scholarly journals Furnishing Wound Repair by the Subcutaneous Fascia

2021 ◽  
Vol 22 (16) ◽  
pp. 9006
Author(s):  
Dongsheng Jiang ◽  
Yuval Rinkevich
Keyword(s):  
Wound Healing ◽  
Connective Tissue ◽  
Wound Repair ◽  
Cell Types ◽  
Superficial Fascia ◽  
Connective Tissue Layer ◽  
Tissue Contraction ◽  
Rheologic Properties ◽  
Chronic Skin ◽  
Novel Therapeutic Strategies

Mammals rapidly heal wounds through fibrous connective tissue build up and tissue contraction. Recent findings from mouse attribute wound healing to physical mobilization of a fibroelastic connective tissue layer that resides beneath the skin, termed subcutaneous fascia or superficial fascia, into sites of injury. Fascial mobilization assembles diverse cell types and matrix components needed for rapid wound repair. These observations suggest that the factors directly affecting fascial mobility are responsible for chronic skin wounds and excessive skin scarring. In this review, we discuss the link between the fascia’s unique tissue anatomy, composition, biomechanical, and rheologic properties to its ability to mobilize its tissue assemblage. Fascia is thus at the forefront of tissue pathology and a better understanding of how it is mobilized may crystallize our view of wound healing alterations during aging, diabetes, and fibrous disease and create novel therapeutic strategies for wound repair.

scholarly journals Adipose-derived stromal/stem cells improve epidermal homeostasis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mariko Moriyama ◽  
Shunya Sahara ◽  
Kaori Zaiki ◽  
Ayumi Ueno ◽  
Koichi Nakaoji ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Culture System ◽  
Wound Repair ◽  
Cell Types ◽  
Skin Wound ◽  
Epidermal Keratinocytes ◽  
Stromal Stem Cells ◽  
Collagen Vitrigel

AbstractWound healing is regulated by complex interactions between the keratinocytes and other cell types including fibroblasts. Recently, adipose-derived mesenchymal stromal/stem cells (ASCs) have been reported to influence wound healing positively via paracrine involvement. However, their roles in keratinocytes are still obscure. Therefore, investigation of the precise effects of ASCs on keratinocytes in an in vitro culture system is required. Our recent data indicate that the epidermal equivalents became thicker on a collagen vitrigel membrane co-cultured with human ASCs (hASCs). Co-culturing the human primary epidermal keratinocytes (HPEK) with hASCs on a collagen vitrigel membrane enhanced their abilities for cell proliferation and adhesion to the membrane but suppressed their differentiation suggesting that hASCs could maintain the undifferentiated status of HPEK. Contrarily, the effects of co-culture using polyethylene terephthalate or polycarbonate membranes for HPEK were completely opposite. These differences may depend on the protein permeability and/or structure of the membrane. Taken together, our data demonstrate that hASCs could be used as a substitute for fibroblasts in skin wound repair, aesthetic medicine, or tissue engineering. It is also important to note that a co-culture system using the collagen vitrigel membrane allows better understanding of the interactions between the keratinocytes and ASCs.

scholarly journals Platelet-Therapeutics to Improve Tissue Regeneration and Wound Healing—Physiological Background and Methods of Preparation

Biomedicines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 869
Author(s):  
Ellen E. Jansen ◽  
Andreas Braun ◽  
Patrick Jansen ◽  
Matthias Hartmann
Keyword(s):  
Wound Healing ◽  
Tissue Repair ◽  
Wound Repair ◽  
Cell Types ◽  
Therapeutic Use ◽  
Cellular Mechanisms ◽  
Platelet Rich Fibrin ◽  
Primary Hemostasis ◽  
Frequent Use ◽  
Extracellular Structures

Besides their function in primary hemostasis, platelets are critically involved in the physiological steps leading to wound healing and tissue repair. For this purpose, platelets have a complex set of receptors allowing the recognition, binding, and manipulation of extracellular structures and the detection of pathogens and tissue damage. Intracellular vesicles contain a huge set of mediators that can be released to the extracellular space to coordinate the action of platelets as other cell types for tissue repair. Therapeutically, the most frequent use of platelets is the intravenous application of platelet concentrates in case of thrombocytopenia or thrombocytopathy. However, there is increasing evidence that the local application of platelet-rich concentrates and platelet-rich fibrin can improve wound healing and tissue repair in various settings in medicine and dentistry. For the therapeutic use of platelets in wound healing, several preparations are available in clinical practice. In the present study we discuss the physiology and the cellular mechanisms of platelets in hemostasis and wound repair, the methods used for the preparation of platelet-rich concentrates and platelet-rich fibrin, and highlight some examples of the therapeutic use in medicine and dentistry.

