BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway

The plant resistance elicitor Benzo (1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) can enhance disease resistance of harvested fruit. Nonetheless, it is still unknown whether BTH plays a role in regulating fruit senescence. In this study, exogenous BTH treatment efficiently delayed the senescence of postharvest pitaya fruit with lower lipid peroxidation level. Furthermore, BTH-treated fruit exhibited lower hydrogen peroxide (H2O2) content, higher contents of reduced ascorbic acid (AsA) and reduced glutathione (GSH) levels and higher ratios of reduced to oxidized glutathione (GSH/GSSG) and ascorbic acid (AsA/DHA), as well as higher activities of ROS scavenging enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) and glutathione reductase (GR) in comparison with control fruit. Moreover, BTH treatment enhanced the activities of phenylpropanoid pathway-related enzymes, including cinnamate-4-hydroxylase (C4H), phenylalanine ammonia-lyase (PAL) and 4-coumarate/coenzyme A ligase (4CL) and the levels of phenolics, flavonoids and lignin. In addition, BTH treatment upregulated the expression of HuSOD1/3/4, HuCAT2, HuAPX1/2 and HuPOD1/2/4 genes. These results suggested that application of BTH delayed the senescence of harvested pitaya fruit in relation to enhanced antioxidant system and phenylpropanoid pathway.

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Roles of ROS scavenging enzymes in desiccation tolerance in ferns

South African Journal of Botany ◽

10.1016/j.sajb.2018.02.084 ◽

2018 ◽

Vol 115 ◽

pp. 299

Author(s):

K.G.W. Mkhize ◽

R.P. Beckett

Keyword(s):

Desiccation Tolerance ◽

Ros Scavenging ◽

Scavenging Enzymes ◽

Ros Scavenging Enzymes

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Elevated ROS-scavenging enzymes contribute to acclimation to UV-B exposure in transplastomic tobacco plants, reducing the role of plastid peroxidases

Journal of Plant Physiology ◽

10.1016/j.jplph.2016.05.026 ◽

2016 ◽

Vol 201 ◽

pp. 95-100 ◽

Cited By ~ 13

Author(s):

Gyula Czégény ◽

Bénédicte Le Martret ◽

Dóra Pávkovics ◽

Philip J. Dix ◽

Éva Hideg

Keyword(s):

Ros Scavenging ◽

Tobacco Plants ◽

Transplastomic Tobacco ◽

Scavenging Enzymes ◽

Ros Scavenging Enzymes

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Molecular mechanisms accompanying nitric oxide signalling through tyrosine nitration and S-nitrosylation of proteins in plants

Functional Plant Biology ◽

10.1071/fp16279 ◽

2018 ◽

Vol 45 (2) ◽

pp. 70 ◽

Cited By ~ 10

Author(s):

Prachi Jain ◽

Satish C. Bhatla

Keyword(s):

Nitric Oxide ◽

Molecular Mechanisms ◽

Stress Conditions ◽

Primary Metabolism ◽

Tyrosine Nitration ◽

Ros Scavenging ◽

Major Mechanism ◽

Tissue System ◽

Scavenging Enzymes ◽

Ros Scavenging Enzymes

Nitric oxide (NO) signalling in plants is responsible for modulation of a variety of plant developmental processes. Depending on the tissue system, the signalling of NO-modulated biochemical responses majorly involves the processes of tyrosine nitration or S-nitrosylation of specific proteins/enzymes. It has further been observed that there is a significant impact of various biotic/abiotic stress conditions on the extent of tyrosine nitration and S-nitrosylation of various metabolic enzymes, which may act as a positive or negative modulator of the specific routes associated with adaptive mechanisms employed by plants under the said stress conditions. In addition to recent findings on the modulation of enzymes of primary metabolism by NO through these two biochemical mechanisms, a major mechanism for regulating the levels of reactive oxygen species (ROS) under stress conditions has also been found to be through tyrosine nitration or S-nitrosylation of ROS-scavenging enzymes. Recent investigations have further highlighted the differential manner in which the ROS-scavenging enzymes may be S-nitrosylated and tyrosine nitrated, with reference to their tissue distribution. Keeping in mind the very recent findings on these aspects, the present review has been prepared to provide an analytical view on the significance of protein tyrosine nitration and S-nitrosylation in plant development.

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The Bacterial Superoxide Dismutase and Glutathione Reductase Are Crucial for Endophytic Colonization of Rice Roots by Gluconacetobacter diazotrophicus PAL5

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-12-12-0286-r ◽

2013 ◽

Vol 26 (8) ◽

pp. 937-945 ◽

Cited By ~ 76

Author(s):

Sylvia Alquéres ◽

Carlos Meneses ◽

Luc Rouws ◽

Michael Rothballer ◽

Ivo Baldani ◽

...

