scholarly journals Effect of Melt-Derived Bioactive Glass Particles on the Properties of Chitosan Scaffolds

2019 ◽  
Vol 10 (3) ◽  
pp. 38 ◽  
Author(s):  
Hamasa Faqhiri ◽  
Markus Hannula ◽  
Minna Kellomäki ◽  
Maria Teresa Calejo ◽  
Jonathan Massera
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bioactive Glass ◽  
Pore Size ◽  
Glass Content ◽  
Micro Computed Tomography ◽  
Glass Composite ◽  
Composite Scaffolds ◽  
Bioactive Glasses

This study reports on the processing of three-dimensional (3D) chitosan/bioactive glass composite scaffolds. On the one hand, chitosan, as a natural polymer, has suitable properties for tissue engineering applications but lacks bioactivity. On the other hand, bioactive glasses are known to be bioactive and to promote a higher level of bone formation than any other biomaterial type. However, bioactive glasses are hard, brittle, and cannot be shaped easily. Therefore, in the past years, researchers have focused on the processing of new composites. Difficulties in reaching composite materials made of polymer (synthetic or natural) and bioactive glass include: (i) The high glass density, often resulting in glass segregation, and (ii) the fast bioactive glass reaction when exposed to moisture, leading to changes in the glass reactivity and/or change in the polymeric matrix. Samples were prepared with 5, 15, and 30 wt% of bioactive glass S53P4 (BonAlive ®), as confirmed using thermogravimetric analysis. MicrO–Computed tomography and optical microscopy revealed a flaky structure with porosity over 80%. The pore size decreased when increasing the glass content up to 15 wt%, but increased back when the glass content was 30 wt%. Similarly, the mechanical properties (in compression) of the scaffolds increased for glass content up to 15%, but decreased at higher loading. Ions released from the scaffolds were found to lead to precipitation of a calcium phosphate reactive layer at the scaffold surface. This is a first indication of the potential bioactivity of these materials. Overall, chitosan/bioactive glass composite scaffolds were successfully produced with pore size, machinability, and ability to promote a calcium phosphate layer, showing promise for bone tissue engineering and the mechanical properties can justify their use in non-load bearing applications.

In vitro and in vivo bone formation potential of surface calcium phosphate-coated polycaprolactone and polycaprolactone/bioactive glass composite scaffolds

2016 ◽  
Vol 30 ◽  
pp. 319-333 ◽  
Author(s):  
Patrina S.P. Poh ◽  
Dietmar W. Hutmacher ◽  
Boris M. Holzapfel ◽  
Anu K. Solanki ◽  
Molly M. Stevens ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bioactive Glass ◽  
Glass Composite ◽  
Composite Scaffolds ◽  
Formation Potential ◽  
In Vivo Bone Formation

Microstructural and Mechanical Properties of Polymer-Based Scaffolds Reinforced by Hydroxyapatite

2007 ◽  
Vol 544-545 ◽  
pp. 765-768 ◽  
Author(s):  
Hyeong Ho Jin ◽  
Won Ki Lee ◽  
Hong Chae Park ◽  
Seog Young Yoon
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Yield Strength ◽  
Pore Size ◽  
Compressive Modulus ◽  
Precipitation Method ◽  
Pore Morphology ◽  
Composite Scaffolds ◽  
Co Precipitation

Various polymer-based scaffolds reinforced by the hydroxyapatite (HAp) for bone tissue engineering were successfully synthesized by in-situ co-precipitation method. The influence of HAp in composite scaffolds on the pore morphology, microstructure, and mechanical properties was investigated. The polymer-based scaffolds appeared to be macroporous and an interconnected open pore microstructure with pore size around 200 μm. The pore structure of the composite scaffolds was not much changed by the presence of HAp but the pore size of the composite scaffolds decreased with adding the HAp. The compressive modulus and yield strength of the polymer-based scaffolds improved by the presence of HAp.

Preparation and properties of poly(ε-caprolactone)/bioactive glass nanofibre membranes for skin tissue engineering

2017 ◽  
Vol 33 (2) ◽  
pp. 195-209 ◽  
Author(s):  
Zefeng Lin ◽  
Wendong Gao ◽  
Limin Ma ◽  
Hong Xia ◽  
Weihan Xie ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bioactive Glass ◽  
Fibre Diameter ◽  
Skin Tissue ◽  
Glass Mass ◽  
Glass Composite ◽  
Elongation At Break ◽  
Skin Tissue Engineering ◽  
Electrospun Nanofibres

Poly(ε-caprolactone) composite nanofibres for skin tissue engineering and regeneration applications were prepared via electrospinning of poly(ε-caprolactone) nanofibres with bioactive glass nanoparticles at bioactive glass contents of 0, 2, 4, 6 and 8 wt%. The surface properties, water absorptivities, porosities, mechanical properties and biocompatibilities of the composite electrospun nanofibres were characterised in detail. Addition of bioactive glass improved the hydrophilicity and elastic modulus of membranes. The fibre diameter of the neat poly(ε-caprolactone) nanofibres was only 700 nm, but reinforcement with 2, 4, 6 and 8 wt% bioactive glass nanofibres increased the diameter to 1000, 1100, 900 and 800 nm, respectively. The minimum elongation at break of the bioactive glass–reinforced poly(ε-caprolactone) exceeded 100%, which indicated that the composite nanofibres had good mechanical properties. The porosities of the various nanofibres containing different mass loadings of bioactive glass all exceeded 90%. The best performance in terms of cell proliferation and adhesion was found when the bioactive glass mass percent reached 6 wt%. However, higher loadings were unfavourable for cell growth. These preliminary results indicate that poly(ε-caprolactone)/bioactive glass composite nanofibres have promise for skin tissue engineering applications.

