scholarly journals Genetic Identification and Transcriptome Analysis of Lintless and Fuzzless Traits in Gossypium arboreum L.

2020 ◽  
Vol 21 (5) ◽  
pp. 1675 ◽  
Author(s):  
Xueying Liu ◽  
Philippe Moncuquet ◽  
Qian-Hao Zhu ◽  
Warwick Stiller ◽  
Zhengsheng Zhang ◽  
...  
Keyword(s):  
Single Cells ◽  
Genetic Identification ◽  
Acceptor Site ◽  
Tetraploid Cotton ◽  
Gossypium Arboreum ◽  
Hub Genes ◽  
Potential Donor ◽  
Cotton Fibres ◽  
Cotton Species ◽  
Final Length

Cotton fibres, as single cells arising from the seed coat, can be classified as lint and fuzz according to their final length. Gossypium arboreum is a cultivated diploid cotton species and a potential donor of the A subgenome of the more widely grown tetraploid cottons. In this study, we performed genetic studies on one lintless and seven fuzzless G. arboreum accessions. Through association and genetic linkage analyses, a recessive locus on Chr06 containing GaHD-1 was found to be the likely gene underlying the lintless trait. GaHD-1 carried a mutation at a splicing acceptor site that resulted in alternative splicing and a deletion of 247 amino acid from the protein. The regions containing GaGIR1 and GaMYB25-like were found to be associated with fuzz development in G. arboreum, with the former being the major contributor. Comparative transcriptome analyses using 0-5 days post-anthesis (dpa) ovules from lintless, fuzzless, and normal fuzzy seed G. arboreum accessions revealed gene modules and hub genes potentially important for lint and fuzz initiation and development. Three significant modules and 26 hub genes associated with lint fibre initiation were detected by weighted gene co-expression network analysis. Similar analyses identified three vital modules and 10 hub genes to be associated with fuzz development. The findings in this study contribute to understanding the complex molecular mechanism(s) regulating fibre initiation and development and indicate that G. arboreum may have fibre developmental pathways different from tetraploid cotton. It also provides candidate genes for further investigation into modifying fibre development in G. arboreum.

scholarly journals Recent Developments in Fiber Genomics of Tetraploid Cotton Species

2018 ◽  
Author(s):  
Mirzakamol S. Ayubov ◽  
Ibrokhim Y. Abdurakhmonov ◽  
Venkateswara R. Sripathi ◽  
Sukumar Saha ◽  
Tokhir M. Norov ◽  
...  
Keyword(s):  
Tetraploid Cotton ◽  
Cotton Species ◽  
Recent Developments

scholarly journals Identification of a genome-specific repetitive element in the Gossypium D genome

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8344
Author(s):  
Hejun Lu ◽  
Xinglei Cui ◽  
Yanyan Zhao ◽  
Richard Odongo Magwanga ◽  
Pengcheng Li ◽  
...  
Keyword(s):  
Repetitive Element ◽  
Repetitive Sequences ◽  
Long Terminal Repeat Retrotransposons ◽  
Gossypium Arboreum ◽  
D Genome ◽  
Cotton Species ◽  
A Genome ◽  
Gossypium Raimondii ◽  
The Common

The activity of genome-specific repetitive sequences is the main cause of genome variation between Gossypium A and D genomes. Through comparative analysis of the two genomes, we retrieved a repetitive element termed ICRd motif, which appears frequently in the diploid Gossypium raimondii (D5) genome but rarely in the diploid Gossypium arboreum (A2) genome. We further explored the existence of the ICRd motif in chromosomes of G. raimondii, G. arboreum, and two tetraploid (AADD) cotton species, Gossypium hirsutum and Gossypium barbadense, by fluorescence in situ hybridization (FISH), and observed that the ICRd motif exists in the D5 and D-subgenomes but not in the A2 and A-subgenomes. The ICRd motif comprises two components, a variable tandem repeat (TR) region and a conservative sequence (CS). The two constituents each have hundreds of repeats that evenly distribute across 13 chromosomes of the D5genome. The ICRd motif (and its repeats) was revealed as the common conservative region harbored by ancient Long Terminal Repeat Retrotransposons. Identification and investigation of the ICRd motif promotes the study of A and D genome differences, facilitates research on Gossypium genome evolution, and provides assistance to subgenome identification and genome assembling.

