scholarly journals Treatment with Luteolin Improves Lipopolysaccharide-Induced Periodontal Diseases in Rats

Biomedicines ◽  
2020 ◽  
Vol 8 (10) ◽  
pp. 442
Author(s):  
Giovanna Casili ◽  
Alessio Ardizzone ◽  
Marika Lanza ◽  
Enrico Gugliandolo ◽  
Marco Portelli ◽  
...  
Keyword(s):  
Biochemical Analysis ◽  
Fruits And Vegetables ◽  
Alveolar Bone ◽  
Periodontal Diseases ◽  
Well Being ◽  
Alveolar Bone Loss ◽  
Periodontal Tissue ◽  
Neutrophilic Infiltration ◽  
Natural Origin ◽  
Different Doses

Periodontitis is a dental disease that produces the progressive destruction of the bone surrounding the tooth. Especially, lipopolysaccharide (LPS) is involved in the deterioration of the alveolar bone, inducing the release of pro-inflammatory mediators, which cause periodontal tissue inflammation. Luteolin (Lut), a molecule of natural origin present in a large variety of fruits and vegetables, possess beneficial properties for human health. On this basis, we investigated the anti-inflammatory properties of Lut in a model of periodontitis induced by LPS in rats. Animal model predicted a single intragingival injection of LPS (10 μg/μL) derived from Salmonella typhimurium. Lut administration, was performed daily at different doses (10, 30, and 100 mg/kg, orally), starting from 1 h after the injection of LPS. After 14 days, the animals were sacrificed, and their gums were processed for biochemical analysis and histological examinations. Results showed that Lut (30 and 100 mg/kg) was equally able to reduce alveolar bone loss, tissue damage, and neutrophilic infiltration. Moreover, Lut treatment reduced the concentration of collagen fibers, mast cells degranulation, and NF-κB activation, as well as the presence of pro-inflammatory enzymes and cytokines. Therefore, Lut implementation could represent valid support in the pharmacological strategy for periodontitis, thus improving the well-being of the oral cavity.

scholarly journals Annual alveolar bone loss in older adults taking oral bisphosphonate: a retrospective cohort study

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Mohammad Helmi ◽  
Sara AlOsaimy ◽  
J. Max Goodson ◽  
Hatice Hasturk ◽  
Zuhair S. Natto
Keyword(s):  
Older Adults ◽  
Cohort Study ◽  
Bone Loss ◽  
Retrospective Cohort Study ◽  
Retrospective Cohort ◽  
Alveolar Bone ◽  
Periodontal Diseases ◽  
Alveolar Bone Loss ◽  
Oral Bisphosphonate ◽  
Associated Risk Factors

Abstract Background Although several studies assessed the effect of bisphosphonate (BIS) administration on alveolar bone loss, this relationship has not been fully investigated using longitudinal analysis. The aim of the this article is to predict annual alveolar bone loss in a subpopulation of older adults patients who were taking oral bisphosphonate (BIS), adjusting for systemic diseases and associated risk factors. Methods This is a retrospective cohort study. We identified all subjects who reported receiving oral bisphosphonate from 2008 to 2015 (N = 30) using the electronic health records of each patient to identify suitable radiographs for analysis. For the longitudinal data analysis, 26 subjects were eligible for inclusion, having at least two exposures of the complete mouth set or repeated bitewing radiographs at least a one-year interval; they were then matched on age and sex to another 26 patients who did not report receiving bisphosphonate at any point of their life. Results Mild periodontitis was higher in the BIS group compared to the no BIS group; however, moderate periodontitis was higher in the no BIS group. For those who did not take oral BIS, change over time was not significant after the two-year period. However, the BIS group had experienced 0.088 mm more bone loss compared to the no BIS group (95% CI: 0.001, 0.176. P-value = 0.048), adjusting for all other variables included in the model. Conclusion The group that reported receiving oral bisphosphonates showed no improvement in maintaining alveolar bone level, and the use of oral BIS may not be effective in reducing annual alveolar bone loss; however, emerging evidence is promising for the use of bisphosphonate as an adjunctive local delivery medication for the management of periodontal diseases.

