scholarly journals Isolation and Characterization of Effective Bacteria That Reduce Ammonia Emission from Livestock Manure

2021 ◽  
Vol 10 (1) ◽  
pp. 77
Author(s):  
Sun-Il Kim ◽  
Wan Heo ◽  
So-Jung Lee ◽  
Young-Jun Kim
Keyword(s):  
Effective Strain ◽  
Evaluation Criteria ◽  
Ammonia Excretion ◽  
Airborne Particulate Matter ◽  
Ammonia Emission ◽  
Chemical Components ◽  
Livestock Manure ◽  
Ammonia Gas ◽  
Treatment Groups ◽  
Isolation And Characterization

Ammonia from livestock manure reacts with chemical components discharged from various emission sources to produce airborne particulate matter. This study aimed to investigate a novel effective microbial agent to suppress ammonia gas emitted from manure. Both isolated L12I and 12III strains, identified as Pediococcus acidilactici (PA), were selected for their superior activity in assays performed with the evaluation criteria such as acid production, ammonia decomposition, and urease inhibition, which are key factors influencing ammonia excretion. The survivability of PA strains was confirmed by an increase in DNA abundance in the manure. PA strains lowered the pH of manure and suppressed the growth of hyper-ammonia-producing bacteria (HAB) possessing urease activity. The L12I and 12III treatment groups showed 23.58% and 38.00% emission reductions, respectively. Especially, the 12III strain was proven to be the more effective strain for reducing ammonia gas emission, with the best ability to reduce pH and inhibit HAB. The strains could have an additive effect in improving the manure quality as a nitrogen fertilizer by preserving the total nitrogen and urea content. These results suggest that PA strains can be used as unprecedented microbial agents to improve manure-derived environmental pollution and improve fertilizer quality.

Isolation and HPLC Determination of the Chemical Components of Gentianella acuta (Michx.) Hulten

2018 ◽  
Vol 15 (1) ◽  
pp. 21-33
Author(s):  
Ying Wei ◽  
Yongqiao Liu ◽  
Yifan Hele ◽  
Weiwei Sun ◽  
Yang Wang ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
High Speed ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Gradient Elution ◽  
Chemical Components ◽  
Isolation And Characterization ◽  
Major Chemical ◽  
Main Components ◽  
First Time

Background: Gentianella acuta (Michx.) Hulten is an important type of medicinal plant found in several Chinese provinces. It has been widely used in folk medicine to treat various illnesses. However, there is not enough detailed information about the chemical constituents of this plant or methods for their content determination. Objective: The focus of this work is the isolation and characterization of the major chemical constituents of Gentianella acuta, and developing an analytical method for their determination. Methods: The components of Gentianella acuta were isolated using (1) ethanol extraction and adsorption on macroporous resin. (2) and ethyl acetate extraction and high speed countercurrent chromatography. A HPLC-DAD method was developed using a C18 column and water-acetonitrile as the mobile phase. Based on compound polarities, both isocratic and gradient elution methods were developed. Results: A total of 29 compounds were isolated from this plant, of which 17 compounds were isolated from this genus for the first time. The main components in this plant were found to be xanthones. The HPLC-DAD method was developed and validated for their determination, and found to show good sensitivity and reliability. Conclusion: The results of this work add to the limited body of work available on this important medicinal plant. The findings will be useful for further investigation and development of Gentianella acuta for its valuable medicinal properties.

scholarly journals REDUCTION OF AMMONIA EMISSIONS FROM THE COMPOSTING OF SLAUGHTERHOUSE SOLID WASTE USING ZEOLITE

2020 ◽  
Vol 3 (2) ◽  
pp. 164
Author(s):  
Rhenny Ratnawati ◽  
Sugito Sugito
Keyword(s):  
Solid Waste ◽  
High Capacity ◽  
Raw Material ◽  
Ammonia Emission ◽  
Ammonia Emissions ◽  
Impact Study ◽  
Aerobic Composting ◽  
Ammonia Gas ◽  
Gas Emissions ◽  
Composting Process

