scholarly journals Antioxidant Barrier, Redox Status, and Oxidative Damage to Biomolecules in Patients with Colorectal Cancer. Can Malondialdehyde and Catalase Be Markers of Colorectal Cancer Advancement?

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 637 ◽  
Author(s):  
Zińczuk ◽  
Maciejczyk ◽  
Zaręba ◽  
Romaniuk ◽  
Markowski ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Oxidative Damage ◽  
Redox Status ◽  
Control Group ◽  
Tumour Invasion ◽  
Reducing Ability ◽  
Glycation End Products ◽  
Total Antioxidant ◽  
Oxidative Damage To Proteins ◽  
Oxidant Status

This study is the first to assess the diagnostic utility of redox biomarkers in patients with colorectal cancer (CRC). Antioxidant barrier (Cu,Zn-superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), uric acid (UA), reduced glutathione (GSH)), redox status (total antioxidant (TAC)/oxidant status (TOS), ferric reducing ability (FRAP)), and oxidative damage products (advanced glycation end products (AGE), advanced oxidation protein products (AOPP), malondialdehyde (MDA)) were measured in serum/plasma samples of 50 CRC patients. The activity of SOD was significantly higher whereas the activity of CAT, GPx and GR was considerably lower in CRC patients compared to the control group (p < 0.0001). Levels of UA, TOS, and OSI and concentrations of AGE, AOPP, and MDA were significantly higher, and the levels of GSH, TAC, and FRAP were considerably lower in CRC patients compared to the healthy controls (p < 0.0001). AUC for CAT with respect to presence of lymph node metastasis was 0.7450 (p = 0.0036), whereas AUC for MDA according to the depth of tumour invasion was 0.7457 (p = 0.0118). CRC is associated with enzymatic/non-enzymatic redox imbalance as well as increased oxidative damage to proteins and lipids. Redox biomarkers can be potential diagnostic indicators of CRC advancement.

The Association between Oxidative Stress and Cardiac Functions in Infants Born to Preeclamptic Mothers

2018 ◽  
Vol 36 (11) ◽  
pp. 1205-1210
Author(s):  
Didem Arman ◽  
Secil Ercin ◽  
Sevilay Topcuoğlu ◽  
Ayşem Kaya ◽  
Taner Yavuz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Premature Infants ◽  
Stress Index ◽  
Control Group ◽  
Cardiac Functions ◽  
Total Antioxidant ◽  
Echocardiographic Parameters ◽  
Oxidant Status ◽  
The Relationship ◽  
And Oxidative Stress

Objective The present study aimed to assess the global oxidant and antioxidant status in infants born to preeclamptic mothers and their correlation with cardiac functions. Study Design We compared 40 infants born to preeclamptic mothers with 40 premature infants born to normotensive mothers. We assessed the relationship between echocardiographic measurements and total antioxidant capacity (TAC) and total oxidant status (TOS) values. Results In the study group, TAC, TOS, and oxidative stress index (OSI) levels were significantly higher in the cord blood (p = 0.03, 0.04, and 0.039, respectively) than in the control group. We did not observe any correlation between echocardiographic measurements and TAC, TOS, and OSI levels in infants born to preeclamptic mothers. Conclusion Compared with the control group, despite higher TAC levels in infants born to preeclamptic mothers, concurrent elevated OSI levels reveal that the oxidant–antioxidant balance is disturbed in favor of oxidants. Furthermore, the findings of this study suggest that echocardiographic parameters are unaffected by the oxidant status.

scholarly journals Salivary Biomarkers of Oxidative Stress in Children with Chronic Kidney Disease

2018 ◽  
Vol 7 (8) ◽  
pp. 209 ◽  
Author(s):  
Mateusz Maciejczyk ◽  
Julita Szulimowska ◽  
Anna Skutnik ◽  
Katarzyna Taranta-Janusz ◽  
Anna Wasilewska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Oxidative Damage ◽  
Diagnostic Value ◽  
Redox Status ◽  
Stress Index ◽  
Antioxidant Systems ◽  
Control Group ◽  
Potential Biomarker

