Chemical Composition and Antibacterial Activity of Lippia multiflora Moldenke Essential Oil from Different Regions of Angola

The purpose of the study was to determine the chemical composition and antibacterial activity of Lippia multiflora Moldenke essential oils (EOs) collected in different regions of Angola. Antibacterial activity was evaluated using the agar wells technique and vapour phase test. Analysis of the oils by GC/MS identified thirty-five components representing 67.5 to 100% of the total oils. Monoterpene hydrocarbons were the most prevalent compounds, followed by oxygenated monoterpenes. The content of the compounds varied according to the samples. The main components were Limonene, Piperitenone, Neral, Citral, Elemol, p-cymene, Transtagetone, and Artemisia ketone. Only one of the eleven samples contained Verbenone as the majority compound. In the vapour phase test, a single oil was the most effective against all the pathogens studied. The principal component analysis (PCA) and hierarchical cluster analysis (HCA) of components of the selected EOs and inhibition zone diameter values of agar wells technique allowed us to identify a variability between the plants from the two provinces, but also intraspecific variability between sub-groups within a population. Each group of essential oils constituted a chemotype responsible for their bacterial inhibition capacity. The results presented here suggest that Angolan Lippia multiflora Moldenke has antibacterial properties and could be a potential source of antimicrobial agents for the pharmaceutical and food industry.

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Potentially Effective and Safe Anti-Helicobacter pylori Natural Products: Chemometric Study

Revista de Chimie ◽

10.37358/rc.20.6.8192 ◽

2020 ◽

Vol 71 (6) ◽

pp. 267-273 ◽

Cited By ~ 1

Author(s):

Branislava D. Kocic ◽

Dobrila M. Stankovic Dordevic ◽

Marija V. Dimitrijevic ◽

Marija S. Markovic ◽

Dragoljub L. Miladinovic

Keyword(s):

Helicobacter Pylori ◽

Antibacterial Activity ◽

Natural Products ◽

Chemical Composition ◽

Essential Oils ◽

Principal Component ◽

Synergistic Activity ◽

Agglomerative Hierarchical Clustering ◽

Naturally Occurring ◽

H Pylori

The susceptibility of Helicobacter pylori to three essential oils (EOs), 12 naturally occurring monoterpene hydrocarbons, oxygenated and phenolic monoterpenes and three reference antibiotics were studied. Classification and comparison of essential oils and monoterpenes on the basis of their chemical composition and antibacterial activity were made by the utilization of principal component analyses (PCA) and agglomerative hierarchical clustering (AHC). The most abundant compound in the Thymus glabrescens Willd. and Thymus pulegioides L. EOs is geraniol (33.8% and 52.5%), while the main constituent in Satureja kitaibelii Wierzb. ex Heuff. EO is limonene (16,1%). The compound that was the most active against H. pylori was carvacrol. EOs of T. glabrescens and S. kitaibelii exhibit higher antibacterial ability in comparison with all monoterpenes, except carvacrol, probably based on the concept of synergistic activity of essential oil components. PCA separated essential oils based on chemical composition and explain 96.5% of the total variance in the first two principal components. Essential oils, phenolic monoterpenes and two antibiotics were classified in the same sub-cluster within AHC analyses. EOs of T. glabrescens and S. kitaibelii can be used to treat infections caused by H. pylori, as a potentially effective, cheap and safe natural products. Further research of antibacterial activity of selected monoterpenes, essential oils and standard antibiotic combinations, as well as clinical study are required.

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Effects of Gender and Geographical Origin on the Chemical Composition and Antiradical Activity of Baccharis myriocephala and Baccharis trimera

Foods ◽

10.3390/foods9101433 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1433

Author(s):

Suzana Struiving ◽

Ana Carolina Mendes Hacke ◽

Edésio Luiz Simionatto ◽

Dilamara Riva Scharf ◽

Cláudia Vargas Klimaczewski ◽

...

