scholarly journals 16S and 23S rRNA Gene Mutation Independent Multidrug Resistance of Non-Tuberculous Mycobacteria Isolated from South Korean Soil

2020 ◽  
Vol 8 (8) ◽  
pp. 1114
Author(s):  
Hyun-Eui Park ◽  
Suji Kim ◽  
Soojin Shim ◽  
Hong-Tae Park ◽  
Woo Bin Park ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Multidrug Resistance ◽  
South Korea ◽  
Urban Areas ◽  
23S Rrna ◽  
Rrna Gene ◽  
Aminoglycoside Resistance ◽  
Resistance Profile ◽  
South Korean ◽  
Antibiotic Resistance Profile

Non-tuberculous mycobacteria (NTM) are ubiquitous microorganisms that have the potential to cause disease in both humans and animals. Recently, NTM infections have rapidly increased in South Korea, especially in urbanized areas. However, the distribution of species and the antibiotic resistance profile of NTM in environmental sources have not yet been investigated. Therefore, we analyzed the distribution of species and the antibiotic resistance profile of NTM in soil within urban areas of South Korea. A total of 132 isolates of NTM were isolated from soil samples from 1 municipal animal shelter and 4 urban area parks. Among the 132 isolates, 105 isolates were identified as slowly growing mycobacteria (SGM) and 27 isolates as rapidly growing mycobacteria (RGM) based on the sequences of the rpoB and hsp65 genes. The antibiotic resistance patterns of NTM isolates differed from species to species. Additionally, a mutation in the rrs gene found in this study was not associated with aminoglycoside resistance. In conclusion, our results showed that NTM isolates from South Korean soil exhibit multidrug resistance to streptomycin, amikacin, azithromycin, ethambutol, isoniazid, and imipenem. These results suggest that NTM may pose a public threat.

scholarly journals Antibiotic resistance profile of Klebsiella pneumoniae strains isolated in an infectious diseases clinic

2021 ◽  
Vol 24 (1) ◽  
pp. 32-37
Author(s):  
Lucian Giubelan ◽  
◽  
Livia Dragonu ◽  
Andreea Cristina Stoian ◽  
Florentina Dumitrescu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Infectious Diseases ◽  
Urban Areas ◽  
Demographic Data ◽  
Resistance Index ◽  
Multidrug Resistant ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Polymyxin E

Objective. To establish the resistance profile of Klebsiella pneumoniae (KP) strains isolated in the Craiova Infectious Diseases Clinic. Material and method. Retrospective study (January 2017-December 2018); KPs were identified using the automated Vitek 2 system, which subsequently established their susceptibility to antimicrobials (usual testing for 17 antibiotics, extended testing for another 9); for each strain the multiple antibiotic resistance index (MAR) was calculated (limits: 0-1); the information was entered into an Excel database. 45 strains were tested in 2017 and 290 in 2018. The percentage of MDR strains was calculated based on internationally accepted definitions. Results. 335 strains were identified, the vast majority isolated by sputum culture (192 strains - 57.31%). Demographic data: 330 strains (99%) were isolated in adult subjects, 190 (57%) in male patients, 185 (55%) in patients living in urban areas. The overall value of MAR was 0.37 (compared to 0.32 for all strains of isolated Gram-negative germs). Over 80% of KP strains were susceptible to Amikacin or Polymyxin E; susceptibilities between 60 and 79% were observed for Meropenem, Gentamicin, Tobramycin, Ciprofloxacin, Ertapenem, Trimethoprim-Sulfamethoxazole, Levofloxacin, Cefepima, and between 40 and 59% for Ceftriaxone, Cefoxitin, Ceftazidime, Minocycline and Im. Less than 39% of strains are susceptible to Ampicillin (± Sulbactam), Piperacillin (± Tazobactam), Ticarcillin (± Clavulanic acid), Aztreonam, Pefloxacin, Cefazolin, Nitrofurantoin. Resistance to the main classes of antibiotics shows lower values than national and european data for 3rd generation cephalosporins, fluoroquinolones and aminoglycosides, but a much higher percentage of resistance to carbapenems. Over 68% of isolates are multidrug-resistant (MDR); over 60% of the strains come from sources where there is a significant contact with antimicrobials. Conclusions. Over 80% of the isolated strains are sensitive to Amikacin or Polymyxin E; a percentage of over 29% of the strains demonstrates resistance to the carbapenem class in 2018; over 68% of isolates are multidrug-resistant; over 60% of the strains come from sources where there is significant contact with antimicrobials.