There are still missing non-invasive biomarkers of chronic kidney disease (CKD) in children. Therefore, the aim of the study was to evaluate oxidative stress indicators in the non-stimulated (NWS) and stimulated saliva (SWS) of CKD children (n = 25) and healthy controls (n = 25). Salivary antioxidants (catalase (CAT), peroxidase (Px), superoxide dismutase (SOD), uric acid (UA), reduced glutathione (GSH), albumin), redox status (total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI)), and oxidative damage products (advanced glycation end products (AGE), advanced oxidation protein products (AOPP), malondialdehyde (MDA)) were evaluated. We have demonstrated the significantly higher activity of SWS GPx and SOD, as well as elevated concentrations of UA and albumin in NWS and SWS of CKD children vs. the control group. TAC, TOS and OSI were significantly higher only in SWS, while oxidative damage products (AGE, AOPP and MDA) were significantly higher in both NWS and SWS of CKD children. ROC analysis showed a considerably high diagnostic value of AOPP in both NWS and SWS of CKD children compared to controls (AUC = 0.92; 0.98). CKD is responsible for disturbances in salivary antioxidant systems and oxidative damage to proteins and lipids. Salivary AOPP can be a potential biomarker of CKD in children.

scholarly journals Salivary Gland Function, Antioxidant Defence and Oxidative Damage in the Saliva of Patients with Breast Cancer: Does the BRCA1 Mutation Disturb the Salivary Redox Profile?

Cancers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1501 ◽  
Author(s):  
Sawczuk ◽  
Maciejczyk ◽  
Sawczuk-Siemieniuk ◽  
Posmyk ◽  
Zalewska ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Salivary Gland ◽  
Oxidative Damage ◽  
Cancer Progression ◽  
Brca1 Mutation ◽  
Total Antioxidant Status ◽  
Redox Balance ◽  
Breast Cancer Patients ◽  
Total Antioxidant ◽  
Oxidative Damage To Proteins

: Oxidative stress plays a key role in breast cancer progression. However, little is still known about the relationship between the BRCA1 mutation, the incidence of breast cancer and oral homeostasis. This is the first study to evaluate the secretory function of salivary glands, biomarkers of redox balance, and oxidative damage to proteins and lipids in the saliva of subjects with the BRCA1 mutation. Ninety eight women were enrolled in the study and allocated to four groups based on molecular DNA testing: generally healthy patients without the BRCA1 mutation, patients with breast cancer but without the BRCA1 mutation, generally healthy patients with the BRCA1 mutation, and patients with both breast cancer and the BRCA1 mutation. We demonstrated that saliva from breast cancer patients with the BRCA1 mutation is characterized by enhanced antioxidant capacity and a higher degree of oxidative damage to proteins and lipids. The BRCA1 mutation can cause a predisposition to early salivary gland dysfunction, both in patients with breast cancer and in healthy individuals, leading to a decrease in salivary proteins. Using cluster analysis, we showed that salivary peroxidase, advanced glycation end-products (AGE), total antioxidant status (TAS) and malondialdehyde (MDA) may have particular clinical significance in non-invasive diagnostics of breast cancer.

Impact of the Ser326Cys polymorphism of the OGG1 gene on the level of oxidative DNA damage in patients with colorectal cancer

2018 ◽  
Vol 90 (2) ◽  
pp. 13-15 ◽  
Author(s):  
Jacek Kabzinski ◽  
Anna Walczak ◽  
Adam Dziki ◽  
Michał Mik ◽  
Ireneusz Majsterek
Keyword(s):  
Colorectal Cancer ◽  
Dna Damage ◽  
Oxidative Damage ◽  
Oxidative Dna Damage ◽  
Excision Repair ◽  
Genetic Material ◽  
Control Group ◽  
Ogg1 Gene ◽  
Increased Risk ◽  
Ser326cys Polymorphism

