scholarly journals Statistically Optimized Production of Saccharides Stabilized Silver Nanoparticles Using Liquid–Plasma Reduction Approach for Antibacterial Treatment of Water

Materials ◽  
2021 ◽  
Vol 14 (19) ◽  
pp. 5841
Author(s):  
Noor Ul Huda Altaf ◽  
Muhammad Yasin Naz ◽  
Shazia Shukrullah ◽  
Haq Nawaz Bhatti ◽  
Muhammad Irfan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Growth Parameters ◽  
Low Cost ◽  
Reduction Process ◽  
Aureus Strain ◽  
P Value ◽  
Synthesis Parameters ◽  
Resonance Band ◽  
Plasma Reduction

Various conventional approaches have been reported for the synthesis of nanomaterials without optimizing the role of synthesis parameters. The unoptimized studies not only raise the process cost but also complicate the physicochemical characteristics of the nanostructures. The liquid–plasma reduction with optimized synthesis parameters is an environmentally friendly and low-cost technique for the synthesis of a range of nanomaterials. This work is focused on the statistically optimized production of silver nanoparticles (AgNPs) by using a liquid–plasma reduction process sustained with an argon plasma jet. A simplex centroid design (SCD) was made in Minitab statistical package to optimize the combined effect of stabilizers on the structural growth and UV absorbance of AgNPs. Different combinations of glucose, fructose, sucrose and lactose stabilizers were tested at five different levels (−2, −1, 0, 1, 2) in SCD. The effect of individual and mixed stabilizers on AgNPs growth parameters was assumed significant when p-value in SCD is less than 0.05. A surface plasmon resonance band was fixed at 302 nm after SCD optimization of UV results. A bond stretching at 1633 cm−1 in FTIR spectra was assigned to C=O, which slightly shifts towards a larger wavelength in the presence of saccharides in the solution. The presence of FCC structured AgNPs with an average size of 15 nm was confirmed from XRD and EDX spectra under optimized conditions. The antibacterial activity of these nanoparticles was checked against Staphylococcus aureus and Escherichia coli strains by adopting the shake flask method. The antibacterial study revealed the slightly better performance of AgNPs against Staph. aureus strain than Escherichia coli.

scholarly journals Synthesis of Silver Nanoparticles by Phyllanthus emblica Plant Extract and Their Antibacterial Activity

2020 ◽  
Vol 17 (2) ◽  
pp. 136-145
Author(s):  
Rajesh Kumar Meena ◽  
Risikesh Meena ◽  
Dinesh Kumar Arya ◽  
Sapana Jadoun ◽  
Renu Hada ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Plant Extract ◽  
Low Cost ◽  
Green Technology ◽  
Phyllanthus Emblica ◽  
Visible Spectroscopy ◽  
Synthesis Of Nanoparticles ◽  
Resonance Band ◽  
Uv Visible

The silver nanoparticle was successfully synthesized by using the help of Phyllanthus emblica plant extract as a reducing agent and aqueous silver nitrate as the precursor. Moreover, physical and chemical methods are widely used for the synthesis of nanoparticles, but these methods have expensive and not ecofriendly. This study highlights the green, rapid, facile, cost-effective, and ecofriendly synthesis and synthesized nanoparticles also investigate their antibacterial activity. Synthesized silver nanoparticles are analyzed by different techniques of modes like XRD, UV-Visible spectroscopy, TEM, FTIR, and photoluminescence (PL). The prepared AgNPs show characteristic absorption peak in UV-Visible spectroscopy due to SPR (surface plasmonic resonance) band between 400 to 450 nm wavelength, which was confirmed by TEM (transmission electron microscopy) image. X-ray diffraction (XRD) results showed the crystalline nature of AgNPs as well as the size of nanoparticles calculated with the help of TEM (20-25 nm) and XRD (25 nm). ATR spectroscopy identified the functional groups that are involved in the reduction of silver ion to AgNPs and the PL spectrum indicates higher emission in the green region and low emission peak in the UV region. Antibacterial activity of AgNPs analyzed against with the help of E.Coli bacteria and the result shows that a higher concentration of AgNPs is increasing as well as a zone of inhibition increased. This method is environmentally friendly, of low cost, and less expensive method for the fabrication of AgNPs in abundance which can be further helpful for biosensor devices as well as for other applications such as pollutant degradation, pharmaceutical, and hydrogen production, etc therefore can promote the application of green technology for the production of AgNPs.

scholarly journals Bionanotechnology: Silver Nanoparticles Supported on Bovine Bone Powder Used as Bactericide

