scholarly journals The Effect of Liquid Rubber Addition on the Physicochemical Properties, Cytotoxicity and Ability to Inhibit Biofilm Formation of Dental Composites

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1704
Author(s):  
Krzysztof Pałka ◽  
Małgorzata Miazga-Karska ◽  
Joanna Pawłat ◽  
Joanna Kleczewska ◽  
Agata Przekora
Keyword(s):  
Cell Viability ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Type Material ◽  
Dental Composites ◽  
Antibiofilm Activity ◽  
Ceramic Filler ◽  
Liquid Rubber ◽  
Flow Type ◽  
Type Composite

The aim of this study was to evaluate the effect of modification with liquid rubber on the adhesion to tooth tissues (enamel, dentin), wettability and ability to inhibit bacterial biofilm formation of resin-based dental composites. Two commercial composites (Flow-Art–flow type with 60% ceramic filler and Boston–packable type with 78% ceramic filler; both from Arkona Laboratorium Farmakologii Stomatologicznej, Nasutów, Poland) were modified by addition of 5% by weight (of resin) of a liquid methacrylate-terminated polybutadiene. Results showed that modification of the flow type composite significantly (p < 0.05) increased the shear bond strength values by 17% for enamel and by 33% for dentine. Addition of liquid rubber significantly (p < 0.05) reduced also hydrophilicity of the dental materials since the water contact angle was increased from 81–83° to 87–89°. Interestingly, modified packable type material showed improved antibiofilm activity against Steptococcus mutans and Streptococcus sanguinis (quantitative assay with crystal violet), but also cytotoxicity against eukaryotic cells since cell viability was reduced to 37% as proven in a direct-contact WST-8 test. Introduction of the same modification to the flow type material significantly improved its antibiofilm properties (biofilm reduction by approximately 6% compared to the unmodified material, p < 0.05) without cytotoxic effects against human fibroblasts (cell viability near 100%). Thus, modified flow type composite may be considered as a candidate to be used as restorative material since it exhibits both nontoxicity and antibiofilm properties.

scholarly journals The Antibiofilm Activity of Dual-Function Tail Tubular Protein B from KP32 Phage

2018 ◽  
Author(s):  
Ewa Brzozowska ◽  
Anna Pyra ◽  
Krzysztof Pawlik ◽  
Sabina Górska ◽  
Andrzej Gamian
Keyword(s):  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Biofilm Formation ◽  
Hydrolytic Activity ◽  
Bacterial Biofilm ◽  
Dual Function ◽  
Antibiofilm Activity ◽  
Synergistic Activity ◽  
Antibiotic Action ◽  
Phage Protein

Background: Dual function tail tubular proteins (TTP) belonging to the lytic bacteriophages are the interesting group of biologically active enzymes. Surprisingly, apart from their structural function, they are also polysaccharide hydrolyzes destroying bacterial extracellular components. One of the representatives of this group is TTPB from Klebsiella pneumoniae phage &ndash; KP32. TTPB hydrolyzes exopolysaccharide (EPS) of Klebsiella pneumoniae and Enterococcus faecalis strain. This depolymerizing feature was associated with the activity to prevent bacterial biofilm formation. TTPB can inhibit biofilm formation by K. pneumoniae, Enterobacter cloacae, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa strains. Moreover, synergistic activity with antibiotic action has been observed, most likely due to depolymerases&rsquo; facilitation of contact of antibiotic with bacterial cells. Methods: TTPB was overexpressed in E coli system, purified and tested towards the bacterial strains using agar overlay method. The hydrolytic activity of TTPB was performed using EPSs of K. pneumoniae PCM2713 and E. cloacae ATCC 13047 as the substrates. Next, we determined the reducing sugar (RS) levels in the TTPB/EPS mixtures, regarding the RS amount obtained after acidic hydrolysis. The antibiofilm activity of TTPB has been set down on bacterial biofilm using a biochemical method. Finally, we have demonstrated the synergistic activity of TTPB with kanamycin. Results: For the first time, the hydrolytic activity of TTPB towards bacterial EPSs has been shown. TTPB releases about a half of the whole RS amount of EPSs belonging to K. pneumoniae PCM 2713 and E. cloacae ATCC 13047 strains. 1.12 &micro;M of the phage protein reduces biofilm of both strains by over 60%. Destroying the bacterial biofilm the phage protein improves the antibiotic action increasing kanamycin effectiveness up to four times.

