scholarly journals A Novel Function of the Lysophosphatidic Acid Receptor 3 (LPAR3) Gene in Zebrafish on Modulating Anxiety, Circadian Rhythm Locomotor Activity, and Short-Term Memory

2020 ◽  
Vol 21 (8) ◽  
pp. 2837 ◽  
Author(s):  
Yu-Nung Lin ◽  
Gilbert Audira ◽  
Nemi Malhotra ◽  
Nguyen Thi Ngoc Anh ◽  
Petrus Siregar ◽  
...  
Keyword(s):  
Circadian Rhythm ◽  
Social Interaction ◽  
Locomotor Activity ◽  
Lysophosphatidic Acid ◽  
Short Term Memory ◽  
Control Fish ◽  
Control Group ◽  
Memory Retention ◽  
Significant Difference ◽  
Lpa Receptors

Lysophosphatidic acid (LPA) is a small lysophospholipid molecule that activates multiple cellular functions through pathways with G-protein-coupled receptors. So far, six LPA receptors (LPAR1 to LPAR6) have been discovered and each one of them can connect to the downstream cell message-transmitting network. A previous study demonstrated that LPA receptors found in blood-producing stem cells can enhance erythropoietic processes through the activation of LPAR3. In the current study, newly discovered functions of LPAR3 were identified through extensive behavioral tests in lpar3 knockout (KO) zebrafish. It was found that the adult lpar3 KO zebrafish display an abnormal movement orientation and altered exploratory behavior compared to that of the control group in the three-dimensional locomotor and novel tank tests, respectively. Furthermore, consistent with those results, in the circadian rhythm locomotor activity test, the lpar3 KO zebrafish showed a lower level of angular velocity and average speed during the light cycles, indicating an hyperactivity-like behavior. In addition, the mutant fish also exhibited considerably higher locomotor activity during the dark cycle. Supporting those findings, this phenomenon was also displayed in the lpar3 KO zebrafish larvae. Furthermore, several important behavior alterations were also observed in the adult lpar3 KO fish, including a lower degree of aggression, less interest in conspecific social interaction, and looser shoal formation. However, there was no significant difference regarding the predator avoidance behavior between the mutant and the control fish. In addition, lpar3 KO zebrafish displayed memory deficiency in the passive avoidance test. These in vivo results support for the first time that the lpar3 gene plays a novel role in modulating behaviors of anxiety, aggression, social interaction, circadian rhythm locomotor activity, and memory retention in zebrafish.

P–372 Maternal circadian disruption is associated with impaired rhythmic expression of molecular clock genes and IUGR during placenta development in mice

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
N I Bektas ◽  
G Akcay ◽  
N Derin ◽  
D Adiguzel ◽  
C Celik-Ozenci
Keyword(s):  
Circadian Rhythm ◽  
Locomotor Activity ◽  
Molecular Clock ◽  
Clock Genes ◽  
Weight Ratio ◽  
Circadian Disruption ◽  
Control Group ◽  
Shift Workers ◽  
Rhythmic Expression ◽  
Registration Number

