scholarly journals Pretreatment with NaCl Promotes the Seed Germination of White Clover by Affecting Endogenous Phytohormones, Metabolic Regulation, and Dehydrin-Encoded Genes Expression under Water Stress

2018 ◽  
Vol 19 (11) ◽  
pp. 3570
Author(s):  
Yiqin Cao ◽  
Linlin Liang ◽  
Bizhen Cheng ◽  
Yue Dong ◽  
Jiaqi Wei ◽  
...  
Keyword(s):  
Water Stress ◽  
Seed Germination ◽  
White Clover ◽  
Metabolic Regulation ◽  
Soluble Sugars ◽  
Germination Percentage ◽  
Membrane Lipid ◽  
Antioxidant Metabolism ◽  
Stress Damage ◽  
Seeds Germination

This study was designed to examine the effects of NaCl pretreatment on the seed germination of white clover (Trifolium repens cv. Ladino) under water stress induced by 19% polyethylene glycol (PEG) 6000. Lower concentrations of NaCl (0.5, 1, and 2.5 mM) pretreatment significantly alleviated stress-induced decreases in germination percentage, germination vigor, germination index, and radicle length of seedlings after seven days of germination under water stress. The soaking with 1 mM of NaCl exhibited most the pronounced effects on improving seed germination and alleviating stress damage. NaCl-induced seeds germination and growth could be associated with the increases in endogenous gibberellic acid (GA) and indole-3-acetic acid (IAA) levels through activating amylases leading to improved amylolysis under water stress. Seedlings pretreated with NaCl had a significantly lower osmotic potential than untreated seedlings during seed germination, which could be related to significantly higher soluble sugars and free proline content in NaCl-treated seedlings under water stress. For antioxidant metabolism, NaCl pretreatment mainly improved superoxide dismutase, peroxidase, ascorbate peroxidase, and glutathione reductase activities, transcript levels of FeSOD, APX, and DHAR, and the content of ascorbic acid, reduced glutathione, and oxidized glutathione during seed germination under water stress. The results indicated that seeds soaking with NaCl could remarkably enhance antioxidant metabolism, thereby decreasing the accumulation of reactive oxygen species and membrane lipid peroxidation during germination under water stress. In addition, NaCl-upregulated dehydrin-encoded genes SK2 expression could be another important mechanism of drought tolerance during seeds germination of white clover in response to water stress.

scholarly journals γ-Aminobutyric Acid (GABA) Priming Improves Seed Germination and Seedling Stress Tolerance Associated With Enhanced Antioxidant Metabolism, DREB Expression, and Dehydrin Accumulation in White Clover Under Water Stress

2021 ◽  
Vol 12 ◽  
Author(s):  
Min Zhou ◽  
Muhammad Jawad Hassan ◽  
Yan Peng ◽  
Lin Liu ◽  
Wei Liu ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
White Clover ◽  
Soluble Sugars ◽  
Aminobutyric Acid ◽  
Enzymatic Antioxidants ◽  
Antioxidant Metabolism ◽  
Limited Condition ◽  
The Impact ◽  
Seeds Germination

As an important plant growth regulator, the role of γ-aminobutyric acid (GABA) in regulating seeds germination was less well elucidated under water stress. The present study was conducted to investigate the impact of GABA pretreatment on seeds germination of white clover (Trifolium repens) under water deficient condition. Results demonstrated that seeds pretreated with 2μmol/l GABA significantly alleviated decreases in endogenous GABA content, germination percentage, germination potential, germination index, root length, and fresh weight along with marked reduction in mean germination time after 7days of germination under drought stress. In addition, seeds priming with GABA significantly increased the accumulation of soluble sugars, non-enzymatic antioxidants [reduced ascorbate, dehydroascorbic acid, oxidized glutathione (GSSG), and reduced glutathione (GSH)], and enzymes [superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathioe reductase, and monodehydroasorbate reductase (MDHR)] activities involved in antioxidant metabolism, which could be associated with significant reduction in osmotic potential and the accumulation of superoxide anion, hydrogen peroxide, electrical leakage, and malondialdehyde in seeds under drought stress. The GABA-pretreated seeds exhibited significantly higher abundance of dehydrin (DHN, 56 KDa) and expression levels of DHNs encoding genes (SK2, Y2K, Y2SK, and Dehydrin b) and transcription factors (DREB2, DREB3, DREB4, and DREB5) than the untreated seeds during germination under water-limited condition. These results indicated that the GABA regulated improvement in seeds germination associated with enhancement in osmotic adjustment, antioxidant metabolism, and DREB-related DHNs expression. Current study will provide a better insight about the GABA-regulated defense mechanism during seeds germination under water-limited condition.

