scholarly journals Quantification of Caffeine and Chlorogenic Acid in Green and Roasted Coffee Samples Using HPLC-DAD and Evaluation of the Effect of Degree of Roasting on Their Levels

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7502
Author(s):  
Shady Awwad ◽  
Reem Issa ◽  
Lilian Alnsour ◽  
Dima Albals ◽  
Idrees Al-Momani
Keyword(s):  
Chlorogenic Acid ◽  
Acid Content ◽  
High Performance ◽  
Hot Water ◽  
Array Detector ◽  
Phase System ◽  
Roasted Coffee ◽  
Coffee Beans ◽  
Different Origins ◽  
Antioxidant Effects

Chlorogenic acid and caffeine are among the important components in coffee beans, determining the taste and aroma. In addition, phenols and antioxidants content possess vital health values. The main aim of this study is to determine the levels of caffeine and chlorogenic acid in several coffee samples of different origins and degrees of roasting. The coffee samples were extracted using hot water. The levels of caffeine and chlorogenic acid were quantified using high-performance liquid chromatography (HPLC) equipped with a diode array detector, a reverse phase system, and an ODS column (C18). Total phenol and antioxidant contents were previously determined for the same samples. The results showed that the highest content of caffeine was found in the medium roasted coffee (203.63 mg/L), and the highest content of chlorogenic acid content was found in the green coffee (543.23 mg/L). The results demonstrated a negative correlation between the chlorogenic acid levels with the degree of roasting, while it showed a positive correlation between the caffeine levels with the degree of roasting till a certain point where the levels dropped in the dark roasted coffee. The origin of coffee samples did not show any effect on any of the measured variables. Antioxidant effects of coffee samples were largely determined by chlorogenic acid content.

scholarly journals Influence of Altitude on Caffeine, 5-Caffeoylquinic Acid, and Nicotinic Acid Contents of Arabica Coffee Varieties

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Bealu Girma ◽  
Abera Gure ◽  
Feyisa Wedajo
Keyword(s):  
Nicotinic Acid ◽  
High Performance ◽  
Array Detector ◽  
Roasted Coffee ◽  
Arabica Coffee ◽  
Caffeoylquinic Acid ◽  
Coffee Beans ◽  
Roasting Process ◽  
Target Analytes ◽  
Roasted Coffee Beans

The influence of altitude on caffeine, 5-caffeoylquinic acid (5-CQA), and nicotinic acid contents of Arabica coffee varieties grown in Southwest Ethiopia was investigated. High-performance liquid chromatography with diode array detector (HPLC-DAD) was used for the determination of the target analytes. Coffee samples were collected from four coffee varieties, named as 74112, 7454, 7440, and 74110, which are cultivated in high, mid, and low altitudes in the study area. The findings of the study showed that the contents of caffeine and 5-CQA in both raw and roasted coffee beans decrease as the growing altitude increases and, thus, for all varieties, their highest concentrations were recorded in lowland coffee beans. Nevertheless, the contents of nicotinic acid increase as the altitude rises and, thus, the highest nicotinic acid content was recorded in highland coffee samples. Besides, after roasting, the contents of caffeine were increased, whereas the contents of 5-CQA were lowered, indicating the possibility of its degradation during the roasting process. Both green and roasted coffee beans also contained relatively higher concentrations of nicotinic acids. Other than the growing altitudes, the contents of caffeine, 5-CQA, and nicotinic acid in coffee beans also vary with coffee varieties. Therefore, coffee varieties that are cultivated at various altitudes may have different biochemical compositions such as caffeine, CGAs, and nicotinic acid that could greatly influence the flavor, aroma, and stimulating attributes of coffee cup quality as well as dietary benefits.

scholarly journals In Vitro Culture of Rosmarinus officinalis L. in a Temporary Immersion System: Influence of Two Phytohormones on Plant Growth and Carnosol Production

2021 ◽  
Vol 14 (8) ◽  
pp. 747
Author(s):  
Eder Villegas Sánchez ◽  
Mariana Macías-Alonso ◽  
Soraya Osegueda Robles ◽  
Lisset Herrera-Isidrón ◽  
Hector Nuñez-Palenius ◽  
...  
Keyword(s):  
High Performance ◽  
Emerging Infectious Diseases ◽  
Rosmarinus Officinalis ◽  
Wild Plant ◽  
Array Detector ◽  
Phase System ◽  
Naphthaleneacetic Acid ◽  
Temporary Immersion System ◽  
Temporary Immersion