scholarly journals Improved Fracture Toughness and Conversion Degree of Resin-Based Dental Composites after Modification with Liquid Rubber

Materials ◽  
2020 ◽  
Vol 13 (12) ◽  
pp. 2704
Author(s):  
Krzysztof Pałka ◽  
Joanna Kleczewska ◽  
Emil Sasimowski ◽  
Anna Belcarz ◽  
Agata Przekora
Keyword(s):  
Mechanical Properties ◽  
Fracture Toughness ◽  
Dental Materials ◽  
Degree Of Conversion ◽  
Dental Composites ◽  
Conversion Degree ◽  
Matrix Resin ◽  
Ceramic Filler ◽  
Liquid Rubber ◽  
The Matrix

There are many methods widely applied in the engineering of biomaterials to improve the mechanical properties of the dental composites. The aim of this study was to assess the effect of modification of dental composites with liquid rubber on their mechanical properties, degree of conversion, viscosity, and cytotoxicity. Both flow and packable composite consisted of a mixture of Bis-GMA, TEGDMA, UDMA, and EBADMA resins reinforced with 60 and 78 wt.% ceramic filler, respectively. It was demonstrated that liquid rubber addition significantly increased the fracture toughness by 9% for flow type and 8% for condensable composite. The influence of liquid rubber on flexural strength was not statistically significant. The addition of the toughening agent significantly reduced Young’s modulus by 7% and 9%, respectively, while increasing deformation at breakage. Scanning electron microscopy (SEM) observations allowed to determine the mechanisms of toughening the composites reinforced with ceramic particles. These mechanisms included bridging the crack edges, blocking the crack tip by particles and dissipation of fracture energy by deflection of the cracks on larger particles. The degree of conversion increased after modification, mainly due to a decrease in the matrix resin viscosity. It was also shown that all dental materials were nontoxic according to ISO 10993-5, indicating that modified materials have great potential for commercialization and clinical applications.

Antibiofilm activity and cytotoxicity of silk sericin against Streptococcus mutans bacteria in biofilm: an in vitro study

2020 ◽  
Vol 29 (Sup4) ◽  
pp. S25-S35
Author(s):  
Pornanong Aramwit ◽  
Supamas Napavichayanum ◽  
Prompong Pienpinijtham ◽  
Yousef Rasmi ◽  
Nipaporn Bang
Keyword(s):  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
In Vitro Study ◽  
Bacterial Biofilm ◽  
Vitro Study ◽  
Antibiofilm Activity ◽  
Diphenyltetrazolium Bromide ◽  
Infrared Spectrometer ◽  
Disruption Effect

Objective: To investigate the potential of sericin extracted by different methods to inhibit biofilm formation (prevention) and disrupt already formed biofilm (treatment). Method: In this in vitro study, sericin was extracted by heat, acid, alkali and urea. Streptococcus mutans bacteria were cultivated in the presence of various concentrations of sericin to evaluate antibiofilm formation using cell density assay (inhibition effect before biofilm formed). Conversely, various concentrations of sericin were added to a biofilm already formed by Streptococcus mutans bacteria, and the viability of bacteria assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (disruption effects after biofilm formed). Structures of extracted sericin were evaluated using circular dichroism and Fourier-transform infrared spectrometer. Results: The urea-extracted sericin at all concentrations (12.5mg/ml, 25mg/ml, 50mg/ml and 100mg/ml) showed the highest potential antibiofilm activity in terms of both inhibition and disruption effects, compared with sericin extracted by heat, acid or alkali. The heat-extracted and acid-extracted sericin were found to reduce the biofilm formation dose-dependently, while the alkali-extracted sericin did not show either inhibition or disruption effect on the bacterial biofilm. The urea-extracted sericin also killed the bacteria residing within the biofilm, possibly due to its modified structure which may destabilise the bacterial cell wall, leading to membrane disintegration and, finally, cell death. Conclusion: Our results demostrated the antibiofilm activity of sericin. This could form the basis of further research on the mechanism and application of sericin as a novel antibiofilm agent.

