scholarly journals Extraction of tannins from Punica granatum peel for pharmacological activities

2020 ◽  
Vol 8 (1) ◽  
pp. 144-147
Author(s):  
Sayed Muhammad Amin Sadat ◽  
Anisa Omari
Keyword(s):  
Biological Activities ◽  
Punica Granatum ◽  
Health Food ◽  
Fruit Peel ◽  
Pomegranate Peel ◽  
Pharmacological Activities ◽  
High Antioxidant Activity ◽  
Anti Inflammatory ◽  
Pomegranate Peel Extract ◽  
Punica Granatum L

Punica granatum L. (Pomegranate) is utilized in the treatment of parasitic contamination, loose bowels and ulcers. Extracts of the various parts of P. granum is reported to have anti-inflammatory, antioxidant and anticarcinogenic activities. Tannins are a group of polyphenolic compounds that are widely present in plant regions and possess various biological activities including antimicrobial, anti-parasitic, anti-viral, antioxidant, anti-inflammatory, immunomodulatory activities. This study focused on extraction of tannins from Punica granatum peel which can be further analyzed for its pharmacological activities. The results of this study revealed the abundance of tannins in the pomegranate peel extract. Tannins from pomegranate peel might contribute to the high antioxidant activity of this fruit peel and it is a valuable natural antioxidant source applicable in the health food industry.

scholarly journals A scientific validation of In vitro anti-inflammatory activity of Punica granatum L. by human red blood cell membrane stabilization

2018 ◽  
Vol 6 (7) ◽  
pp. 2430
Author(s):  
Karunakar Kota ◽  
Sandhya Sharma ◽  
Jameela Tahashildar
Keyword(s):  
Methanolic Extract ◽  
Punica Granatum ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Fruit Peel ◽  
Standard Drug ◽  
Membrane Lysis ◽  
Anti Inflammatory ◽  
Punica Granatum L

Background: In recent years there has been growing interest in therapeutic use of natural products, especially those derived from plants. P. granatum is very common dietary ingredient in many parts of India and has remarkable biological and medicinal properties.Methods: In the present study, the methanolic extract of fruit peels of Punica granatum Linn. (MEPG) were investigated for anti-inflammatory activity by simple, reliable, less toxic and less time consuming HRBC membrane stabilization method. The presentation of hypo tonicity induced HRBC membrane lysis was taken a measure of anti-inflammatory activity. Their activities were compared with standard drug diclofenac.Results: The results of the study demonstrated that P. granatum contains active constituents, which possess anti-inflammatory activity which is probably related to the inhibition of prostaglandin synthesis.Conclusions: It is concluded that methanolic extract of P. granatum fruit peel possesses significant anti-inflammatory activity and this is a possible rationale for its folkloric use as an anti-inflammatory agent.

scholarly journals Pengaruh Polivinilpirolidon sebagai Polimer Mukoadhesif terhadap Sifat Fisik Patch Ekstrak Kulit Buah Delima (Punica granatum L.)

2020 ◽  
Vol 1 (2) ◽  
pp. 85
Author(s):  
Rahmat A Hi Wahid
Keyword(s):  
Active Substance ◽  
Contact Time ◽  
Punica Granatum ◽  
Dosage Forms ◽  
Pomegranate Peel ◽  
Mucoadhesive Polymer ◽  
Evaluation Test ◽  
Pomegranate Peel Extract ◽  
Punica Granatum L ◽  
Peel Extract