scholarly journals Morphological description of a novel synthetic allotetraploid(A1A1G3G3) of Gossypium herbaceum L.and G.nelsonii Fryx. suitable for disease-resistant breeding applications

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0242620
Author(s):  
Xiaomin Yin ◽  
Rulin Zhan ◽  
Yingdui He ◽  
Shun Song ◽  
Lixia Wang ◽  
...  
Keyword(s):  
Chromosome Doubling ◽  
Diploid Species ◽  
Morphological Description ◽  
Tetraploid Cotton ◽  
Distant Hybridization ◽  
Cotton Species ◽  
Cotton Plants ◽  
Wild Cotton ◽  
Diploid Cotton ◽  
Gossypium Herbaceum

Wild species of Gossypium ssp. are an important source of traits for improving commercial cotton cultivars. Previous reports show that Gossypium herbaceum L. and Gossypium nelsonii Fryx. have better disease resistance characteristics than commercial cotton varieties. However, chromosome ploidy and biological isolation make it difficult to hybridize diploid species with the tetraploid Gossypium hirsutum L. We developed a new allotetraploid cotton genotype (A1A1G3G3) using a process of distant hybridization within wild cotton species to create new germplasms. First of all, G. herbaceum and G. nelsonii were used for interspecific hybridization to obtain F1 generation. Afterwards, apical meristems of the F1 diploid cotton plants were treated with colchicine to induce chromosome doubling. The new interspecific F1 hybrid and S1 cotton plants originated from chromosome duplication, were tested via morphological and molecular markers and confirmed their tetraploidy through flowrometric and cytological identification. The S1 tetraploid cotton plants was crossed with a TM-1 line and fertile hybrid offspring were obtained. These S2 offsprings were tested for resistance to Verticillium wilt and demonstrated adequate tolerance to this fungi. The results shows that the new S1 cotton line could be used as parental material for hybridization with G. hirsutum to produce pathogen-resistant cotton hybrids. This new S1 allotetraploid genotype will contributes to the enrichment of Gossypium germplasm resources and is expected to be valuable in polyploidy evolutionary studies.

scholarly journals Genome-wide identification and analysis of Oleosin gene family in four cotton species and its involvement in oil accumulation and germination

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yanchao Yuan ◽  
Xinzhe Cao ◽  
Haijun Zhang ◽  
Chunying Liu ◽  
Yuxi Zhang ◽  
...  
Keyword(s):  
Seed Germination ◽  
Subcellular Localization ◽  
Gene Family ◽  
Oil Content ◽  
Seed Oil ◽  
Tetraploid Cotton ◽  
Oil Bodies ◽  
Seed Oil Content ◽  
Cotton Species ◽  
And Function

Abstract Background Cotton is not only a major textile fiber crop but also a vital oilseed, industrial, and forage crop. Oleosins are the structural proteins of oil bodies, influencing their size and the oil content in seeds. In addition, the degradation of oleosins is involved in the mobilization of lipid and oil bodies during seed germination. However, comprehensive identification and the systematic analysis of the Oleosin gene (OLEOs) family have not been conducted in cotton. Results An in-depth analysis has enabled us to identify 25 and 24 OLEOs in tetraploid cotton species G. hirsutum and G. barbadense, respectively, while 12 and 13 OLEOs were identified in diploid species G. arboreum and G. raimondii, respectively. The 74 OLEOs were further clustered into three lineages according to the phylogenetic tree. Synteny analysis revealed that most of the OLEOs were conserved and that WGD or segmental duplications might drive their expansion. The transmembrane helices in GhOLEO proteins were predicted, and three transmembrane models were summarized, in which two were newly proposed. A total of 24 candidate miRNAs targeting GhOLEOs were predicted. Three highly expressed oil-related OLEOs, GH_A07G0501 (SL), GH_D10G0941 (SH), and GH_D01G1686 (U), were cloned, and their subcellular localization and function were analyzed. Their overexpression in Arabidopsis increased seed oil content and decreased seed germination rates. Conclusion We identified OLEO gene family in four cotton species and performed comparative analyses of their relationships, conserved structure, synteny, and gene duplication. The subcellular localization and function of three highly expressed oil-related OLEOs were detected. These results lay the foundation for further functional characterization of OLEOs and improving seed oil content.

scholarly journals Genetic Diversity of the Two Commercial Tetraploid Cotton Species in theGossypiumDiversity Reference Set