scholarly journals Derivatives of the Mouse Cathelicidin-Related Antimicrobial Peptide (CRAMP) Inhibit Fungal and Bacterial Biofilm Formation

2014 ◽  
Vol 58 (9) ◽  
pp. 5395-5404 ◽  
Author(s):  
Katrijn De Brucker ◽  
Nicolas Delattin ◽  
Stijn Robijns ◽  
Hans Steenackers ◽  
Natalie Verstraeten ◽  
...  
Keyword(s):  
Amino Acid ◽  
Antimicrobial Peptide ◽  
Inhibitory Activity ◽  
Biofilm Formation ◽  
Specific Activity ◽  
Cell Types ◽  
Bacterial Biofilm ◽  
Antibiofilm Activity ◽  
Content Type ◽  
Derivatives Of

ABSTRACTWe identified a 26-amino-acid truncated form of the 34-amino-acid cathelicidin-related antimicrobial peptide (CRAMP) in the islets of Langerhans of the murine pancreas. This peptide, P318, shares 67% identity with the LL-37 human antimicrobial peptide. As LL-37 displays antimicrobial and antibiofilm activity, we tested antifungal and antibiofilm activity of P318 against the fungal pathogenCandida albicans. P318 shows biofilm-specific activity as it inhibitsC. albicansbiofilm formation at 0.15 μM without affecting planktonic survival at that concentration. Next, we tested theC. albicansbiofilm-inhibitory activity of a series of truncated and alanine-substituted derivatives of P318. Based on the biofilm-inhibitory activity of these derivatives and the length of the peptides, we decided to synthesize the shortened alanine-substituted peptide at position 10 (AS10; KLKKIAQKIKNFFQKLVP). AS10 inhibitedC. albicansbiofilm formation at 0.22 μM and acted synergistically with amphotericin B and caspofungin against mature biofilms. AS10 also inhibited biofilm formation of different bacteria as well as of fungi and bacteria in a mixed biofilm. In addition, AS10 does not affect the viability or functionality of different cell types involved in osseointegration of an implant, pointing to the potential of AS10 for further development as a lead peptide to coat implants.

scholarly journals Small-Molecule Compound SYG-180-2-2 to Effectively Prevent the Biofilm Formation of Methicillin-Resistant Staphylococcus aureus

2022 ◽  
Vol 12 ◽  
Author(s):  
Lulin Rao ◽  
Yaoguang Sheng ◽  
Jiao Zhang ◽  
Yanlei Xu ◽  
Jingyi Yu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Small Molecule ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Alveolar Epithelial Cells ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Antibiofilm Activity ◽  
Methicillin Resistant ◽  
Small Molecule Compound

The resistance of methicillin-resistant Staphylococcus aureus (MRSA) has augmented due to the abuse of antibiotics, bringing about difficulties in the treatment of infection especially with the formation of biofilm. Thus, it is essential to develop antimicrobials. Here we synthesized a novel small-molecule compound, which we termed SYG-180-2-2 (C21H16N2OSe), that had antibiofilm activity. The aim of this study was to demonstrate the antibiofilm effect of SYG-180-2-2 against clinical MRSA isolates at a subinhibitory concentration (4 μg/ml). In this study, it was showed that significant suppression in biofilm formation occurred with SYG-180-2-2 treatment, the inhibition ranged between 65.0 and 85.2%. Subsequently, confocal laser scanning microscopy and a bacterial biofilm metabolism activity assay further demonstrated that SYG-180-2-2 could suppress biofilm. Additionally, SYG-180-2-2 reduced bacterial adhesion and polysaccharide intercellular adhesin (PIA) production. It was found that the expression of icaA and other biofilm-related genes were downregulated as evaluated by RT-qPCR. At the same time, icaR and codY were upregulated when biofilms were treated with SYG-180-2-2. Based on the above results, we speculate that SYG-180-2-2 inhibits the formation of biofilm by affecting cell adhesion and the expression of genes related to PIA production. Above all, SYG-180-2-2 had no toxic effects on human normal alveolar epithelial cells BEAS-2B. Collectively, the small-molecule compound SYG-180-2-2 is a safe and effective antibacterial agent for inhibiting MRSA biofilm.

