scholarly journals Phytochemical Analysis, Antispasmodic, Myorelaxant, and Antioxidant Effect of Dysphania ambrosioides (L.) Mosyakin and Clemants Flower Hydroethanolic Extracts and Its Chloroform and Ethyl Acetate Fractions

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7300
Author(s):  
Fahd Kandsi ◽  
Raffaele Conte ◽  
Mohamed Marghich ◽  
Fatima Zahra Lafdil ◽  
Mohamed F. Alajmi ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Folk Medicine ◽  
Reducing Power ◽  
Beta Carotene ◽  
Antioxidant Effect ◽  
Perennial Herb ◽  
Phytochemical Analysis ◽  
Traditional Use ◽  
Ms Analysis ◽  
Hydroethanolic Extract

Dysphania ambrosioides (L.) Mosyakin and Clemants is an annual or ephemeral perennial herb used traditionally in the Mediterranean region in folk medicine to treat various illnesses, including those related to the digestive system. This study aims to assess the antispasmodic, myorelaxant, and antioxidant effects of D. ambrosioides flower hydroethanolic extract and its chloroform and ethyl acetate fractions in a comparative study to evaluate the result of the extraction type on the potential activity of the extract. Both rat and rabbit jejunum were used to evaluate the antispasmodic and myorelaxant effect, while the antioxidant effect was evaluated using DPPH, a ferric reducing power assay, and a beta-carotene bleaching test. LC/MS-MS analysis was carried out to reveal the composition of the different types of extract. Following the results, the hydroethanolic extract showed a significant myorelaxant effect (IC50 = 0.39 ± 0.01 mg/mL). Moreover, it was shown that the hydroethanolic extract demonstrated the best antispasmodic activity (IC50 = 0.51 ± 0.05 mg/mL), followed by the ethyl acetate (IC50 = 4.05 ± 0.32 mg/mL) and chloroform (IC50 = 4.34 ± 0.45 mg/mL) fractions. The antioxidant tests showed that the hydroethanolic extract demonstrated high antioxidant activity, followed by the ethyl acetate and chloroform fractions. The LC/MS-MS analysis indicates that the plant extract was rich in flavonoids, to which the extract activity has been attributed. This study supports the traditional use of this plant to treat digestive problems, especially those with spasms.

scholarly journals PRELIMINARY SCREENING OF PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT, AND ANTI-BACTERIAL ACTIVITIES IN THE METHANOLIC LEAF EXTRACT OF MELIOSMA SIMPLICIFOLIA (L.)

2021 ◽  
pp. 100-104
Author(s):  
PAVITHRA S ◽  
SEKAR T
Keyword(s):  
Leaf Extract ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Hot Water ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Dpph Scavenging

Objective: In the present study is investigated of phytochemicals and antioxidant activities of the leaf extracts from Meliosma simplicifolia (L.). Methods: The seaweed sample was subjected to extraction with solvents of different polarities (hot water, methanol, acetone, chloroform, and petroleum ether) and screened for phytochemicals according to standard methods. The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, and superoxide radical (O2•) scavenging activity. The ICPMS and GC-MS analysis of the methanol leaf extract of M. simplicifolia was revealed the presence of antibacterial. Results: Phytochemical analysis revealed the presence of Preliminary metabolites Quantitative studies of estimated phenol, flavonoid and tannin, as for the methanol extract of stem showed the highest content of phenolic compounds (40.71±0.94 GAE mg/100). Antioxidant activities were concluded the estimation M. simplicifolia leaf for as followed the studies. In leaf the highest DPPH scavenging activity (132.3 μg/ml), ICP-MS analysis of the leaf extract showed the presence minerals such as Mg, Fe, Cu, Na, and Zn in excess. The leaf extract of the plant was also tested for its antibacterial activity and results confirmed that it has potential activity. Conclusion: The preliminary studies in the methanol extract of the leaf of M. simplicifolia are suggestive of the antibacterial potentials of the methanol extract of leaves of M. simplicifolia.

scholarly journals Studies on In Vitro Antioxidant Activity of Methanolic Extract and Fractions of Ficus Hispida Lin. Fruits