As a result of reactive oxygen species operation, cell damage occurs in both cellular organelles and molecules, including DNA. Oxidative damage within the genetic material can lead to accumulation of mutations and consequently to cancer transformation. OGG1 glycosylase, a component of the Base Excision Repair (BER) system, is one of the enzymes that prevents excessive accumulation of 8-oxoguanine (8-oxG), the most common compound formed by oxidative DNA damage. In case of structural changes of OGG1 resulting from polymorphic variants, we can observe a significant increase in the concentration of 8-oxG. Linking individual polymorphisms to DNA repair systems with increased risk of colorectal cancer will allow patients to be classified as high risk and included in a prophylactic program. The aim of the study was to determine the level of oxidative DNA damage and to analyze the distribution of Ser326Cys polymorphism of the OGG1 gene in a group of patients with colorectal cancer and in a control group in the Polish population. Material and methodology. DNA was isolated from the blood of 174 patients with colorectal cancer. The control group consisted of 176 healthy individuals. The level of oxidative damage was determined by analyzing the amount of 8-oxguanine using the HT 8-oxo-dG ELISA II Kit. Genotyping was performed via the TaqMan method. Results. The obtained results indicate that Ser326Cys polymorphism of the OGG1 gene increases the risk of RJG and is associated with significantly increased levels of 8-oxoguanine. Conclusions. Based on the results obtained, we conclude that Ser326Cys polymorphism of the OGG1 gene may modulate the risk of colorectal cancer by increasing the level of oxidative DNA damage.

scholarly journals Assessment of total (anti)oxidant status in goat kids

2021 ◽  
Vol 64 (1) ◽  
pp. 139-146
Author(s):  
Stefano Cecchini ◽  
Francesco Fazio
Keyword(s):  
Radical Scavenging Activity ◽  
Analytical Data ◽  
Radical Scavenging ◽  
Redox Balance ◽  
Reactive Oxygen Metabolites ◽  
Strongly Correlated ◽  
Antioxidant Assays ◽  
Reducing Ability ◽  
Total Antioxidant ◽  
Oxidant Status

Abstract. The redox potential of goat serum was assessed by different spectrophotometric assays. Among them, three methods are commonly applied for the evaluation of the oxidative (reactive oxygen metabolites, ROMs, and total oxidant status, TOS) and nitrosative (NO⚫ metabolites, NOx) stress, and four methods for the evaluation of the antioxidant status: the total antioxidant capacity (TAC) based on the ferric reducing ability of plasma (FRAP), the total antioxidant activity (TAA) based on the reduction of the coloured ABTS⚫+ radical cation, the free radical scavenging activity (FRSA) based on the reduction of the purple DPPH⚫, and the total thiol levels (TTLs) based on their interaction with DTNB to form a highly coloured anion. Besides, myeloperoxidase (MPO) and ceruloplasmin oxidase (CP) activities were also assessed. Except for TAA, analytical data showed a great inter-individual variation for both oxidant and antioxidant assays. ROMs were strongly correlated with CP, while TOS with MPO and TAC. Furthermore, a tendency between TOS and FRSA was shown. NOx was correlated with TAC and TAA, and a tendency with TOS was shown. No correlations appeared among the antioxidant assays, even if a tendency between TAC and TAA was evidenced, but TAC was correlated with MPO activity. The observed correlation between ROMs and CP is discussed as a possible analytical interference. The absence of correlation among the antioxidant biomarkers suggests the simultaneous use of a panel of tests to verify any changes in the redox balance, mainly in livestock in which reference values for each biomarker are lacking.

scholarly journals The Effect of Lyophilized Pomegranate Extract on Epididymal Sperm Quality, Oxidative Stress and Spermatogenic Cell Density in Rabbits

2018 ◽  
Vol 69 (1) ◽  
pp. 723 ◽  
Author(s):  
F. AVDATEK ◽  
D. YENI ◽  
D. F. BAŞER ◽  
H. H. DEMIREL ◽  
Ö. HAZMAN ◽  
...  
Keyword(s):  
Cell Density ◽  
Carboxymethyl Cellulose ◽  
Membrane Integrity ◽  
Group Iv ◽  
Spermatogenic Cell ◽  
Control Group ◽  
Abnormal Sperm ◽  
Group I ◽  
Total Antioxidant ◽  
Oxidant Status