The process of aerobic composting the slaughterhouse (SH) solid waste generate ammonia emissions. Aim: The objective of this research to study the ability of the adsorbent to use zeolite to reduce ammonia gas emissions during the composting process of SH solid waste. Methodology and Results: Reduction of ammonia emission is conducted during the aerobic composting process which is 50 days. The raw material composition of the composting process used was 100% rumen contents, 60% rumen contents: 40% straw, 50% rumen contents: 50% straw, and 40% rumen contents: 60% straw. Zeolite used in the form of granular size 100 mesh. The result of the research showed that the level of release of ammonia gas emissions during the composting process could be reduced by zeolite. Conclusion, significance, and impact study: The efficiency of reducing ammonia gas emissions using zeolite adsorbents in the composting process of SH solid waste ranges from 98.09 - 99.40% on average. Zeolite is an adsorbent that has high adsorption power because it has many pores and has a high ion exchange high capacity and serves as an absorbent cation that can cause environmental pollution.

Extraction, Isolation, Characterization and Antioxidant Potential of Pure Compounds from Adonsonia digitata Leaf extract

2019 ◽  
Author(s):  
Isaac John Umaru ◽  
Hauwa A. Umaru ◽  
Fasihuddin Badruddin Ahmed
Keyword(s):  
Leaf Extract ◽  
Bioactive Compound ◽  
Biological Properties ◽  
Antioxidant Potential ◽  
Chemical Components ◽  
Plant Parts ◽  
Chromatography Analysis ◽  
Isolation And Characterization ◽  
Pure Compounds ◽  
Anti Oxidant

Abstract Introduction Adonsonia digitata L. (Malvaceae) commonly known as Baobab is a medicinal and nutritional plant. The plant parts are used to treat various ailments such as diarrhoea, malaria and microbial infections. It is reported that it is an excellent anti-oxidant due to its vitamin C content. Baobab has numerous biological properties including antimicrobial, antiviral, anti-oxidant and anti-inflammatory activities amongst others. Objective: The study involves extraction, Isolation, Characterisation of phytochemicals and evaluation of antioxidant potential of the pure compounds. Methods: The dried leaf powder was subjected to rotary evaporator to obtain crude extract which was subjected to isolation using chromatography analysis and elucidation using NMR and FTIR. Antioxidant (IC 50 ) potential was determined using 2,2-diphenyl-1- picryl-hydrazyl-hydrate (DPPH). Result: Phytochemical investigation of the Baobab leaf extract through isolation and characterization of a bioactive compound was observed. The leaf extract yielded three compound characterised as D-Limonene, Thunbergol and (z)-2-Methyl-7-octadecene. Conclusion: The analysis of the chemical component reported identified compounds from Baobab leaf as; D-Limonene; ( 1 ) Thunbergol ( 2 ) and Cis-2-Methyl-7-octadecene ( 3 ) with significant antioxidant potential. The chemical components were identified for the first time.

Isolation and Characterization of Flavonoids from Black Glutinous Rice (Oryza Sativa L. Var Glutinosa)

2019 ◽  
Vol 20 (2) ◽  
pp. 6-16
Author(s):  
Sri Benti Etika ◽  
Iryani Iryani
Keyword(s):  
Oryza Sativa ◽  
Melting Point ◽  
Oryza Sativa L ◽  
Wave Number ◽  
Chemical Components ◽  
Isolation And Characterization ◽  
Glutinous Rice ◽  
Ft Ir ◽  
Powder Analysis

