scholarly journals Artificial, Triple-Layered, Nanomembranous Wound Patch for Potential Diabetic Foot Ulcer Intervention

Materials ◽  
2018 ◽  
Vol 11 (11) ◽  
pp. 2128 ◽  
Author(s):  
Mostafa Mabrouk ◽  
Pradeep Kumar ◽  
Yahya E. Choonara ◽  
Lisa C. du Toit ◽  
Viness Pillay
Keyword(s):  
Mechanical Properties ◽  
Drug Release ◽  
Diabetic Foot ◽  
Drug Loading ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Middle Layer ◽  
Patch Quality ◽  
Moisture Uptake

The present work aims to electrospin a triple layered wound patch for potential treatment of diabetic foot ulcers (DFU). The patch consisted of poly(acrylic acid) (PAA) as the skin contacting layer, polyvinyl pyrrolidone (PVP) as the middle layer, and polycaprolactone (PCL) as the outermost layer, wherein the PVP layer was loaded in situ with an antibiotic (ciprofloxacin, CFX). Morphology and mechanical properties were investigated using SEM and texture analysis. Patch quality was studied with regards to wettability, adherence, water resistance, and moisture uptake of individual layers. SEM results confirmed the fibrous and membranous nature of layers with a nano-to-micro size range. Mechanical properties of the composite patch demonstrated a tensile strength of 12.8 ± 0.5 MPa, deformation energy of 54.35 ± 0.1 J/m3, and resilience of 17.8 ± 0.7%, which were superior compared to individual layers. Patch quality tests revealed that the PCL layer showed very low wettability, adherence, and moisture uptake compared to the PVP and PAA layers. In vitro drug release data revealed an increase in cumulative drug release with higher drug loading. The results above confirm the potential of a triple layered, tripolymeric, wound patch for DFU intervention.

Valsartan solid lipid nanoparticles integrated hydrogel: A challenging repurposed use in the treatment of diabetic foot ulcer, in-vitro/in-vivo experimental study

2021 ◽  
Vol 592 ◽  
pp. 120091
Author(s):  
Noha S. El-Salamouni ◽  
Mennatallah A. Gowayed ◽  
Nevine L. Seiffein ◽  
Rehab A. Abdel- Moneim ◽  
Maher A. Kamel ◽  
...  
Keyword(s):  
Experimental Study ◽  
Diabetic Foot ◽  
Solid Lipid Nanoparticles ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Lipid Nanoparticles ◽  
Solid Lipid

scholarly journals Not Only Antimicrobial: Metronidazole Mitigates the Virulence of Proteus mirabilis Isolated from Macerated Diabetic Foot Ulcer

2021 ◽  
Vol 11 (15) ◽  
pp. 6847
Author(s):  
Ahdab N. Khayyat ◽  
Hisham A. Abbas ◽  
Mamdouh F. A. Mohamed ◽  
Hani Z. Asfour ◽  
Maan T. Khayat ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Proteus Mirabilis ◽  
Diabetic Foot ◽  
Biofilm Formation ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Foot Ulcers ◽  
Resistant Bacteria ◽  
Diabetic Foot Ulcers

Diabetic foot ulcers are recognized to be a severe complication of diabetes, increasing the risk of amputation and death. The bacterial infection of Diabetic foot ulcers with virulent and resistant bacteria as Proteus mirabilis greatly worsens the wound and may not be treated with conventional therapeutics. Developing new approaches to target bacterial virulence can be helpful to conquer such infections. In the current work, we evaluated the anti-virulence activities of the widely used antibacterial metronidazole. The minimum inhibitory concentrations (MIC) and minimum biofilm eradication concentrations (MEBC) were determined for selected antibiotics which P. mirabilis was resistant to them in the presence and absence of metronidazole in sub-MIC. The effect of metronidazole in sub-MIC on P. mirabilis virulence factors as production of exoenzymes, motilities, adhesion and biofilm formation, were evaluated. Furthermore, molecular docking of metronidazole into P. mirabilis adhesion and essential quorum sensing (QS) proteins, was performed. The results revealed a significant ability of metronidazole to in-vitro inhibit P. mirabilis virulence factors and antagonize its essential proteins. Moreover, metronidazole markedly decreased the MICs and MBECs of tested antibiotics. Conclusively, metronidazole in sub-MIC is a plausible anti-virulence and anti-QS agent that can be combined to other antibiotics as anti-virulence adjuvant to defeat aggressive infections.

scholarly journals Synthesis And Evaluation Of Wound Healing Properties of HyDrO-DiAb Hydrogel Loaded With Green-Synthetized AGNPS: In Vitro And In Ex Vivo Studies

2021 ◽  
Author(s):  
Mariarosa Ruffo ◽  
Ortensia Ilaria Parisi ◽  
Marco Dattilo ◽  
Francesco Patitucci ◽  
Rocco Malivindi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Diabetic Foot ◽  
Ex Vivo ◽  
Foot Ulcer ◽  
Vascular Diseases ◽  
Diabetic Foot Ulcer ◽  
Wound Dressings ◽  
Diabetic Patients ◽  
Wound Healing Activity

