scholarly journals The Association of IgE Levels with ADAM33 Genetic Polymorphisms among Asthmatic Patients

2021 ◽  
Vol 11 (5) ◽  
pp. 329
Author(s):  
Malek Zihlif ◽  
Amer Imraish ◽  
Baeth Al-Rawashdeh ◽  
Aya Qteish ◽  
Raihan Husami ◽  
...  
Keyword(s):  
Genetic Polymorphisms ◽  
Immunoglobulin E ◽  
Allergic Diseases ◽  
Total Serum ◽  
P Value ◽  
Asthmatic Patients ◽  
Minor Alleles ◽  
Adults And Children ◽  
Polymerase Chain ◽  
Control Study

Total serum immunoglobulin E (IgE) is elevated in multiple allergic diseases and is considered a good predictor of atopy. Several studies have been performed on the association of IgE levels with the polymorphism of the ADAM33 gene in asthmatic patients. The aim of this study was to determine whether there is an association between IgE levels and the genetic polymorphisms of the ADAM33 gene (T1, T2, T + 1, V4, S1, S2, and Q-1) in both healthy and asthmatic patients among Jordanians. The clinical data were collected for this case–control study from 267 asthmatic patients and 225 control subjects. Seven genetic polymorphisms (T1, T2, T + 1, V4, S1, S2, and Q-1) of the gene ADAM33 were analyzed using the polymerase chain reaction/restriction fragment length polymorphism method. The minor alleles (G) of T1, (A) of T2, T + 1, and (G) of V4 polymorphisms were associated with a significant increase in total serum IgE levels in adults but not children. The V4 genetic polymorphism, however, showed a significant association with IgE levels in both adults and children. The S1 polymorphism was significantly associated with the codominant module only in the adults. The S2 polymorphism showed a significant association (p-value < 0.05) in both codominant and recessive models. However, in the dominant model for both pediatric control and asthmatic patients, the association between the IgE and S2 polymorphism was insignificant (p-value = 0.7271 and 0.5259, respectively). This study found a statistically significant association between multiple ADAM33 genetic polymorphisms and IgE levels. Such findings add to the growing evidence that the ADAM33 gene has a major impact on IgE levels among asthmatic patients of Jordanian origin.

scholarly journals Association of IL13R Alpha 1 + 1398A/G Polymorphism in a North Indian Population with Asthma: A Case-Control Study

2015 ◽  
Vol 6 (2) ◽  
pp. ar.2015.6.0126
Author(s):  
Shweta Sinha ◽  
Jagtar Singh ◽  
Surinder Kumar Jindal ◽  
Niti Birbian
Keyword(s):  
Indian Population ◽  
Immunoglobulin E ◽  
Airway Hyperreactivity ◽  
Total Serum ◽  
North Indian Population ◽  
Increased Risk ◽  
Asthma Patients ◽  
Polymerase Chain ◽  
Control Study

Background Interleukin 13 (IL13) is directly involved in the secretion of total serum immunoglobulin E (IgE), which plays a major role in the asthma pathogenesis. Objective One of the polymorphic receptor of IL13 is IL13Rα1, which after binding to IL13, initiates signal transduction that results in mucin secretion, airway hyperreactivity, fibrosis, and chitinase up-regulation, which increases asthma risk. Methods In the present study, the role of IL13Rα1 + 1398A/G gene polymorphisms in asthma was detected with a total of 964 individuals, including 483 healthy controls and 481 asthma patients from a North Indian population using polymerase chain reaction-restriction fragment length polymorphism method. Results Statistical analysis revealed that the mutant allele (G) is predominant in asthma patients (42.7%) than the controls (38.2%), which shows an increased risk toward asthma with odds ratio = 1.21, 95% confidence interval (1.00 −1.45), χ2 = 4.10 and p = 0.043. Furthermore, the phenotypic characteristics also reveal a significant association with the disease (p < 0.05). Conclusions This is the first study conducted in India and + 1398A/G polymorphism in noncoding region of IL13Rα1 confer risk toward asthma in the studied population.