2016 ◽  
Vol 8 (3) ◽  
pp. 371-380 ◽  
Author(s):  
M. S. Hossain ◽  
S. Parvin ◽  
S. Dutta ◽  
M. S. I. Mahbub ◽  
M. E. Islam
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Traditional Use ◽  
Total Antioxidant ◽  
Ficus Hispida

The present study was designed to confirm the traditional use of the fruits of Ficus hispida Linn. (Moraceae) as an antioxidant agent. Fruits of the plant extracted with methanol and crude methanol extract (CME) were further fractionated with n-hexane, chloroform, and ethyl acetate. All the fractions, n-hexane (NHF), chloroform (CHF), ethyl acetate (EAF), aqueous (AQF) and CME were preliminary screened for in vitro antioxidant activity and total phenolic and total flavonoid content. In DPPH radical scavenging assay, CME exhibited highest scavenging activity (IC50 = 11.20 µg/mL) as compared to other fractions. In this assay, IC50 of reference standard BHT was 5.10 µg/mL. The reducing power of the samples was in the order as AQF > CME > CHF > EAF > NHF. The results for hydrogen peroxide scavenging activity indicated that CME, EAF and AQF had almost the same scavenging activity except NHF. Total antioxidant capacity of CME and other fractions were ranked as CHF > AQF > CME > EAF > NHF.  In the assay of antioxidant constituents (total phenol and total flavonoids content), the CME had highest phenolic and flavonoids content. The results indicate that Ficus hispida fruits could be considered as a potential source of natural antioxidant.

scholarly journals LC-ESI-MS/MS Phenolic Profile of Volutaria lippii (L.) Cass. Extracts and Evaluation of Their In Vitro Antioxidant, Antiacetylcholinesterase, Antidiabetic, and Antibacterial Activities

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Hichem Ben Salah ◽  
Slim Smaoui ◽  
Raed Abdennabi ◽  
Noureddine Allouche
Keyword(s):  
Ethyl Acetate ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Perennial Herb ◽  
Total Phenolic ◽  
Aqueous Fraction ◽  
Esi Ms ◽  
Wide Range ◽  
Phenolic And Flavonoid

Volutaria lippii (L.) Cass., an indigenous perennial herb from the Tunisian flora, belongs to the medicinally important genus Volutaria Cass. (Asteraceae) which comprises eighteen species widely distributed in the Irano-Turanian and Mediterranean Basin. In this study, five different extracts from Tunisian Volutaria lippii (L.) Cass. were evaluated for their in vitro antioxidant, antiacetylcholinesterase, antidiabetic, and antibacterial activities as well as for their total phenolic and flavonoid contents. The results indicated that the ethyl acetate and aqueous fractions have the highest levels in phenolic and flavonoid contents and showed remarkable antioxidant activities using DPPH (IC50= 11.50±0.57 and 28.81±1.35μg/mL, respectively), total antioxidant capacity (105.21±0.01 and 98.77±0.02 mg vitamin E/g extract, respectively), and reducing power (EC50= 55.40±2.00 and 66.65±1.40 μg/mL, respectively) methods. Furthermore, they exhibited noticeable antiacetylcholinesterase and antidiabetic activities and a moderate antibacterial effect when compared to that of standards. Principal component analysis allowed highlighting the ethyl acetate extract for its interesting acetylcholinesterase enzyme (AChE) and alpha-amylase activities and the aqueous fraction for its remarkably antibacterial activity, and their richness in phytochemical content. Interestingly, the LC-ESI-MS/MS analyses of both fractions allowed the identification of ten phenolic acids and eight flavonoids. The 3-O-caffeoylquinic and 3,4-di-O-caffeoylquinic acids constituted the most abundant components in the two fractions. Taken together, these findings demonstrated, for the first time, that Volutaria lippii (L.) Cass. is a potential source of biological active compounds which could be used in a wide range of fields, namely, nutrition and complementary pharmacological drug.

scholarly journals PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF ROOT EXTRACTS OF PARKIA BIGLANDULOSA (WIGHT&ARN.)