ABSTRAKBuah delima (Punica granatum L.) secara empiris digunakan untuk mengobati masalah gigi dan mulut seperti sariawan. Pengembangan obat sariawan berbasis herbal dengan bentuk sediaan patch dilakukan untuk mempertahankan waktu kontak antara zat aktif dengan bagian sariawan. Polivinilpirolidon (PVP) digunakan sebagai polimer karena sifatnya mukoadhesif sehingga mampu berikatan pada mukosa mulut. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan PVP sebagai polimer mukoadhesif terhadap karakteristik fisik sediaan patch yang mengandung ekstrak kulit buah delima (EKBD). EKBD diperoleh dengan metode maserasi. Konsentrasi EKBD yang digunakan adalah 5% dan 10%. Sedangkan konsentrasi polimer PVP yang digunakan yaitu 30,29%, 33,04, 48,75%, dan 65,5%. Patch selanjutnya akan di evaluasi fisik yang meliputi keseragaman bobot dan dimensi ketebalan, pH permukaan, swelling, daya lekat, dan waktu lekat. Hasil menunjukkan bahwa patch yang mengandung ekstrak 10% dengan tambahan polimer konsentrasi 30,29% memiliki sifat yang cukup elastis disbanding dengan konsentrasi 48,75% dan 65,5%. Sedangkan uji evaluasi fisik menghasilkan keseragaman bobot dan dimensi yang seragam, pH 6,63, rata-rata indeks swelling 40,69% ± 16, dan rata-rata daya dan waktu lekat 13,50 ± 11,6. Dengan demikian dapat disimpulkan bahwa konsentrasi PVP terbukti berpengaruh pada sifat fisik patch yang dihasilkan. Kata kunci : Polivinilpirolidon; Ekstrak Kulit Buah Delima; Patch.ABSTRACTPomegranate (Punica granatum L.) is empirically used to treat dental and mouth problems such as reccurent aphthous stomatitis (RAS). The development of RAS  therapy based on herbal with patches dosage forms is carried out to maintain the contact time between the active substance and the RAS. Polyvinylpyrrolidone (PVP) is used as a polymer because it is mucoadhesive, so it can bind to the oral mucosa. Aimed to determine the effect of the use of PVP as a mucoadhesive polymer on the physical characteristics of patches preparations containing pomegranate peel extract (PPE). PPE was obtained by maceration method. The PPE concentration used were 5% and 10%. PVP concentration used were 30.29%, 33.04%, 48.75%, and 65.5%. The patches properties such as uniformity of thickness, pH, swelling index, tensile and mucoadhesive strength. The results showed that patches containing 10% extract and PVP concentration of 30.29% had sufficient elastic properties compared to both concentration. While the physical evaluation test obtained uniform weights and dimensions that are uniform, pH 6.63, the average swelling index is 40.69% ± 16, and the tensile and mucoadhesive strength is 13.50 ± 11.6. Altogether, the concentration of PVP is proven to affect the physical properties of the resulting patch.Key word : Polyvinylpyrrolidone; Pomegranate Peel Extract; Patch. 

Physicochemical Properties and Antimicrobial Potential of Green Synthesized Cerium Oxide (CeO2) Nanoparticles from Pomegranate Peel Extract

2021 ◽  
Vol 14 (1) ◽  
pp. 71-78
Keyword(s):  
Antimicrobial Activity ◽  
Biomedical Applications ◽  
Punica Granatum ◽  
Ceo2 Nanoparticles ◽  
X Ray Diffraction ◽  
Fruit Peel ◽  
Pomegranate Peel ◽  
X Ray ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Abstract: Green synthesis of CeO2 Nanoparticles (NPs) with small size and high stability paved the approach to recover and protect the environment by decreasing the use of toxic chemicals and eliminating biological risks in biomedical applications. Peel-mediated synthesis of CeO2 NPs is gaining more importance owing to its easiness and eco-friendliness. In this study, biosynthesis of CeO2 NPs using the fruit peel extract of punica granatum is reported. The synthesized CeO2 NPs are characterized by Powder X-ray Diffraction (PXRD), UV-Diffused Reflection Spectroscopy (UV-DRS), Field Emission Scanning Electron Microscopy (FESEM), Energy Dispersive X-Ray Analysis (EDAX) and antimicrobial activity. The CeO2 NPs show more lethal activity towards gram +ve bacteria than towards gram –ve bacteria. Keywords: Biosynthesis, Optical properties, Antimicrobial activity.

scholarly journals Assessment of the Biological Activity and Phenolic Composition of Ethanol Extracts of Pomegranate (Punica granatum L.) Peels

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5916
Author(s):  
Željka Peršurić ◽  
Lara Saftić Martinović ◽  
Mladenka Malenica ◽  
Ivana Gobin ◽  
Sandra Pedisić ◽  
...  
Keyword(s):  
Biological Activity ◽  
Antiproliferative Activity ◽  
Biological Activities ◽  
Punica Granatum ◽  
Mass Spectrometry Analysis ◽  
Total Phenolic ◽  
Pomegranate Peel ◽  
Spectrometry Analysis ◽  
Ethanol Extracts ◽  
Punica Granatum L