scholarly journals Distribution of Antibiotic MICs for Helicobacter pylori Strains over a 16-Year Period in Patients from Seoul, South Korea

2004 ◽  
Vol 48 (12) ◽  
pp. 4843-4847 ◽  
Author(s):  
Jung Mogg Kim ◽  
Joo Sung Kim ◽  
Hyun Chae Jung ◽  
Nayoung Kim ◽  
Young-Jeon Kim ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
South Korea ◽  
Clinical Problem ◽  
23S Rrna ◽  
Rrna Gene ◽  
Primary Resistance ◽  
The Past ◽  
23S Rrna Gene ◽  
H Pylori

ABSTRACT Recently, the development of antibiotic resistance emerged as a significant clinical problem in the eradication of Helicobacter pylori. We investigated the MICs of antibiotics for 135 H. pylori isolates from adults in Seoul, South Korea, over the past 16 years. The MICs of amoxicillin, clarithromycin, metronidazole, tetracycline, azithromycin, and ciprofloxacin increased from 1987 to 2003. Rates of primary resistance to clarithromycin increased from 2.8% in 1994 to 13.8% in 2003. The A2144G mutation was frequently observed in the 23S rRNA gene in clarithromycin-resistant isolates. The increase in resistance to clarithromycin seems to result in a decrease in eradication efficacy for H. pylori. These results suggest that the MICs of several antibiotics for H. pylori have increased over the past 16 years in Seoul.

scholarly journals Comparison of Antibiotic Resistance Profile of Escherichia coli between Pristine and Human-Impacted Sites in a River

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 575
Author(s):  
Emi Nishimura ◽  
Masateru Nishiyama ◽  
Kei Nukazawa ◽  
Yoshihiro Suzuki
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Resistant Bacteria ◽  
Downstream Site ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Upstream Site ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Significant Difference

Information on the actual existence of antibiotic-resistant bacteria in rivers where sewage, urban wastewater, and livestock wastewater do not load is essential to prevent the spread of antibiotic-resistant bacteria in water environments. This study compared the antibiotic resistance profile of Escherichia coli upstream and downstream of human habitation. The survey was conducted in the summer, winter, and spring seasons. Resistance to one or more antibiotics at upstream and downstream sites was on average 18% and 20%, respectively, and no significant difference was observed between the survey sites. The resistance rates at the upstream site (total of 98 isolated strains) to each antibiotic were cefazolin 17%, tetracycline 12%, and ampicillin 8%, in descending order. Conversely, for the downstream site (total of 89 isolated strains), the rates were ampicillin 16%, cefazolin 16%, and tetracycline 1% in descending order. The resistance rate of tetracycline in the downstream site was significantly lower than that of the upstream site. Furthermore, phylogenetic analysis revealed that many strains showed different resistance profiles even in the same cluster of the Pulsed-Field Gel Electrophoresis (PFGE) pattern. Moreover, the resistance profiles differed in the same cluster of the upstream and the downstream sites. In flowing from the upstream to the downstream site, it is plausible that E. coli transmitted or lacked the antibiotic resistance gene.

scholarly journals ANTIBIOTIC RESISTANCE PROFILING OF DAIRY WASTEWATER DEGRADING NATIVE EFFICIENT MICROBIAL ISOLATES

2017 ◽  
Vol 10 (7) ◽  
pp. 145
Author(s):  
Anupama Bhardwaj ◽  
Jagtar Singh ◽  
Sonia Chaman ◽  
Amit Joshi
Keyword(s):  
Antibiotic Resistance ◽  
Oxygen Demand ◽  
Dairy Wastewater ◽  
Organic Load ◽  
Diffusion Method ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Surrounding Environment ◽  
Before And After ◽  
Microbial Isolates