scholarly journals Inhibition of Bacterial Biofilm Formation and Swarming Motility by a Small Synthetic Cationic Peptide

2012 ◽  
Vol 56 (5) ◽  
pp. 2696-2704 ◽  
Author(s):  
César de la Fuente-Núñez ◽  
Victoria Korolik ◽  
Manjeet Bains ◽  
Uyen Nguyen ◽  
Elena B. M. Breidenstein ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Cell System ◽  
Bacterial Biofilm ◽  
Burkholderia Cenocepacia ◽  
Antibiofilm Activity ◽  
Cationic Peptide ◽  
Small Peptides ◽  
Swarming Motility ◽  
Content Type ◽  
Cell Biomass

ABSTRACTBiofilms cause up to 80% of infections and are difficult to treat due to their substantial multidrug resistance compared to their planktonic counterparts. Based on the observation that human peptide LL-37 is able to block biofilm formation at concentrations below its MIC, we screened for small peptides with antibiofilm activity and identified novel synthetic cationic peptide 1037 of only 9 amino acids in length. Peptide 1037 had very weak antimicrobial activity, but at 1/30th the MIC the peptide was able to effectively prevent biofilm formation (>50% reduction in cell biomass) by the Gram-negative pathogensPseudomonas aeruginosaandBurkholderia cenocepaciaand Gram-positiveListeria monocytogenes. Using a flow cell system and a widefield fluorescence microscope, 1037 was shown to significantly reduce biofilm formation and lead to cell death in biofilms. Microarray and follow-up studies showed that, inP. aeruginosa, 1037 directly inhibited biofilms by reducing swimming and swarming motilities, stimulating twitching motility, and suppressing the expression of a variety of genes involved in biofilm formation (e.g., PA2204). Comparison of microarray data from cells treated with peptides LL-37 and 1037 enabled the identification of 11 commonP. aeruginosagenes that have a role in biofilm formation and are proposed to represent functional targets of these peptides. Peptide 1037 shows promise as a potential therapeutic agent against chronic, recurrent biofilm infections caused by a variety of bacteria.

scholarly journals Triterpene Derivatives as Relevant Scaffold for New Antibiofilm Drugs

Biomolecules ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 58 ◽  
Author(s):  
Gloria Narjara Santos da Silva ◽  
Muriel Primon-Barros ◽  
Alexandre José Macedo ◽  
Simone Cristina Baggio Gnoatto
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Mammalian Cells ◽  
Antibacterial Effect ◽  
Bacterial Biofilm ◽  
Antibiofilm Activity ◽  
Vero Cell Line ◽  
Diphenyltetrazolium Bromide ◽  
Green Monkey

New medicines for the treatment of bacterial biofilm formation are required. For thisreason, this study shows the in vitro activity of betulinic acid (BA), ursolic acid (UA) and their twentyderivatives against planktonic and biofilm cells (gram-positive bacterial pathogens: Enterococcusfaecalis, <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i>). We evaluated the antibiofilm activity(through the crystal violet method), as well as the antibacterial activity via absorbance (OD<sub>600</sub>) atconcentrations of 5, 25 and 100 μM. Likewise, the cytotoxicity of all compounds was evaluated on akidney African green monkey (VERO) cell line at the same concentration, by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) methodology. We verified for the first timewhether different groups at carbon 3 (C-3) of triterpenes may interfere in the antibiofilm activity withminimal or no antibacterial effect. After the screening of 22 compounds at three distinctconcentrations, we found antibiofilm activity for eight distinct derivatives without antibiotic effect.In particular, the derivative 2f, with an isopentanoyl ester at position C-3, was an antibiofilm activityagainst S. aureus without any effect upon mammalian cells.

scholarly journals Antibiofilm activity of aminopropanol derivatives against Pseudomonas aeruginosa

2018 ◽  
pp. 93-100
Author(s):  
D. M. Dudikova ◽  
Z. S. Suvorova ◽  
V. V. Nedashkivska ◽  
A. O. Sharova ◽  
M. L. Dronova ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Bacterial Biofilm ◽  
Antibiofilm Activity ◽  
Dependent Manner ◽  
Inhibition Activity ◽  
Chronic Infections ◽  
High Concentration ◽  
Biofilm Inhibition

