scholarly journals Acceleration of Skin Wound-Healing Reactions by Autologous Micrograft Tissue Suspension

Medicina ◽  
2020 ◽  
Vol 56 (7) ◽  
pp. 321 ◽  
Author(s):  
Shiro Jimi ◽  
Satoshi Takagi ◽  
Francesco De Francesco ◽  
Motoyasu Miyazaki ◽  
Arman Saparov
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Smooth Muscle Actin ◽  
Skin Grafting ◽  
Skin Wound ◽  
Control Group ◽  
Distribution Analysis ◽  
Skin Wound Healing ◽  
Tissue Samples ◽  
Tissue Reactions

Background and objectives: Skin grafting is a method usually used in reconstructive surgery to accelerate skin regeneration. This method results frequently in unexpected scar formations. We previously showed that cutaneous wound-healing in normal mice is accelerated by a micrograft (MG) technique. Presently, clinical trials have been performed utilizing this technology; however, the driving mechanisms behind the beneficial effects of this approach remain unclear. In the present study, we focused on five major tissue reactions in wound-healing, namely, regeneration, migration, granulation, neovascularization and contraction. Methods: Morphometrical analysis was performed using tissue samples from the dorsal wounds of mice. Granulation tissue formation, neovascularization and epithelial healing were examined. Results: The wound area correlated well with granulation sizes and neovascularization densities in the granulation tissue. Vascular distribution analysis in the granulation tissue indicated that neovessels extended and reached the subepidermal area in the MG group but was only halfway developed in the control group. Moreover, epithelialization with regeneration and migration was augmented by MG. Myofibroblast is a known machinery for wound contraction that uses α-smooth muscle actin filaments. Their distribution in the granulation tissue was primarily found beneath the regenerated epithelium and was significantly progressed in the MG group. Conclusions: These findings indicated that MG accelerated a series of wound-healing reactions and could be useful for treating intractable wounds in clinical situations.

scholarly journals Impact of acacia arabica topical gel on skin wound healing: An experimental study

Pharmacia ◽  
2022 ◽  
Vol 69 (1) ◽  
pp. 77-83
Author(s):  
Eman Abdullah ◽  
Sara Taha ◽  
Noor Sulaiman ◽  
Muna Ahmed
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Healing Process ◽  
Skin Wound ◽  
Control Group ◽  
Wound Healing Process ◽  
Study Group ◽  
Skin Wound Healing ◽  
Topical Gel ◽  
Acacia Arabica

Aim of the study: To estimate the influence of Acacia Arabica gel abstract on skin wound healing. Material and method: 28male adult rabbits were included, 2 incisions of 1cm length were made on the skin of the rabbit’s back, one is treated by acacia gel while another left for normal healing, histopathological examination was done at 24h, 3, 7 and 14 days intervals. Results: The inflammatory reactions showed no significant results for both the control group and the study group during the study intervals. Granulation tissue in the control group was absent after 24h but increased to become profound after 7 days then become moderate, while the study group showed scanty granulation tissue from the first day and increased through study intervals. Re-epithelialization mean scores in the study group were higher than control one. Conclusions: Acacia arabica has the efficacy of hastening the wound healing process.

Cold Plasma Treatment Promotes Full-thickness Healing of Skin Wounds in Murine Models

2021 ◽  
pp. 153473462110021
Author(s):  
Joon M. Jung ◽  
Hae K. Yoon ◽  
Chang J. Jung ◽  
Soo Y. Jo ◽  
Sang G. Hwang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Plasma Treatment ◽  
Cold Plasma ◽  
Smooth Muscle Actin ◽  
Treated Group ◽  
Control Group ◽  
Alpha Smooth Muscle Actin ◽  
Skin Wound Healing