Keyword(s):

Superoxide Dismutase ◽

Glutathione Reductase ◽

Gluconacetobacter Diazotrophicus ◽

Ros Scavenging ◽

Endophytic Colonization ◽

Rice Roots ◽

Early Stages ◽

Cell Counts ◽

Scavenging Enzymes ◽

Ros Scavenging Enzymes

Gluconacetobacter diazotrophicus is an aerobic diazotrophic plant-growth-promoting bacterium isolated from different gramineous plants. We showed that reactive oxygen species (ROS) were produced at early stages of rice root colonization, a typical plant defense response against pathogens. The transcription of the pathogen-related-10 gene of the jasmonic acid (JA) pathway but not of the PR-1 gene of the salicylic acid pathway was activated by the endophytic colonization of rice roots by G. diazotrophicus strain PAL5. Quantitative polymerase chain reaction analyses showed that, at early stages of colonization, the bacteria upregulated the transcript levels of ROS-detoxifying genes such as superoxide dismutase (SOD) and glutathione reductase (GR). To proof the role of ROS-scavenging enzymes in the colonization and interaction process, transposon insertion mutants of the SOD and GR genes of strain PAL5 were constructed. The SOD and GR mutants were unable to efficiently colonize the roots, indicated by the decrease of tightly root-associated bacterial cell counts and endophytic colonization and by fluorescence in situ hybridization analysis. Interestingly, the mutants did not induce the PR-10 of the JA-pathway, probably due to the inability of endophytic colonization. Thus, ROS-scavenging enzymes of G. diazotrophicus strain PAL5 play an important role in the endophytic colonization of rice plants.

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Drought sensitive maize inbred shows more oxidative damage and higher ROS scavenging enzymes, but not glyoxalases than a tolerant one at seedling stage

Plant Omics ◽

10.21475/poj.16.09.04.pne31 ◽

2016 ◽

Vol 9 (4) ◽

pp. 220-232 ◽

Cited By ~ 6

Author(s):

Md. Motiar Rohman ◽

◽

Shahnewaz Begum ◽

M.Z.A. Talukder ◽

Afsana Hoque Akhi ◽

...

Keyword(s):

Oxidative Damage ◽

Seedling Stage ◽

Ros Scavenging ◽

Scavenging Enzymes ◽

Ros Scavenging Enzymes

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Correction: Zhang, Y., et al. Clones of FeSOD, MDHAR, DHAR Genes from White Clover and Gene Expression Analysis of ROS-Scavenging Enzymes during Abiotic Stress and Hormone Treatments. Molecules 2015, 20, 20939–20954

Molecules ◽

10.3390/molecules201219850 ◽

2015 ◽

Vol 20 (12) ◽

pp. 22236-22240

Author(s):

Yan Zhang ◽

Zhou Li ◽

Yan Peng ◽

Xiaojuan Wang ◽

Dandan Peng ◽

...

Keyword(s):

Gene Expression ◽

Abiotic Stress ◽

Expression Analysis ◽

White Clover ◽

Gene Expression Analysis ◽

Ros Scavenging ◽

Scavenging Enzymes ◽

Ros Scavenging Enzymes

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Overexpression ofCaAPXInduces Orchestrated Reactive Oxygen Scavenging and Enhances Cold and Heat Tolerances in Tobacco

BioMed Research International ◽

10.1155/2017/4049534 ◽

2017 ◽

Vol 2017 ◽

pp. 1-15 ◽

Cited By ~ 5

Author(s):

Jiangying Wang ◽

Bin Wu ◽

Hengfu Yin ◽

Zhengqi Fan ◽

Xinlei Li ◽

...

Keyword(s):

Stress Tolerance ◽

Cellular Response ◽

Plant Stress ◽

Reactive Oxygen ◽

Pcr Analysis ◽

Plant Tolerance ◽

Ros Scavenging ◽

Expression Levels ◽

Scavenging Enzymes ◽

Ros Scavenging Enzymes

Ascorbate peroxidase (APX) acts indispensably in synthesizing L-ascorbate (AsA) which is pivotal to plant stress tolerance by detoxifying reactive oxygen species (ROS). Enhanced activity of APX has been shown to be a key step for genetic engineering of improving plant tolerance. However it needs a deeper understanding on the maintenance of cellular ROS homeostasis in response to stress. In this study, we identified and characterized anAPX(CaAPX) gene fromCamellia azalea. Quantitative real-time PCR (qRT-PCR) analysis showed thatCaAPXwas expressed in all tissues and peaked in immature green fruits; the expression levels were significantly upregulated upon cold and hot stresses. Transgenic plants displayed marked enhancements of tolerance under both cold and heat treatments, and plant growth was correlated withCaAPXexpression levels. Furthermore, we monitored the activities of several ROS-scavenging enzymes includingCu/Zn-SOD,CAT,DHAR, andMDHAR, and we showed that stress tolerance was synchronized with elevated activities of ROS-scavenging. Moreover, gene expression analysis of ROS-scavenging enzymes revealed a role ofCaAPXto orchestrate ROS signaling in response to temperature stresses. Overall, this study presents a comprehensive characterization of cellular response related toCaAPXexpression and provides insights to breed crops with high temperature tolerances.

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