scholarly journals Interactions between Magnaporthiopsis maydis and Macrophomina phaseolina, the Causes of Wilt Diseases in Maize and Cotton

2020 ◽  
Vol 8 (2) ◽  
pp. 249 ◽  
Author(s):  
Ofir Degani ◽  
Shlomit Dor ◽  
Dekel Abraham ◽  
Roni Cohen
Keyword(s):  
Fungal Pathogens ◽  
Water Regime ◽  
Growth Parameters ◽  
Harmful Effect ◽  
Soil Fungus ◽  
Macrophomina Phaseolina ◽  
Maturity Stage ◽  
Double Infection ◽  
Yield Production ◽  
Series Of Experiments

Fungal pathogens are a significant threat to crops worldwide. The soil fungus, Magnaporthiopsis maydis, severely affects sensitive maize hybrids by causing the rapid wilting of plants at the maturity stage. Similarly, the soil fungus, Macrophomina phaseolina, develops in a variety of host plants, which leads to rot and plant mortality. The presence of both pathogens together in diseased cotton plants in Israel suggests possible interactions between them. Here, these relationships were tested in a series of experiments accompanied by real-time PCR tracking in maize and cotton. Despite the fact that neither of the pathogens was superior in a growth plate confrontation assay, their co-inoculum had a significant influence under field conditions. In maize sprouts and fully matured plants, infection by both pathogens (compared to inoculation with each of them alone) led to lesser amounts of M. maydis DNA but to increased amounts of M. phaseolina DNA levels. These results were obtained under a restricted water regime, while optimal water irrigation led to less pronounced differences. In water-stressed cotton sprouts, infection with both pathogens led to an increase in DNA amounts of each of the pathogens. Whereas the M. maydis DNA levels in the double infection remain high at the end of the season, a reduction in the amount of M. phaseolina DNA was observed. The double infection caused an increase in growth parameters in maize and cotton and decreased levels of dehydration in maize plants accompanied by an increase in yield production. Dehydration symptoms were minor in cotton under an optimal water supply. However, under a restricted water regime, the double infection abolished the harmful effect of M. phaseolina on the plants’ development and yield. These findings are the first report of interactions between these two pathogens in maize and cotton, and they encourage expanding the study to additional plant hosts and examining the potential involvement of other pathogens.

scholarly journals Rhizosphere-enriched microbes as a pool to design synthetic communities for reproducible beneficial outputs

2019 ◽  
Author(s):  
Maria-Dimitra Tsolakidou ◽  
Ioannis A Stringlis ◽  
Natalia Fanega-Sleziak ◽  
Stella Papageorgiou ◽  
Antria Tsalakou ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Growth Parameters ◽  
Tomato Plants ◽  
Beneficial Effects ◽  
Negative Effect ◽  
Tomato Growth ◽  
In Vitro Antifungal Activity

Abstract Composts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, for in vitro antifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters in Arabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genus Bacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth of Arabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect on Arabidopsis and tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect on Arabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn't affect Arabidopsis growth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

Effects of Different Nitrogen Fertilizers and Humic Acid On Wheat Crop Irrigated With Different Water Regime Using 15N

2021 ◽  
Vol 50 (2) ◽  
pp. 261-267
Author(s):  
Ahmed A Moursy ◽  
MM Ismail
Keyword(s):  
Dry Matter ◽  
Water Regime ◽  
Soil Depth ◽  
N Uptake ◽  
Nitrogen Fertilizers ◽  
Wheat Crop ◽  
Water Requirements ◽  
Applied Water ◽  
Yield Production ◽  
Uptake Data