scholarly journals Diethyl Aminoethyl Hexanoate Priming Ameliorates Seed Germination via Involvement in Hormonal Changes, Osmotic Adjustment, and Dehydrins Accumulation in White Clover Under Drought Stress

2021 ◽  
Vol 12 ◽  
Author(s):  
Muhammad Jawad Hassan ◽  
Wan Geng ◽  
Weihang Zeng ◽  
Muhammad Ali Raza ◽  
Imran Khan ◽  
...  
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
White Clover ◽  
Osmotic Adjustment ◽  
Soluble Sugars ◽  
Seed Vigor ◽  
Starch Degradation ◽  
Diethyl Aminoethyl Hexanoate ◽  
The Impact ◽  
Seeds Germination

Drought is a serious outcome of climate change reducing the productivity of forage species under arid and semi-arid conditions worldwide. Diethyl aminoethyl hexanoate (DA-6), a novel plant growth regulator, has proven to be involved in the amelioration of critical physiological functions in many agricultural crops under various abiotic stresses, but the role of the DA-6 in improving seed germination has never been investigated under drought stress. The present study was carried out to elucidate the impact of the DA-6 priming on seeds germination of white clover under drought stress. Results showed that seed priming with the DA-6 significantly mitigated the drought-induced reduction in germination percentage, germination vigor, germination index, seed vigor index, root length, shoot length, and fresh weight after 7 days of seed germination. The DA-6 significantly increased the endogenous indole-3-acetic acid, gibberellin, and cytokinin content with marked reduction in abscisic acid content in seedlings under drought stress. In addition, the DA-6 significantly accelerated starch catabolism by enhancing the activities of hydrolases contributing toward enhanced soluble sugars, proline content and ameliorated the antioxidant defense system to enhance the ability of reactive oxygen species scavenging under drought stress. Furthermore, exogenous DA-6 application significantly increased dehydrins accumulation and upregulated transcript levels of genes encoding dehydrins (SK2, Y2SK, or DHNb) during seeds germination under water deficient condition. These findings suggested that the DA-6 mediated seeds germination and drought tolerance associated with changes in endogenous phytohormones resulting in increased starch degradation, osmotic adjustment, antioxidants activity, and dehydrins accumulation during seed germination under water deficient condition.

scholarly journals Mobilization of reserves and germination of seeds of Erythrina velutina Willd. (Leguminosae - Papilionoideae) under different osmotic potentials

2012 ◽  
Vol 34 (4) ◽  
pp. 580-588 ◽  
Author(s):  
Renata Conduru Ribeiro Reis ◽  
Bárbara França Dantas ◽  
Claudinéia Regina Pelacani
Keyword(s):  
Water Stress ◽  
Seed Germination ◽  
Osmotic Potential ◽  
Soluble Sugars ◽  
Germination Percentage ◽  
Uniformity Coefficient ◽  
Carbon And Nitrogen ◽  
Germination Process ◽  
Mean Time ◽  
Osmotic Potentials

