scholarly journals CaFtsH06, a Novel Filamentous Thermosensitive Protease Gene, Is Involved in Heat, Salt and Drought Stress Tolerance of Pepper (Capsicum annuum L.)

2021 ◽  
Vol 22 (13) ◽  
pp. 6953
Author(s):  
Jing-Jing Xiao ◽  
Rui-Xing Zhang ◽  
Abid Khan ◽  
Saeed ul Haq ◽  
Wen-Xian Gai ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Chlorophyll Content ◽  
Capsicum Annuum ◽  
Ectopic Expression ◽  
Protease Gene ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Capsicum Annuum L

Harsh environmental factors have continuous negative effects on plant growth and development, leading to metabolic disruption and reduced plant productivity and quality. However, filamentation temperature-sensitive H protease (FtsH) plays a prominent role in helping plants to cope with these negative impacts. In the current study, we examined the transcriptional regulation of the CaFtsH06 gene in the R9 thermo-tolerant pepper (Capsicum annuum L.) line. The results of qRT-PCR revealed that CaFtsH06 expression was rapidly induced by abiotic stress treatments, including heat, salt, and drought. The CaFtsH06 protein was localized to the mitochondria and cell membrane. Additionally, silencing CaFtsH06 increased the accumulation of malonaldehyde content, conductivity, hydrogen peroxide (H2O2) content, and the activity levels of superoxide dismutase and superoxide (·O2−), while total chlorophyll content decreased under these abiotic stresses. Furthermore, CaFtsH06 ectopic expression enhanced tolerance to heat, salt, and drought stresses, thus decreasing malondialdehyde, proline, H2O2, and ·O2− contents while superoxide dismutase activity and total chlorophyll content were increased in transgenic Arabidopsis. Similarly, the expression levels of other defense-related genes were much higher in the transgenic ectopic expression lines than WT plants. These results suggest that CaFtsH06 confers abiotic stress tolerance in peppers by interfering with the physiological indices through reducing the accumulation of reactive oxygen species, inducing the activities of stress-related enzymes and regulating the transcription of defense-related genes, among other mechanisms. The results of this study suggest that CaFtsH06 plays a very crucial role in the defense mechanisms of pepper plants to unfavorable environmental conditions and its regulatory network with other CaFtsH genes should be examined across variable environments.

scholarly journals Respuestas del crecimiento y el rendimiento en pimiento (Capsicum annuum L.) híbrido Nathalie a un lixiviado de vermicompost bovino

2020 ◽  
pp. 01
Author(s):  
José Lincoln Cedeño Guerra ◽  
Eduardo Fidel Héctor Ardisana ◽  
Antonio Torres García ◽  
Osvaldo Fosado Téllez
Keyword(s):  
Chlorophyll Content ◽  
Capsicum Annuum ◽  
Chemical Fertilizer ◽  
Growth And Yield ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Capsicum Annuum L ◽  
Palabras Clave ◽  
Experimental Treatments ◽  
Yield Responses

Growth and yield responses of pepper (Capsicum annuum L.) hybrid Nathalie to a bovine vermicompost leachate Resumen El pimiento (Capsicum annuum L.) es una hortaliza muy apreciada en el mundo por sus propiedades nutritivas, sus aplicaciones médicas y como condimento a los alimentos. En Ecuador se cultiva en varias provincias, y en todos los casos se utilizan altas cantidades de fertilizantes químicos sintéticos. Una opción es el empleo de bioestimulantes orgánicos que permiten producciones limpias. Este trabajo tuvo como objetivo investigar las respuestas del crecimiento y el rendimiento del pimiento híbrido Nathalie a la aplicación de un lixiviado de vermicompost bovino (LVCB). Se ensayaron seis tratamientos experimentales: suelo sin fertilizar, fertilizante químico YaraMilaTM ComplexTM, y tres diluciones de LVCB (1:10, 1:20 y 1:30 v/v). Las variables del crecimiento evaluadas fueron la altura de las plantas, el diámetro del tallo, la cantidad de hojas y el contenido de clorofilas totales a los 15, 30 y 45 días después del trasplante. Las variables del rendimiento (longitud, circunferencia y peso de los frutos) se midieron en cuatro cosechas. Se calculó el rendimiento estimado por hectárea a partir del peso total de los frutos de las cuatro cosechas y el área ocupada por las plantas muestreadas. No se observaron diferencias significativas en las variables del crecimiento y el contenido total de clorofilas entre los tratamientos experimentales. Los rendimientos estimados de todas las diluciones de LVCB fueron similares a los obtenidos con fertilizante químico y superaron al suelo sin fertilizar. Los resultados obtenidos sugieren que el uso de LVCB puede convertirse en una alternativa sostenible para la producción de pimiento sin contaminar el medio ambiente. Palabras clave: pimiento; Capsicum annuum L.; bioestimulantes. Abstract The pepper (Capsicum annuum L.) is a vegetable highly prized in the world for its nutritional properties, medical applications and as a condiment to food. In Ecuador it is grown in several provinces, and in all cases high amounts of synthetic chemical fertilizers are used. One option is the use of organic biostimulants that allow clean productions. This work aimed to investigate the growth and yield responses of the Nathalie hybrid pepper to the application of a leachate of bovine vermicompost (LVCB). Six experimental treatments were tested: unfertilized soil, YaraMilaTM ComplexTM chemical fertilizer, and three dilutions of LVCB (1:10, 1:20 and 1:30 v/v). The growth variables evaluated were the height of the plants, the diameter of the stem, the amount of leaves and the total chlorophyll content at 15, 30 and 45 days after transplantation. The yield variables (length, circumference and weight of the fruits) were measured in four crops. The estimated yield per hectare was calculated from the total weight of the fruits of the four crops and the area occupied by the sampled plants. No significant differences were observed in the growth variables and the total chlorophyll content between the experimental treatments. The estimated yields of all dilutions of LVCB were similar to those obtained with chemical fertilizer and exceeded the soil without fertilizing. The results obtained suggest that the use of LVCB can become a sustainable alternative for pepper production without polluting the environment. Keywords: pepper; Capsicum annuum L.; bioestimulants.