A field experiment was conducted to observe effects of water requirements and different fertilizers on wheat crop’s yield, production and N uptake. Data showed that dry matter yield of wheat grain was higher with Hu + AS (5.82 mt/ha) compared with applied water 100% ETC. Concerning the rate of water regime, the best significant grain yield of wheat was obtained with 100% ETc (4.23 mt/ha). Nitrogen derived from fertilizer Ndff% with 50% ETC of water was 28.41 and 27.28% for grain and straw, respectively. At 100% ETC of water the Ndff% was 30.16 and 27.75% for grain and straw, respectively. Nitrogen utilized by grains and straw was more efficient under treatment Hu + AS combined with 50% Etc, 100% Etc recording 15.6 and 32.23%, respectively. At 50% ETC of water requirements for wheat crop, higher N remained in 0 - 15, 15 - 30 and 30 - 45 cm soil depth were nearly closed to each other compared with the treatment made at 100% ETC of water requirements. Bangladesh J. Bot. 50(2): 261-267, 2021 (June)

scholarly journals Side effects of triazoles on treated crops

2021 ◽  
Author(s):  
Michal Jakl ◽  
Sanja Ćavar Zeljković ◽  
Ishak Kovač ◽  
Kateřina Bělonožníková ◽  
Jana Jaklová Dytrtová
Keyword(s):  
Oxidative Stress ◽  
Side Effects ◽  
Fungal Pathogens ◽  
Crop Production ◽  
Growth Parameters ◽  
Target Species ◽  
Fruit Peel ◽  
Biochemical Processes ◽  
Basic Plant ◽  
Cherry Tomatoes

Triazolic fungicides are widely applied in crop production to protect plants against fungal pathogens. However, they may influence the biochemical processes in plants and other non-target species. This paper is aimed at the effect of triazoles (namely tebuconazole, cyproconazole, and penconazole) single/mixed applications on the phenolics production in tomato (Solanum lycopersicum L.) fruit peel, amount of chlorophyll a and b in tomato leaves as well as on basic plant growth parameters. For this purpose, cherry tomatoes were planted in the pot experiment and foliarly-treated weekly, with the same total triazoles dose of 3.52 μmol per plant (in mixtures of 1.71 or 1.17 μmol of each in two or three components, respectively). The treatments increased the weight of fruits in the 1 harvest about 43%, however, this effect was not observed in the next harvest. Increased oxidative stress in the triazoles presence was observed, based on the elevated production of antioxidant phenolics in the 1 harvest. Most alarming is the decrease of the weight of thin stems and foliage and the concentration of chlorophyll a (b) in leaves in all triazoles-treated variants. The non-target impacts on plant biochemical processes (related to the phenolics or chlorophylls production and functionality) were confirmed.

scholarly journals Rhizosphere-enriched microbes as a pool to design synthetic communities for reproducible beneficial outputs

2018 ◽  
Author(s):  
Maria-Dimitra Tsolakidou ◽  
Ioannis A. Stringlis ◽  
Natalia Fanega-Sleziak ◽  
Stella Papageorgiou ◽  
Antria Tsalakou ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Growth Parameters ◽  
Tomato Plants ◽  
Beneficial Effects ◽  
Negative Effect ◽  
Tomato Growth ◽  
Selection Of

AbstractComposts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, forin vitroantifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters inArabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genusBacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth ofArabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect onArabidopsisand tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect onArabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn’t affectArabidopsisgrowth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

scholarly journals Application of Mycorrhizae for Controlling Root Diseases of Sesame

2011 ◽  
Vol 51 (4) ◽  
pp. 355-361 ◽  
Author(s):  
El-Sayed Ziedan ◽  
Ibrahim Elewa ◽  
Mostafa Mostafa ◽  
Ahmed Sahab
Keyword(s):  
Root System ◽  
Seed Yield ◽  
Root Rot ◽  
Fungal Pathogens ◽  
Arbuscular Mycorrhizae ◽  
Trichoderma Viride ◽  
Macrophomina Phaseolina ◽  
Morphological Characters ◽  
Va Mycorrhizae ◽  
Root Diseases