Materials ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 462
Author(s):  
Sergio Arturo Gama-Lara ◽  
Martha Stephanie Pérez Mendoza ◽  
Alfredo Rafael Vilchis-Nestor ◽  
Reyna Natividad
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Low Cost ◽  
Bovine Bone ◽  
Salt Reduction ◽  
Decimal Reduction Time ◽  
Weight Content ◽  
Metallic Salt ◽  
Water As Solvent ◽  
Bone Powder

Bionanotechnology is a relatively new term that implies the use of some biological material or organisms in order to prepare nanosystems or nanoparticles. This work presents the preparation and bactericide application of a sustainable nanometric system (silver nanoparticles) using a waste biological support (bovine bone powder). This system was prepared by the method of metallic salt reduction, using NaBH4 as reducing agent and AgNO3 as metallic salt. Two silver contents were analyzed, 1% and 5% weight. The latter was found to be more efficient than the former. Transmission electronic microscopy shows an average size of 10.5 ± 3.3 nm and quasi-sphere morphology. The antimicrobial assay shows that a 5% weight content of silver had a bactericide effect for Escherichia coli at 46.8 min of exposure. The minimum inhibitory concentration (MIC) of silver nanoparticles supported on bovine bone powder for Escherichia coli was 7.5 µg/mL. The biocomposite exhibits a specific antibacterial kinetics constant (k) of 0.1128 min−1 and decimal reduction time (DRT) of 20.39 min for Escherichia coli. Thus, it was concluded that a biocomposite was prepared with a biodegradable, waste, and low-cost support, under mild conditions (room temperature and atmospheric pressure) and using water as solvent.

Antimicrobial Activity of Silver Nanoparticles Prepared Under an Ultrasonic Field

2011 ◽  
Vol 4 (3) ◽  
pp. 1491-1496
Author(s):  
Umadevi M ◽  
Rani T ◽  
Balakrishnan T ◽  
Ramanibai R
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Reduction Method ◽  
Therapeutic Potential ◽  
Chemical Reduction ◽  
Ultrasonic Field ◽  
Great Promise ◽  
Chemical Reduction Method ◽  
E Coli

Nanotechnology has great promise for improving the therapeutic potential of medicinal molecules and related agents. In this study, silver nanoparticles of different sizes were synthesized in an ultrasonic field using the chemical reduction method with sodium borohydride as a reducing agent. The size effect of silver nanoparticles on antimicrobial activity were tested against the microorganisms Staphylococcus aureus (MTCC No. 96), Bacillus subtilis (MTCC No. 441), Streptococcus mutans (MTCC No. 497), Escherichia coli (MTCC No. 739) and Pseudomonas aeruginosa (MTCC No. 1934). The results shows that B. subtilis, and E. coli were more sensitive to silver nanoparticles and its size, indicating the superior antimicrobial efficacy of silver nanoparticles. 

Testing of photocatalytic potential of silver nanoparticles produced through nonthermal plasma reduction reaction and stabilized with saccharides

2021 ◽  
pp. 1-14
Author(s):  
N.U.H. Altaf ◽  
M.Y. Naz ◽  
S. Shukrullah ◽  
H.N. Bhatti
Keyword(s):  
Silver Nanoparticles ◽  
Band Gap ◽  
Light Exposure ◽  
Argon Plasma ◽  
Silver Ions ◽  
Reduction Reaction ◽  
Diameter Distribution ◽  
Light Spectrum ◽  
Plasma Reduction ◽  
Narrow Diameter Distribution

In this study, silver nanoparticles (AgNPs) were produced through an atmospheric pressure plasma reduction reaction and tested for photodegradation of methyl blue (MB) under sunlight exposure. The argon plasma born reactive species were used to reduce silver ions to AgNPs in the solution. Glucose, fructose and sucrose were also added in the solution to stabilize the growth process. The glucose stabilized reaction produced the smallest nanoparticles of 12 nm, while sucrose stabilized reaction produced relatively larger nanoparticles (14 nm). The nanoparticles exhibited rough morphology and narrow diameter distribution regardless of stabilizer type. The narrow diameter distribution and small band gap helped activating majority of nanoparticles at a single wavelength of light spectrum. The band gap energy of AgNPs varied from 2.22 eV to 2.41 eV, depending on the saccharide type. The photoluminescence spectroscopy of AgNPs produced emission peaks at 413 nm, 415 nm, and 418 nm. The photocatalytic potential of AgNP samples was checked by degrading MB dye under sunlight. The degradation reaction reached a saturation level of 98% after 60 min of light exposure.