Some environmental factors, including water availability, may influence seed germination. This study investigated the germination of E. velutina seeds submitted to different osmotic potentials and mobilization of reserves during water-stress. Scarified seeds were arranged in paper rolls and soaked in solutions of Polyethylene Glycol (PEG) prepared in osmotic potentials 0.0, -0.2, -0.4, -0.6, and -0.8 MPa and kept into a seed germinator, at 25 °C, and 12/12 h photoperiod (L/D), during 10 days. The percentage, mean time, mean speed, germination speed index; as well as the germination uniformity coefficient were assessed. During germination process the total soluble sugars, reducing sugars, soluble protein, and total amino acids were quantified in the cotyledon, hypocotyl and radicle of soaked seeds and cotyledons of quiescent seeds (control). There was influence of osmotic potential on E. velutina seed germination. The germination percentage remained at high levels until -0.6 MPa and above this osmotic potential there has been no germination. The mobilization of stored reserves of carbon and nitrogen in E. velutina seeds was also influenced by water-stress. There was sensitiveness between -0.2 and -0.6 MPa; however, the degradation and the mobilization of reserves was slower when the osmotic potential decreased.

scholarly journals Physical characterization of Rhipsalis (Cactaceae) fruits and seeds germination in different temperatures and light regimes

2016 ◽  
Vol 76 (2) ◽  
pp. 367-373 ◽  
Author(s):  
A. B. Lone ◽  
R. C. Colombo ◽  
B. L. G. Andrade ◽  
L. S. A. Takahashi ◽  
R. T. Faria
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Light Regimes ◽  
Total Absence ◽  
Germination Characteristics ◽  
Different Temperatures ◽  
Medium Time ◽  
Seeds Germination

Abstract The germination characteristics of the native cactus species are poorly known, being the temperature and the light the factors that the most interferes in that process. Thus, the objective of the present work was to characterize the fruits and evaluate the influence of the temperature and the light in the seed germination of Rhipsalis floccosa, Rhipsalis pilocarpa and Rhipsalis teres. The tested constant temperatures were 15, 20, 25, 30 and 35 °C and the alternate of 20-30 °C and 25-35 °C in a photoperiod of 10 hours, and with determination of the most appropriate temperature, the germination was tested in light absence. The germination percentage, the index of germination speed and medium time of germination were evaluated. For R. floccosa, the highest germination percentage was at 20 °C. For R. pilocarpa and R. teres, the highest germination percentages occurred in 15 °C and 20 °C. There was correlation to germination percentage between the three species, indicating that they had similar germination behavior. Total absence of germination was verified for the three species in condition of light absence. In conclusion, the temperature of 20 °C is the most suitable for the seed germination of R. floccosa. For the species R. pilocarpa and R. teres, the temperatures of 15 and 20 °C are the most suitable.

GERMINATION OF UNTREATED AND PRIMED SEEDS IN RAPESEED (BRASSICA NAPUS VAR OLEIFERA DEL.) UNDER SALINITY AND LOW MATRIC POTENTIAL

2012 ◽  
Vol 48 (2) ◽  
pp. 238-251 ◽  
Author(s):  
ROBERTA PACE ◽  
PAOLO BENINCASA ◽  
MICHEL EDMOND GHANEM ◽  
MURIEL QUINET ◽  
STANLEY LUTTS
Keyword(s):  
Water Stress ◽  
Brassica Napus ◽  
Cell Elongation ◽  
Soluble Sugars ◽  
Germination Percentage ◽  
Lower Amount ◽  
Negative Effects ◽  
Matric Potential ◽  
Response To Stress ◽  
Matric Stress

