Bionanotechnology: Silver Nanoparticles Supported on Bovine Bone Powder Used as Bactericide

Bionanotechnology is a relatively new term that implies the use of some biological material or organisms in order to prepare nanosystems or nanoparticles. This work presents the preparation and bactericide application of a sustainable nanometric system (silver nanoparticles) using a waste biological support (bovine bone powder). This system was prepared by the method of metallic salt reduction, using NaBH4 as reducing agent and AgNO3 as metallic salt. Two silver contents were analyzed, 1% and 5% weight. The latter was found to be more efficient than the former. Transmission electronic microscopy shows an average size of 10.5 ± 3.3 nm and quasi-sphere morphology. The antimicrobial assay shows that a 5% weight content of silver had a bactericide effect for Escherichia coli at 46.8 min of exposure. The minimum inhibitory concentration (MIC) of silver nanoparticles supported on bovine bone powder for Escherichia coli was 7.5 µg/mL. The biocomposite exhibits a specific antibacterial kinetics constant (k) of 0.1128 min−1 and decimal reduction time (DRT) of 20.39 min for Escherichia coli. Thus, it was concluded that a biocomposite was prepared with a biodegradable, waste, and low-cost support, under mild conditions (room temperature and atmospheric pressure) and using water as solvent.

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Statistically Optimized Production of Saccharides Stabilized Silver Nanoparticles Using Liquid–Plasma Reduction Approach for Antibacterial Treatment of Water

Materials ◽

10.3390/ma14195841 ◽

2021 ◽

Vol 14 (19) ◽

pp. 5841

Author(s):

Noor Ul Huda Altaf ◽

Muhammad Yasin Naz ◽

Shazia Shukrullah ◽

Haq Nawaz Bhatti ◽

Muhammad Irfan ◽

...

Keyword(s):

Escherichia Coli ◽

Silver Nanoparticles ◽

Growth Parameters ◽

Low Cost ◽

Reduction Process ◽

Aureus Strain ◽

P Value ◽

Synthesis Parameters ◽

Resonance Band ◽

Plasma Reduction

Various conventional approaches have been reported for the synthesis of nanomaterials without optimizing the role of synthesis parameters. The unoptimized studies not only raise the process cost but also complicate the physicochemical characteristics of the nanostructures. The liquid–plasma reduction with optimized synthesis parameters is an environmentally friendly and low-cost technique for the synthesis of a range of nanomaterials. This work is focused on the statistically optimized production of silver nanoparticles (AgNPs) by using a liquid–plasma reduction process sustained with an argon plasma jet. A simplex centroid design (SCD) was made in Minitab statistical package to optimize the combined effect of stabilizers on the structural growth and UV absorbance of AgNPs. Different combinations of glucose, fructose, sucrose and lactose stabilizers were tested at five different levels (−2, −1, 0, 1, 2) in SCD. The effect of individual and mixed stabilizers on AgNPs growth parameters was assumed significant when p-value in SCD is less than 0.05. A surface plasmon resonance band was fixed at 302 nm after SCD optimization of UV results. A bond stretching at 1633 cm−1 in FTIR spectra was assigned to C=O, which slightly shifts towards a larger wavelength in the presence of saccharides in the solution. The presence of FCC structured AgNPs with an average size of 15 nm was confirmed from XRD and EDX spectra under optimized conditions. The antibacterial activity of these nanoparticles was checked against Staphylococcus aureus and Escherichia coli strains by adopting the shake flask method. The antibacterial study revealed the slightly better performance of AgNPs against Staph. aureus strain than Escherichia coli.