scholarly journals Carotid sinus nerve stimulation attenuates alveolar bone loss and inflammation in experimental periodontitis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Aline Barbosa Ribeiro ◽  
Fernanda Brognara ◽  
Josiane Fernandes da Silva ◽  
Jaci Airton Castania ◽  
Patrícia Garani Fernandes ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Bone Loss ◽  
Alveolar Bone ◽  
Carotid Sinus ◽  
Inflammatory Responses ◽  
Periodontal Diseases ◽  
Alveolar Bone Loss ◽  
Carotid Sinus Nerve ◽  
Mandibular Molar ◽  
Inflammatory Processes

Abstract Baroreceptor and chemoreceptor reflexes modulate inflammatory responses. However, whether these reflexes attenuate periodontal diseases has been poorly examined. Thus, the present study determined the effects of electrical activation of the carotid sinus nerve (CSN) in rats with periodontitis. We hypothesized that activation of the baro and chemoreflexes attenuates alveolar bone loss and the associated inflammatory processes. Electrodes were implanted around the CSN, and bilateral ligation of the first mandibular molar was performed to, respectively, stimulate the CNS and induce periodontitis. The CSN was stimulated daily for 10 min, during nine days, in unanesthetized animals. On the eighth day, a catheter was inserted into the left femoral artery and, in the next day, the arterial pressure was recorded. Effectiveness of the CNS electrical stimulation was confirmed by hypotensive responses, which was followed by the collection of a blood sample, gingival tissue, and jaw. Long-term (9 days) electrical stimulation of the CSN attenuated bone loss and the histological damage around the first molar. In addition, the CSN stimulation also reduced the gingival and plasma pro-inflammatory cytokines induced by periodontitis. Thus, CSN stimulation has a protective effect on the development of periodontal disease mitigating alveolar bone loss and inflammatory processes.

Involvement of Cot/Tp12 in Bone Loss during Periodontitis

2010 ◽  
Vol 89 (2) ◽  
pp. 192-197 ◽  
Author(s):  
T. Ohnishi ◽  
A. Okamoto ◽  
K. Kakimoto ◽  
K. Bandow ◽  
N. Chiba ◽  
...  
Keyword(s):  
Bone Loss ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Periodontal Tissue ◽  
Rankl Expression ◽  
Periodontal Tissues ◽  
Tnf Α ◽  
Experimental Periodontitis ◽  
Deficient Mice

Periodontitis causes resorption of alveolar bone, in which RANKL induces osteoclastogenesis. The binding of lipopolysaccharide to Toll-like receptors causes phosphorylation of Cot/Tp12 to activate the MAPK cascade. Previous in vitro studies showed that Cot/Tp12 was essential for the induction of RANKL expression by lipopolysaccharide. In this study, we examined whether Cot/Tp12 deficiency reduced the progression of alveolar bone loss and osteoclastogenesis during experimental periodontitis. We found that the extent of alveolar bone loss and osteoclastogenesis induced by ligature-induced periodontitis was decreased in Cot/Tp12-deficient mice. In addition, reduction of RANKL expression was observed in periodontal tissues of Cot/Tp12-deficient mice with experimental periodontitis. Furthermore, we found that Cot/Tp12 was involved in the induction of TNF-α mRNA expression in gingiva of mice with experimental periodontitis. Our observations suggested that Cot/Tp12 is essential for the progression of alveolar bone loss and osteoclastogenesis in periodontal tissue during experimental periodontitis mediated through increased RANKL expression.

scholarly journals A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1405
Author(s):  
Alfonso Varela-López ◽  
Pedro Bullón ◽  
César L. Ramírez-Tortosa ◽  
María D. Navarro-Hortal ◽  
María Robles-Almazán ◽  
...  
Keyword(s):  
Bone Loss ◽  
Periodontal Disease ◽  
Dietary Habits ◽  
Alveolar Bone ◽  
Periodontal Diseases ◽  
Rabbit Model ◽  
Saturated Fat ◽  
Alveolar Bone Loss ◽  
Standard Diet ◽  
Alcoholic Fatty Liver