Pomegranate (Punica granatum L.) is a rich source of constituents with confirmed strong biological activities. However, pomegranate peel, which encompasses approximately 30–40% of its weight, is treated as a biological waste. The aim of this paper was to evaluate the potential of pomegranate peel extracts and to propose its functional properties that can be used for development of functional products. Eight ethanol extracts of pomegranate peels (PPEs) were characterized by use of direct infusion quadrupole-time of flight (Q-TOF), and afterwards tested on their antioxidant, antibacterial and antiproliferative activities. Mass spectrometry analysis revealed that the most prevalent compounds in pomegranate peels were punicalagin, granatin and their derivatives. Analysed extracts had high total phenolic contents that ranged from 5766.44 to 10599.43 mg GAE/100 g, and strong antioxidant activity (7551.31–7875.42 and 100.25–176.60 μmol TE/100 g for DPPH and FRAP assays, respectively). The results of biological activity assays showed that all PPEs possessed antibacterial activity, and that S. aureus was the most sensitive specie with minimum inhibitory concentration and minimum bactericidal concentrations ranging from 0.8 to 6.4 mg/mL. Additionally, the analysis of antiproliferative activity revealed high potency of PPEs, as the IC50 values ranged from 0.132 mg/mL to 0.396 mg/mL. Multivariate analysis pointed out the most discriminative metabolites for antioxidant or antiproliferative activity. Overall, the pomegranate peel confirmed to be a highly valuable source of bioactive compounds that could be used to improve the food functional characteristics.

scholarly journals Analisis Kualitatif dan Kuantitatif Tanin Ekstrak Kulit Buah Delima Putih (Punica Granatum L.) Menggunakan Metode Kromatografi Cair Kinerja Tinggi (KCKT)

2020 ◽  
Vol 3 (2) ◽  
Author(s):  
Rahmat A Hi Wahid
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Punica Granatum ◽  
Average Content ◽  
Isocratic Elution ◽  
Uv Detector ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Punica Granatum L ◽  
Peel Extract

ABSTRAKKulit buah delima putih (Punica granatum L.) diketahui mengandung tanin. Tanin merupakan senyawa polifenol yang dapat larut dalam air dan pelarut organik juga dapat mengendapkan protein. Tanin terdapat luas dalam tumbuhan dan digunakan untuk sarana proteksi serangan hewan, bakteri, jamur, serta dapat digunakan sebagai hepatoprotektor. Penelitian ini bertujuan untuk mengetahui cara analisis tanin yang sesuai sehingga diperoleh kadar tanin dalam ekstrak kulit buah delima putih menggunakan metode Kromatografi Cair Kinerja Tinggi (KCKT).Kulit buah delima putih diekstraksi dengan pelarut etanol 70% dengan metode maserasi selama 5 hari. Pengujian kualitatif tanin dilakukan dengan metode Folin-Ciocalteu dan KCKT. Sedangkan pengujian kuantitatif tanin dengan KCKT menggunakan fase diam kolom C18 Octadesyl Silane (ODS) 25 cm x 4,6 mm, fase gerak asam fosfat 0,55% (v/v), kecepatan alir 1,5 mL/menit, elusi isokratik, dan menggunakan detektor spektrofotometer UV (λ 277 nm). Hasil analisis menunjukkan waktu retensi 25,0 menit. Linearitas diperoleh dengan memplotkan luas puncak kromatogram dan konsentrasi isolat menunjukkan korelasi yang baik (r =0,999) dengan persamaan regresi linear y = 645,6x. Kadar rata-rata tanin ekstrak etanol kulit buah delima putih yang ditetapkan dengan KCKT sebesar 2,01%. Dari hasil penelitian ini  dapat disimpulkan bahwa analisis kualitatif tanin dapat dilakukan dengan metode Folin-Ciocalteu dan KCKT sedangkan analisis kuantitatif dapat dilakukan dengan KCKT.Kata kunci : Folin-Ciocalteu, KCKT, Kulit Buah Delima Putih (Punica granatum L.), Tanin.The peel of white pomegranate (Punica granatum L.) is known to contain tannins. These chemical are polyphenols that can be disolved in water in addition to organic solvent and protein. The aim of this study is to quantify and optimize the composition of tannin extracted from pomegranate peel extract by using High-Performance Liquid Chromatography (HPLC).The peel of white pomegranate is extracted by maceration method using 70% of ethanol liquid. HPLC and Folin-Ciocalteu was used as qualitative analyze of tannins, and quantitative analyze by HPLC with Octadesyl Silane (ODS) 25 cm x 4,6 mm as stationary phase C18 column, phosphoric acid 0,55% (v/v) as mobile phase, the flow rate 1,5 mL/minute, isocratic elution and with spectrofotometer UV detector (λ 277 nm). Result showed that a retention time of 25.0 minute.Linearity obtained by plotting the peak area of chromatogram and the concentrate of isolate showed that good correlation (r=0,999) with the equality of linear regretion y = 645,6x. The average content of extract tannins ethanol the peel of white Pomegranate that determinated with HPLC is 2,01%. As a conclusion, the qualitative analyze of tannins can be used by Folin-Ciocalteu and HPLC methods, and quantitative analyze can be used by HPLC.Keywords: Folin-Ciocalteu, HPLC, Tannins, Pomegranate (Punica granatum L.)