SUMMARYSalt (NaCl) and matric stress (polyethylene glycol; PEG 6000) impacts on germination were analysed in two rapeseed (Brassica napus var oleifera Del.) cultivars (Exagone: salt and water stress tolerant; Toccata: salt and water stress sensitive), which had the same imbibition kinetics when incubated in distilled water. Final germination percentage (G) and time to reach 50% G (T50) were affected either by 325 mM NaCl or −0.9 MPa PEG in Toccata but not in Exagone. Cultivars did not differ for protein concentration and protease activity in any germination condition; thus, protein metabolism seems not to be implicated in response to stress. Hydroxurea and cytochalasine-D inhibited germination in Toccata exposed to −0.9 MPa PEG but not in Exagone, suggesting that Exagone tolerance could be due to a lower amount of damaged DNA and lower requirement for DNA replication due to cell elongation rather than cell division for radicle elongation. Soluble sugars concentration increased in salt-stressed seeds from Exagone more than in Toccata and this could have allowed osmotic adjustment and therefore cell elongation despite the presence of external NaCl. Priming treatments with gibberellic acid, kinetin, NaCl and PEG hastened seed germination under control conditions but had no effect or even negative effects on germination under both salt and matric stresses.

scholarly journals Osmopriming duration in Araçá-boi seeds germination

2016 ◽  
Vol 10 (1) ◽  
pp. 17
Author(s):  
Marcela Liege Da Silva ◽  
Edvan Alves Chagas ◽  
Elias Ariel de Moura ◽  
Pollyana Cardoso Chagas ◽  
Oscar José Smiderle ◽  
...  
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
Amazon Region ◽  
Factorial Analysis ◽  
Average Efficiency ◽  
Industrial Use ◽  
Seeds Germination

The Araca-boi is a native fruit of the Amazon region and its fruits have great potential for agro-industrial use due to the intrinsic characteristics of the pulp. The seeds are dormant and the primary cause could be due to the presence of inhibiting chemicals germination and can also be caused by the impermeability in tegument. The objective of this study was to assess the osmopriming efficiency promoted by Sucrose (C12H22O11) and Potassium Nitrate (KNO3 ) on germination of seeds of araca-boi in different immersion times. Thus, the seeds were osmoconditioned in following solutions: C12H22O11, KNO3 , KNO3 + C12H22O11 and H2 O, by period of 6, 12 and 24 h. Throughout of 70 days were evaluated the germination percentage and germination rate (index). The experiment is based on a completely randomized in a factorial analysis 4x3 with four replications of 10 seeds. The osmopriming of Araca-boi seed in KNO3 solution was shown to be efficient both to promote germination and the germination rate. The Araca-boi seeds osmoconditioned in KNO3 solution has an average efficiency of 99% in the germination percentage up to 70 days. The osmopriming period showed no influence on seed germination of the seeds of Araca-boi.

scholarly journals Assessment of Certain Plant Species degrading Total Petroleum Hydrocarbons in Contaminated Soil

2018 ◽  
Vol 1 (1) ◽  
pp. 69-82
Author(s):  
Shahrzad Khatibi ◽  
Hossein Mirseyed Hosseini
Keyword(s):  
Seed Germination ◽  
White Clover ◽  
Petroleum Hydrocarbons ◽  
Germination Rate ◽  
Cost Effective ◽  
Germination Percentage ◽  
Total Petroleum Hydrocarbons ◽  
Environment Friendly ◽  
Significant Difference ◽  
Uncontaminated Soil

Biological techniques, especially phytoremediation, have long been recognized as cost-effective and environment friendly to eliminate pollutants from soil. This article is based on a study conducted to assess the capability of alfalfa, ryegrass and white clover to remove total petroleum hydrocarbons (TPHs) from soil. The presence of petroleum contamination significantly decreased germination percentage and rate along with biomass of alfalfa and white clover compared to uncontaminated soil. With regards to ryegrass, there was no significant difference in seed germination percentage and biomass, although the presence of petroleum decreased seed germination rate. The results indicated that these plants had effect on TPHs remediation; and removal of TPH from soil was directly related to density levels and time. Therefore, alfalfa and ryegrass in their highest density levels reduced the maximum concentration of TPHs at the end of the experiment by almost 64.41% and 60.36%, respectively, whereas only slight changes were observed in non-vegetated soil.

scholarly journals Stratification Improves Seed Germination of Five Native Wildflower Species

