scholarly journals Phenolic Profile by HPLC-PDA-MS of Greek Chamomile Populations and Commercial Varieties and Their Antioxidant Activity

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2345
Author(s):  
Nektaria Tsivelika ◽  
Maria Irakli ◽  
Athanasios Mavromatis ◽  
Paschalina Chatzopoulou ◽  
Anastasia Karioti
Keyword(s):  
Antioxidant Activity ◽  
Herbal Remedy ◽  
Antioxidant Properties ◽  
Flavonol Glycosides ◽  
Phenolic Profile ◽  
Flavone Glycosides ◽  
Phenolic Constituents ◽  
Wide Range ◽  
Quantitative Results ◽  
Excellent Source

The phenolic profile of Greek chamomile populations was investigated by HPLC-PDA-MS. For comparison, three commercial varieties (Banatska, Lutea and Goral) cultivated under the same conditions were included in the study. All samples exhibited similar qualitative patterns but differed in their quantitative characteristics. Overall, 29 constituents were detected, belonging to phenolic acids, flavonol glycosides, flavone glycosides (mainly apigenin derivatives) and acylated polyamines. Quantitative results showed that both Greek populations had a high content in apigenin derivatives (0.39 and 0.31 %w/w) and caffeoylquinic acids (0.96 and 0.81 %w/w), whereas they had the highest amount of flavonol glycosides among the tested samples. Greek populations were comparable to the Banatska variety, while they were superior to the Lutea and Goral varieties cultivated under the same conditions. Results demonstrate that Greek chamomile populations studied here, are an excellent source of a wide range of phenolics which contribute to the medicinal and antioxidant properties of this herbal remedy. Antioxidant tests showed that chamomile extracts from the studied materials, especially from the Greek populations possess antioxidant activity, corresponding to their polyphenol content. This is the first report on the phenolic constituents of Matricaria recutita growing in Greece and well-established chamomile varieties.

scholarly journals Isobornylchalcones as Scaffold for the Synthesis of Diarylpyrazolines with Antioxidant Activity

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3579
Author(s):  
Svetlana A. Popova ◽  
Evgenia V. Pavlova ◽  
Oksana G. Shevchenko ◽  
Irina Yu. Chukicheva ◽  
Aleksandr V. Kutchin
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Biological Properties ◽  
High Reactivity ◽  
Biologically Active ◽  
Brain Lipids ◽  
Wide Range ◽  
Privileged Structure ◽  
Vitro Model

The pyrazoline ring is defined as a “privileged structure” in medicinal chemistry. A variety of pharmacological properties of pyrazolines is associated with the nature and position of various substituents, which is especially evident in diarylpyrazolines. Compounds with a chalcone fragment show a wide range of biological properties as well as high reactivity which is primarily due to the presence of an α, β-unsaturated carbonyl system. At the same time, bicyclic monoterpenoids deserve special attention as a source of a key structural block or as one of the pharmacophore components of biologically active molecules. A series of new diarylpyrazoline derivatives based on isobornylchalcones with different substitutes (MeO, Hal, NO2, N(Me)2) was synthesized. Antioxidant properties of the obtained compounds were comparatively evaluated using in vitro model Fe2+/ascorbate-initiated lipid peroxidation in the substrate containing brain lipids of laboratory mice. It was demonstrated that the combination of the electron-donating group in the para-position of ring B and OH-group in the ring A in the structure of chalcone fragment provides significant antioxidant activity of synthesized diarylpyrazoline derivatives.

scholarly journals RELATIONSHIP BETWEEN ANTIOXIDANT PROPERTIES AND NUTRITIONAL COMPOSITION OF SOME GALACTOPOIETICS HERBS USED IN INDONESIA: A COMPARATIVE STUDY

2016 ◽  
Vol 8 (12) ◽  
pp. 236 ◽  
Author(s):  
Ade Chandra Iwansyah ◽  
Rizal M. Damanik ◽  
Lilik Kustiyah ◽  
Muhammad Hanafi
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Total Phenolics ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Nutritional Composition ◽  
Total Flavonoids ◽  
Wide Range ◽  
Phenolics Content