Abstract Study question Are molecular clock genes (MCGs) expressed rhythmically in mouse placenta, and whether maternal circadian rhythm disruption (MCRD) is associated with intrauterine growth retardation (IUGR) through disturbing rhythmic expression of MCGs? Summary answer Maternal circadian disruption causes impaired rhythmic expression of MCGs (Bmal1, Clock, Npas2, Per1, Per2, Per3, Cry1, and Cry2) and IUGR during placenta development in mice. What is known already The world economy is based on a 24/7 society and shift work or jet travel across time zones disrupts circadian rhythm in pregnant women. Evidence indicates that gestational chrono-disruption results in IUGR. Mature mouse and human placenta express MCGs. There is no information in the literature on whether the MCG expression in the placenta is rhythmic or not and whether the rhythmic expression of MCGs is impaired due to MCRD during pregnancy. Also, it is not known whether the association with MCRD and IUGR is related to MCGs. Study design, size, duration Young adult female BALB/c mice were paired with males until vaginal plug formation was verified. Females were randomly assigned to two groups: control and phase-advance. Controls remained on a constant 12-hr light:12-hr dark cycle, whereas phase-advanced mice were subjected to 6-hr advances in the LD cycle every 5 days. Placentae (n = 1329) and fetuses were obtained from 144 mice at Zeitgeber time (ZT)0, ZT6, ZT12, and ZT18 days 12, 14, and 16 of pregnancy. Participants/materials, setting, methods The following analysis was performed: (i) open field test was used for locomotor activity evaluations to confirm MCRD, (ii) placenta/fetus weight ratio for evaluation of IUGR development, (iii) morphometric evaluation of placental compartments utilizing H&E staining (iv) gene expression analysis of MCGs utilizing qRT-PCR. One-way and Two-way ANOVA test followed by Holm-Sidak posthoc test was used for multiple comparisons. Values are expressed as mean ± standard error, and values below p < 0.05 were considered statistically significant. Main results and the role of chance Expression of MCGs (Bmal1, Clock, Npas2, Per1, Per2, Per3, Cry1, and Cry2) was rhythmic in the early and mature placenta development stages (days 12, 14, 16). Locomotor activity tests reveal that the total distance covered on the 16th day of pregnancy significantly decreased compared to the control group (p = 0.000158). The ratio of the time spent in the outer/inner quadrant, an anxiety indicator, significantly increased in the MCRD group on the 14th (p = 0.0351) and 16th days of pregnancy (p = 0.000329). While the number of fetuses was similar in both groups for all gestational days (p = 0.896), in the MCRD group, the fetus/placenta weight ratio decreased significantly on the 12th and 16th days of pregnancy (p < 0.001). Thus, IUGR developed due to MCRD. Histomorphometry analysis of the placental compartments revealed a significant reduction in the spongiotrophoblast layer’s size on all days of pregnancy and the labyrinth layer on day 16 (p < 0.05). Finally, the rhythmic expression MCGs were impaired in placentas obtained from MCRD groups on days 12th, 14th, 6th of pregnancy (p < 0.001). In conclusion, we found a robust relationship with the disturbed MCGs expression and occurrence of IUGR during a chrono-disrupted gestation. Limitations, reasons for caution Since this study was conducted in mice, care should be taken when translating the results to humans. Wider implications of the findings: Our results in mice are important for initiating basic science knowledge regarding the outcomes of maternal chrono-disruption. Moreover, research in the placenta of gestational chrono-disrupted mothers, such as shift-workers, are urgently needed to translate our findings into the clinic. Trial registration number TUBITAK–119S121 and Akdeniz University Research Projects Unit TYL–2018–3960

scholarly journals GluD1 knockout mice with a pure C57BL/6N background show impaired fear memory, social interaction, and enhanced depressive-like behavior

2019 ◽  
Author(s):  
Chihiro Nakamoto ◽  
Meiko Kawamura ◽  
Ena Nakatsukasa ◽  
Rie Natsume ◽  
Keizo Takao ◽  
...  
Keyword(s):  
Social Interaction ◽  
Open Field ◽  
Forced Swim Test ◽  
Sensorimotor Gating ◽  
Fear Memory ◽  
Control Group ◽  
Behavioral Differences ◽  
Novelty Preference ◽  
Plus Maze ◽  
Significant Difference

AbstractThe GluD1 gene is associated with susceptibility for schizophrenia, autism, depression, and bipolar disorder. However, the function of GluD1 and how it is involved in these conditions remain elusive. In this study, we generated a GluD1-knockout (GluD1-KO) mouse line with a pure C57BL/6N genetic background and performed several behavioral analyses. Compared to a control group, GluD1-KO mice showed no significant anxiety-related behavioral differences, evaluated using behavior in an open field, elevated plus maze, a light-dark transition test, the resident-intruder test of aggression and sensorimotor gating evaluated by the prepulse inhibition test. However, GluD1-KO mice showed (1) hyper locomotor activity in the open field, (2) decreased sociability and social novelty preference in the three-chambered social interaction test, (3) impaired memory in contextual, but not cued fear conditioning tests, and (4) enhanced depressive-like behavior in a forced swim test. Pharmacological studies revealed that enhanced depressive-like behavior in GluD1-KO mice was restored by the serotonin reuptake inhibitors imipramine and fluoxetine, but not the norepinephrine transporter inhibitor desipramine. In addition, biochemical analysis revealed no significant difference in protein expression levels, such as other glutamate receptors in the synaptosome and postsynaptic densities prepared from the frontal cortex and the hippocampus. These results suggest that GluD1 plays critical roles in fear memory, sociability, and depressive-like behavior.

