scholarly journals Effects of Exogenous Ethylene and Cobalt Chloride on Root Growth of Chinese Fir Seedlings under Phosphorus-Deficient Conditions

Forests ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1585
Author(s):  
Hui Zhang ◽  
Nemin Wang ◽  
Shanshan Zheng ◽  
Min Chen ◽  
Xiangqing Ma ◽  
...  
Keyword(s):  
Cobalt Chloride ◽  
Chinese Fir ◽  
Physiological Changes ◽  
P Deficiency ◽  
Adaptive Mechanisms ◽  
Malondialdehyde Content ◽  
Ethephon Treatment ◽  
Exogenous Ethylene ◽  
P Supply ◽  
Area And Volume

Studying the effects of different concentrations of ethephon on morphological and physiological changes in the roots of Chinese fir (Cunninghamia lanceolata Lamb. Hook.) seedlings under P deficiency can reveal the internal adaptive mechanisms of these plants under nutrient stress. Herein, we investigated the effects of different ethephon and cobalt chloride concentrations under normal P supply and P deficiency. A significant effect (p < 0.05) of exogenous additive application was observed on the development of Chinese fir root length, surface area, and volume. These root development indices showed maximum values when the ethephon concentration was 0.01 g kg−1 under normal P supply and P deficiency, and they were significantly different from those under 0.04 g kg−1 ethephon treatment. Similarly, the indices showed maximum values when CoCl2 concentration was 0.01 g kg−1 under P deficiency and was significantly different (p < 0.01) from those under 0.2 g kg−1 CoCl2 treatment. Under normal P supply, an increase in ethephon concentration caused superoxide dismutase (SOD; E.C. 1.15.1.1) activity to decrease and peroxidase (POD; E.C. 1.11.1.X) activity to increase gradually. Conversely, CoCl2 addition (0.01 g kg−1) promoted SOD and POD activities under P deficiency. There were no significant differences (p > 0.05) in malondialdehyde content of seedlings among ethephon or CoCl2 treatments. In conclusion, ethylene plays a significant role in adaptative mechanisms underlying stress resistance in plants, prompting them to respond to P starvation and improving seedlings’ tolerance to P-deficient conditions.

Root morphological plasticity and biomass production of two Chinese fir clones with high phosphorus efficiency under low phosphorus stress

2011 ◽  
Vol 41 (2) ◽  
pp. 228-234 ◽  
Author(s):  
Pengfei Wu ◽  
Xiangqing Ma ◽  
Mulualem Tigabu ◽  
Chen Wang ◽  
Aiqin Liu ◽  
...  
Keyword(s):  
Biomass Production ◽  
Morphological Traits ◽  
Southern China ◽  
Chinese Fir ◽  
Phosphorus Efficiency ◽  
Sand Culture ◽  
Root Surface Area ◽  
Limiting Factor ◽  
P Deficiency ◽  
P Supply

Available P is the major growth-limiting factor in southern China where Chinese fir ( Cunninghamia lanceolata (Lamb.) Hook.) plantations are increasingly established. Planting P-efficient clones is a viable option to enhance productivity of Chinese fir plantations. Two Chinese fir clones with high P efficiency (M1 and M4) were chosen as the research materials and their adaptive responses to low P stress were examined. The increment in root morphological traits and biomass production of these clones was measured by a sand culture experiment with a heterogeneous P supply. For both clones, P starvation resulted in significantly higher root surface area and root volume but not root length. For clone M4, the mean root diameter was also larger under P deficiency than under normal P supply. Interestingly, the root morphological traits varied substantially within the same root system where the starved roots had higher values for all morphological traits than the nonstarved ones. Phosphorus starvation did not affect shoot and root biomass or the root to shoot ratio, but the whole-plant biomass increment was large under P deficiency for clone M4. In conclusion, the adaptation to low P stress in these clones is attributed to increased P acquisition and utilization efficiencies.