<p><strong>Objective: </strong>The objectives of this research were to evaluate and compare the antioxidant activity; phenolics and flavonoids contents; and chemical composition of some galactopoietic herbs used in Indonesia and to find a possible relationship between antioxidant activity; phenolics, flavonoids and chemical composition of these plants.</p><p><strong>Methods: </strong>A wide range of analytical parameters was studied, including yield; pH; water activity (a<sub>w</sub>); total color difference; phenolics and flavonoids content; moisture; ash; protein; fat; carbohydrate; fiber; calcium; zinc; magnesium; potassium; iron content; and the data were analyzed by partial least squares (PLS) regression analysis and principal component analysis (PCA) to evaluate the correlation between the parameters and classification of the plants. Folin–cioceltaeu assay, aluminum chloride assay and DPPH scavenging assay were used to evaluate of total phenolics content and free radical scavenging activity of the ethanolic extract of galactopoietic herbs.</p><p><strong>Results: </strong>All of the galactagogue herbs, <em>viz</em>. <em>Plectranthus amboinicus </em>(Lour.) (PA), <em>Sauropus androgynus</em> (SA), <em>Moringa oleifera</em> (MO), and <em>Carica papaya</em> leaves (CP) exhibited good results of TPC, TFC and antioxidant activity. MO contained the highest average of total phenolics content (53.89 mg GAE/g). SA with a ratio of total flavonoids to total phenolics (TF/TP) which is 1.59 contained the highest average of total flavonoids and antioxidant activity (IC<sub>50</sub>), 43.63 mg QE/g and 126.13 μg/ml, respectively, followed by MO&gt;CA&gt;PA. Based on PLS and PCA analysis, antioxidant activity correlated with fat, protein, phenolics content, flavonoids content and pH, but only the total flavonoids content correlated strongly with antioxidant activity.<strong></strong></p><p><strong>Conclusion: </strong>This provides useful data about the studied relationships and chemical patterns in some galactopoietic herbs used and provides a helpful tool for understanding much more about the interpretation of results from the experiments and the mechanism of biosynthesis in plants.</p>

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF INDIGOFERA TINCTORIA L.

2018 ◽  
Vol 11 (6) ◽  
pp. 136
Author(s):  
Chandrasekaran Swaminathan
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Root Extracts ◽  
Wide Range ◽  
Indigofera Tinctoria

Objective: The present study was designed to evaluate the phytochemical composition and antibacterial and antioxidant potential of methanolic leaf and root extracts of Indigofera tinctoria L.Methods: Phytochemical analysis was done using standard methods. The methanolic leaf and root extracts of the plant were tested against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Enterobacter aerogenes, and Salmonella paratyphi B by cup-plate agar diffusion method. The free radical scavenging activities of the methanol extracts of leaves and roots were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.Results: Phytochemical screening revealed the presence of carbohydrates, reducing sugars, alkaloids, saponins, phenolic compounds, and flavonoids in methanolic leaf and root extracts. Methanolic leaf extracts of the plant exhibited antibacterial activity against a wide range of bacteria, but the root extracts failed to inhibit the tested bacterial pathogens. The antioxidant activity determination revealed that at 100 μg/ml, methanolic root extracts had the highest antioxidant activity (89.10%) on DPPH free radicals followed by methanolic leaf extracts (46.74%).Conclusion: The results of the present study conclude that the studied plant possesses broad-spectrum antibacterial and antioxidant properties and may act as a potent antioxidant for biological systems susceptible to free radical-mediated reactions. 

scholarly journals Examining the Performance of Two Extraction Solvent Systems on Phenolic Constituents from U.S. Southeastern Blackberries

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4001
Author(s):  
Xiaoxi Liao ◽  
Phillip Greenspan ◽  
Ronald B. Pegg
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Phenolics ◽  
Antioxidant Properties ◽  
Solvent System ◽  
Antioxidant Power ◽  
Extraction Solvent ◽  
Phenolic Constituents ◽  
Ferric Reducing Antioxidant Power ◽  
Solvent Systems