scholarly journals Facile Synthesis of Carboxymethyl Cellulose Coated Core/Shell SiO2@Cu Nanoparticles and Their Antifungal Activity against Phytophthora capsici

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 888
Author(s):  
Nguyen Thi Thanh Hai ◽  
Nguyen Duc Cuong ◽  
Nguyen Tran Quyen ◽  
Nguyen Quoc Hien ◽  
Tran Thi Dieu Hien ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Carboxymethyl Cellulose ◽  
Chemical Reduction ◽  
Low Cost ◽  
Phytophthora Capsici ◽  
Reduction Process ◽  
Spherical Shape ◽  
Core Shell ◽  
Cu Nanoparticles ◽  
Average Size

Cu nanoparticles are a potential material for creating novel alternative antimicrobial products due to their unique antibacterial/antifungal properties, stability, dispersion, low cost and abundance as well as being economical and ecofriendly. In this work, carboxymethyl cellulose coated core/shell SiO2@Cu nanoparticles (NPs) were synthesized by a simple and effective chemical reduction process. The initial SiO2 NPs, which were prepared from rice husk ash, were coated by a copper ultrathin film using hydrazine and carboxymethyl cellulose (CMC) as reducing agent and stable agent, respectively. The core/shell SiO2@Cu nanoparticles with an average size of ~19 nm were surrounded by CMC. The results indicated that the SiO2@Cu@CMC suspension was a homogenous morphology with a spherical shape, regular dispersion and good stability. Furthermore, the multicomponent SiO2@Cu@CMC NPs showed good antifungal activity against Phytophthora capsici (P. capsici). The novel Cu NPs-based multicomponent suspension is a key compound in the development of new fungicides for the control of the Phytophthora disease.

scholarly journals Robust immobilization of anionic silver nanoparticles on cellulose filter paper toward a low-cost point-of-use water disinfection system with improved anti-biofouling properties

RSC Advances ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 4873-4882
Author(s):  
Gongyan Liu ◽  
Ruiquan Yu ◽  
Jing Jiang ◽  
Zhuang Ding ◽  
Jing Ma ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Filter Paper ◽  
Low Cost ◽  
Water Disinfection ◽  
Point Of Use ◽  
Cellulose Filter

Point-of-use water disinfection by GA@AgNPs-LA-FP.

scholarly journals Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Abdulkader Masri ◽  
Naveed Ahmed Khan ◽  
Muhammad Zarul Hanifah Md Zoqratt ◽  
Qasim Ayub ◽  
Ayaz Anwar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Neonatal Meningitis ◽  
E Coli ◽  
Drug Candidates ◽  
Metabolic Genes ◽  
Genetic Mechanisms ◽  
Cell Stress Response

Abstract Backgrounds Escherichia coli K1 causes neonatal meningitis. Transcriptome studies are indispensable to comprehend the pathology and biology of these bacteria. Recently, we showed that nanoparticles loaded with Hesperidin are potential novel antibacterial agents against E. coli K1. Here, bacteria were treated with and without Hesperidin conjugated with silver nanoparticles, and silver alone, and 50% minimum inhibitory concentration was determined. Differential gene expression analysis using RNA-seq, was performed using Degust software and a set of genes involved in cell stress response and metabolism were selected for the study. Results 50% minimum inhibitory concentration with silver-conjugated Hesperidin was achieved with 0.5 μg/ml of Hesperidin conjugated with silver nanoparticles at 1 h. Differential genetic analysis revealed the expression of 122 genes (≥ 2-log FC, P< 0.01) in both E. coli K1 treated with Hesperidin conjugated silver nanoparticles and E. coli K1 treated with silver alone, compared to untreated E. coli K1. Of note, the expression levels of cation efflux genes (cusA and copA) and translocation of ions, across the membrane genes (rsxB) were found to increase 2.6, 3.1, and 3.3- log FC, respectively. Significant regulation was observed for metabolic genes and several genes involved in the coordination of flagella. Conclusions The antibacterial mechanism of nanoparticles maybe due to disruption of the cell membrane, oxidative stress, and metabolism in E. coli K1. Further studies will lead to a better understanding of the genetic mechanisms underlying treatment with nanoparticles and identification of much needed novel antimicrobial drug candidates.

scholarly journals Size Control of Synthesized Silver Nanoparticles by Simultaneous Chemical Reduction and Laser Fragmentation in Origanum majorana Extract: Antibacterial Application

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2326
Author(s):  
Entesar Ali Ganash ◽  
Reem Mohammad Altuwirqi
Keyword(s):  
Silver Nanoparticles ◽  
Irradiation Time ◽  
Chemical Reduction ◽  
Size Control ◽  
Reduction Process ◽  
Laser Wavelength ◽  
X Ray Diffraction ◽  
Ag Nps ◽  
Transmission Electron ◽  
Origanum Majorana

