scholarly journals Tissue Characterization Using an Electrical Bioimpedance Spectroscopy-Based Multi-Electrode Probe to Screen for Cervical Intraepithelial Neoplasia

Diagnostics ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2354
Author(s):  
Tong In Oh ◽  
Min Ji Kang ◽  
You Jeong Jung ◽  
Tingting Zhang ◽  
Seung Geun Yeo ◽  
...  
Keyword(s):  
Electrical Properties ◽  
Cervical Intraepithelial Neoplasia ◽  
Screening Method ◽  
Intraepithelial Neoplasia ◽  
Energy Concentration ◽  
Bioimpedance Spectroscopy ◽  
Frequency Spectra ◽  
Measurement Protocol ◽  
Electrical Bioimpedance ◽  
The Difference

The successful management of cervical intraepithelial neoplasia (CIN) with proper screening and treatment methods could prevent cervical cancer progression. We propose a bioimpedance spectroscopic measurement device and a multi-electrode probe as an independent screening tool for CIN. To evaluate the performance of this screening method, we enrolled 123 patients, including 69 patients with suspected CIN and 54 control patients without cervical dysplasia who underwent a hysterectomy for benign disease (non-CIN). Following conization, the electrical properties of the excised cervical tissue were characterized using an electrical bioimpedance spectroscopy-based multi-electrode probe. Twenty-eight multifrequency voltages were collected through the two concentric array electrodes via a sensitivity-optimized measurement protocol based on an electrical energy concentration method. The electrical properties of the CIN and non-CIN groups were compared with the results of the pathology reports. Reconstructed resistivity tended to decrease in the CIN and non-CIN groups as frequency increased. Reconstructed resistivity from 625 Hz to 50 kHz differed significantly between the CIN and non-CIN groups (p < 0.001). Using 100 kHz as the reference, the difference between the CIN and non-CIN groups was significant. Based on the difference in reconstructed resistivity between 100 kHz and the other frequencies, this method had a sensitivity of 94.3%, a specificity of 84%, and an accuracy of 90% in CIN screening. The feasibility of noninvasive CIN screening was confirmed through the difference in the frequency spectra evaluated in the excised tissue using the electrical bioimpedance spectroscopy-based multi-electrode screening probe.

Current Source Design for Electrical Bioimpedance Spectroscopy

2008 ◽  
pp. 359-367 ◽  
Author(s):  
Fernando Seoane ◽  
Ramón Bragos ◽  
Kaj Lindecrantz ◽  
Pere Riu
Keyword(s):  
Electrical Properties ◽  
Impedance Spectroscopy ◽  
Biological Tissue ◽  
Electrical Impedance ◽  
Current Source ◽  
Electrical Impedance Spectroscopy ◽  
Bioimpedance Spectroscopy ◽  
Electrical Bioimpedance ◽  
Electronic Instrumentation ◽  
Passive Electrical Properties

The passive electrical properties of biological tissue have been studied since the 1920s, and with time, the use of Electrical Bioimpedance (EBI) in medicine has successfully spread (Schwan, 1999). Since the electrical properties of tissue are frequency-dependent (Schwan, 1957), observations of the bioimpedance spectrum have created the discipline of Electrical Impedance Spectroscopy (EIS), a discipline that has experienced a development closely related to the progress of electronic instrumentation and the dissemination of EBI technology through medicine.

scholarly journals Association of Human Papillomavirus 31 DNA Load with Risk of Cervical Intraepithelial Neoplasia Grades 2 and 3

2015 ◽  
Vol 53 (11) ◽  
pp. 3451-3457 ◽  
Author(s):  
Xia Liu ◽  
Mark Schiffman ◽  
Ayaka Hulbert ◽  
Zhonghu He ◽  
Zhenping Shen ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Cervical Intraepithelial Neoplasia ◽  
Intraepithelial Neoplasia ◽  
Low Grade ◽  
Random Set ◽  
Squamous Cells ◽  
Viral Loads ◽  
Unit Increase ◽  
Intraepithelial Lesion ◽  
The Difference

