Composition and Antimicrobial Activities of Lippia multiflora Moldenke, Mentha x piperita L. and Ocimum basilicum L. Essential Oils and Their Major Monoterpene Alcohols Alone and in Combination

L’alternariose de la tomate est une maladie cryptogamique dévastatrice dans les zones maraichères du Burkina Faso. Tous les organes de la plante (tiges, feuilles, fruits) sont attaqués, entrainant une baisse importante du rendement et une dépréciation de la qualité de la production. Dans la présente étude, quatre huiles essentielles de plantes aromatiques locales (Cymbopogon schoenanthus, Lippia multiflora, Ocimum americanum, et Ocimum basilicum) ont été testées, pour évaluer leurs propriétés d'inhibition sur la croissance radiale du mycélium d’un isolat pathogène de Alternaria sp. Le test in vitro des doses de 100%, 50%, 10%, 5% et 1% a montré que toutes ces huiles essentielles possèdent une activité antifongique sur ledit isolat. Les taux d’inhibition des huiles essentielles sur la croissance du champignon étudié vont de 25,96% à 100%. La meilleure inhibition a été enregistrée avec l’huile essentielle de Cymbopogon schoenanthus, de Ocimum basilicum et de Lippia multiflora pour les concentrations supérieures à 1%. Il ressort donc de cette étude que trois huiles essentielles, stoppent la croissance mycélienne de l’isolat pour des doses allant de 5% à 100%. Ces huiles (Cymbopogon schoenanthus, Ocimum basilicum et Lippia multiflora) pourraient être utilisées pour la mise au point de phytopesticides afin d'atténuer l’utilisation intensive et hasardeuse des pesticides.Mots clés: Alternariose, activité antifongique, plante aromatique, tomate. English Title: Effects of four essential oils on radial mycelial growth of an isolate of Alternaria sp. in Burkina Faso Early blight of tomato is a devastating cryptogamic disease in the vegetable gardens in Burkina Faso. All plant’s organs (stems, leaves, fruits) are attacked causing a significant decrease in the yield and a depreciation of the quality of the production in the field. In the present study, four essential oils of aromatic plants (Cymbopogon schoenanthus, Lippia multiflora, Ocimum americanum, and Ocimum basilicum) were tested to evaluate the inhibition properties of these oils on the radial growth of the mycelium of an isolated pathogen of Alternaria sp. In vitro tests at doses of 100%, 50%, 10%, 5% and 1% showed that all these essential oils tested have antifungal activity on said strain. The inhibition rates of essential oils on the growth of the fungus studied range from 25.96% to 100%. The best inhibition was recorded with the essential oil of Cymbopogon schoenanthus, Ocimum basilicum and Lippia multiflora for applied concentrations more than 1%. It therefore emerges from this study that three essential oils stop the mycelial growth of the isolate for doses ranging from 5% to 100%. These oils (Cymbopogon schoenanthus, Ocimum basilicum and Lippia multiflora) could be used for the development of phytopesticides in order to reduce the intensive and hazardous use of pesticides.Keywords: Early blight, antifungal activity, aromatic plant, tomato.

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Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations

Food Chemistry ◽

10.1016/j.foodchem.2007.12.010 ◽

2008 ◽

Vol 108 (3) ◽

pp. 986-995 ◽

Cited By ~ 440

Author(s):

Abdullah Ijaz Hussain ◽

Farooq Anwar ◽

Syed Tufail Hussain Sherazi ◽

Roman Przybylski

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Seasonal Variations ◽

Antimicrobial Activities ◽

Ocimum Basilicum ◽

Antioxidant And Antimicrobial Activities

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Potential Acetylcholinesterase, Lipase, α-Glucosidase, and α-Amylase Inhibitory Activity, as well as Antimicrobial Activities, of Essential Oil from Lettuce Leaf Basil (Ocimum basilicum L.) Elicited with Jasmonic Acid

Applied Sciences ◽

10.3390/app10124315 ◽

2020 ◽

Vol 10 (12) ◽

pp. 4315 ◽

Cited By ~ 1

Author(s):

Urszula Złotek ◽

Kamila Rybczyńska-Tkaczyk ◽

Monika Michalak-Majewska ◽

Małgorzata Sikora ◽

Anna Jakubczyk

Keyword(s):