Keyword(s):

Chemical Composition ◽

Ethyl Acetate ◽

Essential Oils ◽

Antiradical Activity ◽

Principal Component ◽

Hierarchical Cluster ◽

Gas Chromatography Mass Spectrometry ◽

Array Detector ◽

Chemical Compositions ◽

Baccharis Trimera

This study aimed to characterize and compare essential oils and ethyl acetate fractions obtained in basic and acidic conditions from both male and female Baccharis species (Baccharis myriocephala and Baccharis trimera) from two different Brazilian regions. Samples were characterized according to their chemical compositions and antiradical activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Principal component analysis (PCA) provided a clear separation regarding the chemical composition of essential oils from the samples obtained from different regions by using gas chromatography–mass spectrometry with flame-ionization detection (GC-MS-FID). PCA also revealed that gender and region of plant collections did not influence the chemical composition and antiradical activity of ethyl acetate fractions, which was corroborated with hierarchical cluster analysis (HCA) data. High performance liquid chromatography with diode-array detector (HPLC-DAD) identified significant quantities of flavonoids and phenolic acids in the fractions obtained in basic and acidic fractions, respectively. The obtained results clearly demonstrated that the geographical region of plant collection influenced the chemical composition of essential oils from the studied Baccharis species. Moreover, the obtained fractions were constituted by several antiradical compounds, which reinforced the usage of these species in folk medicine.

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Chemical composition and antibacterial activity of essential oils of two species of Lamiaceae against phytopathogenic bacteria

Polish Journal of Microbiology ◽

10.33073/pjm-2015-018 ◽

2015 ◽

Vol 64 (2) ◽

pp. 121-127 ◽

Cited By ~ 17

Author(s):

ARZU GORMEZ ◽

SEDAT BOZARI ◽

DERYA YANMIS ◽

MEDİNE GULLUCE ◽

FİKRETTİN SAHIN ◽

...

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Control Plant ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Plant Diseases ◽

Phytopathogenic Bacteria ◽

Dilution Assay ◽

Diffusion Assay

In this study, we aimed to determine chemical composition and antibacterial activities of Satureja hortensis and Calamintha nepeta against to 20 phytopathogenic bacteria causing serious crop loss. The essential oils of S. hortensis and C. nepeta were isolated by the hydrodistillation method and the chemical composition of the essential oils were analyzed by GC-MS. The antibacterial properties of the essential oils were evaluated against 20 phytopathogenic bacteria through Disc diffusion assay and micro dilution assay. The results revealed that the essential oils of S. hortensis and C. nepeta have significant antibacterial activity. Furthermore, the findings of the study are valuable for future investigations focusing on the alternative natural compounds to control plant diseases.

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Seasonal Effect on the Chemical Composition, Insecticidal Properties and Other Biological Activities of Zanthoxylum leprieurii Guill. & Perr. Essential Oils

Foods ◽

10.3390/foods9050550 ◽

2020 ◽

Vol 9 (5) ◽

pp. 550 ◽

Cited By ~ 5

Author(s):

Evelyne Amenan Tanoh ◽

Guy Blanchard Boué ◽

Fatimata Nea ◽

Manon Genva ◽

Esse Leon Wognin ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Biological Activities ◽

Principal Component ◽

Hierarchical Cluster ◽

Wild Plant ◽

Gas Chromatography Mass Spectrometry ◽

Seasonal Effect ◽

The Impact

This study focused, for the first time, on the evaluation of the seasonal effect on the chemical composition and biological activities of essential oils hydrodistillated from leaves, trunk bark and fruits of Zanthoxylum leprieurii (Z. leprieurii), a traditional medicinal wild plant growing in Côte d’Ivoire. The essential oils were obtained by hydrodistillation from fresh organs of Z. leprieurii growing on the same site over several months using a Clevenger-type apparatus and analyzed by gas chromatography-mass spectrometry (GC/MS). Leaf essential oils were dominated by tridecan-2-one (9.00 ± 0.02–36.80 ± 0.06%), (E)-β-ocimene (1.30 ± 0.50–23.57 ± 0.47%), β-caryophyllene (7.00 ± 1.02–19.85 ± 0.48%), dendrolasin (1.79 ± 0.08–16.40 ± 0.85%) and undecan-2-one (1.20 ± 0.03–8.51 ± 0.35%). Fruit essential oils were rich in β-myrcene (16.40 ± 0.91–48.27 ± 0.26%), citronellol (1.90 ± 0.02–28.24 ± 0.10%) and geranial (5.30 ± 0.53–12.50 ± 0.47%). Tridecan-2-one (45.26 ± 0.96–78.80 ± 0.55%), β-caryophyllene (1.80 ± 0.23–13.20 ± 0.33%), α-humulene (4.30 ± 1.09–12.73 ± 1.41%) and tridecan-2-ol (2.23 ± 0.17–10.10 ± 0.61%) were identified as major components of trunk bark oils. Statistical analyses of essential oil compositions showed that the variability mainly comes from the organs. Indeed, principal component analysis (PCA) and hierarchical cluster analysis (HCA) allowed us to cluster the samples into three groups, each one consisting of one different Z. leprieurii organ, showing that essential oils hydrodistillated from the different organs do not display the same chemical composition. However, significant differences in essential oil compositions for the same organ were highlighted during the studied period, showing the impact of the seasonal effect on essential oil compositions. Biological activities of the produced essential oils were also investigated. Essential oils exhibited high insecticidal activities against Sitophilus granarius, as well as antioxidant, anti-inflammatory and moderate anti-plasmodial properties.