Increasing evidence connects periodontitis with a variety of systemic diseases, including metabolic syndrome, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD). The proposal of this study was to evaluate the role of diets rich in saturated fat and cholesterol in some aspects of periodontal diseases in a lipopolysaccharide (LPS)-induced model of periodontal disease in rabbits and to assess the influence of a periodontal intervention on hyperlipidemia, atherosclerosis, and NAFLD progression to non-alcoholic steatohepatitis. Male rabbits were maintained on a commercial standard diet or a diet rich in saturated fat (3% lard w/w) and cholesterol (1.3% w/w) (HFD) for 40 days. Half of the rabbits on each diet were treated 2 days per week with intragingival injections of LPS from Porphyromonas gingivalis. Morphometric analyses revealed that LPS induced higher alveolar bone loss (ABL) around the first premolar in animals receiving standard diets, which was exacerbated by the HFD diet. A higher score of acinar inflammation in the liver and higher blood levels of triglycerides and phospholipids were found in HFD-fed rabbits receiving LPS. These results suggest that certain dietary habits can exacerbate some aspects of periodontitis and that bad periodontal health can contribute to dyslipidemia and promote NAFLD progression, but only under certain conditions.

scholarly journals Features of substitution of defects of tooth rows in persons with periodontal tissue diseases of different severity

2021 ◽  
Vol 105 (1) ◽  
pp. 82-87
Author(s):  
O. Doroshenko ◽  
◽  
O. Bida ◽  
Keyword(s):  
Periodontal Disease ◽  
Bone Tissue ◽  
Dental Implants ◽  
Alveolar Bone ◽  
Periodontal Diseases ◽  
Pathological Process ◽  
Periodontal Tissue ◽  
Dental Implantation ◽  
Orthopedic Rehabilitation ◽  
Abutment Teeth

Resume. The high prevalence of periodontal disease causes the urgency of finding ways to improve methods of diagnosis, treatment and prevention of complications of this disease. The complex clinical picture requires a differentiated approach to the choice of orthopedic rehabilitation of such patients taking into account the patient's age, degree of pathological changes and activity of the pathological process with mandatory immobilization of movable teeth by different types of splints and splint dentures. Depending on the degree of pathological changes in periodontal tissues, the size and topography of dentition defects and the severity of destructive-resorptive processes in the alveolar bone, the replacement of dentition defects should be planned using dental implants and splinttype dentures. The aim of the study. Improving the effectiveness of orthopedic treatment of periodontal diseases, burdened by defects of the dentition through the differentiated use of dental implants and splinting structures. Materials and methods. A comprehensive clinical and laboratory examination of 237 patients with clinically diagnosed periodontitis I–III degree, burdened by partial loss of teeth. From the specified contingent of persons we carried out orthopedic treatment of 98 patients aged from 30 to 69 years with periodontal diseases of various severity, aggravated by defects of dentitions of various size and localization. Cone-beam computed tomography was used for objective quantitative and qualitative assessment of periodontal tissue and bone tissue of the edentulous jaw in individuals with various clinical forms of periodontal disease, to determine the relative densitometric density of bone tissue, when planning dental implantation and for control its results. The localization of supracontacts was determined by occlusion. Diagnosis and elimination of traumatic occlusion were performed according to Jenkelson and Schuller [3]. Evaluation of the stability of dental implants was performed by frequency – resonance analysis of the stability of dental implants using the Osstell ISQ device. Results. According to the results of examination of 237 people and the division of patients into three age groups, the clinical features of the pathological process in the age aspect were established. The results of computed tomography of the dental apparatus of the subjects showed that periodontal disease in the age aspect in the dynamics is accompanied by the severity of destructive-resorptive processes in the alveolar bone, which are manifested in a decrease in the relative densitometric density of bone marrow and bone loss. Orthopedic rehabilitation of this contingent of persons was performed with the use of biologically indifferent structural materials and splinting orthopedic structures, including splinting of abutment teeth, removable prosthetics and dental implantation, as well as accompanying physiotherapy of prostheses. In the case of periodontitis of I–II degree with sufficient volume and density of bone tissue in the area of the dentition defect, high clinical efficacy was shown by the use of dental implantation in the case of gradual loading of the dental implant using a superstructure of own development. In the presence of medium and large defects of the dentition on the background of periodontitis II–III severity in the presence of significant pathological mobility of abutment teeth and insufficient volume and density of bone tissue in the area of the defect sufficient clinical effectiveness showed the use of removable dentures own design. Conclusions. Periodontal diseases in the age aspect in the dynamics are accompanied by pronounced destructive-resorptive processes in the alveolar bone, which are manifested by a decrease in the relative densitometric density of bone tissue and progressive loss of height of the alveolar process. Timely application of dental implantation due to the dosed gradual occlusal load on the alveolar bone helps to preserve the structure of the alveolar bone and the height of the alveolar process. In the presence of medium and large defects of the dentition on the background of periodontitis II–III severity in the presence of significant pathological mobility of abutment teeth and insufficient volume and density of bone tissue in the area of the dentition defect, the use of removable dentures of splinttype design is shown. To prevent complications of prosthetics and prolong the use of orthopedic structures, it is advisable to use photodynamic maintenance therapy. Key words: periodontal tissue disease, dentition defect, dental implantation, splint-type denture.