Sex-specifically responsive strategies to phosphorus availability combined with different soil nitrogen forms in dioecious Populus cathayana

2021 ◽  
Author(s):  
Xiucheng Liu ◽  
Yuting Wang ◽  
Shuangri Liu ◽  
Miao Liu
Keyword(s):  
Root Length ◽  
Root Length Density ◽  
Specific Root Length ◽  
P Uptake ◽  
Root Tip ◽  
P Availability ◽  
P Deficiency ◽  
Populus Cathayana ◽  
Leaf P ◽  
P Supply

Abstract Aims Phosphorus (P) availability and efficiency are especially important for plant growth and productivity. However, the sex-specific P acquisition and utilization strategies of dioecious plant species under different N forms are not clear. Methods This study investigated the responsive mechanisms of dioecious Populus cathayana females and males based on P uptake and allocation to soil P supply under N deficiency, nitrate (NO3 −) and ammonium (NH4 +) supply. Important Findings Females had a greater biomass, root length density (RLD), specific root length (SRL) and shoot P concentration than males under normal P availability with two N supplies. NH4 + supply led to higher total root length, RLD and SRL but lower root tip number than NO3 − supply under normal P supply. Under P deficiency, males showed a smaller root system but greater photosynthetic P availability and higher leaf P remobilization, exhibiting a better capacity to adaptation to P-deficiency than females. Under P deficiency, NO3 − supply increased leaf photosynthesis and PUE but reduced RLD and SRL in females while males had higher leaf P redistribution and photosynthetic PUE than NH4 + supply. Females had a better potentiality to cope with P deficiency under NO3 − supply than NH4 + supply; the contrary was true for males. These results suggest that females may devote to increase in P uptake and shoot P allocation under normal P availability, especially under NO3 − supply, while males adopt more efficient resource use and P remobilization to maximum their tolerance to P-deficiency.

scholarly journals Action of ethylene on postharvest of summer squash ‘Menina Brasileira’

Revista CERES ◽  
2017 ◽  
Vol 64 (4) ◽  
pp. 360-367
Author(s):  
Fernanda Ferreira de Araújo ◽  
Lucas Cavalcante da Costa ◽  
Tania Pires da Silva ◽  
Mário Puiatti ◽  
Fernando Luiz Finger
Keyword(s):  
Weight Loss ◽  
Chlorophyll Content ◽  
Electrolyte Leakage ◽  
Soluble Sugars ◽  
Control Treatment ◽  
Ethylene Concentration ◽  
Malondialdehyde Content ◽  
Summer Squash ◽  
No Weight Loss ◽  
Exogenous Ethylene

ABSTRACT The purpose of this study was to evaluate the sensitivity and the physiological responses of summer squash ‘Menina Brasileira’ to ethylene. Immature fruits were harvested and placed in 20 L sealed buckets, in which ethylene was applied at concentrations of 0.1, 1.0, 10, 100, and 1000 μL L-1 for 24 h. Fruits were placed in buckets with no ethylene as a control treatment. Thereafter, the fruits were taken out of the buckets and maintained on bench, wherein on days 0, 2, 4, 6, and 8, they were evaluated regarding the accumulated fresh weight loss, soluble sugars, reducing and non-reducing sugars, starch, total chlorophyll, content of malondialdehyde, and electrolyte leakage. Fruits of summer squash ‘Menina Brasileira’ showed sensitivity to exogenous ethylene with no weight loss stimulation. Additionally, the fruits exhibited small changes in nutritional quality attributes and changes in the external fruit appearance, including decreased chlorophyll content as well as damage to cell membrane characterized by increase in malondialdehyde content and electrolyte leakage. These changes were stimulated by increasing exogenous ethylene concentration.

scholarly journals Estimating the importance of maize root hairs in low phosphorus conditions and under drought

2019 ◽  
Vol 124 (6) ◽  
pp. 961-968 ◽  
Author(s):  
Florian Klamer ◽  
Florian Vogel ◽  
Xuelian Li ◽  
Hinrich Bremer ◽  
Günter Neumann ◽  
...  
Keyword(s):  
Root Hairs ◽  
Specific Root Length ◽  
P Uptake ◽  
Alkaline Soil ◽  
Secondary Effects ◽  
Short Root ◽  
P Deficiency ◽  
P Content ◽  
Low Phosphorus ◽  
P Supply

Abstract Background and Aims Root hairs are single-cell extensions of the epidermis that face into the soil and increase the root–soil contact surface. Root hairs enlarge the rhizosphere radially and are very important for taking up water and sparingly soluble nutrients, such as the poorly soil-mobile phosphate. In order to quantify the importance of root hairs for maize, a mutant and the corresponding wild type were compared. Methods The rth2 maize mutant with very short root hairs was assayed for growth and phosphorus (P) acquisition in a slightly alkaline soil with low P and limited water supply in the absence of mycorrhization and with ample P supply. Key Results Root and shoot growth was additively impaired under P deficiency and drought. Internal P concentrations declined with reduced water and P supply, whereas micronutrients (iron, zinc) were little affected. The very short root hairs in rth2 did not affect internal P concentrations, but the P content of juvenile plants was halved under combined stress. The rth2 plants had more fine roots and increased specific root length, but P mobilization traits (root organic carbon and phosphatase exudation) differed little. Conclusions The results confirm the importance of root hairs for maize P uptake and content, but not for internal P concentrations. Furthermore, the performance of root hair mutants may be biased by secondary effects, such as altered root growth.