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The Essential Oil Compositions of Three Teucrium Taxa Growing Wild in Sicily: HCA and PCA Analyses

Molecules ◽

10.3390/molecules26030643 ◽

2021 ◽

Vol 26 (3) ◽

pp. 643

Author(s):

Giorgia Catinella ◽

Natale Badalamenti ◽

Vincenzo Ilardi ◽

Sergio Rosselli ◽

Laura De Martino ◽

...

Keyword(s):

Cluster Analysis ◽

Chemical Composition ◽

Essential Oils ◽

Principal Component ◽

Hierarchical Cluster ◽

The Other ◽

Qualitative And Quantitative ◽

Geographical Variations ◽

Sesquiterpene Hydrocarbons ◽

Principal Component Analyses

The chemical composition and the qualitative and quantitative variability of the essential oils of three taxa belonging to the Teucrium genus were studied. The investigated taxa, that grow wild in Sicily, were Teucrium flavum L. (section Chamaedrys (Mill.) Scheb.), Teucrium montanum and Teucrium capitatum L. of section Polium (Mill.) Scheb. Essential oils were extracted by hydrodistillation and analyzed by GC-MS. In total, 74 compounds were identified. Sesquiterpene hydrocarbons were found to be the main group for T. flavum (48.3%). T. capitatum consisted essentially of monoterpene hydrocarbons (72.7%), with α-pinene (19.9%), β-pinene (27.6%) and sylvestrene (16.6%) as the most abundant compounds whereas ledene oxide (12.1%), epiglobulol (13.5%) and longifolenaldehyde (14.5%) were identified as the main constituents among the oxygenated sesquiterpenes (63.5%) of T. montanum. Furthermore, a complete literature review on the composition of the essential oils of all the other accessions of these Teucrium taxa, studied so far, was performed. Hierarchical Cluster Analysis (HCA) and Principal Component Analyses (PCA) were used in order to demonstrate geographical variations in the composition of the essential oils.

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Anti-bacterial properties and GC-MS analysis of extracts and essential oils of selected plant product

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f160105 ◽

2018 ◽

Vol 16 (1) ◽

pp. 44-58

Author(s):