scholarly journals Effects of smoking on resorption of the residual alveolar ridges in complete denture wearers

2003 ◽  
Vol 56 (9-10) ◽  
pp. 409-412 ◽  
Author(s):  
Dubravka Markovic ◽  
Bojana Jefic ◽  
Duska Blagojevic ◽  
Larisa Blazic
Keyword(s):  
Bone Loss ◽  
Alveolar Bone ◽  
Clinical Investigation ◽  
Periodontal Diseases ◽  
Bacterial Flora ◽  
Anaerobic Bacteria ◽  
Alveolar Bone Loss ◽  
Complete Denture ◽  
Alveolar Ridge ◽  
Oral Epithelial Dysplasia

Introduction Based on literature data it is obvious that there is a connection between smoking and periodontal diseases. Alveolar bone loss increases with smoking. Tobacco smoking affects the proportion of subgingival bacterial flora by influencing oxidoreduction potential of dental plaque and thus making conditions for development of anaerobic bacteria. According to some researchers, smoking affects the mineral component of bone tissue. Orthopantomograms show higher level of alveolar bone loss in smokers than in nonsmokers with the same level of oral hygiene. The aim of this study was to establish if smoking affects alveolar bone loss in complete denture wearers. Material and methods Our clinical investigation included 60 patients of both sexes (30 smokers and 30 nonsmokers) all complete dentures wearers. All patients met study criteria: jaw relation and smokers who smoke over 20 cigarettes per day. All subjects were interviewed, and after that orthopantomograms were made. They were used to calculate the degree of alveolar bone loss. Results The examined subjects were approximately of the same age. Mean age of smokers was 59.9 and nonsmokers 61.8. It was established that differences regarding resorption in men were not significant. The degree of resorption in women smokers and women nonsmokers was different, but differences were not significant. Discussion It has been proven that the number of cigarettes smoked per day is very important. It is considered that the risk of oral epithelial dysplasia increases when smoking more than 20 cigarettes per day. Considering our results regarding resorption of edentulous alveolar ridge in smokers and nonsmokers, we concluded that there were no significant differences. There are opinions in literature that smoking is not an etiological factor in resorption, but there are some opinions that smoking is connected with the degree of resorption in periodontium. The analyses of resorptive changes in edentulous smokers were done only around implants and it was estimated that smoking has more influence than other clinical risk factors. Conclusion On the bases of our research we may conclude that smoking does not directly affect the degree of resorption of edentulous alveolar ridge with complete denture wearers.

scholarly journals Association between Periodontal Disease and Comorbidities in Saudi’s Eastern Province