The association between human papillomavirus 31 (HPV31) DNA loads and the risk of cervical intraepithelial neoplasia grades 2 and 3 (CIN2–3) was evaluated among women enrolled in the atypical squamous cells of undetermined significance (ASCUS) and low-grade squamous intraepithelial lesion (LSIL) triage study (ALTS), who were monitored semiannually over 2 years and who had HPV31 infections detected at ≥1 visit. HPV31 DNA loads in the first HPV31-positive samples and in a random set of the last positive samples from women with ≥2 HPV31-positive visits were measured by a real-time PCR assay. CIN2–3 was histologically confirmed at the same time as the first detection of HPV31 for 88 (16.6%) of 530 women. After adjustment for HPV31 lineages, coinfection with other oncogenic types, and the timing of the first positive detection, the odds ratio (OR) per 1-log-unit increase in viral loads for the risk of a concurrent diagnosis of CIN2–3 was 1.5 (95% confidence interval [CI], 1.2 to 1.9). Of 373 women without CIN2–3 at the first positive visit who had ≥1 later visit, 44 had subsequent diagnoses of CIN2–3. The initial viral loads were associated with CIN2–3 diagnosed within 6 months after the first positive visit (adjusted OR, 1.5 [95% CI, 1.0 to 2.4]) but were unrelated to CIN2–3 diagnosed later. For a random set of 49 women who were tested for viral loads at the first and last positive visits, changes in viral loads were upward and downward among women with and without follow-up CIN2–3 diagnoses, respectively, although the difference was not statistically significant. Results suggest that HPV31 DNA load levels at the first positive visit signal a short-term but not long-term risk of CIN2–3.

Validation of a low-cost, liquid-based screening method for cervical intraepithelial neoplasia

2006 ◽  
Vol 195 (4) ◽  
pp. 965-970 ◽  
Author(s):  
Judy M. Lee ◽  
Deidra Kelly ◽  
Patti E. Gravitt ◽  
Zoya Fansler ◽  
John A. Maksem ◽  
...  
Keyword(s):  
Cervical Intraepithelial Neoplasia ◽  
Low Cost ◽  
Screening Method ◽  
Intraepithelial Neoplasia

scholarly journals Triage by PAX1 and ZNF582 methylation in women with cervical intraepithelial neoplasia grade 3: a multicenter case-control study

2022 ◽  
Author(s):  
Kun Fu ◽  
Ming Lei ◽  
Li-Sha Wu ◽  
Jing-Cheng Shi ◽  
Si-Yu Yang ◽  
...  
Keyword(s):  
Cervical Intraepithelial Neoplasia ◽  
Predictive Accuracy ◽  
Screening Method ◽  
Intraepithelial Neoplasia ◽  
Fold Increase ◽  
Low Grade ◽  
Squamous Intraepithelial Lesion ◽  
Cervical Intraepithelial Neoplasia Grade ◽  
Intraepithelial Lesion ◽  
Grade 3

Abstract Background The colposcopy-conization inconsistency is common in women with cervical intraepithelial neoplasia grade 3 (CIN3). No adequate method has been reported to identify the final pathology of conization. In this study, we explored the ability of PAX1 and ZNF582 methylation to predict the pathological outcome of conization in advance. Methods This was a multicenter study and included 277 histologically confirmed CIN3 women who underwent cold knife conization (CKC) from January 2019 to December 2020. The methylation levels of PAX1 (PAX1m) and ZNF582 (ZNF582 m) were determined by quantitative methylation specific PCR (qMSP) and expressed in ΔCp. Receiver-operating characteristic (ROC) curves were used to evaluate predictive accuracy. Results The final pathological results in 48 (17.33%) patients were inflammation or low-grade squamous intraepithelial lesion (LSIL), 190 (68.59%) were high grade squamous intraepithelial lesion (HSIL) and 39 (14.08%) were squamous cervical cancer (SCC). PAX1 m and ZNF582 m increased as lesions progressed from inflammation/LSIL, HSIL to SCC. PAX1 and ZNF582 methylation yielded better prediction performance compared to common screening strategies, whether individually or combined. ΔCpZNF582 ≥19.18). A 6.53-fold increase in SCC risk was observed in patients with elevated ZNF582 methylation (ΔCpZNF582 &lt; 7.09). Conclusion DNA Methylation would be an alternative screening method to triage and predict the final outcome of conization of the CIN3 cases.

scholarly journals Correlation between ER, PR, P53, Ki67 expression, and high-risk HPV infection in patients with different levels of cervical intraepithelial neoplasia

2020 ◽  
Vol 18 ◽  
pp. 205873922093308
Author(s):  
Gao Yuan
Keyword(s):  
High Risk ◽  
Correlation Analysis ◽  
Cervical Intraepithelial Neoplasia ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Ki67 Expression ◽  
Positive Correlation ◽  
Positive Rate ◽  
The Difference ◽  
High Risk Hpv