Emerging infectious diseases have become a major global problem with public health and economic consequences. It is an urgent need to develop new anti-infective therapies. The natural diterpene carnosol exhibit a wide variety of interesting antibacterial and antiviral properties, and it is considered a theoretical inhibitor of COVID-19 Mpro. However, this compound is present in the family Lamiaceae in low quantities. To obtain carnosol in concentrations high enough to develop pharmacological studies, we evaluated the efficiency of a micropropagation protocol of Rosmarinus officinalis using a solid medium and a temporary immersion system (TIS), as well as the effect of 6-benzylaminopurine (6-BAP) and α-naphthaleneacetic acid (NAA) on the growth of shoots. Moreover, we developed and validated an analytical method to quantify carnosol using the H-point standard additions method in the high-performance liquid chromatography diode array detector (HPLC-DAD). After 30 days of culture, TIS produced the maximum number of shoots per explant (24.33 ± 1.15) on a liquid medium supplemented with 6-BAP at 5.0 mg L−1. Next, we also evaluated the effect of immersion time and frequency for TIS. After 72 days of culture, the best results were obtained with an immersion cycle of 1 min every 12 h, yielding 170.33 ± 29.40 shoots. The quantification of carnosol on the samples was performed at a flow rate of 1.2 mL min−1 using binary isocratic mobile phase system 60:40 (v/v) 10 mM formic acid (pH 3.0) (A) and acetonitrile (B) on a reverse-phase column. The content of carnosol in the in vitro cultures was around 8-fold higher than in the wild plant. The present study represents an efficient alternative method to obtain carnosol for its pre-clinical and clinical development.

scholarly journals Enumerate chlorogenic acid in different forms of coffee by simple high performance thin layer chromatographic method

2021 ◽  
Vol 9 (2) ◽  
pp. 120-126
Author(s):  
Swati Bhattacharyya ◽  
Alok Kumar Hazra ◽  
BantiChakraborty ◽  
Tapas Kumar Sur ◽  
AvijitSaha
Keyword(s):  
Chlorogenic Acid ◽  
High Performance ◽  
Coffee Consumption ◽  
Health Benefit ◽  
Cost Effective ◽  
Biological Properties ◽  
Chromatographic Technique ◽  
Indian Market ◽  
Roasted Coffee ◽  
Phenolic Constituent

The health benefit of coffee consumption mainly depends on chlorogenic acid (CGA), one of its major active phenolic constituent. CGA have several biological properties like anticancer, antihypertensive, hepatoprotective, neuroprotective, renoprotective, antimicrobial etc. It is assumed that different forms of coffee contains different amount of CGA. In this regards, we developed and validated a simple and cost effective chromatographic technique to identify and quantify CGA in green coffee, roasted coffee and branded instant coffee available in Indian market.

Composition of amino acids and fatty acids on Luwak coffee processing

Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 60-64
Author(s):  
Fitri ◽  
A. Laga ◽  
Z. Dwyana ◽  
A.B. Tawali
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Amino Acid ◽  
Acid Content ◽  
Green Bean ◽  
Total Amino Acid ◽  
Coffee Bean ◽  
Roasted Coffee ◽  
Coffee Beans

The processing carried out on coffee beans such as fermentation and roasting can affect the contents of amino and fatty acids of coffee beans. This study aimed to determine the amount of amino acid and fatty acid content in Luwak coffee bean during processing. The amino acids of coffee were analyzed using HPLC, while fatty acids of coffee were analyzed using GC-MS. The results showed a change in total amino acid content in raw coffee bean (3.04%), green bean coffee (6.93%), and roasted coffee (6.83%). The total fatty acid of raw coffee bean (1199.86 mg/100 g), green bean coffee (3147.56 mg/100 g), and roasted coffee (6282.4 mg/100 g) also experienced significant changes

scholarly journals Determination of Triglycidyl Isocyanurate in Workplace Air

2019 ◽  
Vol 16 (22) ◽  
pp. 4455
Author(s):  
Anna Jeżewska ◽  
Joanna Kowalska
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
Crosslinking Agent ◽  
Measurement Range ◽  
Array Detector ◽  
Phase System ◽  
Limit Of Quantification ◽  
Workplace Air ◽  
Air Sample ◽  
Triglycidyl Isocyanurate