2016 ◽  
Vol 107 (3) ◽  
pp. 274-286 ◽  
Author(s):  
Lori L. Hinze ◽  
Elodie Gazave ◽  
Michael A. Gore ◽  
David D. Fang ◽  
Brian E. Scheffler ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Tetraploid Cotton ◽  
Reference Set ◽  
Cotton Species

Comparative evaluation of Gossypium arboreum L. and Gossypium hirsutum L. genotypes for drought tolerance

2019 ◽  
Vol 17 (6) ◽  
pp. 506-513
Author(s):  
Muhammad Iqbal ◽  
Mueen Alam Khan ◽  
Waqas Shafqat Chattha ◽  
Khalid Abdullah ◽  
Asif Majeed
Keyword(s):  
Drought Tolerance ◽  
Gossypium Hirsutum ◽  
Negative Impact ◽  
Primary Root ◽  
Dry Weight ◽  
Fibre Strength ◽  
Gossypium Arboreum ◽  
Cotton Production ◽  
Chlorophyll Contents ◽  
Cotton Species

AbstractDrought stress negatively affects the cotton production all over the world. The negative impact of drought varies for different species due to some morphological and root attributes that help some species to better stand under drought. But the extent of disturbance varies for different cotton species. To find out such variation, two cotton species (Gossypium hirsutum and Gossypium arboreum) were studied under normal and drought conditions for 2 years. Two genotypes for each species were included, i.e. PC-1 and COMILLA (G. arboreum) and IUB-13 and IUB-65 (G. hirsutum). The experiment was laid out under a completely randomized design following factorial arrangement. Genotype × treatment × year interaction of cotton genotypes was studied for different root, morphological, physiological and fibre-related traits. Traits such as above ground dry biomass, above ground fresh biomass, chlorophyll contents, leaf area, seed cotton yield, sympodial branches/plant, fibre strength and ginning out-turn were higher in G. hirsutum genotypes as compared to G. arboreum genotypes. However less reduction under drought in all above mentioned traits was recorded for G. arboreum, than G. hirsutum. Furthermore, root traits; primary root length, lateral root numbers, root fresh weight and root dry weight were enriched under drought condition in G. arboreum genotypes than in G. hirsutum genotypes, which is a clear manifestation of higher drought tolerance ability in G. arboreum genotypes transferrable to G. hirsutum genotypes through interspecific crossing or other means.

Analysis of genetic diversity, population structure and linkage disequilibrium in elite cotton (Gossypium L.) germplasm in India

2011 ◽  
Vol 62 (10) ◽  
pp. 859 ◽  
Author(s):  
Satya Narayan Jena ◽  
Anukool Srivastava ◽  
Uma Maheswar Singh ◽  
Sribash Roy ◽  
Nandita Banerjee ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Linkage Disequilibrium ◽  
Diploid Species ◽  
Aflp Markers ◽  
Tetraploid Cotton ◽  
Cotton Species ◽  
Geographical Regions ◽  
Wide Range ◽  
Diploid Cotton ◽  
Efficient Breeding

An understanding of the level of genetic diversity is a prerequisite for designing efficient breeding programs. Fifty-one cultivars of four cotton species (Gossypium hirsutum, G. barbadense, G. herbaceum and G. arboreum) representing core collections at four major cotton research stations with a wide range of eco-geographical regions in India were examined for the level of genetic diversity, distinct subpopulations and the level of linkage disequilibrium (LD) using 1100 amplified fragment length polymorphism (AFLP) markers with 16 primer pairs combinations. The AFLP markers enabled a reliable assessment of inter- and intra-specific genetic variability with a heterogeneous genetic structure. Higher genetic diversity was noticed in G. herbaceum, followed by G. arboreum. The genetic diversity in tetraploid cotton species was found to be less than that in the diploid species. The genotypes VAGAD, RAHS14, IPS187, 221 557, Jayhellar of G. herbaceum and 551, DLSA17, 221 566 of G. arboreum were identified as the most diverse parents, useful for quantitative trait loci (QTL) analysis in diploid cotton. Similarly, LRA 5166, AS3 and MCU5 of G. hirsutum and B1, B3, Suvin of G. barbadense were most diverse to develop mapping populations for fibre quality. The internal transcribed spacer sequences were sufficient to resolve different species and subspecies of diploid cotton. Low level of genome-wide LD was detected in the entire collection (r2 = 0.07) as well as within the four species (r2 = 0.11–0.15). A strong agreement was noticed between the clusters constructed on the basis of morphological and genotyping data.