scholarly journals A Comparative Evaluation of the Mechanical Properties of Two Calcium Phosphate/Collagen Composite Materials and Their Osteogenic Effects on Adipose-Derived Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Qing Li ◽  
Tong Wang ◽  
Gui-feng Zhang ◽  
Xin Yu ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Composite Materials ◽  
Calcium Phosphate ◽  
Tricalcium Phosphate ◽  
Comparative Evaluation ◽  
Bone Defects ◽  
Adipose Derived Stem Cells ◽  
Composite Scaffolds

Adipose-derived stem cells (ADSCs) are ideal seed cells for use in bone tissue engineering and they have many advantages over other stem cells. In this study, two kinds of calcium phosphate/collagen composite scaffolds were prepared and their effects on the proliferation and osteogenic differentiation of ADSCs were investigated. The hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) composite scaffolds (HTPSs), which have an additionalβ-tricalcium phosphate, resulted in better proliferation of ADSCs and showed osteogenesis-promoting effects. Therefore, such composite scaffolds, in combination with ADSCs or on their own, would be promising for use in bone regeneration and potential clinical therapy for bone defects.

Preparation and characterization of highly porous ceramic-based nanocomposite scaffolds with improved mechanical properties using the liquid phase-assisted sintering method

2018 ◽  
Vol 233 (9) ◽  
pp. 1854-1865
Author(s):  
Mansure Kazemi ◽  
Bahareh Nazari ◽  
Jafar Ai ◽  
Nasrin Lotfibakhshaiesh ◽  
Ali Samadikuchaksaraei ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Culture ◽  
Bioactive Glass ◽  
Tricalcium Phosphate ◽  
Porous Ceramic ◽  
Simulated Body Fluid Solution ◽  
Biological Properties ◽  
Glass Content ◽  
Composite Scaffolds ◽  
Highly Porous

Recent advances in the field of biomaterials have led to the development of ceramic–matrix nanocomposites with enhanced mechanical properties, which is essential for hard tissue scaffolds. In this study, the improvement in mechanical and biological properties of β-tricalcium phosphate reinforced with 45S5 bioactive glass under different sintering conditions was studied. In order to improve the thermal stability and biological responses, β-tricalcium phosphate was doped with 5 mol% strontium ions. Highly porous nanocomposites, with different weight ratios of Sr-tricalcium phosphate/bioactive glass (75/25, 50/50, 25/75), were fabricated through the foam replication method by sintering samples under various thermal conditions (1200–1250 ℃/0–1 h). The effects of bioactive glass content and sintering parameters on microstructure and mechanical behaviors of the nanocomposites were assessed. The obtained results showed that increasing 45S5 bioactive glass content, sintering temperature, and dwelling time gradually improved the mechanical properties of final products which were ascribed to the improved ceramic densification. The composites with the optimal compressive strength were selected to apply in further characterization and cell culture experiments. The selected scaffolds showed excellent bioactivity since a continuous layer of minerals covered the entire surface of composites after immersion in simulated body fluid solution for two weeks. Moreover, the cell culture studies demonstrated that the composite scaffolds could well support the attachment and proliferation of MG-63 osteoblast-like cells. This investigation clearly concluded that the appropriate incorporation of 45S5 bioactive glass into the β-tricalcium phosphate matrix can effectively promote the mechanical behavior, bioactivity, and biocompatibility of the resultant composite scaffolds.

scholarly journals Hydroxyapatite Whisker Reinforced 63s Glass Scaffolds for Bone Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Cijun Shuai ◽  
Yiyuan Cao ◽  
Chengde Gao ◽  
Pei Feng ◽  
Tao Xiao ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Fracture Toughness ◽  
Tissue Engineering ◽  
Compressive Strength ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Selective Laser Sintering ◽  
Composite Scaffolds

Bioactive glass (BG) is widely used for bone tissue engineering. However, poor mechanical properties are the major shortcomings. In the study, hydroxyapatite nanowhisker (HANw) was used as a reinforcement to improve the mechanical properties. 63s glass/HANw scaffolds were successfully fabricated by selective laser sintering (SLS). It was found that the optimal compressive strength and fracture toughness were achieved when 10 wt.% HANw was added. This led to 36% increase in compressive strength and 83% increase in fracture toughness, respectively, compared with pure 63s glass scaffolds. Different reinforcement mechanisms were analyzed based on the microstructure investigation. Whisker bridging and whisker pulling-out were efficient in absorbing crack propagating energy, resulting in the improvement of the mechanical properties. Moreover, bioactivity and biocompatibility of the scaffolds were evaluated in vitro. The results showed that composite scaffolds with 10 wt.% HANw exhibited good apatite-forming ability and cellular affinity.

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