The aim of this study was to investigate the effect of lyophilized pomegranate extract on spermatological features, pathology of testes and total antioxidant/oxidant status in rabbits. Adult male rabbits were divided into four groups containing six rabbits each. For 8 weeks, Group I received standard diet and 1 ml % 0.5 carboxymethyl cellulose (CMC), Group II received 25 mg/kg/day pomegranate extract + 1 ml % 0.5 carboxymethyl cellulose (CMC), Group III received 50 mg/kg/day pomegranate extract + 1 ml % 0.5 CMC and Group IV received 100 mg/kg/day pomegranate extract + 1 ml % 0.5 CMC. Rabbits were sacrificed by using xylazine 5 mg/kg+ketamine 35 mg/kg anaesthesia and were euthanized by 150 mg / kg intraperitoneal thiopental sodium at the end of the eighth week. Spermatozoon motility, abnormal sperm rate, sperm membrane integrity, total antioxidant/oxidant level and spermatogenic cell density were investigated. All analyses were done only once at the end of study period. Data were compared by analysis of variance (ANOVA) and the degree of significance was set at (p<0.05). Sperm motility and membrane integrity increased significantly (p<0.05) in groups II, III and IV; abnormal sperm rate decreased significantly (p<0.05) in groups III, IV; total oxidant status decreased significantly (p<0.05) in group IV in comparison to the control group. Seminiferous tubule diameter increased significantly (p<0.05) in all groups compared to the control group. Germinal cell layer thickness significantly increased (p<0.05) in group IV compared to the control group. Results of this study suggest that 50mg/kg/day + 1 ml % 0.5 CMC and 100 mg/kg/day + 1 ml % 0.5 CMC improve sperm parameters in rabbits.

scholarly journals Dysfunction of Salivary Glands, Disturbances in Salivary Antioxidants and Increased Oxidative Damage in Saliva of Overweight and Obese Adolescents

2020 ◽  
Vol 9 (2) ◽  
pp. 548 ◽  
Author(s):  
Anna Zalewska ◽  
Agnieszka Kossakowska ◽  
Katarzyna Taranta-Janusz ◽  
Sara Zięba ◽  
Katarzyna Fejfer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Redox Homeostasis ◽  
Salivary Secretion ◽  
Control Group ◽  
Total Protein Content ◽  
Parotid Glands ◽  
Obese Adolescents ◽  
Total Antioxidant ◽  
And Gender

Obesity is inseparably connected with oxidative stress. This process may disturb the functioning of the oral cavity, although the effect of oxidative stress on salivary gland function and changes in the qualitative composition of saliva are still unknown. Our study is the first to evaluate salivary redox homeostasis in 40 overweight and obese adolescents and in the age- and gender-matched control group. We demonstrated strengthening of the antioxidant barrier (superoxide dismutase, catalase, peroxidase, uric acid, total antioxidant capacity (TAC)) with a simultaneous decrease in reduced glutathione concentration in saliva (non-stimulated/stimulated) in overweight and obese teenagers compared to the controls. The concentration of the products of oxidative damage to proteins (advanced glycation end products), lipids (malondialdehyde, 4-hydroxynonenal) and DNA (8-hydroxydeoxyguanosine) as well as total oxidative status were significantly higher in both non-stimulated and stimulated saliva as well as plasma of overweight and obese adolescents. Importantly, we observed more severe salivary and plasma redox alterations in obese adolescents compared to overweight individuals. In the study group, we also noted a drop in stimulated salivary secretion and a decrease in total protein content. Interestingly, dysfunction of parotid glands in overweight and obese teenagers intensified with the increase of BMI. We also showed that the measurement of salivary catalase and TAC could be used to assess the central antioxidant status of overweight and obese adolescents.

Total oxidant status and total antioxidant capacity in the saliva of patients with chronic periodontitis

MedPharmRes ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 21-26
Author(s):  
Thuy T. Nguyen ◽  
Duc A. Vo ◽  
Truc Thanh Thai ◽  
Thuy L. Vo ◽  
Nam C-N. Huynh
Keyword(s):  
Antioxidant Capacity ◽  
Chronic Periodontitis ◽  
Total Antioxidant Capacity ◽  
Control Group ◽  
Total Oxidant Status ◽  
Oxidative Stress Biomarkers ◽  
Clinical Attachment Loss ◽  
Attachment Loss ◽  
Total Antioxidant ◽  
Oxidant Status

Background: Recently, methods of quantifying total oxidant status (TOS) and total antioxidant capacity (TAOC) were developed to investigate periodontitis. This study was performed to evaluate the salivary TOS and TAOC levels of patients with chronic periodontitis (CP) and investigating the association between periodontal clinical parameters and these oxidative stress biomarkers. Material and methods: 40 participants (23-65 years old) were classified into two groups of 20 each, namely the CP group (participants with CP) and the control group (periodontally healthy controls). Clinical periodontal parameters were monitored, and TOS and TAOC levels were measured using laboratory assays. Results: TOS level increased in the saliva of patients with CP and the salivary TAOC in patients with CP was significantly lower than that of the control group. The TOS had a positive moderate correlation with the plaque index and clinical attachment loss (r=0.32 and 0.37, respectively) while TAOC was negatively and moderately correlated with clinical attachment loss (r=-0.35). Conclusion: Salivary TOS and TAOC were distinguished in healthy and chronic PD patients. Further studies are required to comprehensively evaluate the potential role of these biomarkers in diagnosis and treatment evaluation of CP.

