Preparation and characterization of highly porous ceramic-based nanocomposite scaffolds with improved mechanical properties using the liquid phase-assisted sintering method

2018 ◽  
Vol 233 (9) ◽  
pp. 1854-1865
Author(s):  
Mansure Kazemi ◽  
Bahareh Nazari ◽  
Jafar Ai ◽  
Nasrin Lotfibakhshaiesh ◽  
Ali Samadikuchaksaraei ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Culture ◽  
Bioactive Glass ◽  
Tricalcium Phosphate ◽  
Porous Ceramic ◽  
Simulated Body Fluid Solution ◽  
Biological Properties ◽  
Glass Content ◽  
Composite Scaffolds ◽  
Highly Porous

Recent advances in the field of biomaterials have led to the development of ceramic–matrix nanocomposites with enhanced mechanical properties, which is essential for hard tissue scaffolds. In this study, the improvement in mechanical and biological properties of β-tricalcium phosphate reinforced with 45S5 bioactive glass under different sintering conditions was studied. In order to improve the thermal stability and biological responses, β-tricalcium phosphate was doped with 5 mol% strontium ions. Highly porous nanocomposites, with different weight ratios of Sr-tricalcium phosphate/bioactive glass (75/25, 50/50, 25/75), were fabricated through the foam replication method by sintering samples under various thermal conditions (1200–1250 ℃/0–1 h). The effects of bioactive glass content and sintering parameters on microstructure and mechanical behaviors of the nanocomposites were assessed. The obtained results showed that increasing 45S5 bioactive glass content, sintering temperature, and dwelling time gradually improved the mechanical properties of final products which were ascribed to the improved ceramic densification. The composites with the optimal compressive strength were selected to apply in further characterization and cell culture experiments. The selected scaffolds showed excellent bioactivity since a continuous layer of minerals covered the entire surface of composites after immersion in simulated body fluid solution for two weeks. Moreover, the cell culture studies demonstrated that the composite scaffolds could well support the attachment and proliferation of MG-63 osteoblast-like cells. This investigation clearly concluded that the appropriate incorporation of 45S5 bioactive glass into the β-tricalcium phosphate matrix can effectively promote the mechanical behavior, bioactivity, and biocompatibility of the resultant composite scaffolds.

scholarly journals Effect of Melt-Derived Bioactive Glass Particles on the Properties of Chitosan Scaffolds

2019 ◽  
Vol 10 (3) ◽  
pp. 38 ◽  
Author(s):  
Hamasa Faqhiri ◽  
Markus Hannula ◽  
Minna Kellomäki ◽  
Maria Teresa Calejo ◽  
Jonathan Massera
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bioactive Glass ◽  
Pore Size ◽  
Glass Content ◽  
Micro Computed Tomography ◽  
Glass Composite ◽  
Composite Scaffolds ◽  
Bioactive Glasses

This study reports on the processing of three-dimensional (3D) chitosan/bioactive glass composite scaffolds. On the one hand, chitosan, as a natural polymer, has suitable properties for tissue engineering applications but lacks bioactivity. On the other hand, bioactive glasses are known to be bioactive and to promote a higher level of bone formation than any other biomaterial type. However, bioactive glasses are hard, brittle, and cannot be shaped easily. Therefore, in the past years, researchers have focused on the processing of new composites. Difficulties in reaching composite materials made of polymer (synthetic or natural) and bioactive glass include: (i) The high glass density, often resulting in glass segregation, and (ii) the fast bioactive glass reaction when exposed to moisture, leading to changes in the glass reactivity and/or change in the polymeric matrix. Samples were prepared with 5, 15, and 30 wt% of bioactive glass S53P4 (BonAlive ®), as confirmed using thermogravimetric analysis. MicrO–Computed tomography and optical microscopy revealed a flaky structure with porosity over 80%. The pore size decreased when increasing the glass content up to 15 wt%, but increased back when the glass content was 30 wt%. Similarly, the mechanical properties (in compression) of the scaffolds increased for glass content up to 15%, but decreased at higher loading. Ions released from the scaffolds were found to lead to precipitation of a calcium phosphate reactive layer at the scaffold surface. This is a first indication of the potential bioactivity of these materials. Overall, chitosan/bioactive glass composite scaffolds were successfully produced with pore size, machinability, and ability to promote a calcium phosphate layer, showing promise for bone tissue engineering and the mechanical properties can justify their use in non-load bearing applications.