This study was designed to investigate the correlation between high-risk human papillomavirus (HPV) infection and the expression of IHC markers (ER, PR, p53, Ki67) in patients with different grades of cervical intraepithelial neoplasia (CIN). It was a retrospective study, which was conducted from June 2016 to June 2018. 140 specimens of CIN were collected from the pathology department of a certain hospital that included 40 specimens of CIN1, 50 specimens of CIN2 and 50 specimens of CIN3. The expression of ER, PR, P53 and Ki67 were determined by immunohistochemistry. The high-risk HPV infections were detected by PCR fluorescence quantification and were given the correlation analysis. In the 140 specimens, the positive rates of HPV16 and HPV18 in CIN1 specimens were 27.5% and 25.0% respectively, and in CIN2 specimens were 64.0% and 60.0% respectively, and in CIN3 specimens were 90.0% and 92.0% respectively, the difference were statistically significant (p<0.05). There were no significant correlation (p<0.05) between HPV16 and HPV18 positive rate and patient age, tissue differentiation, and tumor size. With the increased of CIN grade, the positive rate of ER, PR, P53 and Ki67 expression in specimen were also increased significantly, and the difference were statistically significant (p<0.05). Pearson correlation analysis showed there were positive correlation (p<0.05) between the positive rates of HPV16 and HPV18 and the positive rates of ER, PR, P53 and Ki67. With the increase of CIN level, the positive rates of high-risk HPV infection as well as ER, PR, P53 and Ki67 are increased, and they have positive correlation.

scholarly journals Detection of cervical intraepithelial neoplasia (CIN) by visual inspection of cervix with acetic acid (VIA) and its comparison to cervical cytology

2019 ◽  
Vol 31 (1) ◽  
pp. 15-20
Author(s):  
Nargis Zahan ◽  
Mosammat Nargis Shamima ◽  
Sharmin Sultana ◽  
Mohd Alamgir Hossain
Keyword(s):  
Cervical Cancer ◽  
Acetic Acid ◽  
Cervical Intraepithelial Neoplasia ◽  
Visual Inspection ◽  
Pap Smear ◽  
Early Stage ◽  
Invasive Cervical Cancer ◽  
Screening Method ◽  
Intraepithelial Neoplasia ◽  
Screening Tools

Background: Cervical cancer is the second most common cancer in women throughout the world, and it is the leading cause of cancer death among women in underdeveloped countries like Bangladesh is preventable and curable if detected at and early stage using proper screening tools. This study was done to see the effectiveness of VIA and find out the CIN and introduce as a complementary to cytology for diagnosing precancerous form of cervix. Materials & Methods: A total 175 subjects were studied & relevant data of cervix related patients have been collected. The data regarding Pap smear, VIA and biopsy have been taken and collected data was analysis by SPSS. Results: Out of 175 Patients VIA positive 53 (30.3%), Pap smear reports, 84 (48.0%) had inflammatory findings and 38 (21.7%) had dysplasia and biopsy result 41(23.4%) CIN positive. Sensitivity of VlA was 90.2%, specificity 88.1%, PPV 69.8%, NPV 96.7% and accuracy 88.6%. Sensitivity of Pap smear reports was 80.5%, specificity 96.3%, PPV 86.8%, NPV 94.2% and accuracy 92.6%. Conclusion: Visual inspection of cervix after application of acetic acid (VIA) is valid as cytology test for the identification of pre-invasive cervical cancer (CIN). Thus VIA is a useful screening method of Cervical Intraepithelial Neoplasia lesion as Pap smear. TAJ 2018; 31(1): 15-20

scholarly journals Performance Evaluation of Multiple Electrodes Based Electrical Impedance Spectroscopic Probe for Screening of Cervical Intraepithelial Neoplasia

Electronics ◽  
2021 ◽  
Vol 10 (16) ◽  
pp. 1933
Author(s):  
Tingting Zhang ◽  
Youjeong Jeong ◽  
Dongchoon Park ◽  
Tongin Oh
Keyword(s):  
Electrical Properties ◽  
Impedance Spectroscopy ◽  
Cervical Intraepithelial Neoplasia ◽  
Electrical Impedance ◽  
Low Cost ◽  
High Sensitivity ◽  
Intraepithelial Neoplasia ◽  
Electrical Impedance Spectroscopy ◽  
Voltage Measurement ◽  
Wide Range

As regular cervical cancer screening becomes more common, the detection of cervical intraepithelial neoplasia (CIN) is increasing. We proposed a noninvasive and low-cost multi-channel electrical impedance spectroscopy (EIS) and probe with multiple active electrodes for screening CIN. Compared with four-electrode probes for impedance spectroscopy, the multiple active electrodes facilitated more flexible combination of current injection and voltage measurement, which allowed well-designed measurement protocols for focused sensitivity underneath the large size of the probe. Furthermore, the multiple active electrodes reduced the negative effects of the cabling between the system and probe inserted into the cervix. After presenting the basic performance, the EIS probe was tested by three different experimental phantoms using four different materials of electrical properties. The corresponding experimental results were presented to prove the functionality of the EIS probe and characterize the electrical properties at a wide range of frequencies from 0.625 to 100 kHz. It had high sensitivity underneath the surface of the probe and a rapidly decreased outer. Furthermore, we verified the frequency-dependent impedance changes using the giant vesicle phantoms with different amounts of extra- and intra-fluids separated by the insulating membranes. This study facilitates the feasibility into clinical practice for identifying CIN in the future.

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