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Antimicrobial Activity of Silver Nanoparticles Prepared Under an Ultrasonic Field

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2011.4.3.8 ◽

2011 ◽

Vol 4 (3) ◽

pp. 1491-1496

Author(s):

Umadevi M ◽

Rani T ◽

Balakrishnan T ◽

Ramanibai R

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Reduction Method ◽

Therapeutic Potential ◽

Chemical Reduction ◽

Ultrasonic Field ◽

Great Promise ◽

Chemical Reduction Method ◽

E Coli

Nanotechnology has great promise for improving the therapeutic potential of medicinal molecules and related agents. In this study, silver nanoparticles of different sizes were synthesized in an ultrasonic field using the chemical reduction method with sodium borohydride as a reducing agent. The size effect of silver nanoparticles on antimicrobial activity were tested against the microorganisms Staphylococcus aureus (MTCC No. 96), Bacillus subtilis (MTCC No. 441), Streptococcus mutans (MTCC No. 497), Escherichia coli (MTCC No. 739) and Pseudomonas aeruginosa (MTCC No. 1934). The results shows that B. subtilis, and E. coli were more sensitive to silver nanoparticles and its size, indicating the superior antimicrobial efficacy of silver nanoparticles.

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Robust immobilization of anionic silver nanoparticles on cellulose filter paper toward a low-cost point-of-use water disinfection system with improved anti-biofouling properties

RSC Advances ◽

10.1039/d0ra09152a ◽

2021 ◽

Vol 11 (9) ◽

pp. 4873-4882

Author(s):

Gongyan Liu ◽

Ruiquan Yu ◽

Jing Jiang ◽

Zhuang Ding ◽

Jing Ma ◽

...

Keyword(s):

Silver Nanoparticles ◽

Filter Paper ◽

Low Cost ◽

Water Disinfection ◽

Point Of Use ◽

Cellulose Filter

Point-of-use water disinfection by GA@AgNPs-LA-FP.

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Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles

BMC Microbiology ◽

10.1186/s12866-021-02097-2 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Abdulkader Masri ◽

Naveed Ahmed Khan ◽

Muhammad Zarul Hanifah Md Zoqratt ◽

Qasim Ayub ◽

Ayaz Anwar ◽

...

Keyword(s):

Escherichia Coli ◽

Silver Nanoparticles ◽

Minimum Inhibitory Concentration ◽

Inhibitory Concentration ◽

Neonatal Meningitis ◽

E Coli ◽

Drug Candidates ◽

Metabolic Genes ◽

Genetic Mechanisms ◽

Cell Stress Response

Abstract Backgrounds Escherichia coli K1 causes neonatal meningitis. Transcriptome studies are indispensable to comprehend the pathology and biology of these bacteria. Recently, we showed that nanoparticles loaded with Hesperidin are potential novel antibacterial agents against E. coli K1. Here, bacteria were treated with and without Hesperidin conjugated with silver nanoparticles, and silver alone, and 50% minimum inhibitory concentration was determined. Differential gene expression analysis using RNA-seq, was performed using Degust software and a set of genes involved in cell stress response and metabolism were selected for the study. Results 50% minimum inhibitory concentration with silver-conjugated Hesperidin was achieved with 0.5 μg/ml of Hesperidin conjugated with silver nanoparticles at 1 h. Differential genetic analysis revealed the expression of 122 genes (≥ 2-log FC, P< 0.01) in both E. coli K1 treated with Hesperidin conjugated silver nanoparticles and E. coli K1 treated with silver alone, compared to untreated E. coli K1. Of note, the expression levels of cation efflux genes (cusA and copA) and translocation of ions, across the membrane genes (rsxB) were found to increase 2.6, 3.1, and 3.3- log FC, respectively. Significant regulation was observed for metabolic genes and several genes involved in the coordination of flagella. Conclusions The antibacterial mechanism of nanoparticles maybe due to disruption of the cell membrane, oxidative stress, and metabolism in E. coli K1. Further studies will lead to a better understanding of the genetic mechanisms underlying treatment with nanoparticles and identification of much needed novel antimicrobial drug candidates.

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Size-controlled fabrication of silver nanoparticles using the Hedyotis puberula leaf extract: toxicity on mosquito vectors and impact on biological control agents

RSC Advances ◽

10.1039/c6ra23208f ◽

2016 ◽

Vol 6 (99) ◽

pp. 96573-96583 ◽

Cited By ~ 7

Author(s):

Raja Mohamed Sait Thameem Azarudeen ◽

Marimuthu Govindarajan ◽

Abubucker Amsath ◽

Shine Kadaikunnan ◽

Naiyf S. Alharbi ◽

...