Application of Mycorrhizae for Controlling Root Diseases of Sesame Vesicular arbuscular mycorrhizae fungi (VAM) was evaluated as a biotic agent for controlling wilt and root-rot diseases of sesame caused by Fusarium oxysporum f. sp. sesami (Zap.) Cast and Macrophomina phaseolina (Moubl) Ashby pathogens can infect sesame plant at any growth stage causing considerable losses of seed yield. Spores of VA mycorrhizae fungi (Glomus spp.) were collected from the soil around the root systems of sesame plants then propagated on roots of Suddan grass (Sorghum vulgare var. sudanese). Under green house and field conditions, two hundreds sporocarps of Glomus spp. were added as a soil drench beside the sesame plant. Glomus spp. (VA mycorrhizae) significantly reduced wilt and root-rot incidence of sesame plants. Lums spp. (VA mycorrhizae) also significantly increased plant morphological characters such as plant height, number of branches and number of pods for each plant. Application of Glomus spp. to protect sesame plants by colonizing the root system, significantly reduced colonization of fungal pathogens in sesame rhizosphere as well as pathogenic activity of fungal pathogens increased lignin contents in the sesame root system were also observed. Furthermore, mycorrhizae treatment provided selective bacterial stimulation for colonization on sesame rhizosphere. These bacteria belonging the Bacillus group showed highly antagonistic potential to fungal pathogens. Application of mycorrhizae together with other biocontrol agent such as Trichoderma viride or Bacillus subtilis significantly effected than individual treatments for controlling these diseases incidences and increasing morphological characters and seed yield of sesame.

Effects of postveraison water regime on the phenolic composition of grapes and wines of cv. Agiorgitiko (Vitis vinifera L.)

OENO One ◽  
2013 ◽  
Vol 47 (2) ◽  
pp. 115 ◽  
Author(s):  
Stefanos Koundouras ◽  
Ioannis Kanakis ◽  
Efrosyni Drossou ◽  
Stamatina Kallithraka ◽  
Yorgos Kotseridis
Keyword(s):  
Vitis Vinifera ◽  
Water Deficit ◽  
Water Regime ◽  
Growth Parameters ◽  
Significant Loss ◽  
Climatic Conditions ◽  
Anthocyanin Content ◽  
Phenolic Composition ◽  
Stem Water Potential ◽  
Irrigation Amount

<p style="text-align: justify;"><strong>Aims</strong>: Vine water deficit is widely accepted as a powerful means to control grape and wine attributes. However, quality improvement is often achieved at the expense of a reduction in yield, especially when water deficit conditions are applied during the preveraison period. The aim of the present work was to test an irrigation regime based on manipulating water availability from veraison to harvest, as a means to control berry and wine composition with minimum effect on reproductive growth parameters.</p><p style="text-align: justify;"><strong>Methods and results</strong>: A field trial was conducted during two consecutive years (2007-2008) in Nemea, Southern Greece. Three irrigation treatments were applied on seven-year-old, vertical shoot positioned and spur pruned Agiorgitiko vines (<em>Vitis vinifera</em> L.), from veraison through harvest : irrigation at 70 % of crop evapotranspiration (ET<sub>c</sub>) (I70), irrigation at 30 % of ET<sub>c</sub> (I30) and non irrigated (NI). Irrigation amount produced significant differences in postveraison midday stem water potential pattern, especially during the drier year 2008. Yield was increased by irrigation in 2008, whereas berry growth was unaffected in both seasons. Berries of NI vines achieved higher total skin anthocyanin content in 2007, although individual anthocyanin levels were not affected by water regime. Irrigation effect on skin tannins was inconsistent but seed tannins were higher in I70 vines, with increased levels of catechin and epicatechin monomers. Among wine attributes, tannin concentration, but not anthocyanin, was mostly responsive to water deficit-induced changes in berry phenolic composition. The wines made from I70 grapes had a higher tannin content than those made from NI grapes.</p><p style="text-align: justify;"><strong>Conclusions</strong>: The results presented show that postveraison water regime had a significant effect on skin anthocyanins and, more markedly, on seed tannins, without altering berry growth parameters. Especially for seed tannins, this effect appears to predominate over variations in climatic conditions between years.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: This trial suggests that Agiorgitiko vines grown on the loamy soils of Nemea perform better under non irrigated conditions during the postveraison period since rainfed vines had improved phenolic composition (higher colour with lower contribution of seed tannins) without significant loss in productivity.</p>

scholarly journals Bio-stimulation by seed priming with Bacillus subtilis for suppressing seed-borne fungal pathogens of vegetables in Bangladesh