Genetic Polymorphisms in the Homocysteine Metabolism Pathway and Thrombosis in Thai Children: A Case Control Study.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 5493-5493
Author(s):  
Nongnuch Sirachainan ◽  
Pornsri Tapanapruksakul ◽  
Werasak Sasanakul ◽  
Anannit Visudtibhan ◽  
Pimlak Charoenkwan ◽  
...  
Keyword(s):  
Genetic Polymorphisms ◽  
Methylenetetrahydrofolate Reductase ◽  
Control Group ◽  
P Value ◽  
Thai Population ◽  
Increase Risk ◽  
Genes Encoding ◽  
Adults And Children ◽  
Risk For Thrombosis ◽  
Control Study

Abstract Hyperhomocysteinemia is a known risk factor for thrombosis in both adults and children. Mutations of genes encoding the enzymes in homocysteine metabolism are predicted to cause elevation of homocysteine and increase the risk of thrombosis. Therefore, this study objectives are to determine the prevalence of methylenetetrahydrofolate reductase (MTHFR) 677C->T, 1298A->C; methionine synthase (MS) 2756A->G, and cystathione β-synthase (CBS) 884ins68 in Thai ethnic patients, aged ≤18 years, and their relationship to thrombosis. The population consisted of 91 patients, from 2 medical institutes in Thailand, first diagnosed with thrombosis. The control group was 169 healthy, Thai children matched for age (mean ± SD in cases/controls = 9.4 ± 5.7 / 9.3 ± 5.7 years, p = 0.9) and sex (male/female in cases/controls = 57.1%/42.9%/53.8%/46.2%, p = 0.7). Genotyping was performed using standard PCR/restriction enzyme assays. The prevalence and allele frequency of the genes are shown in Table 1. The polymorphisms of individual genes and the combinations between the genotype did not affect plasma homocysteine level. The details are shown in Table 2. Conclusion, the prevalence of MTHFR 677C->T, 1298A->C, MS 2756A->G, and CBS 884ins68 in Thai population was lower than Caucasian population; and did not cause the increase risk for thrombosis in Thai children. Table 1. Frequency of genetic polymorphisms between patients and controls. MTHFR 677A CC (%) CT (%) TT (%) T frequency (%) Controls 71.8 27 1.2 14.7 Patients 73.5 25.3 1.2 - p-value - 0.91 0.96 - MTHFR 1298 AA (%) AC (%) CC (%) C frequency (%) Controls 50.0 42.1 7.9 24.4 Patients 54.3 42.0 3.7 - p-value - 0.76 0.26 - MS 2756 AA (%) AG (%) GG (%) G frequency (%) Controls 78.9 21.1 0 10.5 Patients 75.9 24.1 0 - p-value - 0.63 - - CBS 884ins68 NN (%) NI (%) II (%) G frequency (%) Controls 98.7 0.6 0.6 1 Patients 98.7 1.3 0 - p-value - 0.55 - - Table 2. Homocysteine value and genetic polymorphisms. Genetic polymorphism (number) Plasma Hcy (μmol/L) p value MTHFR 677 CC (n=105) 7.4 ± 2.9 0.11 CT (n=29) 8.3 ± 2.7 MTHFR 1298 AA (n=64) 7.4 ± 2.4 > 0.05 AC (n=59) 7.5 ± 2.8 > 0.05 CC (n=12) 8.7 ± 5 MS 2756 AA (n=110) 7.8 ± 3 AG (n=25) 6.7 ± 1.9 0.055 CBS 884 ins 68 DD (n=137) 7.5 ± 2.8 ID (n=2) 10.3 ± 8.7 0.72 MTHFR 677 / MTHFR 1298 CT/AC (n=16) 8.7 ± 2.5 0.07 MTHFR 1298 / MS 2765 AC/AG (n=6)6.8 ± 1.6 6.8 ± 1.6 0.38 MTHFR 1298 / CBS884ins68 AC/IN (n=9) 6.8 ± 2.1 0.34

scholarly journals The Study of a Possible Correlation between Serum Levels of Interleukin 17 and Clinical Severity in Patients with Allergic Rhinitis