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Yogashree G.D. ◽  
Shivakumar Singh P ◽  
Shrishail H.C
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Quantitative Estimation ◽  
Petroleum Ether Extract ◽  
Research Work ◽  
Bioactive Metabolites ◽  
Phytochemical Analysis ◽  
Distilled Water ◽  
Root Extracts ◽  
Ms Analysis

The current research work estimated to explore the qualitative and quantitative analysis of the major bioactive constituents of medicinally imperative plant Parkia biglandulosa in, the five solvent (petroleum ether, chloroform, ethyl acetate, methanolic and distilled water) root extracts of Parkia biglandulosa were predictable and characterized using GC-MS analysis. The explorations were accepted out in terms of five solvent extractions, intact extractive values, qualitative analysis, quantitative estimation and characterization of phytochemicals. The percentage value of yield extraction in petroleum ether extract was 34.74%, chloroform extract was 24.2%,ethyl acetate extract was 25%, methanol was 50% and distilled water was 54%. The preliminary phytochemical analysis showed the presence of alkaloids, flavonoids, terpenoids & phenolic compounds. The total metabolites content of five solvent extracts were compared with standards. The GC-MS analysis reveals the presence of various metabolites in three solvent(petroleum ether, ethyl acetate, methanol) extracts. It signifies that results revealed the presence of various bioactive metabolites which could be demoralized for their credible solicitations for therapeutic resolves.

scholarly journals PHYTOCHEMICAL PROFILING USING LC-Q-TOF-MS ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF A RARE SALT-SECRETING MANGROVE AEGIALITIS ROTUNDIFOLIA ROXB. LEAVES EXTRACT

2019 ◽  
pp. 37-47
Author(s):  
Debjit Ghosh ◽  
Sumanta Mondal ◽  
Prof. Karipeddi Ramakrishna
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
In Vitro Models ◽  
Phytochemical Analysis ◽  
Ms Analysis ◽  
Aegialitis Rotundifolia ◽  
Tof Ms

Objective: The present work deals with the qualitative study of the phytoconstituents present in Aegialitis rotundifolia Roxb., ethanolic leaves extract and evaluate its antioxidant properties in vitro. Methods: The qualitative phytochemical analysis of the extract was performed first using preliminary phytochemical tests and then by liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS). The antioxidant properties were investigated comprehensively using seven in vitro models viz., 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, nitric oxide (NO) scavenging, hydrogen peroxide (H2O2) scavenging, superoxide (SOD) radical scavenging, lipid peroxidation (LPO) assay, reducing power (RP), and total antioxidant activity. Results: The preliminary phytochemical analysis revealed the presence of several important phytochemical groups whereas the LC-Q-TOF-MS analysis detected 25 phytoconstituents in the extract mostly belonging to flavonoids and alkaloids. The test extract showed strong dose-dependent antioxidant activity in all the seven in vitro models, however, the activity of the extracts was slightly lower compared to the reference standard ascorbic acid. Conclusion: The test extract showed strong antioxidant properties which could be possibly due to the phytoconstituents detected in the extract.

scholarly journals PHYTOCHEMICAL COMPOUNDS AND ANTIOXIDANT ACTIVITY OF YELLOW VELVETLEAF FRUIT (LIMNOCHARIS FLAVA) EXTRACT

2019 ◽  
pp. 55-57
Author(s):  
ACE BAEHAKI ◽  
SHANTI DWITA LESTARI ◽  
NORYATI SIREGAR
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Power Method ◽  
Activity Assessment ◽  
Sample Extraction ◽  
Phytochemical Compounds

Objective: The purpose of this research was to observe the content of phytochemical compound and antioxidant activity of yellow velvetleaf fruit (Limnocharis flava) extract. Methods: Research consisted of several stages, including sampling, sample preparation, sample extraction, calculation of yield extract, phytochemical analysis (flavonoids, steroids, alkaloids, saponins, and triterpenoids), and antioxidant activity assessment using 2’,2’-diphenyl-1-picrylhydrazyl and iron-reducing power method. Results: The n-hexane and methanol extract contained flavonoids, saponins, and triterpenoids, while flavonoids and triterpenoids were detected on ethyl acetate extract. The IC50 of yellow velvetleaf extract with n-hexane, ethyl acetate, and methanol was 3321.67 ppm, 1439.24 ppm, and 96.0 ppm, respectively. The methanol extract had the highest iron-reducing power with the average absorbance of 0.588 followed by the ethyl acetate extract with the average absorbance of 0.195 and n-hexane extract had lowest with the average absorbance of 0.171. Conclusion: The best solvent to extract the yellow velvetleaf and have highest antioxidant activity was methanol solvent.