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Marwa Madi ◽  
Hatem M. Abuohashish ◽  
Dina Attia ◽  
Norah AlQahtani ◽  
Nabras Alrayes ◽  
...  
Keyword(s):  
Bone Loss ◽  
Periodontal Disease ◽  
Alveolar Bone ◽  
Periodontal Diseases ◽  
Demographic Data ◽  
Alveolar Bone Loss ◽  
Haemoglobin A1c ◽  
Eastern Province ◽  
Pocket Depth ◽  
Multivariable Regression Models

The incidence of periodontal diseases is associated with multiple comorbidities that influence a patient’s treatment planning. This study evaluates the relation between periodontal disease and multiple comorbidities reported in the Saudi population from the Eastern province. This study was conducted on 190 patients, who visited the periodontology clinics at Imam Abdulrahman Bin Faisal University, Saudi Arabia. Demographic data, smoking habits, past medical and dental histories, blood pressure, random blood glucose, and recent haemoglobin A1c were recorded. A comprehensive periodontal examination included the number of missing teeth, pocket depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), and mobility of all teeth except third molars. Radiographic bone loss was measured on standardized full-mouth periapical radiographs. Multivariable regression models were calculated aiming to see the association between different comorbidities and alveolar bone loss with confounders controlled. Out of 190 periodontitis patients, 56 (29.5%) were males and 134 (70.5%) were females. More than half of the patients (60%) were between 26 and 50 years, 30% of them had diabetes, and 18% were smokers. The risk of alveolar bone loss was higher in persons who had diabetes and those who had both diabetes and coronary heart disease than those who did not, although the association was not statistically significant ( B = 1.26 , 95 % CI = − 0.30 , 2.82, and B = 2.86 , 95 % CI = − 1.25 , 6.96, respectively). The risk of alveolar bone loss was significantly higher among persons with diabetes and hypertension ( B = 2.82 and 95 % CI = 0.89 , 4.75). Collectively, the risk of alveolar bone loss in periodontitis patients increases with diabetes in the presence of other comorbidities regardless of smoking or gender.

scholarly journals Annual Alveolar Bone Loss in Older Adults Taking Oral Bisphosphonate: a Case-Control Study

2019 ◽  
Author(s):  
Mohammad Helmi ◽  
Sara AlOsaimy ◽  
J. Max Goodson ◽  
Hatice Hasturk ◽  
Zuhair S. Natto
Keyword(s):  
Older Adults ◽  
Bone Loss ◽  
Case Control Study ◽  
Alveolar Bone ◽  
Periodontal Diseases ◽  
Case Control ◽  
Alveolar Bone Loss ◽  
P Value ◽  
Oral Bisphosphonate ◽  
Control Study

Abstract Background Although several studies assessed the effect of bisphosphonate (BIS) administration on alveolar bone loss, this relationship using longitudinal analysis has not been fully investigated. The aim of the this article is to predict annual alveolar bone loss in a subpopulation of older adults patients who were taking oral bisphosphonate (BIS) adjusting for systemic diseases and associated risk factors. Methods This is a case-control study. We identified all subjects that reported receiving oral bisphosphonate from 2008 – 2015 (N=30) using the electronic health records of each patient to identify suitable radiographs for analysis. For longitudinal data analysis, 26 eligible subjects for inclusion to have at least two exposures of complete mouth set or repeated bitewing radiographs with at least one-year interval, then matched on age and sex to another 26 patients who did not report receiving bisphosphonate at any point of their life. Results Mild periodontitis was higher in the BIS group compared to the no BIS group; however, moderate periodontitis was higher in the no BIS group. For those who did not take oral BIS, change over time was not significant after the two-year period. However, BIS group had experienced 0.088 mm more bone loss compared to no BIS group (95% CI: 0.001, 0.176. P-value = 0.048), adjusting for all other variables included in the model. Conclusion The group who reported receiving oral bisphosphonates showed no improvement in maintaining alveolar bone level, and the use of oral BIS may not be effective in reducing annual alveolar bone loss, however, emerging evidence is promising for the use of bisphosphonate as an adjunctive local delivery medication for management of periodontal diseases.

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