scholarly journals The Role of Substrate Topography and Stiffness on MSC Cells Functions: Key Material Properties for Biomimetic Bone Tissue Engineering

Biomimetics ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 7
Author(s):  
Foteini K. Kozaniti ◽  
Despina D. Deligianni ◽  
Margarita D. Georgiou ◽  
Diana V. Portan
Keyword(s):  
Bone Cells ◽  
Deep Understanding ◽  
Solvent Casting ◽  
Key Factors ◽  
Substrate Topography ◽  
Superior Mechanical Properties ◽  
And Migration ◽  
Moduli Of Elasticity ◽  
The Ideal

The hypothesis of the present research is that by altering the substrate topography and/or stiffness to make it biomimetic, we can modulate cells behavior. Substrates with similar surface chemistry and varying stiffnesses and topographies were prepared. Bulk PCL and CNTs-reinforced PCL composites were manufactured by solvent casting method and electrospinning and further processed to obtain tunable moduli of elasticity in the range of few MPa. To ensure the same chemical profile for the substrates, a protein coating was added. Substrate topography and properties were investigated. Further on, the feedback of Wharton’s Jelly Umbilical Cord Mesenchymal Stem Cells to substrates characteristics was investigated. Solvent casting scaffolds displayed superior mechanical properties compared to the corresponding electrospun films. However, the biomimetic fibrous texture of the electrospun substrates induced improved feedback of the cells with respect to their viability and proliferation. Cells’ adhesion and differentiation was remarkably pronounced on solvent casting substrates compared to the electrospun substrates. Soft substates improved cells multiplication and migration, while stiff substrates induced differentiation into bone cells. Aspects related to the key factors and the ideal properties of substrates and microenvironments were clarified, aiming towards the deep understanding of the required optimum biomimetic features of biomaterials.

scholarly journals The Role of International Cooperation in Demarcation of Territorial Boundaries in International Law and Procedures

2016 ◽  
Vol 10 (9) ◽  
pp. 81
Author(s):  
Mohammad Saberrad ◽  
Seyed Ghasem Zamani
Keyword(s):  
International Law ◽  
International Cooperation ◽  
International Organizations ◽  
International Institutions ◽  
Deep Understanding ◽  
International Peace ◽  
Boundary Issues ◽  
Territorial Boundaries ◽  
The Ideal

Non-judicial developments in the field of boundary issues, with the aim of achieving stability and preserve it, move toward the ideal of international community. The ideal is to maintain international peace, order and security. Developments and advances are in the service of this ideal and countries take steps in this direction with responsibility than before and with believe in integrity of international peace, order and security, and apply technical and human progresses in this way. International organizations and countries, as the main subjects of international law, with understanding adverse consequences of instability, use all the tools necessary to achieve this goal and non-judicial developments occurring in delimitation of international territorial boundaries, are in line with this excellent goal. Today, countries cooperate wider than past in different ways and in all fields including boundary issues. Deep understanding toward the importance of international peace, order and security and legal belief to necessity of such cooperation, undoubtedly have facilitate today direction. Of course, the role of international institutions and organizations was very dramatic in developing such belief.

Credit constraints and Rural Migration: Evidence from Six Villages in Uttar Pradesh

2018 ◽  
Vol 15 (3) ◽  
pp. 389-398
Author(s):  
Ruchi Singh
Keyword(s):  
Credit Constraints ◽  
Uttar Pradesh ◽  
Binary Logistic Regression ◽  
Binary Logistic Regression Model ◽  
Male Migration ◽  
Rural Economies ◽  
And Migration ◽  
The Relationship ◽  
The Empirical Analysis

Rural economies in developing countries are often characterized by credit constraints. Although few attempts have been made to understand the trends and patterns of male out-migration from Uttar Pradesh (UP), there is dearth of literature on the linkage between credit accessibility and male migration in rural Uttar Pradesh. The present study tries to fill this gap. The objective of this study is to assess the role of credit accessibility in determining rural male migration. A primary survey of 370 households was conducted in six villages of Jaunpur district in Uttar Pradesh. Simple statistical tools and a binary logistic regression model were used for analyzing the data. The result of the empirical analysis shows that various sources of credit and accessibility to them play a very important role in male migration in rural Uttar Pradesh. The study also found that the relationship between credit constraints and migration varies across various social groups in UP.