2017 ◽  
Vol 8 (3) ◽  
pp. ar.2017.8.0207
Author(s):  
Mai Aly Gharib Aly ◽  
Mohamed Tawfik El Tabbakh ◽  
Waheed Fawzy Heissam ◽  
Said Hamed Abbadi
Keyword(s):  
Allergic Rhinitis ◽  
Immunoglobulin E ◽  
Skin Testing ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Clinical Severity ◽  
Total Serum ◽  
Interleukin 17 ◽  
Serum Ige ◽  
Cluster Immunotherapy

Introduction Allergic rhinitis (AR) is one of the most common allergic diseases, which affects ~20% of the world's population. T-helper (Th) type 2 cells produce interleukin (IL) 4 and IL-13, and mediate allergic responses, and these cytokines have been extensively studied as key players in the atopic airway diseases. However, the involvement of Th17 cells and IL-17 in AR has not been clearly examined. Aim To reevaluate AR clinical severity with serum IL-17, whether IL-17 affects the disease alone or in contribution with the atopic predisposition. Patients and Methods During an 18-month period, 39 individuals were divided into three groups: A, (13 control), B (13 with mild-to-moderate AR), and C (13 with severe AR). Both group B and group C patients (26) were subjected to clinical examination and allergy skin testing, and to measurement of both total serum immunoglobulin E (IgE) and IL-17 levels. Eleven patients with AR then were exposed to 6 months of cluster immunotherapy, whereas the rest of the patients were not exposed. Results Revealed a significant elevation of serum IL-17 levels with an associated increase in serum IgE in the patients with AR compared with controls and revealed that the serum levels of both total serum IgE and IL-17 decreased significantly after cluster immunotherapy. Conclusion These preliminary results added new data about the use of injective immunotherapy as well as reported on the use of sublingual immunotherapy.

Dose-Dependent Effect of Cotinine-Verified Tobacco Smoking on Serum Immunoglobulin E Levels in Korean Adult Males

2017 ◽  
Vol 21 (6) ◽  
pp. 813-817 ◽  
Author(s):  
Jae-June Dong ◽  
Jay J Shen ◽  
Yong-Jae Lee
Keyword(s):  
Tobacco Smoking ◽  
Immunoglobulin E ◽  
Allergic Diseases ◽  
Serum Immunoglobulin ◽  
Total Serum ◽  
Adult Males ◽  
Serum Ige ◽  
Korean Adult ◽  
Dose Dependent ◽  
Dose Dependent Effect

Abstract Background Smoking is one of the risk factors to exacerbate allergic diseases, and it may affect serum immunoglobulin E (IgE) levels. However, few studies have relied on an objective biomarker to examine the effect of tobacco smoking on serum IgE levels. Method A nationwide cross-sectional study was conducted to examine the relationship between urinary cotinine (Ucot) concentrations and IgE levels in 973 males using data from the 2010 Korean National Health and Nutrition Examination Survey (KNHANES). Ucot was classified into four groups based on concentration (ng/mL) as follows: nonsmoker group (Ucot &lt;50 ng/mL) and three tertile groups in smokers (T1 [Ucot: 50.00–921.28 ng/mL]; T2 [Ucot: 921.29–1869.36 ng/mL]; and T3 [Ucot ≥1869.37 ng/mL]). The dose-response relationships between Ucot concentrations and total serum IgE level were estimated using analysis of covariance (ANCOVA) and multiple linear regression analysis after adjusting for confounding variables. Results We found a significant and positive dose-related effect of cigarette smoking as measured by Ucot concentrations on the total serum IgE level. The multivariate adjusted means of total serum IgE levels (SE) were 321.0 (36.3), 404.4 (102.7), 499.2 (79.2), and 534.7 (82.7) IU/mL, after adjusting for age, body mass index, alcohol ingestion, physical exercise, job, and household income. The regression coefficient β for total serum IgE was β = 68.6 with increasing level of Ucot group after adjusting for the same covariables (p = .009). Conclusion These findings suggest that the amount of smoking may have a dose-dependent effect on total serum IgE levels. Implication Smoking is one of the risk factors to exacerbate allergic diseases, and it may affect serum immunoglobulin E (IgE) levels, which is closely related to type 1 mediated allergic diseases. However, few studies have relied on an objective biomarker to examine the effect of tobacco smoking on serum IgE levels. We found that tobacco exposure, as measured by Ucot concentrations, increased the serum IgE levels in a dose-response manner in a representative sample of Korean adult males.