Growth and the distribution of phosphorus in wheat developed under various phosphorus and temperature regimes

1986 ◽  
Vol 37 (5) ◽  
pp. 459 ◽  
Author(s):  
GD Batten ◽  
IF Wardlaw ◽  
MJ Aston
Keyword(s):  
Grain Yield ◽  
Dry Matter ◽  
Flag Leaf ◽  
Standard Condition ◽  
Growth Period ◽  
Phosphorus Concentration ◽  
Floral Initiation ◽  
Stages Of Development ◽  
P Deficiency ◽  
P Supply

Experiments were designed to examine the effect of the level and duration of application of phosphorus (P) on yield in wheat and the effect of growth conditions prior to anthesis on the utilisation of P taken up during the early stages of development. In the first experiment, wheat (Triticum aestivum cv. Kite) was grown in sand and supplied with a complete nutrient solution containing either 1 mM phosphate or 0.25 mM phosphate. The supply of P was maintained until grain maturity, or stopped at different stages of development (floral initiation, flag leaf emergence, anthesis). The increase in total plant dry matter over this period ranged from 8.8 to 17.6 g/plant, with the 1.0 mM P supply and from 4.1 to 9.5 g/plant with the 0.25 mM P supply. Supply of P beyond anthesis resulted in more tiller dry matter and increased the P content of the grain, but did not increase grain yield at either level. With 1 mM P to maturity, up to 21% P of the grain P could be attributed to retranslocation of P within the plant after anthesis. With 0.25 mM P to floral initiation, 58% of the grain P could be attributed to such retranslocation. In a second experiment plants (cv. Kite) were grown initially at 18/13�C with 0.25 mM P until floral initiation and thereafter with a P-free solution until maturity. Between floral initiation and anthesis plants were placed in six dayhight temperatures, extending (in 3�C steps) from 15/10�C to 30/25OC, and then returned to the standard condition of 18/13�C. Higher pre-anthesis temperatures reduced the pre-anthesis growth period and the plant height, but increased the leaf phosphorus concentration and uptake of phosphorus per plant in both the pre- and post-anthesis periods. Net CO2 exchange indicated that leaf senescence in P-deficient plants was closely associated with the export of nitrogen as well as the export of P. Grain P increased from 0.15% to 0.3% when the preanthesis temperature was increased from 15/10 to 30/25�C, although grain yield per main culm did not vary greatly. These findings highlight the importance of environmental conditions in determining the level of P deficiency in wheat, and show that grain yield is not limited by the amount of P in the grain.

scholarly journals Contrasting Growth, Photosynthesis, Antioxidant Responses and Water Use Efficiency in Two Medicago sativa L. Genotypes under Different Phosphorus and Soil Water Conditions

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1534
Author(s):  
Jing-Wei Fan ◽  
Xiao-Wei Yang ◽  
Tao Wang ◽  
Yuan Li ◽  
Hong Zhao ◽  
...  
Keyword(s):  
Plant Growth ◽  
Medicago Sativa ◽  
Soil Water ◽  
Early Screening ◽  
Soil Medium ◽  
P Deficiency ◽  
High Soil ◽  
Adaptive Mechanisms ◽  
Use Efficiency ◽  
Medicago Sativa L

Genotypic variations of alfalfa (Medicago sativa L.) to both phosphorus (P) deficiency and water deficiency are evident on the Loess Plateau of China. Here, we compare the adaptive mechanisms between an introduced cultivar (Arkaxiya) and a landrace (Longzhong) subjected to P- and water-limited conditions. The two genotypes were grown in a soil medium with 0, 4.2, 8.4 and 16.8 μg applied P per gram dry soil. Three water treatments were imposed (maintained at 75–90%, 45–55% and 30–35% of pot capacity (PC)) 28 days after sowing (DAS). At high soil P and high soil water content (SWC), high rates of net photosynthesis (Pn) contributed to greater plant growth and P-use efficiency (PUE) in the introduced Arkaxiya compared to the landrace Longzhong. However, at low SWC, Longzhong had enhanced antioxidative defense (mainly SOD and CAT) compared to Arkaxiya. In addition, shorter shoot length and greater branching in Longzhong than Arkaxiya may also facilitate adaptation to low SWC. The contrasting adaptive mechanisms of the two genotypes provide a number of early-screening parameters associated with plant growth for the selection and introduction of alfalfa targeted at different rainfall and available P environments.