In this work, silver nanoparticles (Ag NPs) were synthesized using a chemical reduction approach and a pulsed laser fragmentation in liquid (PLFL) technique, simultaneously. A laser wavelength of 532 nm was focused on the as produced Ag NPs, suspended in an Origanum majorana extract solution, with the aim of controlling their size. The effect of liquid medium concentration and irradiation time on the properties of the fabricated NPs was studied. While the X-ray diffraction (XRD) pattern confirmed the existence of Ag NPs, the UV–Vis spectrophotometry showed a significant absorption peak at about 420 nm, which is attributed to the characteristic surface plasmon resonance (SPR) peak of the obtained Ag NPs. By increasing the irradiation time and the Origanum majora extract concentration, the SPR peak shifted toward a shorter wavelength. This shift indicates a reduction in the NPs’ size. The effect of PLFL on size reduction was clearly revealed from the transmission electron microscopy images. The PLFL technique, depending on experimental parameters, reduced the size of the obtained Ag NPs to less than 10 nm. The mean zeta potential of the fabricated Ag NPs was found to be greater than −30 mV, signifying their stability. The Ag NPs were also found to effectively inhibit bacterial activity. The PLFL technique has proved to be a powerful method for controlling the size of NPs when it is simultaneously associated with a chemical reduction process.

scholarly journals Characterization of Escherichia coli Strains Derived from Cow Milk of Subclinical and Clinical Cases of Mastitis

2021 ◽  
Vol 11 (2) ◽  
pp. 541
Author(s):  
Katarzyna Grudlewska-Buda ◽  
Krzysztof Skowron ◽  
Ewa Wałecka-Zacharska ◽  
Natalia Wiktorczyk-Kapischke ◽  
Jarosław Bystroń ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacterial Species ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Economic Problem ◽  
Cow Milk ◽  
P Value ◽  
Dairy Herds ◽  
E Coli ◽  
Bonferroni Test

Mastitis is a major economic problem in dairy herds, as it might decrease fertility, and negatively affect milk quality and milk yield. Out of over 150 bacterial species responsible for the udder inflammation, Escherichia coli is one of the most notable. This study aimed to assess antimicrobial susceptibility, resistance to dipping agents and biofilm formation of 150 E. coli strains isolated from milk of cows with subclinical and clinical mastitis. The strains came from three dairy herds located in Northern and Central Poland. The statistical analyses were performed with post-hoc Bonferroni test and chi-square test (including Yates correction). The data with a p value of <0.05 were considered significant. We found that the tested strains were mostly sensitive to antimicrobials and dipping agents. It was shown that 37.33% and 4.67% of strains were resistant and moderately resistant to at least one antimicrobial agent, respectively. No extended-spectrum beta-lactamases (ESBL)-producing E. coli were detected. The majority of strains did not possess the ability to form biofilm or formed a weak biofilm. The strong biofilm formers were found only among strains derived from cows with subclinical mastitis. The lowest bacteria number was noted for subclinical mastitis cows’ strains, after stabilization with iodine (3.77 log CFU × cm−2) and chlorhexidine (3.96 log CFU × cm−2) treatment. In the present study, no statistically significant differences in susceptibility to antibiotics and the ability to form biofilm were found among the strains isolated from cows with subclinical and clinical mastitis. Despite this, infections in dairy herds should be monitored. Limiting the spread of bacteria and characterizing the most common etiological factors would allow proper treatment.

Size-controlled fabrication of silver nanoparticles using the Hedyotis puberula leaf extract: toxicity on mosquito vectors and impact on biological control agents

RSC Advances ◽  
2016 ◽  
Vol 6 (99) ◽  
pp. 96573-96583 ◽  
Author(s):  
Raja Mohamed Sait Thameem Azarudeen ◽  
Marimuthu Govindarajan ◽  
Abubucker Amsath ◽  
Shine Kadaikunnan ◽  
Naiyf S. Alharbi ◽  
...  
Keyword(s):  
Biological Control ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Low Cost ◽  
Aquatic Organisms ◽  
Capping Agent ◽  
Mosquito Vectors ◽  
Biological Control Agents ◽  
Size Controlled ◽  
Control Tool

As a low-cost and eco-friendly control tool, Ag nanoparticles were fabricated usingHedyotis puberulaaqueous extract as a reducing and capping agent and showed potent activity against malaria and arbovirus vectors with low biotoxicity against non-target aquatic organisms.

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