scholarly journals The Role of Immune Cells and Cytokines in Intestinal Wound Healing

2019 ◽  
Vol 20 (23) ◽  
pp. 6097 ◽  
Author(s):  
Xiang Xue ◽  
Daniel M. Falcon
Keyword(s):  
Wound Healing ◽  
Immune Cells ◽  
Wound Repair ◽  
Immune Cell ◽  
Cell Types ◽  
Repair Process ◽  
Interleukin 10 ◽  
Lymphoid Cells ◽  
Necrosis Factor Alpha ◽  
Interleukin 22

Intestinal wound healing is a complicated process that not only involves epithelial cells but also immune cells. In this brief review, we will focus on discussing the contribution and regulation of four major immune cell types (neutrophils, macrophages, regulatory T cells, and innate lymphoid cells) and four cytokines (interleukin-10, tumor necrosis factor alpha, interleukin-6, and interleukin-22) to the wound repair process in the gut. Better understanding of these immune factors will be important for developing novel targeted therapy.

Skin repair and scar formation: the central role of TGF-β

2003 ◽  
Vol 5 (8) ◽  
pp. 1-22 ◽  
Author(s):  
Steven R. Beanes ◽  
Catherine Dang ◽  
Chia Soo ◽  
Kang Ting
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Cell Types ◽  
Impaired Wound Healing ◽  
Clinical Scenarios ◽  
Β Function ◽  
Diabetic Wounds

Wound healing is a complex process that we have only recently begun to understand. Central to wound repair is transforming growth factor β (TGF-β), a cytokine secreted by several different cell types involved in healing. TGF-β has diverse effects, depending upon the tissue studied. This review focuses on healing in skin, particularly the phases of cutaneous wound repair and the role of TGF-β in normal and impaired wound-healing models. It also explores TGF-β activity in scarless foetal wound healing. Knowledge of TGF-β function in scarless repair is critical to improving healing in clinical scenarios, such as diabetic wounds and hypertrophic scars.

scholarly journals In vitro wound healing assays – state of the art

2016 ◽  
Vol 17 (1-2) ◽  
Author(s):  
Anne Stamm ◽  
Kerstin Reimers ◽  
Sarah Strauß ◽  
Peter Vogt ◽  
Thomas Scheper ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Molecular Mechanisms ◽  
Healing Process ◽  
Cell Monolayer ◽  
3D Culture ◽  
Cell Types ◽  
In Vitro Assays ◽  
Complex Wound

AbstractWound healing is essential for the restoration of the barrier function of the skin. During this process, cells at the wound edges proliferate and migrate, leading to re-epithelialization of the wound surface. Wound healing assays are used to study the molecular mechanisms of wound repair, as well as in the investigation of potential therapeutics and treatments for improved healing. Numerous models of wound healing have been developed in recent years. In this review, we focus on in vitro assays, as they allow a fast, cost-efficient and ethical alternative to animal models. This paper gives a general overview of 2-dimensional (2D) cell monolayer assays by providing a description of injury methods, as well as an evaluation of each assay’s strengths and limitations. We include a section reviewing assays performed in 3-dimensional (3D) culture, which employ bioengineered skin models to capture complex wound healing mechanics like cell-matrix interactions and the interplay of different cell types in the healing process. Finally, we discuss in detail available software tools and algorithms for data analysis.

scholarly journals B2 agonist (Salbutamol) modulate skin wound healing processes

2018 ◽  
Vol 9 (1) ◽  
pp. 37
Author(s):  
Fahim M. Mahmood ◽  
Hayder B. Sahib ◽  
Khalid W. Qassim
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Cell Types ◽  
Skin Wound ◽  
Control Group ◽  
Related Factors ◽  
Skin Wound Healing ◽  
Wound Model ◽  
Numerous Cell

Wound healing is a complex physiological and dynamic process required the coordination of numerous cell types and biological processes to regenerate damaged tissue and initiate repair which is dependent on a number of inter-related factors. This study was aimed to demonstrate whether the ?2 receptor has role in wound healing and angiogenesis. A murine wild-type (in vivo), excisional skin wound model was done to demonstrate that activation of ?2AR delay wound repair, twenty-four male albino mice were used to investigate the effect of the drug on experimental wound healing grossly, histo-pathologically and immune-histochemically compared with vehicle-only controls. The results showed that the rate of wound healing was significantly slower in salbutamol group than in control group (P

scholarly journals Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

2015 ◽  
Vol 36 (1) ◽  
pp. 1-23 ◽  
Author(s):  
Bizunesh M. Borena ◽  
Ann Martens ◽  
Sarah Y. Broeckx ◽  
Evelyne Meyer ◽  
Koen Chiers ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Growth Factor ◽  
Wound Repair ◽  
Cell Types ◽  
Skin Wound ◽  
Wound Management ◽  
Skin Wound Healing