Jasmonic Acid ◽

Essential Oils ◽

Inhibitory Activity ◽

Biological Activities ◽

Control Sample ◽

Antimicrobial Activities ◽

Ocimum Basilicum ◽

Gram Negative Bacteria ◽

Gram Positive Bacteria ◽

Significant Difference

The aim of this study was to investigate the influence of the elicitation with jasmonic acid on the biological activities of essential oils (EOs) from lettuce leaf basil (Ocimum basilicum L.). Specifically, 0.01 µM jasmonic acid (JA1), 1 µM jasmonic acid (JA2), and 100 µM jasmonic acid (JA3) were used as elicitors. The results indicated that the elicitation increased the acetylcholinesterase, lipase, and α-amylase inhibitory activity of essential oils. A significant difference in α-glucosidase inhibition was noted only for the JA3 extract (IC50 = 0.81 µL/mL), as this activity was lower than in the control sample without elicitation (IC50 = 0.68 µL/mL). The studied basil EOs exhibited similar activity against Staphylococcus aureus (Gram-positive bacteria) and Escherichia coli (Gram-negative bacteria). Based on the value of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentrations (MBC), the best antimicrobial activity was observed for JA2 and JA3.

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Chemical composition and antimicrobial activities of two Mentha species essential oils

10.1055/s-0039-3400014 ◽

2019 ◽

Author(s):

A Filip ◽

I Boz ◽

S Dunca ◽

G-A Ștefan ◽

M-M Zamfirache

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Antimicrobial Activities

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Foeniculum vulgare Essential Oils: Chemical Composition, Antioxidant and Antimicrobial Activities

Natural Product Communications ◽

10.1177/1934578x1000500231 ◽

2010 ◽

Vol 5 (2) ◽

pp. 1934578X1000500 ◽

Cited By ~ 15

Author(s):

Maria Graça Miguel ◽

Cláudia Cruz ◽

Leonor Faleiro ◽

Mariana T. F. Simões ◽

Ana Cristina Figueiredo ◽

...

Keyword(s):

Essential Oils ◽

Radical Scavenging ◽

Antimicrobial Activities ◽

Point Of View ◽

Diffusion Method ◽

Foeniculum Vulgare ◽

Aerial Parts ◽

Scavenging Capacity ◽

Dpph Method ◽

Main Components

The essential oils from Foeniculum vulgare commercial aerial parts and fruits were isolated by hydrodistillation, with different distillation times (30 min, 1 h, 2 h and 3 h), and analyzed by GC and GC-MS. The antioxidant ability was estimated using four distinct methods. Antibacterial activity was determined by the agar diffusion method. Remarkable differences, and worrying from the quality and safety point of view, were detected in the essential oils. trans-Anethole (31-36%), α-pinene (14-20%) and limonene (11-13%) were the main components of the essentials oil isolated from F. vulgare dried aerial parts, whereas methyl chavicol (= estragole) (79-88%) was dominant in the fruit oils. With the DPPH method the plant oils showed better antioxidant activity than the fruits oils. With the TBARS method and at higher concentrations, fennel essential oils showed a pro-oxidant activity. None of the oils showed a hydroxyl radical scavenging capacity >50%, but they showed an ability to inhibit 5-lipoxygenase. The essential oils showed a very low antimicrobial activity. In general, the essential oils isolated during 2 h were as effective, from the biological activity point of view, as those isolated during 3 h.

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Synergistic Antimicrobial Activities of Combinations of Vanillin and Essential Oils of Cinnamon Bark, Cinnamon Leaves and Cloves

Foods ◽

10.3390/foods10061406 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1406

Author(s):