Cold plasma can be beneficial for promoting skin wound healing and has a high potential of being effectively used in treating various wounds. Our aim was to verify the effect of cold plasma in accelerating wound healing and investigate its underlying mechanism in vitro and in vivo. For the in vivo experiments, 2 full-thickness dermal wounds were created in each mouse (n = 30). While one wound was exposed to 2 daily plasma treatments for 3 min, the other wound served as a control. The wounds were evaluated by imaging and histological analyses at 4, 7, and 11 days post the wound infliction process. Immunohistochemical studies were also performed at the same time points. In vitro proliferation and scratch assay using HaCaT keratinocytes and fibroblasts were performed. The expression levels of wound healing–related genes were analyzed by real-time polymerase chain reaction and western blot analysis. On day 7, the wound healing rates were 53.94% and 63.58% for the control group and the plasma-treated group, respectively. On day 11, these rates were 76.05% and 93.44% for the control and plasma-treated groups, respectively, and the difference between them was significant ( P = .039). Histological analysis demonstrated that plasma treatment promotes the formation of epidermal keratin and granular layers. Immunohistochemical studies also revealed that collagen 1, collagen 3, and alpha-smooth muscle actin appeared more abundantly in the plasma-treated group than in the control group. In vitro, the proliferation of keratinocytes was promoted by plasma exposure. Scratch assay showed that fibroblast exposure to plasma increased their migration. The expression levels of collagen 1, collagen 3, and alpha-smooth muscle actin were elevated upon plasma treatment. In conclusion, cold plasma can accelerate skin wound healing and is well tolerated.

scholarly journals EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS

2020 ◽  
Vol 33 (1) ◽  
Author(s):  
Letícia Fuganti CAMPOS ◽  
Eliane TAGLIARI ◽  
Thais Andrade Costa CASAGRANDE ◽  
Lúcia de NORONHA ◽  
Antônio Carlos L. CAMPOS ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Type I Collagen ◽  
Chronic Wounds ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Type I ◽  
Collagen Deposition ◽  
Skin Wound Healing

ABSTRACT Background: Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions. Aim: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats. Methods: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E. Results: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p<0.001) and more neovessels (p=0.001). Conclusions: Perioperative supplementation of probiotics stimulates skin wound healing in diabetic rats, possibly due to attenuation of the inflammatory response and increased neovascularization and type I collagen deposition.

scholarly journals The Effects of Hydrogen-Rich Water on Skin Wound Healing in Dogs

2021 ◽  
Author(s):  
Dong-Dong Qi ◽  
Ting Wang ◽  
Muhammad Abid Hayat ◽  
Tao Liu ◽  
JianTao Zhang
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Healing Time ◽  
Skin Wound ◽  
Control Group ◽  
Sod Activity ◽  
Skin Wound Healing ◽  
Histopathological Results ◽  
Wound Healing Time ◽  
Skin Homogenate

Abstract Background: Hydrogen-rich water (HRW) has been shown to be one of underlying therapeutic strategies regarding wound treatments. This study explored the effects of drinking HRW on skin wound healing in dogs. Eight circular wounds were analyzed in each dog. The experimental group was treated with HRW twice daily, while the control group was provided with distilled water (DW). The wound tissues of dogs examined histopathologically. The fibroblasts, inflammatory cell infiltration, the average number of new blood vessels, and the level of malondialdehyde (MDA) and superoxide dismutase (SOD) activity in the skin homogenate of the wound was measured using the corresponding kits. The expression of Nrf-2, HO-1, NQO-1, VEGF, and PDGF were measured by real-time fluorescence quantitative method. Results: We observed that HRW wound had significant average healing rate and faster average healing time. Histopathological results showed that the average number of blood vessels and the average thickness of epidermis were significantly different from the DW group. The MDA levels were higher in the DW group than in the HRW group but the SOD levels were higher in the HRW group than in the DW group. The results of qRT-PCR showed that the expression of each gene was significantly different between the two groups. Conclusions: The results showed that HRW treatment could promote skin wound healing in dogs, accelerate wound epithelization, reduce inflammatory reaction, stimulate the expression of cytokines related to wound healing, and shorten wound healing time.

scholarly journals A Novel Multilayer Composite Membrane for Wound Healing in Mice Skin Defect Model

Polymers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 573 ◽  
Author(s):  
Yuyu Qiu ◽  
Qingqing Wang ◽  
Yajun Chen ◽  
Shufang Xia ◽  
Wei Huang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Healing Process ◽  
Animal Experiments ◽  
Skin Wound ◽  
Control Group ◽  
Skin Defect ◽  
Multilayer Composite ◽  
Defect Model ◽  
Skin Wound Healing