scholarly journals Phytochemical Analysis of Caesalpinia pulcherrima (L.) Sw. Leaf Extract Using GC-MS Analysis

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Saravanan P.
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Folk Medicine ◽  
Phytochemical Analysis ◽  
Pharmacological Activities ◽  
Malarial Infection ◽  
Ms Analysis ◽  
Soxhlet Apparatus

Most of the worlds populations residing in developing countries depend on alternative medicine and use of plant ingredients. Caesalpinia pulcherrima (Fabaceae) is used in folk medicine for the treatment of various diseases including asthma, bronchitis, cholera, diarrhea, dysentery and malarial infection. The aim of the study to identified bioactive compounds presence in the chloroform leaf extract of Caesalpinia pulcherrima (L.) Sw. using Gas Chromatography and Mass Spectrometry (GC-MS) analysis. The leaf extraction was done by Soxhlet apparatus method. The GC-MS analysis has shown the presence of thirteen compounds in the chloroform leaf extract of Caesalpinia pulcherrima (L.) Sw. The identified bioactive compounds from this plant have been known to possess pharmacological activities.

scholarly journals Antimalarial, Antioxidant Activities and Chemoprofile of Sansevieria liberica Gerome and Labroy (Agavaceae) Leaf Extract

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Ifeoma Celestina Orabueze ◽  
Sarah Chiamaka Uzor ◽  
Bou Ndiaye ◽  
Desire Uba ◽  
Duncan Azubuike. Ota ◽  
...  
Keyword(s):  
Vitamin C ◽  
High Performance ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Reducing Power ◽  
In Vitro Models ◽  
Phytochemical Analysis ◽  
Perennial Plant

Background. Sansevieria liberica Gerome and Labroy (Agavaceae) is a religious and ornamental perennial plant with highly valued medicinal usage in Nigeria. Sansevieria liberica is used in the management of malarial fever. The ease of development of resistance to available antimalarial drugs has resulted in increased clinical failure and mortality. The study investigated the antimalarial effects of Sansevieria liberica (SL) leaf extract in mice infected with Plasmodium berghei. Materials and Methods. The ability of SL leaf extract to suppress the growth of malaria parasites in early (suppressive) and established (curative) infections was established using animal models. The mean survival time (MST) was determined. The antioxidant potential was established using two standard in vitro models. High-performance liquid chromatography (HPLC) and phytochemical analysis methods were used to create a chemoprofile fingerprint of SL hydroethanolic leaf extract. Results. At 200, 400, and 400·mg kg−1, SL produced 68.71, 70.74, and 75.09% parasite suppression in the suppressive model while the curative model gave a percentage of cure of 71.09, 72.60, and 62.09, respectively. The animals lived longer compared to both negative and positive controls but were not fully protected. The IC50 values of SL and vitamin C were calculated to be 3.599 µg mL−1 and 3.08 µg mL−1, respectively. The reducing power of vitamin C was significantly ( P < 0.05 ) higher than that of SL extract. Some flavonoids were established as possible marker compounds for SL leaf extract. Discussion and Conclusions. The antimalarial assay results demonstrated that the use of SL in folk medicine may have scientific support.

scholarly journals Phytochemical Screening and Anti-Inflammatory Studies of Tapinanthus globiferus (A. Rich) Teigh. Leaves Three Extracts

2019 ◽  
Vol 25 (2) ◽  
pp. 124-131 ◽  
Author(s):  
Celestine Jeremiah ◽  
Umar Adam Katsayal ◽  
Aliyu Nuhu ◽  
Sherifat Bola Anafi ◽  
Mustapha Adeojoh Ibrahim ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Traditional Use ◽  
Paw Oedema ◽  
Soxhlet Apparatus ◽  
Percentage Yield ◽  
Anti Inflammatory