Bacterial biofilm, particularly formed by Pseudomonas aeruginosa, are a cause of severe chronic infectious diseases. Bacteria within a biofilm are phenotypically more resistant to antibiotics and the macroorganism immune system, making it an important virulence factor for many microbes. The aminopropanol derivatives with adamantyl (KVM-97) and N-alkylaryl radicals (KVM-194, KVM-204, KVM-261, and KVM-262) were used as study object. The aim of this study was to investigate the antibiofilm activity of compounds on biofilm formation and on mature biofilm of P. aeruginosa. The effects of the aminopropanol derivatives on the biofilm mass were evaluated by using crystal violet assay. Ciprofloxacin, meropenem, ceftazidime, gentamicin were used as reference substances. Reported results demonstrate that all compounds displayed antibiofilm activity at the tested concentrations. Remarkable reduction in biofilm formation of P. aeruginosa was found after treatment with KVM-97, KVM-261 and KVM-262 in high concentration (5× MIC), biofilm inhibition activity were 84.3%, 90.5% and 83.3% respectively. After a treatment with KVM-204 at 250 μg/ml (5× MIC) 76.6% of the preformed 24-hr biofilms were destroyed. Furthermore, compounds KVM-97, KVM-194, and KVM-261 in both concentrations showed potent antibiofilm activity against the P. aeruginosa, inhibition activity values being between 56.7 and 65.7%. All tested compounds in dose-dependent manner exhibited pronounced inhibition activity against mature 5-days P. аeruginosa biofilm. It was also observed that tested compounds show high antibiofilm activity in comparison to reference antimicrobials. The aminopropanol derivatives may provide templates for a new group of antimicrobial agents and potential future therapeutics for treating chronic infections.

scholarly journals Controlling biofilm formation with nitroxide functional surfaces

2019 ◽  
Vol 10 (31) ◽  
pp. 4252-4258 ◽  
Author(s):  
Hendrik Woehlk ◽  
Michael J. Trimble ◽  
Sarah C. Mansour ◽  
Daniel Pletzer ◽  
Vanessa Trouillet ◽  
...  
Keyword(s):  
Medical Devices ◽  
Biofilm Formation ◽  
Polymer Coatings ◽  
Bacterial Biofilm ◽  
Antibiofilm Activity ◽  
Functional Polymer ◽  
Functional Surfaces

Nitroxide functional polymer coatings with inherent antibiofilm activity are introduced as an avenue to combat bacterial biofilm contamination of medical devices.

Simultaneous Antibacterial and Antibiofilm Activities of three Types of Silver Nanoparticles against Human Bacterial Pathogens.

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Suhad Hadi Mohammed ◽  
Maysaa Saleh Mahdi ◽  
Abbas Matrood Bashi ◽  
Mohanad Mohsin Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Significant Inhibition ◽  
Bacterial Biofilm ◽  
Resistant Bacteria ◽  
Antibiofilm Activity ◽  
Bacterial Cells ◽  
Dual Effect ◽  
Low Concentration

Emergence of antibiotic resistant bacteria result in an approximately 550,000 deaths from bacterial infections annually. Several studies reported interesting antibacterial and anti-biofilm activities of silver nanoparticles synthesized by different physical, chemical and biological methods as an alternative to antibiotics. However, the resultant nanoparticles were varying in size, shape, and had different antibacterial and antibiofilm activities with different concentrations. Moreover, previous studies had investigated the antibacterial and antibiofilm activities in separate protocols and didn’t investigate the real-time or dual effect of silver nanoparticles on both planktonic and sessile cells within single protocol. This study aimed to synthesize silver nanoparticles through three methods and analyzing the simultaneous antibacterial and antibiofilm activities against planktonic and sessile bacterial cells. Three methods were applied to analyze silver nanoparticles and used to investigate the dual effect against bacterial biomass and biofilm formation. The results showed that synthesized silver nanoparticles cause significant inhibition to bacterial cell biomass and bacterial biofilm formation when compared with controls at low concentration. Significant Higher antibiofilm activity than antibacterial activity was observed at very low concentration (0.0125 µg/ ml). The antibacterial and antibiofilm activity do not differ according to the type of bacteria. Whereas, the antibacterial effect differs significantly according to the methods of silver nanoparticles synthesis.

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