Two common extraction solvent systems, namely acidified aqueous methanol and acidified aqueous acetone, were used to extract blackberry phenolics, and the antioxidant properties of the recovered extracts were compared. The crude extracts were fractionated into low- and high-molecular-weight phenolics by Sephadex LH-20 column chromatography. The hydrophilic-oxygen radical absorbance capacity (H-ORACFL), ferric reducing antioxidant power (FRAP), and the cellular antioxidant activity (CAA) assays were employed as indices to assess antioxidant capacity of the extracts and their respective fractions. The methanolic solvent system displayed a greater efficiency at extracting anthocyanin and flavonol constituents from the blackberries, while the acetonic solvent system was better at extracting flavan-3-ols and tannins. Anthocyanins were the dominant phenolic class found in the blackberries with 138.7 ± 9.8 mg C3G eq./100 g f.w. when using methanol as the extractant and 114.6 ± 3.4 mg C3G eq./100 g f.w. when using acetone. In terms of overall antioxidant capacity of blackberry phenolics, the acetonic solvent system was superior. Though present only as a small percentage of the total phenolics in each crude extract, the flavan-3-ols (42.37 ± 2.44 and 51.44 ± 3.15 mg/100 g f.w. in MLF and ALF, respectively) and ellagitannins (5.15 ± 0.78 and 9.31 ± 0.63 mg/100 g f.w. in MHF and AHF, respectively) appear to account for the differences in the observed antioxidant activity between the two solvent systems.

scholarly journals Chemical Composition and, Cellular Evaluation of the Antioxidant Activity ofDesmodium adscendensLeaves

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
François Nsemi Muanda ◽  
Jaouad Bouayed ◽  
Abdelouaheb Djilani ◽  
Chunyan Yao ◽  
Rachid Soulimani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Uv Spectrophotometry ◽  
Total Anthocyanin ◽  
Rp Hplc ◽  
Therapeutic Value ◽  
Wide Range ◽  
Cellular Tests

Desmodium adscendensplant is widely used as juice or tea in various parts of the world against a wide range of diseases. This study determines the quality and the quantity of polyphenols, flavonoids, anthocyanins, and tannins inD. adscendensleaves by UV-spectrophotometry and RP-HPLC methods. In addition, the antioxidant capacity of these phenolic compounds is evaluated by ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic)), DPPH (2,2-diphenyl-1 picrylhydrazyl), and Cellular tests.D. adscendensleaves are mainly composite of flavonoid compounds with 12.8 mg of catechin equivalent (CE)/g dw. The amounts of total polyphenol compounds are 11.1 mg of gallic acid equivalent (GAE)/g dw. The quantity of total anthocyanin and total tannin compounds is not considerable 0.0182 mg CgE/g dw and 0.39 mg CE/g dw, respectively. A direct correlation between phenolic compounds and antioxidant activity is observed (R2=0.96). The RP-HPLC analyses reveal that the main phenolic compound identified in the methanol-water extract is quercetrin dihydrat (2.11 mg/mL). According to the results, it is observed thatD. adscendensleaves possess a considerable scavenging antioxidant and antiradical capacity, therefore these antioxidant properties might increase the therapeutic value of this medicinal plant.

scholarly journals Antioxidant Activities of Vaccinium vitis-idaea L. Leaves within Cultivars and Their Phenolic Compounds

Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 844 ◽  
Author(s):  
Lina Raudone ◽  
Gabriele Vilkickyte ◽  
Lina Pitkauskaite ◽  
Raimondas Raudonis ◽  
Rimanta Vainoriene ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Positive Influence ◽  
Phenolic Profile ◽  
Structure Activity ◽  
The Subject ◽  
Potential Use

Lingonberry leaves are the subject of numerous studies because of antioxidant properties, positive influence on the health and potential use in the prevention and treatment of chronic diseases. In this work, the radical scavenging, reducing, chelating activities, and phenolic composition of ten lingonberry leaves cultivars, one subspecies, and one variety were investigated. Furthermore, the antioxidant activity of individual phenolic compounds, that can be found in lingonberry leaves, were analyzed, and structure-activity relationship was determined. Wide diversity for phenolic profile and antioxidant properties of lingonberry leaves has been observed in the present material. Cultivars ‘Kostromskaja rozovaja’, ‘Rubin’, and Vaccinium vitis-idaea var. leucocarpum surpassed all others tested cultivars and lower taxa by contents of phenolic compounds and antioxidant activity. Leaves of lingonberry cultivars and lower taxa are rich in arbutin, flavonol glycosides, proanthocyanidins, and the latter were considered to be the major contributor to antioxidant properties of lingonberry leaves.