scholarly journals Respuestas de crecimiento, contenido de clorofila y rendimiento a la aplicación de lixiviado de vermicompost de estiércol bovino en el pimiento (Capsicum annuum L. híbrido Quetzal)

2020 ◽  
pp. 11
Author(s):  
Cristhian Josue Cedeño Solorzano ◽  
Antonio Torres García ◽  
Eduardo Fidel Héctor Ardisana
Keyword(s):  
Chlorophyll Content ◽  
Plant Height ◽  
Capsicum Annuum ◽  
Foliar Application ◽  
Economic Value ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Capsicum Annuum L ◽  
Chemical Fertilization ◽  
Vermicompost Leachate

Growth, chlorophyll content and yield responses after application of bovine manure vermicompost leachate in pepper (Capsicum annuum L. Quetzal hybrid) Resumen El pimiento (Capsicum annuum L.) está presente en la dieta alimenticia de numerosas personas y tiene un gran valor económico para los agricultores que se dedican al cultivo de esta hortaliza. La presente investigación se realizó en la Facultad de Ingeniería Agronómica de la Universidad Técnica de Manabí, en el campus experimental La Teodomira. Se evaluó el comportamiento del híbrido de pimiento Quetzal F1 en cinco tratamientos: la aplicación foliar de tres diluciones (1:10, 1:20 y 1:30 v/v) de lixiviado de vermicompost de estiércol bovino (LVEB), fertilización química y suelo sin fertilización. Las variables evaluadas fueron la altura de la planta, el diámetro del tallo, la cantidad de hojas, el contenido de clorofilas totales y el peso de los frutos. A partir de esta última se estimó el rendimiento por hectárea. En general, los valores de todas las variables fueron significativamente superiores en las plantas que fueron tratadas con las diluciones de LVEB con respecto a las que crecieron en suelo sin fertilizar o con fertilización química. La dilución 1:30 del LVEB se destacó por encima de las restantes, mostrando incrementos significativamente mayores que la fertilización química en la altura de planta, el diámetro del tallo, la cantidad de hojas, el contenido de clorofilas totales, y aumentando en 1,28 tha-1 el rendimiento en comparación con las plantas que recibieron fertilizante químico. Los resultados demuestran los efectos benéficos que ejerce el lixiviado de vermicompost de estiércol bovino en el pimiento y potencian a este bioestimulante como alternativa sostenible en la producción hortícola.  Palabras clave: pimiento; lixiviados; vermicompost; bioestimulantes   Abstract The pepper (Capsicum annuum L.) is present in the diet of many people and has great economic value for farmers who are dedicated to the cultivation of this vegetable. This research was carried out at the Faculty of Agronomic Engineering of the Technical University of Manabí, on the La Teodomira experimental campus. The behavior of the Quetzal F1 pepper hybrid was evaluated under five treatments: foliar application of three dilutions (1:10, 1:20 and 1:30 v/v) of bovine manure vermicompost leachate (LVEB), chemical fertilization and soil without fertilization. The variables evaluated were the plant height, the diameter of the stem, the number of leaves, the total chlorophyll content and the weight of the fruits. From the latter, the yield per hectare was estimated. In general, the values ​​of all the variables were significantly higher in the plants that were treated with the dilutions of LVEB with respect to those that grew in soil without fertilizing or with chemical fertilization. The 1:30 dilution of the LVEB stood out above the remaining ones, showing significantly greater increases than chemical fertilization for plant height, stem diameter, leaf quantity, total chlorophyll content, and increasing yield by 1,28 tha-1 compared to plants that received chemical fertilizer. The results demonstrate the beneficial effects exerted by the bovine manure vermicompost leachate in pepper and enhance this biostimulant as a sustainable alternative in horticultural production. Keywords: pepper; leachates; vermicompost; biostimulants.