Objective: The objective of this study is to make sure biotreatment process used for treatment of dairy wastewater (DWW) is safe for human and its surrounding environment; microbes were evaluated for their antibiotic resistance profile against commonly prescribed antibiotics. Methods: Microbes were isolated using spread plating and streaking method and used to treat DWW. Reduction in organic load in DWW was determined by comparing physicochemical parameters (PCP) of DWW before and after treatment process. After selection of efficient microbial isolates, they were evaluated for their antibiotic resistance profile using antibiotic disc diffusion method. Results: In this work, 53 microbes were isolated from DWW, and these microbial isolates were screened for DWW degradation capacity by analyzing PCP. Four microbial isolates E3, E5, E11 (bacterial isolates) and F5 (fungal isolate) showed highest reduction in chemical oxygen demand (COD), biological oxygen demand (BOD), and dissolved oxygen (DO) were selected for profound degradation of DWW under optimized conditions. Efficient four microbial isolates individually performed better under anaerobic conditions by showing maximum reduction 84%, 75%, and 77% in COD, BOD, and DO, respectively. After 72 hrs of antibiotic susceptibility testing, E3 strain had shown 100%, E5 90%, E11 70%, and F5 80% susceptibility to antibiotics. Conclusion: The present study concluded that four microbial isolates had the potential of reducing the organic load of DWW along with lessor or negligible adverse effect on human or its surrounding environment and they appear to be most promising strains for treatment of DWW. 

scholarly journals Identification and Antibiotic Resistance Profile of Uropathogenic Bacteria from Sexually Active Women with Bacterial Vaginosis

2021 ◽  
Vol 09 (11) ◽  
pp. 52-67
Author(s):  
Chinedum Taahie Oparaugo ◽  
Bamidele Abiodun Iwalokun ◽  
Adesegun Abiola Adesesan ◽  
Idowu Olaide Edu-Muyideen ◽  
Abimbola Modupe Adedeji ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Bacterial Vaginosis ◽  
Sexually Active ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Uropathogenic Bacteria

scholarly journals A global perspective of antibiotic-resistant Listeria monocytogenes prevalence in assorted ready to eat foods: A systematic review

2021 ◽  
pp. 2219-2229
Author(s):  
Prudence Mpundu ◽  
Allan Rabson Mbewe ◽  
John Bwalya Muma ◽  
Wizaso Mwasinga ◽  
Nawa Mukumbuta ◽  
...  
Keyword(s):  
Systematic Review ◽  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Meta Analysis ◽  
Global Perspective ◽  
Valuable Insight ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Pooled Prevalence ◽  
Insight Into

Background and Aim: Listeria monocytogenes in ready-to-eat (RTE) foods remains consistently under-reported globally. Nevertheless, several independent studies conducted to investigate have elucidated the prevalence and antibiotic resistance profiles of L. monocytogenes in RTE-associated foods and their antibiotic resistance profiles. Given the rapid increase in consumption of RTE foods of both animal and plant origin, it is imperative to know the prevalence deductive data focusing on how much of L. monocytogenes is present in RTE foods, which is critical for food safety managers and retailers to assess the possible risk posed to end-users. In addition, valuable insight and another angle to the depth of the problem, we conducted a systematic review and meta-analysis to synthesize available data regarding the prevalence of L. monocytogenes in RTE foods and antibiotic resistance profiles. Materials and Methods: We conducted a meta-analysis study of L. monocytogenes and antibiotic resistance to clinically relevant antibiotics to determine the extent of L. monocytogenes contamination in RTE foods and antibiotic resistance profiles. The primary search terms, also known as keywords used, were restricted to peer-reviewed and review articles, and databases, including Google Scholars, Science-Direct, and Scopus, were searched. The inclusion of articles meeting eligibility criteria published between 2010 and 2020 after title, abstract, and full article screening. Data analysis was performed at multiple stages using quantitative meta-analysis reviews. Results: L. monocytogenes pooled proportion/prevalence was highest in chicken products determined at (22%) followed by various but uncategorized RTE foods at 21%. Regarding antibiotic resistance, profiling's highest pooled prevalence resistance was observed in penicillin at 80% resistance, followed by cephalosporin at 47%. Conclusion: Within its limitations, this study has attempted to provide insight into the pooled proportion/prevalence of L. monocytogenes in RTE foods and the antibiotic resistance profile at the global level. Determining the proportion/ prevalence of L. monocytogenes in RTE foods across the globe and antibiotic resistance profile is essential for providing quality food and reducing public health problems due to unsuccessful treatment of foodborne illness. This study provides insight into the pooled prevalence of L. monocytogenes in RTE foods and the antibiotic resistance profile. The results of this study partly endeavored to help appropriate authorities strengthen their preventive measures on specific RTE foods that are most likely to be contaminated with L. monocytogenes and antibiotic resistance profiles.

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