OMBUNA DINAH NYAITONDI ◽

RUTH NDUTA WANJAU ◽

HUDSON NYAMBAKA ◽

AHMED HASSANALI

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Bacterial Infections ◽

Antimicrobial Agents ◽

Antibacterial Properties ◽

Salmonella Typhi ◽

Retention Indices ◽

Methanol Extracts ◽

Plant Product ◽

Ms Analysis

Nyaitondi OD, Wanjau R, Nyambaka H, Hassanali A. 2018. Anti-bacterial properties and GC-MS analysis of extracts and essential oils of selected plant product. Biofarmasi J Nat Prod Biochem 16: 44-58. Plants are traditionally used for the treatment of bacterial infections though they are not clinically regulated due to lack of awareness and sufficient data to support the reported therapeutic claims. Some plants used as food and vegetables are hardly considered in such studies. This study aimed to investigate the antibacterial properties associated with garlic, ginger, turmeric, lemon, and onion in the form of juices, methanol extracts, and essential oils. These materials were tested against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Salmonella typhi. Identification of suspected antibacterial compounds was made by comparison of retention indices and the mass spectra with those in National Institute of Standards and Technology (NIST) libraries using GC-MS analyses. Garlic juice was bactericidal against all tested strains. Lemon/garlic juice exhibited significantly higher activity against E. coli and S. typhi. Turmeric/lemon/garlic methanol extracts blend was most active against S. aureus. Preliminary screening of the essential oils indicated significant antibacterial activity of lemon/garlic essential oil blend against P. aeruginosa. GC-MS analysis of the active samples confirmed the presence of compounds containing-OOH,-OH,-N,-Cl,-F,-NH2 and-S groups which are associated with bacterial inhibition in conventional antibiotics. The 10 major constituents obtained from samples suspected to contain antibacterial activity, include limonene; 3-vinyl-1,2-dithiacyclohex-4- ene; α-zingiberene; diallyl disulphide; 2-butanone,4-(-hydroxy-3-methoxyphenyl); 3-chlorothiophene; methanehydrazonic acid,N-[3- (methylthio)-1,-2,4-thiadiazol-5-yl]-,ethyl ester; n-hexadecanoic acid; γ-sitosterol and propanamide,2-amino-3-phenyl. Juices of garlic, lemon and lemon/garlic blend were found to be active against one or more of the bacteria tested unlike methanol extracts and essential oils, and they should be used in raw form as heating and drying is likely to render them inactive. Further studies on methanol extract and fresh juice of lemon/garlic blend need to be undertaken to elucidate the active principles in these extracts and may lead to the discovery of novel antimicrobial agents and models for the new generation of synthetic antibiotics.

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Chemical Composition, Antioxidant, and Antimicrobial Activities of the Essential Oils From Аrtemisia annua L. Growing Wild in Tajikistan

Natural Product Communications ◽

10.1177/1934578x20927814 ◽

2020 ◽

Vol 15 (5) ◽

pp. 1934578X2092781

Author(s):

Farukh S Sharopov ◽

Aminjon Salimov ◽

Sodik Numonov ◽

Abduahad Safomuddin ◽

Mahinur Bakri ◽

...

Keyword(s):

Mass Spectrometry ◽

Antioxidant Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Hierarchical Cluster ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Aerial Parts ◽

Antioxidant And Antimicrobial Activities

The aerial parts of Аrtemisia annua L. were collected from Varzob, Rudaki, and Hisor regions of Tajikistan. The essential oil was obtained by hydrodistillation and analyzed by gas chromatography–mass spectrometry. The essential oils of aerial parts of А. annua were dominated by the monoterpenoids like camphor (32.5%-58.9%), 1,8-cineole (13.7%-17.8%), camphene (4.5%-8.4%), and α-pinene (1.9%-7.3%). Hierarchical cluster analysis of A. annua essential oils indicated the existence of 3 A. annua chemotypes: camphor/1,8-cineole, camphor, and artemisia ketone. The essential oils of А. annua show weak antioxidant activity and average antibacterial activity. In our opinion, the antibacterial activity of А. annua essential oils is related to the presence of 1,8-cineole. To our best knowledge, this is the first report concerning the chemical composition, chemotypic variation, antioxidant, and antimicrobial activities of the essential oils obtained from the aerial parts of А. annua, growing wild in Tajikistan.

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Correlation between Chemical Composition of Greek Essential Oils and their Antibacterial Activity against Food-borne Pathogens

Natural Product Communications ◽

10.1177/1934578x0700200413 ◽

2007 ◽

Vol 2 (4) ◽

pp. 1934578X0700200 ◽

Cited By ~ 4

Author(s):

Nikos G. Chorianopoulos ◽

Epameinontas T. Evergetis ◽

Nektarios Aligiannis ◽

Sofia Mitakou ◽

George-John E. Nychas ◽

...

Keyword(s):

Gas Chromatography ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Salmonella Enteritidis ◽

Principal Component ◽

Antibacterial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Food Borne

The compositions of forty-nine essential oils obtained from plants growing in areas of biodiversity in Greece were determined by means of gas chromatography and gas chromatography–mass spectrometry. The in vitro antibacterial activities of the oils were evaluated against a panel of five common food-borne bacteria ( Escherichia coli 0157:H7 NCTC 12900, Salmonella Enteritidis PT4, Staphylococcus aureus ATCC 6538, Listeria monocytogenes Scott A and Bacillus cereus FSS 134). Monoterpene hydrocarbons, phenolic monoterpenes and alcohols were the major constituents of the oils, but their concentrations varied greatly between the samples examined. The essential oils obtained from the species endemic to Greece ( Satureja and Origanum) had the most potent bactericidal properties. The chemical composition data were statistically processed through Principal Component Analysis (PCA) and correlated with the antibacterial activities, allowing a rough estimation of the potential of the essential oils antibacterial activity.