Keyword(s):

Biological Control ◽

Silver Nanoparticles ◽

Leaf Extract ◽

Low Cost ◽

Aquatic Organisms ◽

Capping Agent ◽

Mosquito Vectors ◽

Biological Control Agents ◽

Size Controlled ◽

Control Tool

As a low-cost and eco-friendly control tool, Ag nanoparticles were fabricated usingHedyotis puberulaaqueous extract as a reducing and capping agent and showed potent activity against malaria and arbovirus vectors with low biotoxicity against non-target aquatic organisms.

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Chitosan/poly(vinyl alcohol)/bovine bone powder biocomposites: A potential biomaterial for the treatment of atopic dermatitis-like skin lesions

Carbohydrate Polymers ◽

10.1016/j.carbpol.2016.04.049 ◽

2016 ◽

Vol 148 ◽

pp. 115-124 ◽

Cited By ~ 21

Author(s):

Nátali O. Alves ◽

Gabriela T. da Silva ◽

Douglas M. Weber ◽

Cristiane Luchese ◽

Ethel A. Wilhelm ◽

...

Keyword(s):

Atopic Dermatitis ◽

Vinyl Alcohol ◽

Skin Lesions ◽

Poly Vinyl Alcohol ◽

Bovine Bone ◽

Bone Powder

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Immobilization of Silver Nanoparticles on Chitosan-Coated Silica-Gel-Beads and the Antibacterial Activity

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.892.36 ◽

2021 ◽

Vol 892 ◽

pp. 36-42

Author(s):

Muhammad Iqbal Hidayat ◽

Muhammad Adlim ◽

Ilham Maulana ◽

Muhammad Zulfajri

Keyword(s):

Silver Nanoparticles ◽

Silica Gel ◽

Low Cost ◽

Antibacterial Properties ◽

Chitosan Film ◽

Silver Ions ◽

Filter Material ◽

Silver Ion ◽

Gel Beads ◽

Metal Colloid

Silver nanoparticles (Ag0) have attracted the most attention due to their broad antimicrobial application and outstanding activity. The silver nanoparticles are usually in colloidal form, then immobilization the colloid onto solid support is still interesting to explore. In this work, a new method for silver colloidal nanoparticle immobilization on silica gel beads (SiG), which was then symbolized as Ag0-[chi-SiG] was conducted and characterized successfully. The finding proved that SiG must be coated with three chitosan film layers to give stable support for silver nanoparticles. This coating method caused the chitosan completely covered SiG, and the chitosan film provides coordination bonding for silver ions. The most appropriate solvent for silver ion impregnation on the surface of chi-SiG is methanol compared to other solvents. Tungsten lamp as the photo-irradiation, which is low cost and environmentally friendly has been proven effective for silver ion reduction, as shown by silver metal colloid UV-Vis surface plasmon resonance at 400-700 nm. Ag0-[chi-SiG] showed the antibacterial properties of inhibiting the growth Staphylococcus aureus and Escherichia coli; then it provides the potential application for antibacterial filter material. According to the weight comparison between antibacterial standard and Ag content, then Ag0-[chi-SiG] has two and five times higher of exhibiting zone for each bacteria.

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Low Cost Controlled Composting of Refuse and Sewage Sludge

Water Science & Technology ◽

10.2166/wst.1987.0262 ◽

1987 ◽

Vol 19 (5-6) ◽

pp. 839-845 ◽

Cited By ~ 5

Author(s):

J. T. Pereira-Neto ◽

E. I. Stentiford ◽

D. D. Mara

Keyword(s):

Escherichia Coli ◽

Sewage Sludge ◽

Low Cost ◽

Control Methods ◽

Fixed Rate ◽

The Past ◽

Temperature Feedback ◽

Forced Aeration ◽

Domestic Refuse ◽

Faecal Streptococci

The forced aeration static pile composting system was used to compost mixtures of domestic refuse and sewage sludge. Several different control methods have been evaluated over the past four years from simple, low cost fixed rate aeration timers to microcomputer based systems. Their relative merits are considered. In a compost pile using temperature feedback control the number of Escherichia coli were reduced from 107 org./g to less than 102 org./g. within 16 days. Faecal streptococci were reduced from 107 to less than 102 org./g within 30 days. The process consistently produced a good quality sanitised material under a range of control regimes.

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