scholarly journals Genetic polymorphisms of inflammasome genes associated with pediatric acute lymphoblastic leukemia and clinical prognosis in the Brazilian Amazon

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fabíola Silva Alves ◽  
Lilyane Amorim Xabregas ◽  
Marlon Wendell Athaydes Kerr ◽  
Gláucia Lima Souza ◽  
Daniele Sá Pereira ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Genetic Polymorphisms ◽  
Childhood Leukemia ◽  
Fragment Length Polymorphism ◽  
Lymphoblastic Leukemia ◽  
Clinical Prognosis ◽  
Real Time Quantitative Pcr ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Control Study

AbstractThe immune system plays an important role in the control of cancer development. To investigate the possible association of inflammasome genes to childhood leukemia we performed a case-control study with 158 patients with acute lymphoblastic leukemia and 192 healthy individuals. The IL1B and IL18 genetic polymorphisms were genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and NLRP1, NLRP3 and P2RX7 were genotyped using Real Time quantitative PCR (qPCR). The IL1B C/T rs19644 genotype was associated with the risk of developing ALL (C/C vs. C/T + T/T OR: 2.48 [95% CI: 1.26–4.88, p = 0.006]; C/C vs C/T OR: 2.74 [95% CI: 1.37–5.51, p = 0.003]) and the NLRP1 A/T rs12150220 (OR: 0.37 [95% CI: 0.16–0.87, p = 0.023]) was associated with protection against infectious comorbidities. It was not found association between NLRP3 and P2RX7 polymorphisms and acute lymphoblastic leukemia in our study. Our results suggest that the inflammasome single-variant polymorphisms (SNVs) may play a role in the development and prognostic of childhood leukemia. However, this finds requires further study within a larger population in order to prove it.

Genetic polymorphisms of Cathepsin B are associated with gastric cancer risk and prognosis in a Chinese population

2021 ◽  
pp. 1-10
Author(s):  
Xiang Ma ◽  
Younan Wang ◽  
Hao Fan ◽  
Chuming Zhu ◽  
Wangwang Chen ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Genetic Polymorphisms ◽  
Cathepsin B ◽  
Case Control Study ◽  
Nucleotide Polymorphisms ◽  
Sequencing Technology ◽  
Single Nucleotide ◽  
Polymerase Chain ◽  
Control Study

BACKGROUND: Genetic polymorphisms are believed to represent a key aspect of predisposition to gastric cancer (GC). Therefore, considering the important role of Cathepsin B (CTSB) in promoting cancer onset and development, it could be very worthful to explore the function of CTSB-related genetic polymorphisms in GC. OBJECTIVE: In this study, we investigated the correlation of CTSB-related polymorphisms (rs9009A>T, rs6731T>C, rs1293303G>C, rs1874547C>T, rs3779659C>T, rs17814426C>T and rs148669985C>T) with GC risk and prognosis in a case-control study of 994 cases and 1000 controls. METHODS: All tag single nucleotide polymorphisms (SNPs) were genotyped by polymerase chain reaction-ligase detection reaction (PCR-LDR) sequencing technology. RESULTS: The results indicated rs9009, rs6731 and rs17814426 correlated with decreased risks of GC (HR = 0.97, p< 0.001; HR = 0.86, P= 0.019; HR = 0.85, P= 0.017; respectively). Stratification analysis further showed rs17814426 variant genotypes correlated with earlier T stage (p= 0.044). In addition, GC patients carrying the C allele of rs6371 had better overall prognosis (HR = 0.62, 95%CI = 0.44–0.88). CONCLUSION: Our results firstly suggested the importance of CTSB-related polymorphisms on GC which could predict GC risk and prognosis.

scholarly journals Evaluation of some trace elements in sera of asthma patients: a case control study.