Ectopic expression of a fruit phytoene synthase from Citrus paradisi Macf. promotes abiotic stress tolerance in transgenic tobacco

2012 ◽  
Vol 39 (12) ◽  
pp. 10201-10209 ◽  
Author(s):  
Luciana C. Cidade ◽  
Tahise M. de Oliveira ◽  
Amanda F. S. Mendes ◽  
Amanda F. Macedo ◽  
Eny I. S. Floh ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Abiotic Stress Tolerance ◽  
Ectopic Expression ◽  
Phytoene Synthase ◽  
Citrus Paradisi

scholarly journals Improving the Keeping Quality and Vase Life of Cut Alstroemeria Flowers by Pre and Post-harvest Salicylic Acid Treatments

2013 ◽  
Vol 5 (3) ◽  
pp. 364-370 ◽  
Author(s):  
Elnaz SOLEIMANY-FARD ◽  
Khodayar HEMMATI ◽  
Ahmad KHALIGHI
Keyword(s):  
Salicylic Acid ◽  
Water Balance ◽  
Chlorophyll Content ◽  
Water Loss ◽  
Vase Life ◽  
Total Chlorophyll ◽  
Cut Flowers ◽  
Fresh Weight ◽  
Total Chlorophyll Content ◽  
Keeping Quality

Keeping quality and length of vase life are important factors for evaluation of cut flowers quality, for both domestic and export markets. Studding the effect of pre- and post-harvest salicylic acid applications on keeping quality and vase life of cut alstroemeria flowers during vase period is the approach taken. Aqueous solutions of salicylic acid at 0.0 (with distilled water), 1, 2 and 3 mM were sprayed to run-off (approximately 500 mL per plant), about two weeks before flowers harvest. The cut flowers were harvested in the early morning and both of cut flowers treated (sprayed) and untreated were kept in vase solutions containing salicylic acid at 0.0 (with distilled water), 1, 2 and 3 mM. Sucrose at 4% was added to all treatments as a base solution. The changes in relative fresh weight, water uptake, water loss, water balance, total chlorophyll content and vase life were estimated during vase period. The results showed that the relative fresh weight, water uptake, water balance, total chlorophyll content and vase life decreased significantly while the water loss increased significantly during experiment for all treatments. A significant difference between salicylic acid and control treatments in all measured parameters is observed. During vase period, the salicylic acid treatments maintained significantly a more favourable relative fresh weight, water uptake, water balance, total chlorophyll content and supressed significantly water loss, as compared to control treatment. Also, the results showed that the using salicylic acid increased significantly the vase life cut alstroemeria flowers, over control. The highest values of measured parameters were found when plants were treated by pre + post-harvest application of salicylic acid at 3 mM. The result revealed that the quality attributes and vase life of cut alstroemeria flowers were improved by the use of salicylic acid treatment.

Ectopic expression of glycosyltransferase UGT76E11 increases flavonoid accumulation and enhances abiotic stress tolerance in Arabidopsis

Plant Biology ◽  
2017 ◽  
Vol 20 (1) ◽  
pp. 10-19 ◽  
Author(s):  
Qin Li ◽  
Hui-Min Yu ◽  
Xia-Fei Meng ◽  
Ji-Shan Lin ◽  
Yan-Jie Li ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Ectopic Expression ◽  
Flavonoid Accumulation

scholarly journals Antioxidant gene expression, chlorophyll, and starch content in Persian lime (Citrus latifolia Tanaka Ex Q. Jiménez) trees with HLB by application of elicitors of plant resistance

2021 ◽  
Vol 6 (2) ◽  
pp. 86-102
Author(s):  
Felipe Roberto Flores-de la Rosa ◽  
◽  
Ricardo Santillán-Mendoza ◽  
Cynthia Guadalupe Rodríguez-Quibrera ◽  
Antonio Martínez-Ruiz ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Starch Content ◽  
Starch Accumulation ◽  
Economic Losses ◽  
Total Chlorophyll ◽  
Gamma Aminobutyric Acid ◽  
Antioxidant Genes ◽  
Spectrophotometric Methods ◽  
Total Chlorophyll Content ◽  
Randomized Design