2017 ◽  
Vol 14 (2) ◽  
pp. 177-184
Author(s):  
FH Tumpa ◽  
A Sultana ◽  
MZ Alam ◽  
MAR Khokon
Keyword(s):  
Bacillus Subtilis ◽  
Fungal Pathogens ◽  
Culture Method ◽  
Seed Priming ◽  
Macrophomina Phaseolina ◽  
Fungal Species ◽  
Dual Culture ◽  
Control Seed ◽  
Rhizopus Sp ◽  
Wax Gourd

Aqueous formulation of rhizopheric beneficial bacteria viz. Bacillus subtilis, an exotic strain collected from Russia was assayed for its performance to suppress the growth of various seed-borne fungi of vegetable. The performance of the bio-agent was appraised based on growth suppression by dual culture method and reduction of seed-borne fungi in the bio-agent treated seeds. Ten seed samples comprise of summer and winter vegetables were examined collected from different local farmers. A total of fourteen fungal species belongs to twelve genera viz. Fusarium moniliforme, Botrytis cinerea, Aspergillus flavus, F. oxysporum, Botryodiplodia theobromae, Macrophomina phaseolina, A.niger, Cercospora sp., Phoma exigua, Rhizopus sp., Colletotrichum sp., Phytophthora sp., Penicillium sp., and Curvularia sp. were recorded from different untreated seeds. Vegetables seeds were treated in a concentration (10-3) of aqueous formulation of B. subtilis for two hours followed by air drying for 30 minutes. In general, the incidences of all fungal species of all kinds of vegetables seeds were reduced. But, selectively the best performances were observed in tomato, brinjal, cucumber, wax gourd and okra where most of the fungal species were completely suppressed by seed treatment with Bacillus subtilis. The growth of seed-borne fungi was inhibited highest in wax gourd (100%). The growth inhibition was also at satisfactory level in cucumber (95%), tomato (95%), brinjal (92%) and okra (86%) seeds. The findings of this research indicate the possibility of utilizing B. subtilis as seed treating agents instead of chemical fungicides to control seed-borne diseases of vegetables.J. Bangladesh Agril. Univ. 14(2): 177-184, December 2016

scholarly journals Genetic and biochemical studies on some Egyptian wheat genotypes under drought stress

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Samy A. A. Heiba ◽  
Samira A. Osman ◽  
Sara E. I. Eldessouky ◽  
Atef A. A. Haiba ◽  
Rania T. Ali
Keyword(s):  
High Performance ◽  
Water Regime ◽  
Pollen Viability ◽  
Protein Profile ◽  
Pollen Grains ◽  
Water Shortage ◽  
Betaine Aldehyde Dehydrogenase ◽  
Wheat Genotypes ◽  
Yield Production ◽  
Stress Susceptibility

Abstract Background Strategic crops need to be screened from time to time to select the most tolerant and stable genotypes incompatible with environmental conditions. This research experiments were carried out to screen thirteen Egyptian wheat genotypes under water regime stress to select the most tolerant genotypes to drought conditions in two successive seasons. The studied genotypes were cultivated in rows, and the irrigation duration period between any two successive irrigations was elongated to double period to supply these genotypes with only 50% of the water needed in well watering conditions. This evaluation concerned on some yield production indexes such as spike weight, spike length, spike number, grains number/spike the 100 grains weight and stress susceptibility index and the cytogenetic evaluation index of the pollen grains viability on two successive seasons (2017–2018 and 2018–2019). This screening also carried out some biochemical and molecular analysis on the wheat genotypes at the seedling stage by the end of 2018 (the first season of water regime) targeting total protein profile, proline amino acid accumulation and Betaine aldehyde dehydrogenase-1 enzyme gene expression via semiquantitative RT-PCR. Results Sids 12 genotype recorded the highest SW, SL and SN. Considering the number of grains/plant and the number of spikes/plant are the principal yield components, and 100 grains weight is the principal yield quality, Sids 12 also declared its tolerance to water regime on regarding the plant productivity to grains’ number/plant and scored the lowest SSI. Pollen viability and protein profile were also affected as a response to the water regime. Proline concentration in fresh leaves emphasizes the tolerance of Sids 12, Misr 2 and Gemmieza 9 genotypes to the water deficit. Conclusion Among the examined genotypes, Sids 12, Misr 2, Shandaweel1 and Gemmieza 9 and line 20 are recommended to be cultivated in water shortage conditions as they show high performance under water regime conditions.

