scholarly journals New Insight on Water Status in Germinating Brassica napus Seeds in Relation to Priming-Improved Germination

2019 ◽  
Vol 20 (3) ◽  
pp. 540 ◽  
Author(s):  
Katarzyna Lechowska ◽  
Szymon Kubala ◽  
Łukasz Wojtyla ◽  
Grzegorz Nowaczyk ◽  
Muriel Quinet ◽  
...  

Seed priming is a pre-sowing method successfully used to improve seed germination. Since water plays a crucial role in germination, the aim of this study was to investigate the relationship between better germination performances of osmoprimed Brassica napus seeds and seed water status during germination. To achieve this goal, a combination of different kinds of approaches was used, including nuclear magnetic resonance (NMR) spectroscopy, TEM, and SEM as well as semi-quantitative PCR (semi-qPCR). The results of this study showed that osmopriming enhanced the kinetics of water uptake and the total amount of absorbed water during both the early imbibition stage and in the later phases of seed germination. The spin–spin relaxation time (T2) measurement suggests that osmopriming causes faster water penetration into the seed and more efficient tissue hydration. Moreover, factors potentially affecting water relations in germinating primed seeds were also identified. It was shown that osmopriming (i) changes the microstructural features of the seed coat, e.g., leads to the formation of microcracks, (ii) alters the internal structure of the seed by the induction of additional void spaces in the seed, (iii) increases cotyledons cells vacuolization, and (iv) modifies the expression pattern of aquaporin genes.

Author(s):  
Shrestha A ◽  

Black gram despite being one of the major grain legumes in the mid-hills of Nepal, poor germination is a problem in its cultivation. Seed priming is an accepted technique to improve seed germination. An experiment was therefore, conducted to study effect of priming using various priming agents on germination characteristics of Black gram.


2006 ◽  
Vol 86 (1) ◽  
pp. 143-148 ◽  
Author(s):  
W. D. Zhang ◽  
J. J. Bi ◽  
T. Y. Ning ◽  
X. L. Liu ◽  
M. R. He

Leymus chinensis (Trin.) Tzvel., a perennial cool-season grass distributed widely in eastern Asia, is an ideal species for the rangeland industry and soil conservation, but the seed has a long dormant period and low germination rates. This study examined the effects of temperature, light and various treatments including stratification, H2SO4, NaOCl, gibberellic acid (GA3) and 6-benzyl adenine (BA), on the germination of Leymus chinensis seed. Seed stored over 1 yr and germinated at a temperature regime of 25/15°C with a 12/12-h photoperiod germinated best, and had a germination rate of 30.3%, with a minimum germination time of 12 d. In these germination conditions, germinated seeds were counted after 15 d to determine the effects of other treatments upon freshly harvested seed germination. The final germination rate for freshly harvested seeds stratified at 4°C for 0 to 10 wk ranged from 8.5 to 37.3%. Germination rates were 14.0 and 14.8% when seeds were treated with 70% H2SO4 for 3 and 6 min, respectively. Seed treated with 5.0% NaOCl germinated 17.5 and 16.0% when treated for 10 and 20 min, respectively. When seeds were soaked in 1000 mg L-1 GA3 solution for 24 h, 42.3% germinated, while treatment with 50 mg L-1 of BA resulted in 50.3% germination. The results indicate that various dormancy mechanisms may exist in the species. These findings will be helpful to farmers and breeders in selecting suitable treatments to improve seed germination and establishment. Key words: Seed dormancy, grassland and sand-dune reclamation, photoperiod, Northern China, Poaceae, seed priming


2021 ◽  
Vol 9 (3) ◽  
pp. 110-113

A pot experiment was conducted to investigate the effect of priming with the extract of plant Calotropis procera on seed germination of Sorghum bicolor. The seeds of Sorghum bicolor were primed with different concentrations of Calotropis procera leaf extract and milky sap exudate for five days at different time intervals. In total, there were five groups of days and nine seed priming treatments. The result showed that maximum seeds were germinated on day 4 in all treatments, but it was not significantly different from day 2, 3, and day 5. Whereas the highest germination of seeds was noted in two treatments, 24 hours priming with 100% boiled leaves extract (T3) and 24 hours priming with 100% milky exudate (T4), irrespective of priming days, except one day. These results showed that both milky exudate and boiled leaf extract have a positive effect on seed germination of Sorghum bicolor; therefore, in the future, both types of extracts of Calotropis procera should be considered important for priming the seeds of different plants to improve seed germination.


Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2482
Author(s):  
Jarosław Konior ◽  
Mariusz Rejment

The degree of technical wear of old buildings, which are made of basic materials (cement, concrete, steel, timber, plaster, brick) using traditional technology, is expressed by the size and intensity of damage to their elements. The topic of the research concerns old residential buildings from the turn of the 19th and 20th centuries, which are located in the downtown district of Wroclaw, Poland. The descriptive analysis and the analysis of the definitions of defects that occur in the elements of residential buildings, which were performed as random analyzes, do not allow defects to be considered as measurable variables at a level of visual investigation. The major drawback of the method that is used by experts when assessing the technical condition of civil engineering buildings is that it does not numerically express the magnitude (strength) of the defects. Therefore, an attempt was made to numerically express the relationship (if such a relationship exists) between the occurred defects of buildings and the extent of their technical wear process. When calculating the strength of this relationship, the method of determining the point biserial correlation coefficient for the measurable property and the dichotomous property was used. It was found that the direction of the relation is right-hand for all the tested building elements, but the strength of the correlation between the detected defects and technical wear shows a considerable span and depends on the conditions of the apartment house’s maintenance. As a rule, damage caused by water penetration and moisture penetration always shows correlations of at least moderate strength.


2021 ◽  
Vol 756 (1) ◽  
pp. 012047
Author(s):  
M P Anwar ◽  
R Jahan ◽  
M R Rahman ◽  
A K M M Islam ◽  
F M J Uddin

1952 ◽  
Vol 25 (1) ◽  
pp. 21-32 ◽  
Author(s):  
W. C. Warner ◽  
J. Reid Shelton

Abstract Three olefins were oxidized in the liquid phase with molecular oxygen to determine the kinetics of the oxidation reactions and the relationship to oxidation of rubber. The instantaneous rate of oxidation was found to be related to the analytically determined olefin and peroxide concentrations by the equation : Rate=k (unreacted olefin)(peroxide), where rate equals moles of oxygen per mole of original olefin per hour and the parentheses represent molarities. Presence of a phenyl group was found to affect k, but only in a minor way, indicating that the same fundamental kinetic mechanism applies in both aromatic and aliphatic olefins. The data are consistent with the general kinetic mechanism of Bolland involving oxygen attack at the alpha-methylenic group. However, it appears probable that initial oxygen attack can also occur at the double bond, resulting in the formation of a peroxide biradical, which may then react with other olefin molecules, initiating the usual chain reaction mechanism.


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