Biologging in combination with biotelemetry reveals behavior of Atlantic salmon following exposure to capture and handling stressors

2019 ◽  
Vol 76 (12) ◽  
pp. 2176-2183
Author(s):  
Robert J. Lennox ◽  
Jacqueline M. Chapman ◽  
William M. Twardek ◽  
Franziska Broell ◽  
Kristin Bøe ◽  
...  
Keyword(s):  
Locomotor Activity ◽  
Atlantic Salmon ◽  
Null Model ◽  
Control Fish ◽  
Control Group ◽  
Sample Population ◽  
Accelerometer Data ◽  
Air Exposure ◽  
Data Loggers ◽  
Total Body

We investigated the response of Atlantic salmon (Salmo salar) to capture and handling stressors by analyzing fine-scale locomotor activity using accelerometer data loggers and broader-scale movements by tracking migration with radiotelemetry. Half the sample population was exposed to experimental exercise and air exposure and released with a control group to simulate fisheries handling. All but two of the surviving fish (both in the treatment group) returned to the counting fence to resume the 2016 spawning migration (survival = 86%–91%). There were no differences in postrelease locomotor activity, measured by an index of total body action (jerk), between control and treatment salmon (p = 0.81). Comparison of mean time to return to the counting fence against a null model revealed that treatment salmon were significantly delayed in returning to the counting fence (p < 0.01), whereas control fish were not (p = 0.24). Both the abiotic environment and human interactions influenced locomotor activity of the migratory fish and synchrony of the migration with untreated conspecifics.

Effects of STM Span, Syntactic Awareness, and Vocabulary Knowledge on Iranian EFL Learners’ Reading Comprehension

2016 ◽  
Vol 6 (12) ◽  
pp. 2297
Author(s):  
Katayoun Hashemin ◽  
Asgar Mahmoudi
Keyword(s):  
Reading Comprehension ◽  
Quantitative Methods ◽  
Short Term Memory ◽  
Memory Span ◽  
Vocabulary Knowledge ◽  
Control Group ◽  
Syntactic Awareness ◽  
Efl Learners ◽  
Significant Difference ◽  
Knowledge Group

The present study aimed at investigating the effects of Short-Term Memory (STM) span, syntactic awareness, and vocabulary knowledge on Iranian EFL learners’ reading comprehension. The research data were collected using quantitative methods. The data collection instruments included Oxford Placement Test, a working memory span test, and reading pretests and posttests. Sixty seven female intermediate level students were randomly assigned into the three groups of control, syntactic awareness, and vocabulary knowledge. Their performances then were measured and analyzed. Not only did the results reveal that there was a significant difference between the experimental groups and the control group in reading comprehension but also a significant difference between the two experimental groups. In other words, students in the vocabulary knowledge group benefitted more than students in the syntactic awareness. In addition, findings from the study indicated that STM does not affect reading comprehension ability of the students.

scholarly journals Changes in intestinal microbiota and humoral immune response following probiotic administration in brown trout (Salmo trutta)

2007 ◽  
Vol 97 (3) ◽  
pp. 522-527 ◽  
Author(s):  
José Luis Balcázar ◽  
Ignacio de Blas ◽  
Imanol Ruiz-Zarzuela ◽  
Daniel Vendrell ◽  
Ana Cristina Calvo ◽  
...  
Keyword(s):  
Brown Trout ◽  
Salmo Trutta ◽  
Humoral Response ◽  
Lysozyme Activity ◽  
Control Fish ◽  
Control Group ◽  
Humoral Immune ◽  
Colony Forming Units ◽  
Significant Difference ◽  
Brown Trout Salmo Trutta

We studied the effect of several lactic acid bacteria (LAB) on the humoral response of brown trout (Salmo trutta). LAB groups (Lactococcus(Lc.)lactisssp.lactis,Lactobacillus(Lb.)sakeiandLeuconostoc(Leu.)mesenteroides) were administered orally at 106colony-forming units/g feed to brown trout for 2 weeks, after which fish were switched to an unsupplemented feed. Blood and intestinal samples were taken from the onset of feeding supplemented diets at 1, 2, 3 and 4 weeks. During the LAB-feeding period,Lc. lactisssp.lactis,Lb. sakeiandLeu. mesenteroidespersisted in the fish intestines, but the number of LAB slowly decreased in the intestines after changing to the unsupplemented diet. OnlyLb. lactisssp.lactisandLeu. mesenteroideswere detected at levels above 1 × 102colony-forming units/g at the end of the fourth week. In comparison to untreated control fish, the alternative complement activity in the serum was found to be significantly greater in all LAB groups at the end of the second week. Groups supplemented withLc. lactisssp.lactisandLeu. mesenteroidesexhibited an elevated level of lysozyme activity at the end of the third week, but the group supplemented withLb. sakeidid not exhibit any significant change in lysozyme activity. Serum immunoglobulin levels were higher compared with the control group, but there was no significant difference between the LAB and control groups.