Background: Tapinanthus globiferus (A. Rich) Teigh. (Loranthaceae) is an excellent medicinal plant in terms of efficacy and also traditionally used for the treatment of various diseases including inflammations, cancer, diabetes and strokes. This study was designed to assess the anti-inflammatory activity of the leaf extracts of T. globiferus in wistar rats and identify phytochemical constituents of the extracts responsible for the observed activity. Methods: Tapinanthus globiferus leaves was extracted with hexane, ethyl acetate and methanol in a soxhlet apparatus. The extracts were subjected to qualitative phytochemical analysis, toxicity and anti-inflammatory activity using carrageenan-induced paw oedema in wistar rats. Piroxicam (20 mg/kg) was used as reference standard. The data were analyzed by one-way analysis of variance with significant level set at p≤0.05. Results: The percentage yield from the gradient extraction of T. globiferus leaves showed methanol to be the highest and the chromatographic analysis visualized with specific reagents confirmed the presence of steroids/triterpenes, phenolic compounds and flavonoids in the leaf of T. globiferus. LD50 was above 2,000 mg/kg and no death was recorded. The hexane, ethyl acetate and methanol leaf extracts of T. globiferus at 250, 500 and 1,000 mg/kg produced a significant decrease in paw oedema (p≤0.05) with percentage inhibition at the first and third hour for hexane, ethyl acetate and methanol extract respectively. The methanol extracts recorded the highest inflammatory inhibition percentage. Conclusion: These finding revealed that the leaf of T. globiferus has anti-inflammatory activity and this justified its traditional use in the treatment of inflammation.

Phytochemical Analysis and Antinociceptive Properties of Hydroalcoholic Extracts of Aleurites moluccanus Bark

Planta Medica ◽  
2021 ◽  
Author(s):  
Márcia Maria de Souza ◽  
Luiz G. R. D. Chagas ◽  
Ana Elisa Gonçalves ◽  
Marcelo Tomczak ◽  
Simone Reichert ◽  
...  
Keyword(s):  
Folk Medicine ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Solvent Ratio ◽  
Inflammatory Processes ◽  
Anti Inflammatory ◽  
Hydroethanolic Extract ◽  
Extracting Solvent ◽  
Positive Results ◽  
Necrosis Factor

Abstract Aleurites moluccanus is used in folk medicine to treat many diseases including pain and inflammatory processes in general. Considering the potential of the leaf extract, evidenced in a previous study, the present study investigates the antinociceptive and anti-inflammatory properties of the hydroethanolic extract of A. moluccanus bark and isolated compounds in animal models of pain. The antinociceptive and anti-inflammatory activities of A. moluccanus bark were evaluated through hyperalgesia induced by carrageenan, PGE2, cytokines, bradykinin, epinephrine, Freundʼs complete adjuvant, and lipopolysaccharide. Five compounds were isolated from the dichloromethane bark extract: acetyl aleuritolic acid, atraric acid, spruceanol, (5β,10α)-12-hydroxy-13-methoxy-8,11,13-podocarpatrien-3-one and sonderianol. To optimize the extraction conditions, ethanol 50, 70, and 90°GL were used as extracting solvent, in a 1 : 20 (w/v) drug : solvent ratio, under stirring at room temperature for 4 h. The extracts were named AMC50, AMC70, and AMC90, respectively. These extracts were administered to mice (250 mg/kg, p. o.) with reduced mechanical hyperalgesia activity in the carrageenan test. Of these, AMC90 showed the best results. Pure (5β,10α)-12-hydroxy-13-methoxy-8,11,13-podocarpatrien-3-one showed a beneficial effect for up to 48 hours after the administration of carrageenan, while acetyl aleuritolic acid was effective only in the first hour. AMC90 was able to reverse the analgesia induced only by prostaglandin E2 and tumor necrosis factor. We also induced hyperalgesia using the lipopolysaccharide and Freundʼs complete adjuvant models, with positive results. These results support the antinociceptive and anti-inflammatory activity of A. moluccanus bark extract. The observed effects are partly due to the presence of acetyl aleuritolic acid, atraric acid, and (5β,10α)-12-hydroxy-13-methoxy-8,11,13-podocarpatrien-3-one.

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