HortScience ◽  
1993 ◽  
Vol 28 (9) ◽  
pp. 899-901 ◽  
Author(s):  
Carlma B. Bratcher ◽  
John M. Dole ◽  
Janet C. Cole
Keyword(s):  
Seed Germination ◽  
Seed Viability ◽  
Echinacea Purpurea ◽  
Germination Percentage ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Purple Coneflower ◽  
Viable Seeds ◽  
Seeds Germination

The germination responses of wild blue indigo [Baptisia australis (L.) R. Br.], purple coneflower [Echinacea purpurea (L.) Moench.], Maximilian sunflower (Helianthus maximiliani Schrad.), spike goldenrod (Solidago petiolaris Ait.), and Missouri ironweed (Vernonia missurica Raf.) seeds after 0, 2, 4, 6, 8, or 10 weeks of stratification at 5C were investigated. Seed viability was determined using triphenyl tetrazolium chloride staining and germination based on the percentage of viable seeds. Germination percentage (GP) increased in all five species as weeks of stratification increased. Days to first germination and germination range (days from first to last germinating seed) decreased with increasing weeks of stratification, but the effect beyond 4 to 6 weeks was minimal. The number of weeks of stratification for maximum GP was 4 for purple coneflower, 6 for Maximilian sunflower, 8 for Missouri ironweed, and 10 for wild blue indigo and spike goldenrod.

scholarly journals Effects of Storage and Exogenous Ga3 on Lychee Seed Germination

2000 ◽  
Vol 43 (4) ◽  
pp. 441-445 ◽  
Author(s):  
Elizabeth Orika Ono ◽  
Sarita Leonel ◽  
Jayme Duarte Filho ◽  
João Domingos Rodrigues
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Storage Time ◽  
Storage Period ◽  
Germination Percentage ◽  
Germination Capacity ◽  
Seeds Germination

The effects of storage time and exogenous gibberellic acid on lychee seeds germination were studied. The seeds were removed from ripe fruits, washed, dried, stored at 8°C for 0, 15 and 30 days, and soaked during 24 hours in water and GA3 at 50, 100 and 200 mg.L-1 solutions. As the storage period increased, the germination capacity was lost, and the seeds had a short germinability after 30-day storage period. Gibberellic acid had not significant effect on enhancing both the germination percentage and rate.

scholarly journals Soluble sugars and germination of Annona emarginata (Schltdl.) H. Rainer seeds submitted to immersion in GA3 up to different water contents

2014 ◽  
Vol 36 (spe1) ◽  
pp. 281-287 ◽  
Author(s):  
Juliana Iassia Gimenez ◽  
Gisela Ferreira ◽  
Jaqueline Malagutti Corsato
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Water Content ◽  
Plant Growth Regulator ◽  
Soluble Sugars ◽  
Germination Percentage ◽  
Initial Water Content ◽  
Germination Test ◽  
Initial Water ◽  
Water Contents

The objective of this study was to evaluate the effect of different water contents achieved by Annona emarginata (Schltdl.) H. Rainer seeds during immersion in GA3 solutions, in variation of soluble sugars levels and germination. Seeds with 10% of initial water content were submitted to imbibition in GA3 solutions with concentrations of 0; 250; 500; 750 and 1000 mg L-1 and when they reached the water content of 15%, 20%, 25%, 30% and 35%, the quantification of soluble sugars levels and germination test were performed. Seeds immersed up to they reach 15% of water with GA3 and immersed up to the water acquisition of 20% without GA3, presented higher soluble sugars levels and germination percentage, which were decreased when the seeds reached 30% and 35% of water, independently of the presence of the plant growth regulator. It was conclude that different water contents reached by the seeds in immersion treatments with GA3 affect the soluble sugars levels and germination percentage of Annona emarginata seeds. Thus, in treatments with Annona emarginata, the seeds must remain immersed in water without GA3 up to they reach 20% of water, as higher water contents (35%) reduce the soluble sugars levels and the seed germination percentage.

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