scholarly journals COMPARISON OF PLYOMETRICS AND AEROBIC EXERCISES ON THE MEMORY

2021 ◽  
Vol 8 (2) ◽  
pp. 179-184
Author(s):  
Yohana Novelia Christin ◽  
Yuriz Bakhtiar ◽  
Hardian Hardian ◽  
Endang Kumaidah
Keyword(s):  
Cognitive Abilities ◽  
Short Term Memory ◽  
T Test ◽  
Control Group ◽  
P Value ◽  
Short Term ◽  
Term Memory ◽  
Significant Difference ◽  
Quasi Experimental ◽  
And Control

ABSTRACT Background: Problems of a person's desire to exercise is still a major highlight in the realm of health, both in Indonesia and in the world. Lack of exercise affects brain work, which is a smaller brain volume in people who are not actively exercising, so doing plyometrics and aerobic exercises can be a solution to these problems because exercise can be easily done for health and increased cognitive abilities. Aim: To understand which exercises are more effective at improving one's short-term memory. Methods: This study is a quasi-experimental study with 54 subjects, all of whom are students of the Faculty of Medicine Diponegoro University, aged between 15-25 years. Subjects were divided into three groups, namely the plyometrics group, the aerobic group and the control group. Subjects were selected by purposive sampling method and participated in the measurement of short-term memory at the time before exercise and after exercise for 6 weeks. Statistical analysis in this study using paired t-test and independent t-test. Results: There was a significant difference in the short-term memory measurement results between the treatment and control groups, but a higher increase was found in the plyometrics group, with p value <0.001. Conclusion: Plyometrics and aerobic exercises improve short-term memory in students of the Faculty of Medicine Diponegoro University. A more significant improvement was found in the plyometrics group. Keywords: plyometrics exercise, aerobic exercise, short term memory.

scholarly journals Anxiolytic and antidepressant effect of zinc on rats and its impact on general behavioural parameters

2013 ◽  
Vol 70 (4) ◽  
pp. 391-395 ◽  
Author(s):  
Janko Samardzic ◽  
Kristina Savic ◽  
Nemanja Stefanovic ◽  
Radomir Matunovic ◽  
Dragana Baltezarevic ◽  
...  
Keyword(s):  
Locomotor Activity ◽  
Forced Swim Test ◽  
Essential Element ◽  
Repeated Administration ◽  
Antidepressant Effect ◽  
Control Group ◽  
Single Application ◽  
Antidepressant Effects ◽  
Immobility Time ◽  
Significant Difference

Background/Aim. Zinc is an essential element which has considerable interaction with gamma-aminobutyric acid A type receptors (GABAA) and glutamate receptors in the central nervous system (CNS). It is believed that zinc acts as a potent inhibitor of glutamate N-methyl-D-aspartate (NMDA) receptors, and binding to structurally specific site on the GABAA receptor leads to inhibition of GABA dependent Cl-pass. The aim of our research was to test the anxiolytic and antidepressant effects of zinc after single application and its influence on general behavioural parameters after repeated administration. Methods. Male Wistar rats were treated with increasing doses of zinc histidine dehydrate (10, 20, 30 mg/kg, i.p.). To determine anxiolytic and antidepressant properties of zinc two models were used: elevated plus maze (EPM) and forced swim test (FST). Behavioural parameters (stillness and mobility) were, also, recorded after single and repeated administration of active substance. Results. Testing animals in the EPM showed a statistically significant difference as follows: dose of 20 mg/kg significantly increased the time animals spent in open arms, indicating an acute anxiolytic effect, while doses of 30 mg/kg significantly reduced the time in the open arms, indicating a potentially anxiogenic effect. Testing the animals by FST showed a statistically significant difference in immobility time of animals treated with the lowest applied (10 mg/kg) and highest applied (30 mg/kg) doses of zinc, compared to the control group. The first day of testing behavioral parameters showed the tendency to increase locomotor activity of the animals with the lowest dose of zinc (10 mg/kg), while the following day revealed a reduced activity with the highest dose applied (30 mg/kg). Conclusion. Zinc has important effects on the CNS: After single application, in all doses zinc showed antidepressant effects. The effects of zinc on anxiety and locomotor activity showed dose-dependent bidirectional effects.