This study aims to isolate and characterize the isolated flavonoid compounds from glutinous rice (Oryza Sativa L. Var Glutinosa). The method that used was maserasi with methanol solvent, multilevel fractionation with n-hexane and ethyl acetate. The separation of chemical components using column chromatography and tested its purity by melting point test and TLC. Characterization of isolated flavonoids using color reagents (H2SO4, NaOH, Mg-HCl), KKt-2A, UV-Vis and FT-IR. The powder (amorphous) flavonoid isolation results have a melting point of 236.5-237.8 oC. Flavonoid powder added H2SO4 produces a yellow color, with NaOH producing a violet color, and Mg-HCl produces a red color. KKt-2A with a BAA developer has Rf 0.49 and 15% acetic acid having Rf 0.09. The UV-Vis spectrum shows maximum absorption at 532 nm wavelength. The results of powder analysis (amorphous) flavonoid using FT-IR showed absorption at wave number 3.330,40 cm-1, 2838,96 cm-1, 1,656,23 cm-1, 1,421,85 cm-1, 1,112,25 cm-1, 1,019,27 cm-1 and 697,76 cm-1. According to those data it can be concluded that flavonoids that isolated have the form of powder (amorphous) which belong to anthocyanin group that have o-diOH on ring B and have a conjugated double bond.Keywords- Black Sticky Rice, Flavonoid, Fractionation, FT-IR, Maserasi, UV-Vis.

Evaluation of full-scale biofilter with rockwool mixture treating ammonia gas from livestock manure composting

2009 ◽  
Vol 100 (4) ◽  
pp. 1568-1572 ◽  
Author(s):  
Tomoko Yasuda ◽  
Kazutaka Kuroda ◽  
Yasuyuki Fukumoto ◽  
Dai Hanajima ◽  
Kazuyoshi Suzuki
Keyword(s):  
Full Scale ◽  
Livestock Manure ◽  
Ammonia Gas

scholarly journals Marine, freshwater and aerially acclimated mangrove rivulus (Kryptolebias marmoratus) use different strategies for cutaneous ammonia excretion

2013 ◽  
Vol 304 (8) ◽  
pp. R599-R612 ◽  
Author(s):  
Christopher A. Cooper ◽  
Jonathan M. Wilson ◽  
Patricia A. Wright
Keyword(s):  
Immunofluorescence Microscopy ◽  
Ammonia Excretion ◽  
Teleost Fishes ◽  
Ammonia Gas ◽  
Ammonia Transport ◽  
Paracellular Pathways ◽  
Kryptolebias Marmoratus ◽  
Rh Glycoproteins ◽  
Mangrove Rivulus ◽  
Ammonia Flux

Rhesus (Rh) glycoproteins are ammonia gas (NH3) channels known to be involved in ammonia transport in animals. Because of the different osmoregulatory and ionoregulatory challenges faced by teleost fishes in marine and freshwater (FW) environments, we hypothesized that ammonia excretion strategies would differ between environments. Also, we hypothesized that cutaneous NH3 volatilization in air-acclimated fish is facilitated by base secretion. To test these hypotheses, we used the skin of the euryhaline amphibious mangrove rivulus ( Kryptolebias marmoratus). The skin excretes ammonia and expresses Rh glycoproteins. Serosal-to-mucosal cutaneous ammonia flux was saturable (0–16 mmol/l ammonia, Km of 6.42 mmol/l). In FW, ammonia excretion increased in response to low mucosal pH but decreased with pharmacological inhibition of Na+/H+ exchangers (NHE) and H+ ATPase. Conversely, in brackish water (BW), lowering the mucosal pH significantly decreased ammonia excretion. Inhibitors of NHE also decreased ammonia excretion in BW fish. Immunofluorescence microscopy demonstrated that both the Rh isoform, Rhcg1, and NHE3 proteins colocalized in Na+/K+ ATPase expressing mitochondrion-rich cells in the gills, kidney, and skin. We propose that the mechanisms of cutaneous ammonia excretion in FW K. marmoratus are consistent with the model for branchial ammonia excretion in FW teleost fish. NH4+ excretion appeared to play a stronger role in BW. NH4+ excretion in BW may be facilitated by apical NHE and/or diffuse through paracellular pathways. In aerially acclimated fish, inhibition of NHE and H+ ATPase, but not the Cl−/HCO3− exchanger, significantly affected cutaneous surface pH, suggesting that direct base excretion is not critical for NH3 volatilization. Overall, K. marmoratus use different strategies for excreting ammonia in three different environments, FW, BW, and air, and Rh glycoproteins and NHE are integral to all.

