The Body ◽  
Blood Cholesterol ◽  
High Fat ◽  
Data Measurement ◽  
Cholesterol Levels

Cholesterol is an essential substance for the body. The role of cholesterol as material hormones,cell membranare needed by the body. This conditionchanges into a distrubtion if the cholesterollevels in the blood increase. Weight becomes one of this trigger. The consumption of high-fat foodsincrease weight which resulting in the increase of cholesterol cases. The purpose of this study was todetermine the level of correlations between weight and cholesterol levels after being given a high-fatdiet.The study used rats (Rattus norvegicus) sex male, 16 rats with age between 1-2 months. Rats weightrange between 100-150 gr and in healthy conditions. The giving of high-fat diet were in the form ofchicken feed, duck eggs, goat oil, lard and flour for 8 weeks. The data measurement done by scales andmeasuringcholesterol levels through the end of the tail by means of easy touch. The data analysis weredone to understand level of correlation between variables. The presentation of the data used tables. Theresults showed body weight of rats did not change after administration of a high-fat diet. The cholesterolslevels of the subjects were high. Theadministration of high-fat diet from egg yolk dan goat oilcouldincrease the level of cholesterol. There was a correlation between weight and cholesterol levels afterbeing given a high-fat diet (p <0.5). It was needed to repeatthe measurements to determine changes incholesterol levels and other factors that affect thigh blood to cholesterol levels.

scholarly journals Anti-obesity effect of Tamarindus indicus seed extract against a high-fat diet-induced obese model in rats

2020 ◽  
Vol 11 (2) ◽  
pp. 2083-2089
Author(s):  
Nabeel K ◽  
Asra Fathima ◽  
Farhath Khanum ◽  
Manjula S N ◽  
Mruthunjaya K ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Body Weight Gain ◽  
Seed Extract ◽  
The Body ◽  
High Fat ◽  
Cell Viability Assay ◽  
Serum Triglycerides

The present study was aimed to evaluate the anti-obesity property of Tamarindus indica seed extract (TSE) on high fat-fed obese rats. TSE was prepared by cold maceration method and qualitative phytochemical studies had been carried out. In vitro cell viability assay (MTT assay) was and oil red staining for evaluating the lipid accumulation in cells was carried out using 3T3-L1 cells, and leptin levels was evaluated by ELISA. In-vivo Obesity was induced in experimental rats by administration of a high-fat diet for 04 weeks. The anti-obesity effect was screened by oral administration of TSE at two different dose levels i.e., 250 and 500mg/kg b. Wt. Along with a high-fat diet for a period of 04 weeks. The anti-obesity activity is estimated in terms of body weight gain, serum triglycerides (TG), Total cholesterol (TC). In -vitro studies revealed that the TSE has no cytotoxic effect, Administration of a high-fat diet for 04 weeks significantly increased the body weight, serum triglycerides, cholesterol. Upon treatment with TSE, a significant dose-dependent alteration in body weight, triglycerides, cholesterol levels were observed, inferring the anti-obesity property of Tamarindus seed extract.

scholarly journals PO-224 Effect of high-fat diet on body weight and spontaneous physical activity of SD rats

2018 ◽  
Vol 1 (5) ◽  
Author(s):  
Shanshan Cao ◽  
Wei Chen ◽  
Juan Li ◽  
Yuxiu He
Keyword(s):  
Physical Activity ◽  
Body Weight ◽  
High Fat Diet ◽  
Activity Level ◽  
Control Group ◽  
Activity Levels ◽  
High Fat ◽  
Exercise Time ◽  
Sd Rats ◽  
Physical Activity Levels

Objective  Excessive intake of high-energy foods and insufficient levels of physical activity are important causes of obesity. In addition, inadequate physical activity is also a major cause of cardiovascular disease and type 2 diabetes. Relevant data suggests that most adults fail to achieve the level of physical activity needed to improve their health. Therefore, understanding the reasons for the lack of physical activity levels is essential for developing a reduction in sedentary and thus preventing chronic acute illnesses. It is well known that physical activity is good for health, but little is known about the genetic and biological factors that may affect this complex behavior. Some studies have shown that diet-induced obesity may alter dopaminergic activity and thus reduce physical activity levels, suggesting that obesity and diet may be inversely related to dopamine signaling. Therefore, it is necessary to further study the correlation between obesity, dopamine and physical activity levels, and to explore the relationship between high-fat diet and body weight changes and physical activity levels. Methods  Sixteen male Sprague-Dawley rats were randomly divided into two groups. The control group (n=8) was fed with basal diet for 8 weeks, and the high-fat group (n=8) was fed with high-fat diet for 8 weeks. To compare the difference in body weight and physical activity between SD rats fed with high-fat diet and normal diet, and the relationship between body weight and body activity level; in order to study the effect of obesity on exercise behavior, use the open field experimental recorder for each The movements of the rats in the group were recorded (autonomic activity for 30 min), and the correlation between the effects of high-fat diet on body weight and spontaneous activities of SD rats was analyzed. Results High-fat diet and normal-fed rats were in energy intake (high-fat group 4583.94±349.85; control group 3201±298.58), body weight (high-fat group 406.23±29.35; control group 306.66±31.44) and Lee's index (high-fat group 26.17 ± 0.57; control group 24.35 ± 0.97) were significantly different. There was a high correlation between energy intake and body weight in rats, correlation coefficient r=0.911 (p<0.01); correlation coefficient between body weight and physical activity level r = 0.576 (p < 0.05). In addition, by comparing the exercise time and average speed of rats in each group, the difference in exercise time between the two groups was not significant, and the average speed difference was significant (p<0.05); exercise time was significantly correlated with physical activity level, r= 0.734 (p<0.01); and the mean speed was also positively correlated with physical activity level, and the correlation coefficient was 0.660 (P<0.01). Conclusions Obesity is greatly affected by dietary factors, and long-term high-fat diets lead to a decline in physical activity, which in turn promotes further deterioration of obesity. This interaction can create a vicious circle between obesity and physical activity. Further research on the mechanisms of obesity, lack of physical activity and their interaction may provide a theoretical basis for increasing the level of physical activity in obese people.

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