To develop a wound dressing material that conforms to the healing process, we prepared a multilayer composite (MC) membrane consisting of an antibacterial layer (ABL), a reinforcement layer (RFL), and a healing promotion layer (HPL). Biocompatible zein/ethyl cellulose (zein/EC) electrospun nanofibrous membranes with in situ loaded antibacterial photosensitizer protoporphyrin (PPIX) and healing promotion material vaccarin (Vac) were, respectively, chosen as the ABL on the surface and the HPL on the bottom, between which nonwoven incorporated bacterial cellulose (BC/PETN) as the HPL was intercalated to enhance the mechanical property. Photodynamic antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa was confirmed by the enlarged inhibition zones; meanwhile, satisfactory biocompatibility of the HPL was verified by scanning electronic microscopy (SEM) of L929 cells cultured on its surface. The potential effects on wound healing in a mice skin defect model of the MC membranes were also evaluated. The animal experiments demonstrated that the wound healing rate in the MC group was significantly increased compared with that in the control group (p < 0.05). Histopathological observation revealed an alleviated inflammatory response, accompanied with vascular proliferation in the MC group. The MC membranes significantly promoted wound healing by creating an antibacterial environment and promoting angiogenesis. Taken together, this MC membrane may act as a promising wound dressing for skin wound healing.

scholarly journals CICATRIZAÇÃO CUTÂNEA POR SEGUNDA INTENÇÃO EM EQÜINOS TRATADOS COM VEDAPROFENO

2004 ◽  
Vol 9 (1) ◽  
Author(s):  
C.A. HUSSNI ◽  
C.G. GIANINI ◽  
A.L.G. ALVES ◽  
J.L.M. NICOLETTI ◽  
A. THOMASSIAN ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Lesions ◽  
Skin Wound ◽  
Post Treatment ◽  
Control Group ◽  
Control Groups ◽  
Skin Wound Healing ◽  
Total Period ◽  
Histopathological Studies ◽  
Macroscopic Examination

Com o objetivo de avaliar-se possíveis efeitos do vedaprofeno sobre a cicatrização de pele em eqüinos, realizaram-se lesões circulares de pele padronizadas com “punch” de 2 cm, nas regiões lombares direita e esquerda de 14 eqüinos. Estes dois últimos foram divididos em dois grupos, os quais receberam tratamento local diário com líquido de Dakin. Sete animais foram tratados com 2 mg/kg de vedaprofeno (grupo vedaprofeno) via oral, duas vezes ao dia, durante dez dias e, sete animais do grupo controle não receberam tratamento. Em todos os cavalos, as lesões do lado direito foram avaliadas macroscopicamente e por planimetria a cada seis dias e, as feridas do lado esquerdo foram analisadas por material para histopatologia no sexto e 15º dias de tratamento. Comparativamente, as lesões dos animais tratados com vedaprofeno mostraram-se secas e sem edema perilesional e as feridas controle apresentaram maior retração centrípeta. O tempo total de cicatrização não diferiu, variando entre 28 e 39 dias (média 32,86) nos animais do grupo vedaprofeno e entre 26 e 39 dias nos animais do grupo controle (média 31,71). Nos exames histopatológicos ocorreu diferença entre os grupos quanto à epitelização presente observada com maior freqüência no grupo controle. O vedaprofeno diminuiu a retração e epitelização da ferida com redução da fase exsudativa e do edema, entretanto, sem interferir no tempo total de cicatrização. Second intention skin wound healing in equines under vedaprofen treatment Abstract The aim of this study was to evaluate the effects vedaprofen of on equine skin wound healing. Circular skin lesions using a 2 cm punch were induced on right and left lumbar regions in 14 equines. The animals were divided into two groups and the wounds received daily topic treatment with Dakin solution. Seven horses (vedaprofen group) were treated with vedaprofen (2mg/kg PO BID) for 10 days, and seven (control group) were not treated. All animals had right side wounds evaluated by macroscopic examination and planimetry each 6 days and the left side wounds were submitted to histopathological studies at the 6th and 15th post-treatment days. The wounds of vedaprofene group showed drier lesions without edema when compared to the control groups wounds, which displayed more centripetal retraction.The total healing period was similar in both groups, with 28 and 39 days (average 32.86) in the vedaprofen group and 26 and 39 days (average 31.71) in the control group. The epithelial recovery was evaluated by histopathological examinations and was more often seen in the control group. The vedaprofen diminished the retraction and epithelial recovery of the wound, but did not interfere in the total period of healing with reduction of exsudative phase and edema.