scholarly journals COLLECTION, IDENTIFICATION, MOLECULAR CHARACTERIZATION, AND ANTIOXIDANT ACTIVITY OF NON-GILLED MUSHROOMS COLLECTED FROM NORTH WESTERN HIMALAYAS

2018 ◽  
Vol 11 (10) ◽  
pp. 254
Author(s):  
Shaveta Singh ◽  
Astha Tripathi
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Molecular Taxonomy ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Morphological Identification ◽  
Western Himalayas ◽  
Wide Range ◽  
North Western

Objective: The objective of present research deals with collection, taxonomic, molecular taxonomy characterization, and antioxidant activity of wild non-gilled mushrooms sample collected from North Western Himalayas.Methods: The wild non-gilled mushroom samples were characterized for micro, morphological properties, and molecular identification. 1,1 diphenylpicrylhydrazyl (DPPH), nitric oxide (NO2), hydrogen peroxide (H2O2), and free radical scavenging assay were used to evaluate the antioxidant properties of selected wild non-gilled mushrooms.Results: The obtained results revealed that on the basis of microscopic and morphological identification all the four non-gilled mushrooms samples were tentatively identified as 2/15 (Auricularia sp.1), 6/15 (Auricularia sp.2), 32/15 (Ganoderma sp.), and 132/15 (Trametes sp.). The colony, mycelial, and basidiospore morphology of fungal isolate were evidence of Basidiomycetes family. All these four non-gilled mushrooms were sequenced. Sequences were submitted to National Center for Biotechnology Information (NCBI), and their accession number was MF770159 (2/15), MF774107 (6/15), MF770158 (32/15), and MF770160 (132/15). The phylogenetic analysis of 18S ribosomal deoxyribonucleic acid (18S rDNA) sequence of four non-gilled mushrooms was compared with other species and identified as Auricularia polytricha, Ganoderma lucidum, Auricularia auricula-judae, and Trametes elegans. The four non-gilled mushrooms extract exhibited DPPH, NO2, and H2O2 free radical scavenging activities. G. lucidum methanolic extract has the highest effect on free radicals in comparison to hexane effect.Conclusion: All the isolated four non-gilled mushrooms showed good antioxidant potential, and it can be concluded that these mushrooms are not only consumed but also have a wide range of medicinal properties which must further explode for future use.

scholarly journals COMPARATIVE CHARACTERISTICS OF THE INFLUENCE OF TECHNOLOGY OF EXTRACTION ON ANTIOXI-DANT PROPERTIES FOR THE FARRY AND FLOWERS OF THE TENDER (CRATAEGUS)

2020 ◽  
pp. 157-166
Author(s):  
Aigul Raisovna Valeeva ◽  
Nadezhda Viktorovna Makarova ◽  
Dinara Fanisovna Valiulina
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Antioxidant Properties ◽  
Triterpene Saponins ◽  
Restoring Force ◽  
Optimal Method ◽  
Force Method ◽  
Flavone Glycosides ◽  
Fatty Oil ◽  
Hawthorn Fruit

The purpose of this work is to determine the most optimal method for extracting hawthorn fruits and flowers (Crataegus) with antioxidant properties. Flowers hawthorn rich in chemicals (choline, essential oil, acetylcholine, trimethylamine, flavone glycosides, caffeic, chlorogenic, ursolic and other various acids) and hawthorn fruit consist of fatty oil, triterpene saponins, choline, acetylcholine, tannins, sorbitol and organic acids. In order to determine the most optimal method for extracting a complex of substances with antioxidant properties from hawthorn fruit and flower extracts, the content of dry substances, phenols, flavonoids was studied, and antioxidant activity was determined using two methods: by trapping free radicals and by using the FRAP (restoring force) method. By all indicators (the content of dry substances, phenols, flavonoids, antioxidant activity), studied in the work, hawthorn flowers had high values. In this work, three extraction technologies were used: traditional – infusion (37 °С, 2 h), and innovative – using microwave (800 W, 1 min) and ultrasonic irradiation (0.5 W, 2 h). Of all the types of extraction considered, the most efficient and effective, according to the results of experiments, is extraction using ultrasonic radiation.

scholarly journals Impact of Plant Origin on Eurasian Propolis on Phenolic Profile and Classical Antioxidant Activity