Synergistic Effect of Freezing and Irradiation on Bonito Fish (Sarda sarda Bloch, 1793)

2016 ◽  
Vol 79 (12) ◽  
pp. 2136-2142 ◽  
Author(s):  
CAN OKAN ALTAN ◽  
HÜLYA TURAN
Keyword(s):  
Water Activity ◽  
Thiobarbituric Acid ◽  
Control Fish ◽  
Control Group ◽  
Total Volatile ◽  
Thiobarbituric Acid Reactive Substances ◽  
Total Volatile Basic Nitrogen ◽  
Basic Nitrogen ◽  
Significant Difference ◽  
Sarda Sarda

ABSTRACT In this study, bonito fish (Sarda sarda Bloch, 1793) were irradiated at 3 or 5 kGy, packaged, frozen, and stored at −20 ± 2°C for 12 months. During storage, the microbiological, physical, and chemical changes of the fish flesh were then assessed. Increasing the irradiation dose to 5 kGy provided greater inhibition of mesophilic and psychotropic aerobic bacteria (P &lt; 0.05). Because fewer bacteria were detected in irradiated (3 and 5 kGy) as compared with unirradiated control fish suggests that freezing and irradiation yielded better results when combined. Irradiation at 3 and 5 kGy also positively impacted water activity, total volatile basic nitrogen, thiobarbituric acid–reactive substances, trimethylamine, and odor compared with the control group. However, a significant difference was not seen between the 3- and 5-kGy groups in terms of water activity, total volatile basic nitrogen, thiobarbituric acid–reactive substances, trimethylamine, and odor results (P &gt; 0.05). Based on these findings, fish irradiated at 3 and 5 kGy remained within consumable limits during 12 months of frozen storage, while the control group was unacceptable after 9 months.

scholarly journals Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)

2013 ◽  
Vol 11 (1) ◽  
pp. 193-198 ◽  
Author(s):  
Celina Alcoforado Santos ◽  
Dominik Lenz ◽  
Geisamanda Pedrini Brandão ◽  
Adriana Regina Chippari-Gomes ◽  
Levy Carvalho Gomes
Keyword(s):  
Soluble Fraction ◽  
Micronucleus Test ◽  
Exposure Period ◽  
Diesel Oil ◽  
Water Soluble ◽  
Control Fish ◽  
Control Group ◽  
Water Soluble Fraction ◽  
Significant Difference ◽  
And Control

Diesel oil can be a source of contamination in aquatic environments, mainly as a result of spills. The effects of the water-soluble fraction of diesel (WSF) on Prochilodus vimboideswere assessed. Fish were exposed to three different WSF dilutions for up to 96 h and were compared to a control group. Damages in the fragments of DNA were analyzed using the Comet assay. The presence of erytrocyts abnormalities was assessed by micronucleus test. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and the accumulation of copper in gills were also analyzed. Fish exposed for 96 h had higher rates of damage than those exposed for 24 h. There was no significant difference regarding the presence of micronuclei between exposed and control fish and between 24-h and 96-h exposures. For AST, no significant difference was observed between samples collected at the two exposure times. Fish exposed to a 1:100 dilution of WSF showed higher activity of the enzyme ALT than the control fish after a 24-h exposure period. There was no bioaccumulation of copper in the gills. We conclude that the genotoxic effects of WSF in the cells are more evident in P. vimboides during an acute exposure.

Spatio-temporal Pattern of EEG in Young Brain Respiration-training Children

2001 ◽  
Vol 29 (01) ◽  
pp. 23-35 ◽  
Author(s):  
Hyung Rae Kim ◽  
Soo Yong Kim ◽  
Dai Jin Kim ◽  
Young Youn Kim ◽  
Sang Kyu Park ◽  
...  
Keyword(s):  
Short Term Memory ◽  
Brain Activity ◽  
Occipital Cortex ◽  
Temporal Analysis ◽  
High Amplitude ◽  
Control Group ◽  
Significant Difference ◽  
Spatio Temporal ◽  
Mental Integration ◽  
The Brain

We have evaluated the effect of 'Brain Respiration training on brain activity' using Karhunen-Loeve (KL) decomposition as a method for spatio-temporal analysis of the electroencepha logram (EEG). BR training is a form of breath-work to optimize the function of the brain by concentrating Qi energy in the brain. Recently, BR-training has been reported to improve emotional maturity (i.e., EQ), short-term memory and intuition (Yoo et al., 1998). EEG data were taken during BR-training from 12 young BR-trainees (average age: 9.4 years) who had trained fro 4 to 14 months, and during relaxation from age matched non-trained children. Spatio-temporal analysis showed a significant difference of EEG dynamics in right prefrontal, right inferior frontal, posterior temporal, parietal and occipital areas between BR-trainees and the control group. Amplitude of eigenvector components of BR-trainees in the areas of frontal, temporal and occipital cortex was larger than that of non-trained children (values were smaller in parietal cortex), with remarkably high amplitude alpha coherence all over the scalp. These results suggest that BR-taining possibly activates brain function through changes in the activity of the frontal association area where higher mental integration and creative activities are mediated.

Sign in / Sign up

Export Citation Format

Share Document