Rita Cava-Roda ◽

Amaury Taboada-Rodríguez ◽

Antonio López-Gómez ◽

Ginés Benito Martínez-Hernández ◽

Fulgencio Marín-Iniesta

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Food Packaging ◽

Antimicrobial Effect ◽

Antimicrobial Activities ◽

Inhibitory Effects ◽

Cinnamon Bark ◽

E Coli ◽

Pure Compounds ◽

Main Component

Plant bioactive compounds have antimicrobial and antioxidant activities that allow them to be used as a substitute for synthetic chemical additives in both food and food packaging. To improve its sensory and bactericidal effects, its use in the form of effective combinations has emerged as an interesting possibility in the food industry. In this study, the antimicrobial activities of essential oils (EOs) of cinnamon bark, cinnamon leaves, and clove and the pure compounds vanillin, eugenol, and cinnamaldehyde were investigated individually and in combination against Listeria monocytogenes and Escherichia coli O157:H7. The possible interactions of combinations of pure compounds and EOs were performed by the two-dimensional checkerboard assay and isobologram methods. Vanillin exhibited the lowest antimicrobial activity (MIC of 3002 ppm against L. monocytogenes and 2795 ppm against E. coli O157:H7), while clove and cinnamon bark EOs exhibited the highest antimicrobial activity (402–404 against L. monocytogenes and 778–721 against E. coli O157:H7). For L. monocytogenes, pure compound eugenol, the main component of cinnamon leaves and clove, showed lower antimicrobial activity than EOs, which was attributed to the influence of the minor components of the EOs. The same was observed with cinnamaldehyde, the main component of cinnamon bark EO. The combinations of vanillin/clove EO and vanillin/cinnamon bark EO showed the most synergistic antimicrobial effect. The combination of the EOs of cinnamon bark/clove and cinnamon bark/cinnamon leaves showed additive effect against L. monocytogenes but indifferent effect against E. coli O157:H7. For L. monocytogenes, the best inhibitory effects were achieved by cinnamon bark EO (85 ppm)/vanillin (910 ppm) and clove EO (121 ppm)/vanillin (691 ppm) combinations. For E. coli, the inhibitory effects of clove EO (104 ppm)/vanillin (1006 ppm) and cinnamon leaves EO (118 ppm)/vanillin (979 ppm) combinations were noteworthy. Some of the tested combinations increased the antimicrobial effect and would allow the effective doses to be reduced, thereby offering possible new applications for food and active food packaging.

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The Use of Essential Oils from Thyme, Sage and Peppermint against Colletotrichum acutatum

Plants ◽

10.3390/plants10010114 ◽

2021 ◽

Vol 10 (1) ◽

pp. 114

Author(s):

Armina Morkeliūnė ◽

Neringa Rasiukevičiūtė ◽

Lina Šernaitė ◽

Alma Valiuškaitė

Keyword(s):

Essential Oils ◽

Fungal Pathogens ◽

Control Plant ◽

Antimicrobial Activities ◽

Plant Diseases ◽

Colletotrichum Acutatum ◽

Low Toxicity ◽

Colletotrichum Spp ◽

Biological Protection

The Colletotrichum spp. is a significant strawberry pathogen causing yield losses of up to 50%. The most common method to control plant diseases is through the use of chemical fungicides. The findings of plants antimicrobial activities, low toxicity, and biodegradability of essential oils (EO), make them suitable for biological protection against fungal pathogens. The aim is to evaluate the inhibition of Colletotrichum acutatum by thyme, sage, and peppermint EO in vitro on detached strawberry leaves and determine EO chemical composition. Our results revealed that the dominant compound of thyme was thymol 41.35%, peppermint: menthone 44.56%, sage: α,β-thujone 34.45%, and camphor: 20.46%. Thyme EO inhibited C. acutatum completely above 200 μL L−1 concentration in vitro. Peppermint and sage EO reduced mycelial growth of C. acutatum. In addition, in vitro, results are promising for biological control. The detached strawberry leaves experiments showed that disease reduction 4 days after inoculation was 15.8% at 1000 μL L−1 of peppermint EO and 5.3% at 800 μL L−1 of thyme compared with control. Our findings could potentially help to manage C. acutatum; however, the detached strawberry leaves assay showed that EO efficacy was relatively low on tested concentrations and should be increased.

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Biological Properties of Essential Oils from Thymus algeriensis Boiss

Plants ◽

10.3390/plants10040786 ◽

2021 ◽

Vol 10 (4) ◽

pp. 786

Author(s):

Hamza Ouakouak ◽

Adel Benarfa ◽

Mohammed Messaoudi ◽

Samir Begaa ◽

Barbara Sawicka ◽

...