scholarly journals In vivo Anthelminthic Activity of Ethanolic Leaf Extract of Senna italica on Rats with Hymenolepis diminuta Infection

2020 ◽  
pp. 18-27
Author(s):  
Aliyu Mahmuda ◽  
Mansur Sani ◽  
Tukur Adamu ◽  
Aminu Sanda ◽  
Lauwali Galadima Gobir
Keyword(s):  
Leaf Extract ◽  
Animal Experiments ◽  
Hymenolepis Diminuta ◽  
In Vivo Studies ◽  
Chemical Components ◽  
Infection Model ◽  
Phytochemical Analysis ◽  
Treatment Groups ◽  
Ethanolic Leaf Extract

In spite of the effectiveness of most anthelmintic agents in use, there is still need to identify more due to their unwanted side effects. Hence, the need to develop more that can be safe for all, cheap and available even in our localities. In the light of the current need for the use of traditional plants in the treatment of parasitic diseases, we have assessed the anthelmintic effect of Senna italica and aimed to investigate the therapeutic activity on Hymenolepis diminuta infection. We described in vivo studies evaluating the anthelmintic effects of the leaf extract at different concentrations in a murine infection model. Phytochemical analysis of the plant extract has shown the chemical components available in the compound. There was a significant (p<0.05) reduction in mean egg/gram (EPG) in one of the treatment groups which was observed to be similar to that of the treatment using conventional anthelmintic agent (Albendazole). A fluctuating but insignificant faecal worm-egg count was observed in the other experimental groups. The results obtained suggest a likelihood of its future use as an anthelmintic agent. Our next plan is to establish animal experiments with different types of helminth infection in order to have the full anthelmintic coverage by the agent, hopefully, before it will be validated for use as a human therapeutic agent.

scholarly journals Chemical Composition and Source Apportionment of Wintertime Airborne PM2.5 in Changchun, Northeastern China

2021 ◽  
Vol 18 (8) ◽  
pp. 4354
Author(s):  
Shichun Zhang ◽  
Daniel Q. Tong ◽  
Mo Dan ◽  
Xiaobing Pang ◽  
Weiwei Chen ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Glass Melting ◽  
Chemical Species ◽  
Airborne Particulate Matter ◽  
Northeastern China ◽  
Mitigation Measures ◽  
Chemical Components ◽  
Industrial Facilities ◽  
Arithmetic Average ◽  
Inorganic Aerosols

This study presents field observations and laboratory analyses of wintertime airborne particulate matter (PM2.5) and its chemical components in the Changchun metropolitan area, the geographical center of northeastern China. Twenty-four hour PM2.5 filter samples were collected from 23 December 2011 to 31 January 2012 at four sites in the types of traffic, residential, campus, and a near-city rural village, respectively. Daily PM2.5 concentrations ranged from 49 to 466 µg m−3, with an arithmetic average of 143 µg m−3. Laboratory analyses showed that among all measured chemical species, mineral dust contributed the largest proportion (20.7%) to the total PM2.5 mass, followed by secondary inorganic aerosols (SIA, including SO42−, NO3− and NH4+), which constituted 18.8% of PM2.5 mass. Another notable feature of PM2.5 chemical composition was high halogen (Cl− and F−) loadings at all sites, which was likely due to emissions from coal combustion, plastic manufacturing, and glass melting. Among the four sampling sites, the suburban site exhibited the highest PM2.5 levels and extremely high Cl− and F− loadings due to residential wood burning and nearby industrial facilities lacking effective emission controls. Our results report one of the earliest observations of PM2.5 composition in this region, providing a baseline of aerosol profiles of aerosol before PM2.5 was routinely measured by environmental protection agencies in China, which could be useful for assessing long-term trends of air quality and effectiveness of mitigation measures.

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