scholarly journals Efficacy of Fungicides against Soil Borne and Grapevine Pathogens under In vitro Conditions

2021 ◽  
Vol 12 (5) ◽  
pp. 523-531
Author(s):  
Anjali Singh ◽  
◽  
Ratna U. Thosar ◽  
Vijayshree Chavan ◽  
Sujoy Saha ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Xanthomonas Campestris ◽  
Sclerotium Rolfsii ◽  
Inhibition Zone ◽  
Macrophomina Phaseolina ◽  
Research Centre ◽  
Grape Vine ◽  
Rhizoctonia Bataticola ◽  
Wide Range

In vitro bio-efficacy of the novel copper (NC101 and NCP102) and phosphonate (PN103 and PMN104) based fungicides against various soil borne and grape vine pathogens was evaluated at ICAR-National Research Centre for Grapes, Pune, Maharashtra, India during February - April 2021. The fungicides were screened against five bacterial pathogens viz. Xanthomonas campestris pv. citri, X. campestris pv. campestris, X. campestris pv. punicae, X. campestris pv. viticola and X. oryzae pv. oryzae and 10 fungal pathogens viz. Rhizoctonia bataticola, Sclerotium rolfsii, Cladosporium sp, Alternaria alternata, Colletotrichum gloeosporioides, Penicillium notatum, Magnaporthe oryzae, Fusarium oxysporium, Macrophomina phaseolina (Soyabean isolate) and Macrophomina phaseolina (Jute isolate) at different concentrations. Results indicated that among all the tested fungicides viz. NC101, NCP102, PN103 and PMN104, phosphonate based fungicides (PN103 and PMN104) were highly effective against bacterial isolates with zone of inhibition ranging between 8.75 - 31.12 mm in which X. campestris pv. viticola was found to express least inhibition zone. In case of pathogenic fungal isolates, similar trend was observed, most of the isolates showed cent percent inhibition at higher concentration of PN103 and PMN104. However Sclerotium rolfsii showed least or no inhibition when tested at different concentrations of fungicides. The chemicals exhibited wide range of inhibition and it was found to increase steadily with increase in concentrations of the test fungicides.

scholarly journals Gamma Radiation (60Co) Exposure and Application of Antagonists for the Suppression of Root Rot Diseases in Sunflower (Helianthus annus L.) and Mung Bean (Vigna radiata (L.) R. Wilczek)

Our Nature ◽  
1970 ◽  
Vol 8 (1) ◽  
pp. 26-33 ◽  
Author(s):  
S. Dawar ◽  
M.J. Zaki ◽  
N. Ikram ◽  
M. Tariq
Keyword(s):  
Gamma Radiation ◽  
Gamma Rays ◽  
Root Rot ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Growth Parameters ◽  
Macrophomina Phaseolina ◽  
Root Weight ◽  
Complete Suppression ◽  
Helianthus Annus

An experiment was carried out for the control of root rot fungi such as Fusarium spp., Macrophomina phaseolina (Tassi) Goid., and Rhizoctonia solani Kühn in Sunflower (Helianthus annus L.) and Mung bean (Vigna radiata (L.) R. Wilczek). Seeds were treated with gamma radiation (60Co) for 0, 2, 8 and 16 minutes intervals and soil was drenched with different antagonists such as Trichoderma harzianum Rifai, Pseudomonas aeruginosa (Schroeter) Migula and Rhizobium meliloti Dangeard. All the antagonists significantly increased germination percentage, shoot length, root length, shoot weight, root weight, leaf area and showed complete suppression of M. phaseolina and R. solani observed in mung bean and sunflower. In both crops, mostly growth parameters increased as the exposure of gamma rays increased whereas in some cases it decreased. P. aeruginosa and R. meliloti were observed to be satisfactory biocontrol agents followed by T. harzianum whereas all exposure time of irradiation of gamma rays (60Co) to seeds of mung bean and sunflower were found to be best.DOI: 10.3126/on.v8i1.4309

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