Plant phosphorus status has a limited influence on the concentration of phosphorus-mobilising carboxylates in the rhizosphere of chickpea

2005 ◽  
Vol 32 (2) ◽  
pp. 153 ◽  
Author(s):  
Madeleine Wouterlood ◽  
Hans Lambers ◽  
Erik J. Veneklaas
Keyword(s):  
Root Systems ◽  
External Factors ◽  
Sand Culture ◽  
P Deficiency ◽  
Cicer Arietinum L ◽  
Split Root ◽  
Chickpea Cultivar ◽  
Phosphorus Status ◽  
Carboxylate Exudation ◽  
P Supply

Two experiments were conducted to investigate whether carboxylate exudation by chickpea (Cicer arietinum L.) is a response to phosphorus (P) deficiency or a constitutive trait. The effect of P supply on carboxylate concentrations in the plant and in the rhizosphere of chickpea cultivar Heera was studied in a sand culture. Plants were grown in pots supplied with 200 mL of solution containing 0–500 μm P every 3 d. Malonate was the main carboxylate exuded, and the main carboxylate in roots; shoots contained mainly citrate and malate. Contrary to what has been reported for other species, carboxylate concentrations in the rhizosphere decreased only slightly at high P supply, but they were still substantial. The effect of P supply on the rate of exudation was studied in a split-root sand culture. Root systems were split into two pots, one root half received no P and the other half received 200 mL of solution containing 0–500 μm P. The rhizosphere of both root halves contained similar concentrations of carboxylates, even when the plants received a different supply of P. Our results indicate that carboxylate exudation is determined by internal P rather than external factors. The fact that chickpea roots always exude carboxylates indicates that exudation in this species is largely constitutive.

Assessment of the phosphorus status of oilseed rape by plant analysis

1989 ◽  
Vol 29 (6) ◽  
pp. 861 ◽  
Author(s):  
A Pinkerton ◽  
K Spencer ◽  
AG Govaars
Keyword(s):  
Field Experiments ◽  
Maximum Yield ◽  
Plant Part ◽  
Plant Analysis ◽  
Triple Superphosphate ◽  
P Deficiency ◽  
P Concentration ◽  
Critical P Concentration ◽  
Phosphorus Status ◽  
P Supply

Phosphorus (P) concentrations in young plants of rapeseed (Brassica napus cv. Wesway) were related to seed and oil yields to develop a tissue test for the diagnosis of P deficiency. Critical P concentrations were defined as those concentrations required to sustain 90% of maximum yield. In 2 field experiments in successive seasons on a P-deficient soil, rates of triple superphosphate from 2.5 to 120 kg/ha were banded with the seed. The lowest P concentration in young shoots (17-19 weeks from sowing) associated with a P supply that was adequate for plant growth was approximately 0.31%. The youngest fully-expanded leaf was a reliable plant part to sample, its P concentration being about 0.05% lower than the concentration of the whole shoot. Critical P concentrations in young plants for sustaining 90% of maximum seed and oil yields were higher, namely 0.33 and 0.28% for whole shoots and youngest fully-expanded leaves respectively. The critical P concentration in seeds was about 0.35%.

scholarly journals Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency

2020 ◽  
Author(s):  
Jiajia Luo ◽  
Yunxi Liu ◽  
Huikai Zhang ◽  
Jinpeng Wang ◽  
Zhijian Chen ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Phosphorus Deficiency ◽  
Acid Soils ◽  
Pcr Analysis ◽  
P Deficiency ◽  
Adaptive Mechanisms ◽  
Pi Starvation ◽  
P Utilization ◽  
Metabolic Profiling Analysis ◽  
Underlying Mechanisms