Folia Medica ◽  
2021 ◽  
Vol 63 (5) ◽  
pp. 797-804
Author(s):  
Falah S. Al-Fartusie ◽  
Manal J. Abood ◽  
Hassanain K. Al-Bairmani ◽  
Ahmed S. Mohammed
Keyword(s):  
Trace Elements ◽  
Allergic Diseases ◽  
Control Group ◽  
Asthmatic Patients ◽  
Atomic Absorption Technique ◽  
Asthma Patients ◽  
Low Levels ◽  
And Control ◽  
Control Study ◽  
Absorption Technique

Introduction: Asthma is a chronic in&#64258;ammatory disease of the airways characterized by recurrent respiratory symptoms of dyspnea, wheezing, chest tightness, and cough. Aim: This study aims to investigate the concentrations of Cu, Zn, Mg, Mn, Fe, Cr, Ni, and Al in the serum of asthmatic patients. Materials and methods: An atomic absorption technique was used to determine the levels of trace elements. The study included sixty asthmatic patients and ninety healthy individuals as control group their ages ranging from 20 to 45 years. Results: We found a significant increase of the levels of copper, iron, and aluminum by 20%, 54%, and 47% (p<0.01), respectively, in the asthmatic patients as compared with the controls. On the other hand, in comparison with the controls, the levels of zinc, magnesium, manganese, and nickel were found to be significantly decreased (p<0.01) by 24%, 16%, 53%, and 81%, respectively, in the asthmatic patient group. Moreover, chromium level showed non-significant differences (p>0.05) between patients and control group. Conclusions: The increased level of Cu and Fe may reflect their potential role in the pathogenesis of asthma. Furthermore, the serum iron levels tend to be increased as a result of the inflammatory process that occurs in asthma. Low levels of Zn, Mg, and Mn may contribute to allergic diseases due to their role in the synthesis of certain antioxidants or to their effect on the immune system.

scholarly journals TREM1 rs2234237 (Thr25Ser) Polymorphism in Patients with Cutaneous Leishmaniasis Caused by Leishmania guyanensis: A Case-Control Study in the State of Amazonas, Brazil

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 498
Author(s):  
José do Espírito Santo Júnior ◽  
Tirza Gabrielle Ramos de Mesquita ◽  
Luan Diego Oliveira da Silva ◽  
Felipe Jules de Araújo ◽  
Josué Lacerda de Souza ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Fragment Length Polymorphism ◽  
P Value ◽  
Tnf Α ◽  
Plasma Cytokines ◽  
Polymerase Chain ◽  
Leishmania Guyanensis ◽  
Control Study ◽  
Leishmania Parasites ◽  
Modest Effect

Background: Leishmaniasis is an infectious disease caused by Leishmania parasites. A Th1 immune response is necessary in the acute phase to control the pathogen. The triggering receptor expressed on myeloid cells (TREM)-1 is a potent amplifier of inflammation. Our aim is to identify whether the TREM1 variant rs2234237 A/T (Thr25Ser) is associated with the disease development of cutaneous leishmaniasis (CL) in Leishmania guyanensis-infected individuals. The effects of the rs2234237 genotypes on plasma cytokines IL-1β, IL-6, IL-8, IL-10, MCP-1 and TNF-α are also investigated. Methods: 838 patients with CL and 818 healthy controls (HCs) living in the same endemic areas were genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism. Plasma cytokines were assayed in 400 patients with CL and 400 HCs using the BioPlex assay. Results: The genotypes’ and alleles’ frequencies were similar in both patients with CL (AA = 618, 74%; AT = 202, 24%; TT = 18, 2%) and in HCs (AA = 580, 71%; AT = 220, 27%; TT = 18, 2%). Rs2234237 showed a modest effect on plasma IL-10 that disappeared when correction of the p-value was applied. Plasma IL-10 by rs2234237 genotypes were (mean ± SEM; AA = 2.91 pg/mL ± 0.14; AT = 2.35 pg/mL ± 0.12; TT = 3.14 pg/mL ± 0.56; p = 0.05). Conclusion: The TREM1 rs2234237 (Thr25Ser) seems to have no influence on the susceptibility or resistance to L. guyanensis infections.

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