Oxidative stress increases in carbon monoxide poisoning patients

2010 ◽  
Vol 30 (2) ◽  
pp. 160-164 ◽  
Author(s):  
Havva Sahin Kavakli ◽  
Ozcan Erel ◽  
Orhan Delice ◽  
Gizem Gormez ◽  
Semra Isikoglu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Monoxide ◽  
Antioxidant Status ◽  
Carbon Monoxide Poisoning ◽  
Total Antioxidant Status ◽  
Control Group ◽  
Total Oxidant Status ◽  
Oxidative Stress Parameters ◽  
Total Antioxidant ◽  
Oxidant Status

Objective: Carbon monoxide poisoning (COP) is one of the important causes of morbidity and mortality in toxicological cases. In this study, we aimed to find out more about the pathophysiology of COP by investigating the effects of COP on oxidative stress parameters such as total oxidant status (TOS) and total antioxidant status (TAS). Methods: Eighty-eight patients admitted to the emergency department of our hospital with acute COP and 35 healthy adults as control group were included in this study. Blood samples were collected from all COP patients at the time of initial emergency department evaluation to determine the oxidative stress parameters. Then, serum levels of total antioxidant status and total oxidant status levels were measured. Results: A total of 88 patients poisoned by carbon monoxide (CO; mean age 37.1 ± 18.2 years; 54’% women) were enrolled. TOS and carboxyhemoglobin (COHb) levels in COP patients were increased when compared to control group (p = 0.001). TOS, oxidative stress index (OSI) and COHb levels in COP patients were significantly lower after the treatment. (respectively, p = 0.016; p = 0.023; p = 0.001). On the other hand, no statistical differences were observed in TAS levels of study and control group as well as there were no changes with treatment. Conclusion: Measurements of TOS, TAS and OSI levels may be useful markers to find out the pathophsiology of COP.

scholarly journals Total Antioxidant Capacity, Catalase Activity and Salivary Oxidative Parameters in Patients with Temporomandibular Disorders

2020 ◽  
Author(s):  
Neda Omidpanah ◽  
Saba Ebrahimi ◽  
Asad Vaisi Raygani ◽  
Hadi Mozafari ◽  
Mansour Rezaei
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Temporomandibular Disorders ◽  
Catalase Activity ◽  
Total Antioxidant Capacity ◽  
Control Group ◽  
Healthy Controls ◽  
Oxidative Stress Biomarkers ◽  
Reducing Ability ◽  
Total Antioxidant

Objectives: Temporomandibular disorders (TMD) are characterized by pain or discomfort in the temporomandibular joint, periauricular region, masticatory muscles, and neck on one or both sides. It may also be associated with joint sounds, restricted mandibular movements and mandibular deviation. Oxidative agents may have a deleterious role in the pathogenesis of joint diseases, and oxidative stress can lead to TMD. The aim of this study was to assess the oxidative stress biomarkers in the saliva of TMD patients and healthy controls. Materials and Methods: This case-control study was conducted on 30 patients with TMDs (5 males and 25 females) with a mean age of 30.7±13.2 years, and 30 healthy controls (5 males and 25 females) with a mean age of 29.16±11.2 years. Saliva samples were collected according to the standard protocol and the total antioxidant capacity of the saliva (non-enzymatic), catalase activity, and malondialdehyde (MDA) levels were measured using the ferric reducing ability of plasma, Aebi’s method, and high-performance liquid chromatography, respectively. Finally, The MDA levels were analyzed by the Mann-Whitney test. Other quantitative parameters were analyzed by independent t-test.  Results: TMD patients had significantly higher salivary levels of MDA compared to the control group (P=0.001). But there were no significant differences in catalase (P=0.49) and total antioxidant capacity (P=0.22) of TMD patients and healthy controls. Conclusion: It seems that oxidative stress may be involved in the pathogenesis of TMDs.

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