scholarly journals Mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar rats

2016 ◽  
Vol 46 (10) ◽  
pp. 1824-1829
Author(s):  
Betânia Souza Monteiro ◽  
Roberta Delessa Faria ◽  
Alessandra Rodrigues Coelho Zanella ◽  
Emanuelle Pimentel Cruz ◽  
Noely Pianca Godoi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Normal Saline ◽  
Skin Wound ◽  
Control Group ◽  
Intravenous Route ◽  
Single Application ◽  
Skin Wound Healing ◽  
Dexamethasone Group ◽  
Surgical Wounds ◽  
Immunosuppressed Patients

ABSTRACT: To evaluate the therapeutic contribution of MSC intravenous infusion to surgical wound healing in dexamethasone-immunosuppressed rats, thirty-five rats were randomly divided into 2 groups: in the Control Group (CG), five rats received normal saline as 0.2ml subcutaneous (SC) injections every 24 hours, for 30 consecutive days and, in the Dexamethasone Group (DG), 30 rats were given 0.2mL subcutaneous dexamethasone (0.1mg kg-1) every 24 hours, for 30 consecutive days. After 30 days, all rats underwent surgery to create an experimental skin wound. The 30 animals of the DG group were divided into two equal groups, which received different treatments: the dexamethasone group (DG) received a single application of 0.5ml normal saline, via the intravenous route (IV), 48 hours after wound creation; and the Mesenchymal Stem Cells Dexamethasone group (MSCDG) received MSC transplantation at a concentration of 9x106 cells in a single IV application, 48 hours after wound creation. The surgical wounds of CG rats closed on average 14.75 days after creation and DG rats had wounds closed within 22 days; whereas, the surgical wounds of MSCDG rats were closed in 14 days. MSC infusion in dexamethasone-immunosuppressed patients contributed positively to epithelial healing in less time.

scholarly journals Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments

2008 ◽  
Vol 53 (No. 12) ◽  
pp. 652-659 ◽  
Author(s):  
P. Gal ◽  
Kilik ◽  
R ◽  
M. Mokry ◽  
B. Vidinsky ◽  
...  
Keyword(s):  
Wound Healing ◽  
Quantitative Methods ◽  
Healing Process ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Wound Healing Process ◽  
Polymorphonuclear Leucocytes ◽  
Skin Wound Healing ◽  
Simple Method

The use of a simple and reproducible model is inevitable for objective statement of the effects of external factors on wound healing. Hence, present study was conducted to establish an excisional model of skin wound healing in corticosteroid treated, and streptozotocine induced diabetic rats as well as to standardized the semi-quantitative and quantitative evaluation of selected parameters. Round full thickness skin wounds were performed on the back of male Sprague-Dawley rats. Animals were sacrificed two, six, and fourteen days after surgery. Sections were stained with hematoxylin-eosin and van Gieson. Both semi-quantitative (wound reepithelization; presence of: inflammatory cells, fibroblasts, new wessels, and collagen) and quantitative methods (polymorphonuclear leucocytes/tissue macrophages ratio, percentage of re-epithelization, area of the granulation tissue) were used to evaluate the histological changes during wound healing. As compared to the control group the wound healing process of both experimental groups was decelerated. Interestingly, wound reepithelization and angiogenesis were significantly inhibited only in the steroid rats while epithelization was accelerated in diabetic rats. In conclusion, when compared to primary sutured wound healing it can be concluded that the excisional model is more appropriate for histological assessment of the effect of various factors on wound healing. In addition, administration of corticosteroids represents simple and inexpensive model of a complex skin wound healing impairment.

scholarly journals Local and Remote Effects of Mesenchymal Stem Cell Administration on Skin Wound Regeneration

2021 ◽  
Vol 28 (3) ◽  
pp. 355-373
Author(s):  
Ekaterina Silina ◽  
Victor Stupin ◽  
Konstantin Koreyba ◽  
Sergey Bolevich ◽  
Yulia Suzdaltseva ◽  
...  
Keyword(s):  
Wound Healing ◽  
Direct Injection ◽  
Medical Problem ◽  
Local Effect ◽  
Skin Wound ◽  
Regeneration Process ◽  
Control Group ◽  
Skin Wound Healing ◽  
Inflammatory Processes ◽  
Series Of Experiments