Fabrication and Biological Properties of Bionic Structure Porous Alumina Ceramics with Spherical/Lamellar Pores

2015 ◽  
Vol 815 ◽  
pp. 367-372 ◽  
Author(s):  
Yu Fei Tang ◽  
Qian Miao ◽  
Ying Liu ◽  
Zi Xiang Wu ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Porous Alumina ◽  
Porous Ceramic ◽  
Biological Properties ◽  
Alumina Ceramics ◽  
Pore Connectivity ◽  
Bionic Structure ◽  
Pass Through ◽  
Fabrication Parameters ◽  
Highly Porous

In order to solve the problem that low pore connectivity of the spherical porous ceramic, bionic structure porous alumina ceramics with spherical/lamellar pores were fabricated combining the adding pore-forming agent and two step freeze casting. The effects of fabrication parameters of the samples on morphologies, porosity and pore connectivity are investigated. The mechanical and biological properties of bionic structure porous alumina ceramics are also characterized. Results show that porous alumina ceramics with spherical/lamellar pores are obtained, and the lamellar pores pass through the spherical pores, which prove the pore connectivity reach 86.2 %. The mechanical properties are improved by porosity gradient from the inside (highly porous) to the outside (less porous) of porous alumina. Bionic structure porous alumina ceramics with spherical/lamellar pores have better cell growth and absorbance than those with spherical or lamellar pores only since its high pore connectivity.

scholarly journals A New Generation of Electrospun Fibers Containing Bioactive Glass Particles for Wound Healing

Materials ◽  
2020 ◽  
Vol 13 (24) ◽  
pp. 5651
Author(s):  
Rachele Sergi ◽  
Valeria Cannillo ◽  
Aldo R. Boccaccini ◽  
Liliana Liverani
Keyword(s):  
Mechanical Properties ◽  
Wound Healing ◽  
Bioactive Glass ◽  
Flexible Structures ◽  
Biological Properties ◽  
Cellular Viability ◽  
Bioactive Glasses ◽  
Electrospinning Technique ◽  
Potential Applications ◽  
Biological Performance

Chitosan fibers blended with polyethylene oxide (CHIT_PEO) and crosslinked with genipin were fabricated by electrospinning technique. Subsequently, CHIT_PEO bioactive glass composite electrospun mats were fabricated with the aim to achieve flexible structures with adequate mechanical properties and improved biological performance respect to CHIT_PEO fibers, for potential applications in wound healing. Three different compositions of bioactive glasses (BG) were selected and investigated: 45S5 BG, a Sr and Mg containing bioactive glass (BGMS10) and a Zn-containing bioactive glass (BGMS_2Zn). Particulate BGs (particles size < 20 μm) were separately added to the starting CHIT_PEO solution before electrospinning. The two recently developed bioactive glasses (BGMS10 and BGMS_2Zn) showed very promising biological properties in terms of bioactivity and cellular viability; thus, such compositions were added for the first time to CHIT_PEO solution to fabricate composite electrospun mats. The incorporation of bioactive glass particles and their distribution into CHIT_PEO fibers were assessed by SEM and FTIR analyses. Furthermore, CHIT_PEO composite electrospun mats showed improved mechanical properties in terms of Young’s Modulus compared to neat CHIT_PEO fibers; on the contrary, the values of tensile strain at break (%) were comparable. Biological performance in terms of cellular viability was investigated by means of WST-8 assay and CHIT_PEO composite electrospun mats showed cytocompatibility and the desired cellular viability.

Fabrication of Chitosan Nanofibers Scaffolds with Small Amount Gelatin for Enhanced Cell Viability

2015 ◽  
Vol 749 ◽  
pp. 220-224 ◽  
Author(s):  
Min Sup Kim ◽  
Sang Jun Park ◽  
Bon Kang Gu ◽  
Chun Ho Kim
Keyword(s):  
Mechanical Properties ◽  
Cell Culture ◽  
Cell Viability ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Biological Properties ◽  
Human Dermal Fibroblast ◽  
Initial Contact ◽  
Initial Contact Angle ◽  
Contact Angle Analysis

Chitosan and gelatin has attracted considerable interest owing to its advantageous biological properties such as excellent biocompatibility, biodegradation, and non-toxic properties. In this paper, we investigated the potential of chitosan/gelatin (Chi-Gel) nanofibers mat with enhanced cell viability for use as cell culture scaffolds. The surface morphology, mechanical properties, and initial contact angle analysis of Chi-Gel nanofibers mat were evaluated. The proliferation of human dermal fibroblast cell (HDFs) on Chi-Gel nanofibers mat was found to be approximately 20% higher than the pure chitosan nanofibers mat after 7 days of culture. These results suggest that the Chi-Gel nanofibers mat has great potential for use tissue engineering applications.

scholarly journals Manufacturing and Characterization of Highly Porous Bioactive Glass Composite Scaffolds Using Unidirectional Freeze Casting

2017 ◽  
Vol 19 (10) ◽  
pp. 1700129 ◽  
Author(s):  
Laura M. Henning ◽  
Sara Zavareh ◽  
Paul H. Kamm ◽  
Miriam Höner ◽  
Horst Fischer ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Freeze Casting ◽  
Glass Composite ◽  
Composite Scaffolds ◽  
Highly Porous

scholarly journals Novel Bioactive Glass-Modified Hybrid Composite Resin: Mechanical Properties, Biocompatibility, and Antibacterial and Remineralizing Activity