Abstract Background: Phosphorus (P) deficiency is one of the major constraints limiting plant growth, especially in acid soils. Stylosanthes (stylo) is a pioneer tropical legume with excellent adaptability to low P stress, but its underlying mechanisms remain largely unknown.Results: In this study, the physiological, molecular and metabolic changes in stylo responding to phosphate (Pi) starvation were investigated. Under low P condition, the growth of stylo root was enhanced, which was attributed to the up-regulation of expansin genes participating in root growth. Metabolic profiling analysis showed that a total of 256 metabolites with differential accumulations were identified in stylo roots response to P deficiency, which mainly included flavonoids, sugars, nucleotides, amino acids, phenylpropanoids and phenylamides. P deficiency led to significant reduction in the accumulation of phosphorylated metabolites (e.g., P-containing sugars, nucleotides and cholines), suggesting that internal P utilization was enhanced in stylo roots subjected to low P stress. However, flavonoid metabolites, such as kaempferol, daidzein and their glycoside derivatives, were increased in P-deficient stylo roots. Furthermore, the qRT-PCR analysis showed that a set of genes involved in flavonoids synthesis were found to be up-regulated by Pi starvation in stylo roots. In addition, the abundances of phenolic acids and phenylamides were significantly increased in stylo roots during P deficiency. The increased accumulation of the metabolites in stylo roots, such as flavonoids, phenolic acids and phenylamides, might facilitate P solubilization and cooperate with beneficial microorganisms in rhizosphere, and thus contributing to P acquisition and utilization in stylo.Conclusions: These results suggest that stylo plants cope with P deficiency by modulating root morphology, scavenging internal Pi from phosphorylated metabolites and increasing accumulation of flavonoids, phenolic acids and phenylamides. This study provides valuable insights into the complex responses and adaptive mechanisms of stylo roots to P deficiency.

scholarly journals Species-Specific Responses of Root Morphology of Three Co-existing Tree Species to Nutrient Patches Reflect Their Root Foraging Strategies

2021 ◽  
Vol 11 ◽  
Author(s):  
Zhenya Yang ◽  
Benzhi Zhou ◽  
Xiaogai Ge ◽  
Yonghui Cao ◽  
Ivano Brunner ◽  
...  
Keyword(s):  
Tree Species ◽  
Root Length ◽  
Chinese Fir ◽  
Moso Bamboo ◽  
Foraging Strategies ◽  
Root Proliferation ◽  
Masson Pine ◽  
Root Foraging ◽  
Heterogeneous Soils ◽  
P Supply

Root foraging strategies of plants may be critical to the competition for nutrient resources in the nutrient patches, but little is known about these of co-existing tree species in subtropical regions. This study aimed to elucidate root foraging strategies of three co-existing tree species in nutrient heterogeneous soils by exploring their root distribution, root morphology, photosynthates allocation and nutrient accumulation. Seedlings of the three tree species [moso bamboo (Phyllostachys edulis), Chinese fir (Cunninghamia lanceolata), and masson pine (Pinus massoniana)] were grown for 8months under one homogeneous soil [uniform nitrogen (N) plus phosphorus (P)] and three heterogeneous soils (localized N supply, localized P supply, or localized N plus P supply). The biomass, root morphological parameters (i.e., root length and root surface area), specific root length (SRL), non-structural carbohydrates (NSCs, i.e., mobile sugar and starch) in roots, total N and total P of plants were measured. The plasticity and distribution of root system were analyzed by calculating the root response ratio (RRR) and root foraging precision (FP), respectively. The results are as follows (i) Chinese fir tended to forage more N by promoting root proliferation in the N-rich patch, while root proliferation of bamboo and pine did not change. For P, bamboo absorbed more P by promoting root proliferation in the P-rich patch. The total P content of Pine and Chinese fir under localized P supply treatment remain the same despite the fact that the root length in the P-rich patch and the FP increased. (ii) Chinese fir foraged more N by increasing root length and decreasing SRL in the NP-rich patch; bamboo foraged more N and P by increasing root length and SRL in the NP-rich patch. The FP and foraging scale (FS) of both bamboo and Chinese fir were significantly improved under localized N plus P treatment. (iii) The concentrations of NSC were positively correlated with root morphological plasticity for moso bamboo and Chinese fir. Our results indicated that higher morphological plasticity is exhibited in moso bamboo and Chinese fir than masson pine in nutrient heterogeneous soils, allowing them to successfully forage for more nutrients.

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