Wound healing is an important medical problem. We evaluated the efficacy of locally administered mesenchymal stem cells (MSCs) isolated from human umbilical cords on the dynamics of skin wound healing. The study was conducted on the backs of Wistar rats, where two square wounds were created by removing all layers of the skin. Four groups were studied in two series of experiments: (1) a Control_NaCl group (the wounds were injected with 0.9% NaCl solution) and a Control_0 group (intact wounds on the opposite side of the same rat’s back); (2) an MSC group (injected MSCs, local effect) and a Control_sc group (intact wounds on the opposite side of the back, remote MSC effect). The area and temperature of the wounds and the microcirculation of the wound edges were measured. Histological and morphometric studies were performed on days 3 and 7 after the wounds were created. The results showed that the injection trauma (Control_NaCl) slowed the regeneration process. In both MSC groups (unlike in either control group), we observed no increase in the area of the wounds; in addition, we observed inhibition of the inflammatory process and improved wound regeneration on days 1–3 in the remote group and days 1–5 in the local (injected) group. The MSC and Control_sc groups demonstrated improved microcirculation and suppression of leukocyte infiltration on day 3. On day 7, all the studied parameters of the wounds of the Control_0 group were the same as those of the wounds that received cell therapy, although in contrast to the results of the Control_ NaCl group, fibroblast proliferation was greater in the MSC and Control_sc groups. The dynamics of the size of the wounds were comparable for both local and remote application of MSCs. Thus, even a one-time application of MSCs was effective during the first 3–5 days after injury due to anti-inflammatory processes, which improved the regeneration process. Remote application of MSC, as opposed to direct injection, is advisable, especially in the case of multiple wounds, since the results were indistinguishable between the groups and injection trauma was shown to slow healing.

scholarly journals Features of skin wound healing in rats with experimental chronic kidney disease

2021 ◽  
Vol 12 (4) ◽  
pp. 594-598
Author(s):  
S. B. Pavlov ◽  
O. B. Litvinova ◽  
N. M. Babenko
Keyword(s):  
Chronic Kidney Disease ◽  
Wound Healing ◽  
Growth Factors ◽  
Kidney Disease ◽  
Wound Repair ◽  
Histological Analysis ◽  
Kidney Diseases ◽  
Skin Wound ◽  
Control Group ◽  
Skin Wound Healing

Chronic kidney disease negatively affects the morphofunctional state of all organs due to hemodynamic and metabolic disorders. Changes in the content of cytokines observed in kidney diseases, which regulate the processes of inflammation and tissue repair, can complicate the course of the wound process. This research aimed to study disorders in the process of skin wound repair due to changes in the dynamics of production of interleukins IL-1β, IL-6, IL-10, IL-4, growth factors bFGF and VEGF in animals with experimental chronic kidney disease. The levels of interleukins and growth factors were determined on the 7th, 14th and 28th days after surgical modeling of wounds in the blood of rats with experimental chronic kidney disease and animals of the control group. To assess the dynamics and quality of wound healing, a semi-quantitative histological analysis was performed. The study showed an increase in the content of pro-inflammatory interleukins in the group of sick rats: on the 7th day the level of IL-1β was 1.19 times higher, and IL-6 – 1.55 times, on the 14th day the level of IL-1β was 1.37 times in comparison with the control group. The maximum increase in the concentration of anti-inflammatory interleukins was noted on the 28th day: IL-4 was 2.10 times higher, IL-10 – 1.39 times higher than in the control group. The content of bFGF and VEGF in animals of the control group reached its maximum on the 7th day, and in animals with chronic kidney disease – on the 15th day after surgery. Semi-quantitative histological analysis showed a decrease in indicators in the group of sick animals: the number of fibroblasts and collagen deposition – on the 7th day, reepithelialization – on the 28th day. A persistent increase in the number of polymorphonuclear leukocytes was also noted at all periods of the experiment: by 1.38, 1.99, and 9.82 times – on the 7th, 14th, and 28th days, respectively. The study showed that the dynamics of the production of interleukins and growth factors were impaired in rats with chronic kidney disease. In the process of damage regeneration in sick animals, pro-inflammatory mechanisms prevailed with the involvement of a large number of immunocompetent cells, as a result, skin wounds took longer to heal.

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