Triglycidyl isocyanurate (TGIC) is a white solid in powder or granular form. TGIC does not occur naturally in the environment. It is intentionally manufactured and used as a crosslinking agent or hardener to produce polyester powder coatings. TGIC may cause genetic defects. This article presents the method of TGIC determination in workplace air using high-performance liquid chromatography (HPLC) with a diode-array detector (DAD). The method is based on the collection of TGIC present in the air on a polypropylene filter, extraction with acetonitrile, and chromatographic analysis of the solution obtained in this way. The determination was carried out in the reverse-phase system (mobile phase: acetonitrile: water) using an Ultra C18 column. The measurement range is 2 to 40 µg/m3 for a 720 liters air sample. Limit of detection (LOD) is 23 ng/m3 and limit of quantification (LOQ): 70 ng/m3. The method can be used for assessing occupational exposure to TGIC and associated risk to workers’ health.

scholarly journals Physical Stability and HPLC Analysis of Indian Kudzu (Pueraria tuberosaLinn.) Fortified Milk

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Subha Rastogi ◽  
Antariksha Katara ◽  
Madan M. Pandey ◽  
Sumit Arora ◽  
R. R. B. Singh ◽  
...  
Keyword(s):  
High Performance ◽  
Functional Foods ◽  
Physical Stability ◽  
Health Benefits ◽  
Health Benefit ◽  
Hot Water ◽  
Array Detector ◽  
Experimental Conditions ◽  
Fortified Milk ◽  
Important Medicinal Plant

Functional foods provide health benefit beyond basic nutrition. Functional foods fortified with plant ingredients are well known. Ayurveda (Indian System of Medicine) has found several ways in which the medicinal benefits of herbs can be conveyed via certain foods as carriers. Milk is one such carrier which has been effectively used to deliver phytochemicals for targeted health benefits. Indian Kudzu orPueraria tuberosaLinn. (Fabaceae) is an important medicinal plant of Ayurveda, and experiments suggest that it enhances the health benefits of milk when taken with milk as a carrier. Different milk combinations withP. tuberosawere prepared by homogenizing pasteurized toned milk with its ethanolic and hot water extracts and their stability with reference to pH and coagulation was studied over a period of 15 days. The combinations were also analyzed for puerarin, the major isoflavone C-glucoside present inP. tuberosa, through high-performance liquid chromatography using photo diode array detector. It was observed that there was no precipitate formation and the pH also did not change during the study period indicating their physical stability under the experimental conditions. Also there was no significant change in the content of puerarin during the study period, thereby indicating the chemical stability of the samples. These studies will be useful for developing milk nutraceuticals fortified with Indian Kudzu which has the potential to be included as an ingredient in health and functional foods.

Chlorogenic acid (CGA) determination in roasted coffee beans by Near Infrared (NIR) spectroscopy

2014 ◽  
Vol 7 (4) ◽  
pp. 139-142 ◽  
Author(s):  
Jiajia Shan ◽  
Tetsuhito Suzuki ◽  
Diding Suhandy ◽  
Yuichi Ogawa ◽  
Naoshi Kondo
Keyword(s):  
Chlorogenic Acid ◽  
Near Infrared ◽  
Nir Spectroscopy ◽  
Roasted Coffee ◽  
Coffee Beans ◽  
Roasted Coffee Beans

scholarly journals A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough Syrup

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Ayman Khdair ◽  
Mohammad K. Mohammad ◽  
Khaled Tawaha ◽  
Eman Al-Hamarsheh ◽  
Hatim S. AlKhatib ◽  
...  
Keyword(s):  
High Performance ◽  
Orthophosphoric Acid ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Hplc Method ◽  
Array Detector ◽  
Phase System ◽  
Intermediate Precision ◽  
Rp Hplc ◽  
Cough Syrup

A simple reversed phase high-performance liquid chromatographic (RP-HPLC) method coupled with a photodiode array detector (PAD) has been developed and validated for the analysis of hederacoside C, the marker of ivy plant, in Ivy-Thyme cough syrup. Separation of hederacoside C was achieved using a Phenomenex-Gemini C18 column isothermally at C. A mobile phase system constituted of solvent A (water: acetonitrile: orthophosphoric acid (85%), 860 : 140 : 2 v/v) and solvent B (acetonitrile: orthophosphoric acid (85%), 998 : 2 v/v) was used, at gradient conditions, at a flow rate of 1.5 mL/min. Analysis was performed using UV-detection (205 nm). The method was linear over the range (0.03–0.15) mg/mL of hederacoside C (). Repeatability and intermediate precision were acceptable (RSD %). Limits of detection (LOD) and quantitation (LOQ) were 0.011 and 0.032 mg/mL, respectively. Percentage recovery was found to lie between 99.69% and 100.90% (RSD %). The method was also proved to be specific (peak-purity ).

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