Abstract In diabetic patients, the presence of neuropathy, peripheral vascular diseases and ischemia, leads to the formation of foot ulcerations with a higher risk of infection because the normal response to bacterial infection is missing. In the aim to control and treat Diabetic Foot Ulcerations (DFUs), wound dressings able to absorb exudate, to prevent infections and to promote wound healing, are needed. For this reason, the aim of the present research was to synthetize a biocompatible hydrogel composed by Carboxymethylcellulose (HyDrO-DiAb) loaded with Silver nanoparticles (AgNPs) for the treatment of diabetic foot ulcer. In this study, AgNPs were obtained by a green synthesis and, then, were dissolved in CMC hydrogel that, after freeze drying process become a flexible and porous structure. The in vitro and in ex-vivo wound healing activity of the obtained HyDrO-DiAb hydrogel was evaluated.

scholarly journals Electrospun cellulose acetate/gelatin nanofibrous wound dressing containing berberine for diabetic foot ulcer healing: in vitro and in vivo studies

2019 ◽  
Author(s):  
Hadi Samadian ◽  
Arian Ehterami ◽  
Saeed Farzamfar ◽  
Ahmad Vaez ◽  
Hossein Khastar ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Diabetic Foot ◽  
Wound Dressing ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Biological Properties ◽  
In Vivo Studies ◽  
Vapor Permeability

AbstractFunctional dressing with tailored physicochemical and biological properties is vital for diabetic foot ulcer (DFU) treatment. Our main objective in the current study was to fabricate Cellulose Acetate/Gelatin (CA/Gel) electrospun nanofibrous mat loaded with berberine (Beri) as the DFU dressing. The results demonstrated that the diameter of the nanofibers was around 502 nm, the tensile strength, contact angle, porosity, water vapor permeability, and water uptake ratio of CA/Gel nanofibers were around 2.83 MPa, 58.07, 78.17 %, 11.23 mg/cm2 hr, and 12.78 respectively, while these values for CA/Gel/Beri nanofibers were 2.69 ± 0.05 MPa, 56.93 ± 1, 76.17 ± 0.76 %, 10.17 ± 0.21 mg/cm2 hr, 14.37 ± 0.42 respectively. The bacterial evaluations demonstrated that the dressings are an excellent barrier against bacterial penetration with potent antibacterial activity. The animal studies depicted that the collagen density and angiogenesis score in the CA/Gel/Beri treated group were 88.8±6.7 % and 19.8±3.8, respectively. These findings implied that the incorporation of berberine did not compromise the physical properties of dressing, while improving the biological activates. In conclusion, our findings implied that the prepared mat is a proper wound dressing for DFU management and treatment.

scholarly journals The Anti-Inflammatory and Antibacterial Action of Nanocrystalline Silver and Manuka Honey on the Molecular Alternation of Diabetic Foot Ulcer: A Comprehensive Literature Review

2015 ◽  
Vol 2015 ◽  
pp. 1-19 ◽  
Author(s):  
Ka-Kit Tsang ◽  
Enid Wai-Yung Kwong ◽  
Kevin Y. Woo ◽  
Tony Shing-Shun To ◽  
Joanne Wai-Yee Chung ◽  
...  
Keyword(s):  
Diabetic Foot ◽  
Animal Studies ◽  
Chronic Wounds ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
In Vivo Studies ◽  
Resistant Bacteria ◽  
Manuka Honey ◽  
Anti Inflammatory

Honey and silver have been used since ancient times for treating wounds. Their widespread clinical application has attracted attention in light of the increasing prevalence of antibiotic-resistant bacteria. While there have been a number of studies exploring the anti-inflammatory and antibacterial effects of manuka honey and nanocrystalline silver, their advantages and limitations with regard to the treatment of chronic wounds remain a subject of debate. The aim of this paper is to examine the evidence on the use of nanocrystalline silver and manuka honey for treating diabetic foot ulcers through a critical and comprehensive review of in vitro studies, animal studies, and in vivo studies. The findings from the in vitro and animal studies suggest that both agents have effective antibacterial actions. Their anti-inflammatory action and related impact on wound healing are unclear. Besides, there is no evidence to suggest that any topical agent is more effective for use in treating diabetic foot ulcer. Overall, high-quality, clinical human studies supported by findings from the molecular science on the use of manuka honey or nanocrystalline silver are lacking. There is a need for rigorously designed human clinical studies on the subject to fill this knowledge gap and guide clinical practice.

Formulation and Evaluation of Transdermal Patches of Benidipine Hydrochloride

2021 ◽  
pp. 207-212
Author(s):  
Kalpak Gajbhiye ◽  
Nawaz Hakam ◽  
Gauri Rathod ◽  
Mukund Tawar
Keyword(s):  
Mechanical Properties ◽  
Drug Release ◽  
Physical And Mechanical Properties ◽  
Release Profile ◽  
Moisture Uptake ◽  
In Vitro Drug Release ◽  
Percent Elongation ◽  
Transdermal Patches ◽  
Release Property

The present research was designed to evaluate matrix type Benidipine Hydrochloride transdermal patches. So, the present work is an attempt to study effect of variation in Methocel E15LV concentration and study effect of hydrophilic and hydrophobic Methocel E15LV and Eudragit RL100 combination on release profile of Benidipine Hydrochloride. All prepared formulations were evaluated for physical and mechanical properties like thickness, moisture uptake, percent flatness, tensile strength, and percent elongation. An attempt was made to get a patch with suitable drug release property as well as physical and mechanical properties. A formulation containing Methocel E15LV 2% has shown good physical, mechanical and in-vitro drug release properties. It also showed good diffusion of Benidipine Hydrochloride across goat skin.

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