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiao Han ◽  
Yan Chen ◽  
Qian Jiang ◽  
Xin Liu ◽  
Yaming Chen
Keyword(s):  
Mechanical Properties ◽  
Bioactive Glass ◽  
Hybrid Composite ◽  
Composite Resin ◽  
Ph Value ◽  
Biological Properties ◽  
Composite Resins ◽  
Mass Fractions ◽  
Good Biocompatibility ◽  
Insight Into

Secondary caries seriously limits the lifetime of composite resin. However, integrating all desirable properties (i.e., mechanical, antibacterial, bioactivity, and biocompatibility) into one composite resin is still challenging. Herein, a novel bioactive glass (BAG)-modified hybrid composite resin has been successfully developed to simultaneously achieve excellent mechanical properties, good biocompatibility, and antibacterial and remineralizing capabilities. When the mass fractions of BAG particles were added from 8 to 23 wt %, the original mechanical properties of the composite resin, including flexural strength and compressive strength, were not obviously affected without compromising the degree of conversion. Although the BAG incorporation of mass fractions of 16 wt % to 23 wt % in composite resins reduced cell viability, the viability could be recovered to normal by adjusting the pH value. Moreover, the BAG-modified composite resins that were obtained showed good antibacterial effects against Streptococcus mutans and enhanced remineralizing activity on demineralized dentin surfaces with increasing incorporation of BAG particles. The possible mechanisms for antibacterial and remineralizing activity might be closely related to the release of bioactive ions (Ca2+, Si4+), suggesting that its antibacterial and biological properties can be controlled by modulating the amounts of bioactive ions. The capability to balance the mechanical properties, cytotoxicity, antibacterial activity, and bioactivity makes the BAG-modified composite resin a promising prospect for clinical application. Our findings provide insight into better design and intelligent fabrication of bioactive composite resins.

Improved Osteogenesis by Noggin siRNA Collagen-Bioactive Glass Composites

2021 ◽  
Vol 11 (8) ◽  
pp. 1600-1605
Author(s):  
Yanling Chen ◽  
Fan Xue ◽  
Liangjiao Chen ◽  
Zhengmao Li ◽  
Zedong Lan
Keyword(s):  
Bioactive Glass ◽  
Composite Scaffold ◽  
Bone Defects ◽  
Biological Properties ◽  
Negative Control ◽  
Regeneration Ability ◽  
Quantitative Real Time Pcr ◽  
Composite Scaffolds ◽  
Alizarin Red ◽  
Glass Composites

Collagen-bioactive glass (COL–BG) composites have significant bone regeneration ability due to the considerable osteogenic performance and desirable biological properties of collagen. In the current research, the influence of COL–BG composite scaffolds loaded with noggin siRNA on osteogenesis in MC3T3 cells have been comprehensively studied. We prepared three types of COL–BG composites scaffolds, namely, unloaded, loaded with noggin siRNA, and loaded with negative control siRNA. The effect of the aqueous extracts achieved from these scaffolds on MC3T3 cell proliferation was monitored using a CCK8 test, whilst the effect on osteogenesis was assessed with ALP (alkaline phosphatase) activity assays, quantitative real-time PCR, and Alizarin Red staining (ARS). After cells were cultured for three and five days, proliferation in the presence of the extracts of composite scaffolds was remarkably greater than in the no-scaffold blank (P < 0.05). After 14 days of cultivation, MC3T3 cells exposed to COL–BG composites scaffold loaded with noggin siRNA exhibited greater ALP performance (P < 0.05) as well as higher mRNA expression (P < 0.01) of Runx2, BSP, and ALP genes in comparison with two other composite scaffolds, whereas ARS exhibited a larger number of mineralized nodules. Overall, these outcomes illustrate that COL–BG composite scaffold loaded with noggin siRNA is effective in enhancing osteogenesis. Thus, we suggest that this material has a promising potential for bone tissue engineering and is able to locally improve bone formation in bone defects.

scholarly journals A Comparative Evaluation of the Mechanical Properties of Two Calcium Phosphate/Collagen Composite Materials and Their Osteogenic Effects on Adipose-Derived Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Qing Li ◽  
Tong Wang ◽  
Gui-feng Zhang ◽  
Xin Yu ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Composite Materials ◽  
Calcium Phosphate ◽  
Tricalcium Phosphate ◽  
Comparative Evaluation ◽  
Bone Defects ◽  
Adipose Derived Stem Cells ◽  
Composite Scaffolds

Adipose-derived stem cells (ADSCs) are ideal seed cells for use in bone tissue engineering and they have many advantages over other stem cells. In this study, two kinds of calcium phosphate/collagen composite scaffolds were prepared and their effects on the proliferation and osteogenic differentiation of ADSCs were investigated. The hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) composite scaffolds (HTPSs), which have an additionalβ-tricalcium phosphate, resulted in better proliferation of ADSCs and showed osteogenesis-promoting effects. Therefore, such composite scaffolds, in combination with ADSCs or on their own, would be promising for use in bone regeneration and potential clinical therapy for bone defects.

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