Biomolecules ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 68
Author(s):  
Piotr Okińczyc ◽  
Jarosław Widelski ◽  
Jakub Szperlik ◽  
Magdalena Żuk ◽  
Tomasz Mroczek ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Array Detector ◽  
Phenolic Profile ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Marker Compounds ◽  
Electron Donation

Propolis is a bee product with known medical properties, including antioxidant activity. The scope of the study is profiling 19 different Eurasian propolis samples (mostly from Russia and Kazakhstan, Kyrgyzstan, Poland, Ukraine, and Slovakia). Profiles of propolises were investigated by ultra-high-performance liquid chromatography–diode array detector–mass spectrometry (UPLC-DAD-MS). Classical antioxidant properties, which are based on electron donation mechanism, were assessed by DPPH, ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) assays. Total phenolic and flavonoid contents were also evaluated by colorimetric tests. Most of the samples exhibited significant content of polyphenols (from 30.28 to 145.24 mg GAE/g of propolis) and flavonoids (from 10.45 to 82.71 mg GAE/g of propolis). Most of the propolis samples exhibited potent antiradical (DPPH test—from 8.83 to 64.47 mg GAE/g of propolis) and reducing activity (FRAP test—from 0.08 to 1.17 mmol Fe2+/g of propolis). Based on the occurrence of marker compounds, propolis samples were classified as poplar, aspen–birch, aspen–poplar, and aspen–birch–poplar type. Main markers present in propolis of poplar (e.g., chrysin, pinocembrin, galangin, and 3-O-acetyl-pinobanksin), birch (ermanin and acacetin) and aspen (2-acetyl-1,3-di-p-coumaroylglycerol) origin were used. DPPH, FRAP, and ORAC tests results were correlated with flavonoids, total polyphenols, or the polyphenols other than flavonoids content. In term of activity, poplar propolis type was variable, while aspen–birch–poplar type usually exhibited high DPPH and FRAP activity.

scholarly journals Fruit of raspberry Rubus Idaeus L. as a source of functional ingredients (review)

2019 ◽  
Vol 48 (1) ◽  
pp. 5-14 ◽  
Author(s):  
Екатерина Жбанова ◽  
Ekaterina Zhbanova
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Properties ◽  
Rubus Idaeus ◽  
Anthocyanin Content ◽  
Total Antioxidant Activity ◽  
Red Raspberry ◽  
Total Anthocyanin ◽  
Wide Range ◽  
Total Antioxidant

The article gives a comparative analysis of the data obtained by Russian and foreign scientists considering the content of vitamins and other valuable substances in raspberry fruit. That determines high antioxidant properties of raspberry. The goal of the study was to generalize information which shows specific nature of that plant in relation to the complex of vitamins and polyphenol compounds which it contains. The author shows that fruit of raspberry R. idaeus L. consists of 5–40 mg/100 g of vitamin C, folic acid - 26-44 mg/100 g. Consumption of only 100 g of raspberries satisfies daily requirement of vitamin C (60 mg) by 8.3 - 66.7%, in folates (200 mg) by 13–22%. Total anthocyanin content in red raspberry varies within wide range (20–100 mg/100 g), in black raspberry – form 200 to 600 mg/100 g. Raspberry accumulates from 38 to 270 mg/100 g of ellagic acid. In small amounts the cultivated red raspberry consists of the following substances: carotenoids (β-carotene – 9.3 mg/100 g, zeaxanthin – 11 mg/100 g, lutein – 320 mg /100 g), vitamin E – 0.15–0.44 mg/100 g tocopherol equivalents, thiamine – 0,020 mg/100 g, riboflavin – 0.034 mg/100 g, niacin – 0.036 mg/100 g, pyridoxin – 0.05 mg/100 g, biotin – 5.7 mg/100 g. Total content of antioxidants in the cultivated raspberry fruit is 1.71 mg/g (standard quercetin). As a result of the analysis of different literary sources the author determined that raspberry antioxidant properties are mainly connected with high polyphenol content. Contribution of vitamin C into total antioxidant activity is relatively low. Though raspberry fruit chemical composition has already been studied quite well it is necessary to perform further more detailed research of different raspberry cultivars considering their total antioxidant activity as well as certain biochemical components which comprise antioxidant complex of raspberry fruit. The obtained results will form the base for further research aimed at investigating phytochemical compounds of fruit crops which have become an essential part of healthy human diet and developing nutraceutical products.

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