scholarly journals Subgenome Bias and Temporal Postponement of Gene Expression Contributes to the Distinctions of Fiber Quality in Gossypium Species

2021 ◽  
Vol 12 ◽  
Author(s):  
Huan Mei ◽  
Bowen Qi ◽  
Zegang Han ◽  
Ting Zhao ◽  
Menglan Guo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fiber Quality ◽  
Expression Patterns ◽  
Rna Seq ◽  
Hub Genes ◽  
Hub Gene ◽  
Expression Of Genes ◽  
Multiple Dimensions ◽  
Cotton Species ◽  
Expression Bias

As two cultivated widely allotetraploid cotton species, although Gossypium hirsutum and Gossypium barbadense evolved from the same ancestor, they differ in fiber quality; the molecular mechanism of that difference should be deeply studied. Here, we performed RNA-seq of fiber samples from four G. hirsutum and three G. barbadense cultivars to compare their gene expression patterns on multiple dimensions. We found that 15.90–37.96% of differentially expressed genes showed biased expression toward the A or D subgenome. In particular, interspecific biased expression was exhibited by a total of 330 and 486 gene pairs at 10 days post-anthesis (DPA) and 20 DPA, respectively. Moreover, 6791 genes demonstrated temporal differences in expression, including 346 genes predominantly expressed at 10 DPA in G. hirsutum (TM-1) but postponed to 20 DPA in G. barbadense (Hai7124), and 367 genes predominantly expressed at 20 DPA in TM-1 but postponed to 25 DPA in Hai7124. These postponed genes mainly participated in carbohydrate metabolism, lipid metabolism, plant hormone signal transduction, and starch and sucrose metabolism. In addition, most of the co-expression network and hub genes involved in fiber development showed asymmetric expression between TM-1 and Hai7124, like three hub genes detected at 10 DPA in TM-1 but not until 25 DPA in Hai7124. Our study provides new insights into interspecific expression bias and postponed expression of genes associated with fiber quality, which are mainly tied to asymmetric hub gene network. This work will facilitate further research aimed at understanding the mechanisms underlying cotton fiber improvement.

scholarly journals Comparative mapping and correlation analysis revealed differentiation in the genetic basis of stem trichome development between cultivated tetraploid cotton species

2020 ◽  
Author(s):  
Rong Yuan ◽  
Yuefen Cao ◽  
Tengyu Li ◽  
Feng Yang ◽  
Li Yu ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Fiber Quality ◽  
Close Correlation ◽  
Tetraploid Cotton ◽  
Trichome Density ◽  
Cotton Species ◽  
Different Types ◽  
Morphological Index ◽  
Fiber Number ◽  
Modern Varieties

Abstract Background: Cotton stem trichomes and seed fibers are each single celled structures formed by protrusions of epidermal cells, and were found sharing the overlapping molecular mechanism . Compared with fibers, cotton stem trichomes are more easily observed, but the molecular mechanisms underlying their development are still poorly understood.Results: In this study, Gossypium hirsutum (Gh ) and G . barbadense ( Gb ) were found to differ greatly in percentages of varieties/accessions with glabrous stems and in trichome density, length, and number per trichopore. Gh varieties normally had long singular and clustered trichomes, while Gb varieties had short clustered trichomes. Genetic mapping using five F2 populations from crosses between glabrous varieties and those with different types of stem trichomes revealed that much variation among stem trichome phenotypes could be accounted for by different combinations of genes/alleles on Chr.06 and Chr.24. The twenty six F1 generations from crosses between varieties with different types of trichomes had varied phenotypes, further suggesting that the trichomes of tetraploid cotton were controlled by different genes/alleles. Compared to modern varieties, a greater proportion of Gh wild accessions were glabrous or had shorter and denser trichomes; whereas a smaller proportion of Gb primitive accessions had glabrous stems. A close correlation between fuzz fiber number and stem trichome density was observed in both Gh and Gb primitive accessions and modern varieties.Conclusion: Based on these findings, we hypothesize that stem trichomes evolved in parallel with seed fibers during the domestication of cultivated tetraploid cotton. In addition, the current results illustrated that stem trichome can be used as a morphological index of fiber quality in cotton conventional breeding.

Sign in / Sign up

Export Citation Format

Share Document