Mammal skin has a crucial function in several life-preserving processes such as hydration, protection against chemicals and pathogens, initialization of vitamin D synthesis, excretion and heat regulation. Severe damage of the skin may therefore be life-threatening. Skin wound repair is a multiphased, yet well-orchestrated process including the interaction of various cell types, growth factors and cytokines aiming at closure of the skin and preferably resulting in tissue repair. Regardless various therapeutic modalities targeting at enhancing wound healing, the development of novel approaches for this pathology remains a clinical challenge. The time-consuming conservative wound management is mainly restricted to wound repair rather than restitution of the tissue integrity (the so-called “restitutio ad integrum”). Therefore, there is a continued search towards more efficacious wound therapies to reduce health care burden, provide patients with long-term relief and ultimately scarless wound healing. Recent in vivo and in vitro studies on the use of skin wound regenerative therapies provide encouraging results, but more protracted studies will have to determine whether the effect of observed effects are clinically significant and whether regeneration rather than repair can be achieved. For all the aforementioned reasons, this article reviews the emerging field of regenerative skin wound healing in mammals with particular emphasis on growth factor- and stem cell-based therapies.

Collagen Remodeling in Wound Healing by Gingival Fibroblasts in Vitro

1988 ◽  
Vol 2 (2) ◽  
pp. 228-233 ◽  
Author(s):  
T. Yajima
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Experimental System ◽  
Cell Types ◽  
Ultrastructural Level ◽  
Gingival Fibroblasts ◽  
Collagen Remodeling ◽  
Cytochemical Demonstration ◽  
Tracer Experiments

Collagen degradation by fibroblasts was studied in the absence of other cell types to improve our understanding of the mechanisms by which fibroblasts digest collagen. Human gingival fibroblasts were cultured in α -MEM medium for eight weeks. Incisional wounds were made in the fibroblast cultures, and the cells were fixed by different procedures at two days post-wounding. Collagen remodeling has been investigated by tracer experiments and by cytochemical demonstration of acid and alkaline phosphatase activity at the ultrastructural level and stereological analysis in experimental wound-healing in vitro. The results showed that fibroblasts in the wounded zone exhibited high collagen phagocytic activity, and indicate that fibroblasts have a fundamental role to play in collagen remodeling in wound repair in vitro. This in vitro experimental system is also suggested as a useful model for the analysis of collagen remodeling in wound-healing by fibroblasts.

scholarly journals A pulsatile release platform based on photo-induced imine-crosslinking hydrogel promotes scarless wound healing

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jian Zhang ◽  
Yongjun Zheng ◽  
Jimmy Lee ◽  
Jieyu Hua ◽  
Shilong Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Skin Wound ◽  
Large Animal ◽  
Skin Wounds ◽  
Tgfβ Signaling ◽  
Pulsatile Release ◽  
Ecm Extracellular Matrix

AbstractEffective healing of skin wounds is essential for our survival. Although skin has strong regenerative potential, dysfunctional and disfiguring scars can result from aberrant wound repair. Skin scarring involves excessive deposition and misalignment of ECM (extracellular matrix), increased cellularity, and chronic inflammation. Transforming growth factor-β (TGFβ) signaling exerts pleiotropic effects on wound healing by regulating cell proliferation, migration, ECM production, and the immune response. Although blocking TGFβ signaling can reduce tissue fibrosis and scarring, systemic inhibition of TGFβ can lead to significant side effects and inhibit wound re-epithelization. In this study, we develop a wound dressing material based on an integrated photo-crosslinking strategy and a microcapsule platform with pulsatile release of TGF-β inhibitor to achieve spatiotemporal specificity for skin wounds. The material enhances skin wound closure while effectively suppressing scar formation in murine skin wounds and large animal preclinical models. Our study presents a strategy for scarless wound repair.

Current knowledge of immunomodulation strategies for chronic skin wound repair

2021 ◽  
Author(s):  
Parisa Heydari ◽  
Mahshid Kharaziha ◽  
Jaleh Varshosaz ◽  
Shaghayegh Haghjooy Javanmard
Keyword(s):  
Wound Repair ◽  
Current Knowledge ◽  
Skin Wound ◽  
Chronic Skin
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