Keyword(s):

Essential Oils ◽

Micrococcus Luteus ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Cancer Cell Line ◽

Antimicrobial Activities ◽

Biological Properties ◽

Chemical Components ◽

Bornyl Acetate ◽

Thymus Algeriensis

This study describes the chemical composition, antitumor, antioxidant, and antimicrobial activities of the plant Thymus algeriensis Boiss. Essential oils (EOs) were collected in different periods (before, during, and after flowering stage) from the El-Guetfa region, M’sila, Algeria. The EOs extraction was achieved using three distinguishing techniques: hydro (Clevenger trap), steam, and microwave distillations, targeting different aerial parts of the plant (stems, flowers, and leaves). The EOs chemical components were estimated using GC-FID and GC-MS apparatuses. The resulting yield of the extracted oil was moderate and ranged between 0.84 and 1.53% (wt/vol). In total, eighty-five components were identified, in which the oxygenated monoterpenes family formed the main portion, starting from 40.56 up to 70.66%. The obtained essential oil was dominated by five major components that varied from low to quite moderate percentages: camphor (17.45–32.56%), borneol (11.16–22.2%), camphene (7.53–12.86%), 1.8-cineole (5.16–11.21%), and bornyl acetate (3.86–7.92%). The biological results of this oil pointed out that the EOs extracted from the leaves part exposed a weak radical scavenging activity afterward using two well-known antioxidant assays DPPH (IC50 = 8.37 mg/mL) and ABTS (10.84 mg/mL). Meanwhile, this oil presented strong inhibition activity against colon cancer cell line HCT116 (LC50 = 39.8 µg/mL) and a moderate inhibitory against hepatocellular cancer cells HePG2 (LC50 > 100 µg/mL). In addition, this oil antimicrobial activity was quite important against Micrococcus luteus (M. luteus), Staphylococcus aureus CIP 7625, Escherichia coli ATCC 10536, Saccharomyces cerevisiae ATCC 4226, Candida albicans IPA200, Candida tropicalis (Ct), and Candida glabrata (Cg) after using Amoxicillin and Itraconazole as references.

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Essential oils from Ocimum basilicum cultivars: analysis of their composition and determination of the effect of the major compounds on Haemonchus contortus eggs

Journal of Helminthology ◽

10.1017/s0022149x21000080 ◽

2021 ◽

Vol 95 ◽

Author(s):

A.I.P. Sousa ◽

C.R. Silva ◽

H.N. Costa-Júnior ◽

N.C.S. Silva ◽

J.A.O. Pinto ◽

...

Keyword(s):

Essential Oils ◽

Haemonchus Contortus ◽

Anthelmintic Activity ◽

Gastrointestinal Nematodes ◽

Ocimum Basilicum ◽

Alternative Methods ◽

Egg Hatch ◽

Plant Essential Oils ◽

Methyl Chavicol ◽

The Individual

Abstract The continuous use of synthetic anthelmintics against gastrointestinal nematodes (GINs) has resulted in the increased resistance, which is why alternative methods are being sought, such as the use of natural products. Plant essential oils (EOs) have been considered as potential products for the control of GINs. However, the chemical composition and, consequently, the biological activity of EOs vary in different plant cultivars. The aim of this study was to evaluate the anthelmintic activity of EOs from cultivars of Ocimum basilicum L. and that of their major constituents against Haemonchus contortus. The EOs from 16 cultivars as well the pure compound linalool, methyl chavicol, citral and eugenol were used in the assessment of the inhibition of H. contortus egg hatch. In addition, the composition of three cultivars was simulated using a combination of the two major compounds from each. The EOs from different cultivars showed mean Inhibition Concentration (IC50) varying from 0.56 to 2.22 mg/mL. The cultivar with the highest egg-hatch inhibition, Napoletano, is constituted mainly of linalool and methyl chavicol. Among the individual compounds tested, citral was the most effective (IC50 0.30 mg/mL). The best combination of compounds was obtained with 11% eugenol plus 64% linalool (IC50 0.44 mg/mL), simulating the Italian Large Leaf (Richters) cultivar. We conclude that different cultivars of O. basilicum show different anthelmintic potential, with cultivars containing linalool and methyl chavicol being the most promising; and that citral or methyl chavicol isolated should also be considered for the development of new anthelmintic formulations.

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