Persian lime is economically important for Mexico. However, the disease known as Huanglongbing (HLB) causes chlorosis symptoms in the foliage that affect the growth and decreases the yield of the trees. The expression of chlorosis symptoms is associated with starch accumulation and inhibition of antioxidant activity. The objective of the present work was to determine the effect of the application of three resistance elicitors on the expression of three antioxidant genes and on starch and chlorophyll content in Persian lime trees with HLB. Salicylic acid (SA), jasmonic acid (JA) or gamma-aminobutyric acid (GABA) were applied every eight days for nine weeks using a completely randomized design and a one-way ANOVA was performed for the analysis. Expression of APX, CAT and SOD genes was quantified at week nine at four different times. Starch and total chlorophyll content was estimated every three weeks by spectrophotometric methods. The application of the elicitors significantly increased the expression of the three genes, with SA and GABA generating the greatest increase at different times of application. No difference was found in starch and total chlorophyll content at most of the times evaluated. The use of resistance elicitors is promising in the management of HLB in plots already infected, seeking to extend the productive life of the orchards and thus counteract the economic losses caused by HLB.

scholarly journals PENGARUH PERBANDINGAN IKAN KEMBUNG (Rastrelliger kanagurta) DENGAN SAYUR GONDA (Sphenoclea zeylanica Gaertner) TERHADAP KARAKTERISTIK BAKSO

2020 ◽  
Vol 9 (4) ◽  
pp. 426
Author(s):  
Brogina Mayank Dini ◽  
Luh Putu Trisna Darmayanti ◽  
I Ketut Suter
Keyword(s):  
Antioxidant Capacity ◽  
Protein Content ◽  
Water Content ◽  
Chlorophyll Content ◽  
Fat Content ◽  
Ash Content ◽  
Carbohydrate Content ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Randomized Design

This research was conducted with the aim to determine the effect of comparison of mackerel with gonda vegetable on meatballs characteristics and get a comparison of mackerel with specific gonda vegetable that can produce meatballs with the best characteristics. This study uses a Completely Randomized Design (CRD) with treatment that is the comparison of mackerel with gonda vegetable consisting of 6 levels: 100%: 0%; 95%: 5%; 90%: 10%; 85%: 15%; 80%: 20%; 75%: 25%. Each treatment was repeated 3 times so that obtained 18 units. The data is then analyzed by analysis of variance and if the treatment influences the proposed variable then it is continued by Duncan. The results showed that the comparison of mackerel and gonda vegetable had a very significant effect on water content, ash content, fat content, protein content, carbohydrate content, total chlorophyll content, antioxidant capacity, color (scoring), texture, aroma, overall acceptance and no significant effect for texture, aroma and taste. Comparison of 80% mackerel and 20% gonda vegetables had the best characteristics, with 62.99% water content, 1.56% ash content, 1.28% fat content, 8.75% protein content, 25.43% carbohydrate content, 18.39% total chlorophyll content, antioxidant capacity 25.63%. Sensory properties obtained are color (rather green, rather like), texture (rather like), flavor (liked) and overall acceptance (like).

scholarly journals The Effect of Organic Fertilizers on Growth and Yield of Water Spinach (Ipomoea reptans Poir)

2021 ◽  
Vol 3 (2) ◽  
pp. 37-44
Author(s):  
Selvia Dewi Pohan
Keyword(s):  
Plant Growth ◽  
Water Content ◽  
Chlorophyll Content ◽  
Organic Fertilizers ◽  
Chicken Manure ◽  
Growth And Yield ◽  
Total Chlorophyll ◽  
Cow Manure ◽  
Water Spinach ◽  
Total Chlorophyll Content

Water spinach (Ipomoea reptans Poir) had been identified as a nutritious vegetable with high demand in Indonesia. Besides, this plant also has been evidenced to play an important role in environmental cleaning as phytoremediator. The study about the effect of organic fertilizers on the growth and yield of water spinach (Ipomoea reptans Poir) has been conducted in the Green House of Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan. The study aims to investigate the most effective type of fertilizers and the dose for water spinach’s (Ipomoea reptans Poir) growth and yield. A Completely Randomized Factorial Design was designed for the experiment with two factors and three repetitions. The first factor is the type of fertilizer (chicken manure, cow manure, and compost), and the second factor is the dose of the fertilizer (1:1, 2:1, and 3:1). The parameters such as plant height, number of shoots, number of leaves, fresh weight, dry weight, leaf’s total chlorophyll content, and water content were measured to evaluate plant growth and yield. The General Linear Model used SPSS 21 programs was applied to analyze the collected data. Study results revealed that cow manure increased plant growth and yield significantly with dose 2:1 as the finest treatment, followed by dose 3:1. Cow manure also increased total chlorophyll content (8.0574c mg. L-1), with the most suitable dose was 2:1 (8.2807 mg. L-1). The plant’s water content tended to be high in chicken manure (93%), and the lower water content was in cow manure with dose 3:1 (87.5%).

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