They think they find themselves: radical violence and narcissistic-identity suffering

2019 ◽  
Vol 1 (1) ◽  
pp. 21-31 ◽  
Author(s):  
Louis Brunet
Keyword(s):  
Lone Wolf ◽  
The Creation ◽  
Ideal Ego ◽  
Group Regression ◽  
The Ideal

This article proposes a model of individual violent radicalisation leading to acts of terrorism. After reviewing the role of group regression and the creation of group psychic apparatus, the article will examine how violent radicalisation, by the reversal of the importance of the superego and the ideal ego, serves to compensate the narcissistic identity suffering by “lone wolf” terrorists.

Role of microenvironment on proliferation and migration of an SDHB silenced murine Pheochromocytoma cell line

2017 ◽  
Author(s):  
Serena Martinelli ◽  
Vanessa D'Antongiovanni ◽  
Susan Richter ◽  
Letizia Canu ◽  
Tonino Ercolino ◽  
...  
Keyword(s):  
Cell Line ◽  
Pheochromocytoma Cell ◽  
And Migration ◽  
Proliferation And Migration

Glioblastoma invasion and NMDA receptors: A novel prospect

2019 ◽  
Vol 106 (3) ◽  
pp. 250-260 ◽  
Author(s):  
DN Nandakumar ◽  
P Ramaswamy ◽  
C Prasad ◽  
D Srinivas ◽  
K Goswami
Keyword(s):  
Glutamate Receptors ◽  
Cell Lines ◽  
Intracellular Signaling ◽  
Transcript Level ◽  
Glioblastoma Cells ◽  
Invasion And Migration ◽  
Matrigel Invasion ◽  
Nmdar Activation ◽  
And Migration

Purpose Glioblastoma cells create glutamate-rich tumor microenvironment, which initiates activation of ion channels and modulates downstream intracellular signaling. N-methyl-D-aspartate receptors (NMDARs; a type of glutamate receptors) have a high affinity for glutamate. The role of NMDAR activation on invasion of glioblastoma cells and the crosstalk with α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) is yet to be explored. Main methods LN18, U251MG, and patient-derived glioblastoma cells were stimulated with NMDA to activate NMDAR glutamate receptors. The role of NMDAR activation on invasion and migration and its crosstalk with AMPAR were evaluated. Invasion and migration of glioblastoma cells were investigated by in vitro trans-well Matrigel invasion and trans-well migration assays, respectively. Expression of NMDARs and AMPARs at transcript level was evaluated by quantitative real-time polymerase chain reaction. Results We determined that NMDA stimulation leads to enhanced invasion in LN18, U251MG, and patient-derived glioblastoma cells, whereas inhibition of NMDAR using MK-801, a non-competitive antagonist of the NMDAR, significantly decreased the invasive capacity. Concordant with these findings, migration was significantly augmented by NMDAR in both cell lines. Furthermore, NMDA stimulation upregulated the expression of GluN2 and GluA1 subunits at the transcript level. Conclusions This study demonstrated the previously unexplored role of NMDAR in invasion of glioblastoma cells. Furthermore, the expression of the GluN2 subunit of NMDAR and the differential overexpression of the GluA1 subunit of AMPAR in both cell lines provide a plausible rationale of crosstalk between these calcium-permeable subunits in the glutamate-rich microenvironment of glioblastoma.