scholarly journals Studies on antioxidant activity of red, white, and black pomegranate (Punica granatum L.) peel extract using DPPH radical scavenging method

2021 ◽  
Vol 5 (2) ◽  
pp. 51-55
Author(s):  
Uswatun Chasanah
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Punica Granatum ◽  
Dpph Radical ◽  
Pomegranate Peel ◽  
Antioxidant Power ◽  
Pomegranate Peel Extract ◽  
Dpph Radical Scavenging ◽  
Punica Granatum L ◽  
Peel Extract

Pomegranate (Punica granatum L.) has high antioxidant activity. In Indonesia, there are red pomegranate, white pomegranate, and black pomegranate. The purpose of this study was to determine the antioxidant activity of red pomegranate peel extract, white pomegranate peel extract, and black pomegranate peel extract. The extracts prepared by ultrasonic maceration in 96% ethanol, then evaporated until thick extract was obtained and its antioxidant activity was determined using the DPPH radical scavenging method. This study showed that all pomegranate peel extract varieties have potent antioxidant activity and the black pomegranate peel extract has the highest antioxidant power.

PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT ACTIVITY AND ACUTE ORAL TOXICITY OF POMEGRANATE (PUNICA GRANATUM L.) FRUIT PEEL EXTRACT

2019 ◽  
pp. 7-14
Author(s):  
Hai Trieu Ly ◽  
Tuan Anh Vo ◽  
Viet Hong Phong Nguyen ◽  
Thi My Sa Pham ◽  
Bich Thao Lam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Punica Granatum ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Fruit Peel ◽  
Total Polyphenol ◽  
Punica Granatum L

Background: The natural antioxidants have an important role in the prevention of many diseases. The aim of study is to investigate phytochemical components, antioxidant activity and acute oral toxicity of Pomegranate (Punica granatum L.) fruit peel (PFP) extract. Materials and methods: Phytochemicals of PFP were determined by qualitative chemical tests, thin layer chromatography, total polyphenol and flavonoid contents. The PFP extract was evaluated for antioxidant activity by DPPH assay and MDA assay. In vivo acute oral toxicity test was conducted using Karber-Behrens method to determine LD50. Results: Results illustrated that PFP mainly contains flavonoids, alkaloids, tannins, triterpenes, saponins, and coumarins. PFP extract exhibited the total polyphenol and flavonoid contents with 189.97 mg gallic acid equivalent/g dry weight and 9.42 mg quercetin equivalent/g dry weight, respectively. The DPPH free radical scavenging and anti-lipid peroxidation activities of PFP extract were expressed with IC50 value of 4.80 μg/mL and 0.38 μg/ mL, sequentially. Simultaneously, the Dmax (the maximum dose administered to mice that no toxicity was observed) of PFP extract was determined to be 21.28 g/kg, equivalent to 35.64 g dried herb. Conclusion: The PFP extract is relatively safe and revealed high antioxidant activity. Key words: Punica granatum L.; polyphenols; flavonoids; gallic acid; quercetin; antioxidant activity; acute oral toxicity

Optimization of subcritical water extraction of phenolic antioxidants from pomegranate (Punica granatum L.) peel by response surface methodology

2017 ◽  
Vol 9 (32) ◽  
pp. 4647-4656 ◽  
Author(s):  
Linlin Yan ◽  
Yungang Cao ◽  
Guangyao Zheng
Keyword(s):  
Response Surface Methodology ◽  
Subcritical Water ◽  
Punica Granatum ◽  
Water Extraction ◽  
Phenolic Antioxidants ◽  
Subcritical Water Extraction ◽  
Extraction Technology ◽  
Pomegranate Peel ◽  
Efficient Extraction ◽  
Punica Granatum L

Subcritical water extraction (SWE), a ‘green’ and efficient extraction technology, was applied to extract phenolic antioxidants from pomegranate peel in this study.

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