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Composition and Chemical Variability of Myrtus communis Leaf Oil From Northwestern Algeria

Natural Product Communications ◽

10.1177/1934578x19850030 ◽

2019 ◽

Vol 14 (5) ◽

pp. 1934578X1985003

Author(s):

Chahrazed Bekhechi ◽

Charaf Eddine Watheq Malti ◽

Maghnia Boussaïd ◽

Imane Achouri ◽

Karima Belilet ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Biological Activities ◽

Principal Component ◽

Hierarchical Cluster ◽

Retention Indices ◽

Resonance Spectroscopy ◽

Myrtus Communis ◽

Leaf Oil ◽

Pharmaceutical Industries

Myrtus communis L. is a shrub growing wild in most countries all around the Mediterranean Sea and myrtle leaf oil is useful in the perfumery and pharmaceutical industries. Nowadays, “chemotyped” essential oils are more and more appreciated. Myrtle oil from eastern and central-north Algeria belong to the “α-pinene-cineole” chemotype. In contrast, Moroccan myrtle oil displayed appreciable content of myrtenyl acetate. Therefore, it appeared crucial to investigate the composition of myrtle leaf oil from northwestern Algeria in order to ensure the homogeneity of the composition of Algerian myrtle leaf oil. The chemical composition of 42 oil samples of M. communis isolated from leaves collected in five locations from Tlemcen Province was investigated by gas chromatography (GC) in combination with retention indices, GC-mass spectroscopy, and 13C nuclear magnetic resonance spectroscopy. Yields ranged between 0.13% and 1.04% (w/w). The chemical composition of the oils was largely dominated by monoterpene hydrocarbons, with α-pinene (24.3-59.0%) and 1,8-cineole (13.2-49.5%) being the major compounds followed by limonene (3.2-19.8%). The 42 compositions were submitted to statistical analyses. The combination of hierarchical cluster analysis and principal component analysis allowed the distinction of two groups, the second group being subdivided into two subgroups. Groups and subgroups were differentiated with respect to their contents of α-pinene and 1,8-cineole and, to a lesser extent, of limonene. This study ensures that Algerian myrtle leaf oil belongs to the “α-pinene-cineole” chemotype which displays various biological activities. The observed homogeneity could be a positive point to join the international market of essential oils.

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Chemometric Comparison and Classification of Some Essential Oils Extracted from Plants Belonging to Apiaceae and Lamiaceae Families Based on Their Chemical Composition and Biological Activities

Molecules ◽

10.3390/molecules23092261 ◽

2018 ◽

Vol 23 (9) ◽

pp. 2261 ◽

Cited By ~ 9

Author(s):

Cristina Semeniuc ◽

Maria-Ioana Socaciu ◽

Sonia Socaci ◽

Vlad Mureșan ◽

Melinda Fogarasi ◽

...

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Biological Activities ◽

Principal Component ◽

Total Phenolic ◽

Chemometric Methods ◽

Weak Antibacterial Activity

This study is focused on the comparison and classification of parsley, lovage, basil, and thyme essential oils (EOs) based on their chemical composition, total phenolic content, antioxidant and antibacterial activities by using appropriate chemometric methods: Principal component analysis (PCA) and hierarchical cluster analysis (HCA). The results showed that parsley, lovage, and thyme EOs are rich in monoterpene hydrocarbons, but basil EO is rich in oxygenated monoterpenes and phenylpropanoids, and that both PCA and HCA separated essential oils into two main groups of which one contains two sub-groups. β-Phellandrene was the major component identified in parsley and lovage EOs, estragole was the major component in basil EO, and p-cymene was the major component in thyme EO. Thyme EO showed the highest level of total phenolics, the highest antioxidant capacity, and exhibited the stronger antibacterial activity, results that were emphasized by both chemometric methods used. Among tested essential oils, the one of parsley was distinguished by a low total TPC, weak antioxidant activity, and weak antibacterial activity against S. enteritidis (ATCC 13076); lovage EO by low TPC, weak antioxidant activity, but moderate antibacterial activity; and basil EO by low TPC, moderate antioxidant activity, and weak antibacterial activity against L. monocytogenes (ATCC 19114).

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