TRIM44 promotes cell proliferation and migration by inhibiting FRK in renal cell carcinoma

2020 ◽  
Author(s):  
Lungwani Muungo
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Proliferation ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Lymphatic Invasion ◽  
Cancer Specific Survival ◽  
Candidate Target ◽  
Candidate Target Gene ◽  
And Migration

TRIM44 has oncogenic roles in various cancers. However, TRIM44 expression andits function in renal cell carcinoma (RCC) are still unknown. Here in this study, weinvestigated the clinical significance of TRIM44 and its biological function in RCC.TRIM44 overexpression was significantly associated with clinical M stage, histologictype (clear cell) and presence of lymphatic invasion (P = .047, P = .005, and P = .028,respectively). Moreover, TRIM44 overexpression was significantly associated withpoor prognosis in terms of cancer-specific survival (P = .019). Gain-of-function andloss-of-function studies using TRIM44 and siTRIM44 transfection showed thatTRIM44 promotes cell proliferation and cell migration in two RCC cell lines, Caki1and 769P. To further investigate the role of TRIM44 in RCC, we performed integratedmicroarray analysis in Caki1 and 769P cells and explored the data in the Oncominedatabase. Interestingly, FRK was identified as a promising candidate target gene ofTRIM44, which was downregulated in RCC compared with normal renal tissues. Wefound that cell proliferation was inhibited by TRIM44 knockdown and then recoveredby siFRK treatment. Taken together, the present study revealed the associationbetween high expression of TRIM44 and poor prognosis in

Mereka yang Tidak Dibayar Tinggi: Ibuisme, Taman Kanak-kanak, dan Kampung di Indramayu

2018 ◽  
Vol 3 (2) ◽  
pp. 121
Author(s):  
Mochammad Arief Wicaksono
Keyword(s):  
Kindergarten Teachers ◽  
New Order ◽  
The Family ◽  
Role Of Women ◽  
Community Pressure ◽  
The Village ◽  
Depth Interviews ◽  
The Ideal

The ideology of state-ibuism has always been interwoven with how the New Order regime until nowadays government constructing the “ideal” role of women in the family and community through the PKK (Pembinaan Kesejahteraan Keluarga) organization. However, in Cangkring Village, Indramayu, the ideology of ibuism works not because of the massive government regulating the role of women through the PKK organization, but it is possible because of the structure of the kampung community itself. Through involved observations and in-depth interviews about a kindergarten in the village, a group of housewives who dedicated themselves to teaching in kindergarten were met without getting paid high. From these socio-cultural phenomenons, this paper will describe descriptively and analytically that housewives in the Cangkring village are willing to become kindergarten teachers because of their moral burden as part of the warga kampung and also from community pressure from people who want their children to be able to read and write.

Ч. АЙТМАТОВДУН ЧЫГАРМАЛАРЫНДАГЫ ЖАЛПЫ АДАМЗАТТЫК БААЛУУЛУКТАРДЫН МААНИСИ ЖАНА ЖАШ МУУНДАРДЫ ТАРБИЯЛООДОГУ ОРДУ

2020 ◽  
pp. 164-167
Author(s):  
Айнура Байтокова
Keyword(s):  
Deep Understanding ◽  
Cultural Development ◽  
Point Of View ◽  
Human Values ◽  
Spiritual Values ◽  
Future Teachers ◽  
Youth Education ◽  
Spiritual World ◽  
Universal Values

Аннотация. Макала учурдагы актуалдуу проблемалардын бири болгон жаш муундарда жалпы адамзаттык баалуулуктарды тарбиялоо. Макалада Ч. Айтматовдун чыгармаларындагы баалуулуктардын мааниси жана алардын жаш муундарды тарбиялоодогу орду жөнүндө маселени козгойт. Өзгөчө жаңы социалдык экономикалык өзгөрүүлөр менен жаш муундардын адеп-ахлактык, руханий дүйнөсү өзгөрүүдө. Бул жагдайдан алып караганда коомдун социалдык-маданий жактан андан ары өнүгүүсү үчүн башкы бириктирүүчү факторлордон болуп жаш муундардагы жалпы адамзаттык баалуулуктарды калыптандыруу маселеси биринчи орунга чыгат. Болочок мугалимдердин кесиптик билим алуусунда жалпы адамзаттык баалуулуктарды терең түшүнүүсү, алардын келечектеги кесиптик даярдыгына жардам берет. Демек, аларга жалпы адамзаттык баалуулуктарды сиңирүү абдан маанилүү учур талабы экендигин көрөбүз. Түйүндүү сөздөр: жалпы адамзаттык баалуулуктар, адамгерчилик, адеп-ахлак, руханий баалуулуктар, учурдагы көйгөйлүү маселелер, жаш муундар, базар экономикасы ж.б... Аннотация. В статье затронута одна из актуальных проблем современности – достойное воспитание молодого поколения. Статья рассматривает проблематику воспитания молодежи и формирования общечеловеческих ценностей. В статье речь идет о роли произведений Чингиза Айтматова в воспитании молодежи. В условиях перехода в новый социально-экономический период меняется нравственно-духовный мир молодежи, в том числе у студентов. С этой точки зрения на первый план выходит задача формирования общечеловеческих ценностей, которые должны стать главным объединяющим фактором дальнейшего социокультурного развития общества. Обогащение опыта глубокого понимания общечеловеческих ценностей у будущих педагогов в профессиональном обучении способствует формированию подготовленности будущих педагогов профессионального обучения в будущем, также важно привить в них систему общечеловеческих ценностей. Ключевые слова: общечеловеческие ценности, гуманность, нравственно-этические принципы, духовные ценности, рыночная экономика. Annotation. the article touches on one of the pressing problems of our time - the decent education of the younger generation. The article considers the issues of youth education and the formation of universal values. The article deals with the role of the works of Chingiz Aitmatov in the education of youth. With the transition to a new socio- economic period, the moral and spiritual world of young people is changing, including among students. From this point of view, the task of creating universal human values comes to the forefront, which should become the main unifying factor in the further socio-cultural development of society. The enrichment of the experience of a deep understanding of human values in future teachers contributes to the formation of preparedness of future teachers of vocational training in the future, it is also important to instill in them a system of universal values. Keywords: universal values, humanity, thinker, wise, moral and ethical principles, spiritual values, market economy.

PTTG has a Dual Role of Promotion-Inhibition in the Development of Pituitary Adenomas

2019 ◽  
Vol 26 (11) ◽  
pp. 800-818
Author(s):  
Zujian Xiong ◽  
Xuejun Li ◽  
Qi Yang
Keyword(s):  
Cell Cycle ◽  
Pituitary Adenoma ◽  
Pituitary Adenomas ◽  
Cell Cycle Progression ◽  
Key Factors ◽  
Cycle Progression ◽  
Sister Chromatids ◽  
Regulation Of Cell Cycle ◽  
Late Stages

Pituitary Tumor Transforming Gene (PTTG) of human is known as a checkpoint gene in the middle and late stages of mitosis, and is also a proto-oncogene that promotes cell cycle progression. In the nucleus, PTTG works as securin in controlling the mid-term segregation of sister chromatids. Overexpression of PTTG, entering the nucleus with the help of PBF in pituitary adenomas, participates in the regulation of cell cycle, interferes with DNA repair, induces genetic instability, transactivates FGF-2 and VEGF and promotes angiogenesis and tumor invasion. Simultaneously, overexpression of PTTG induces tumor cell senescence through the DNA damage pathway, making pituitary adenoma possessing the potential self-limiting ability. To elucidate the mechanism of PTTG in the regulation of pituitary adenomas, we focus on both the positive and negative function of PTTG and find out key factors interacted with PTTG in pituitary adenomas. Furthermore, we discuss other possible mechanisms correlate with PTTG in pituitary adenoma initiation and development and the potential value of PTTG in clinical treatment.

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