One-Pot Synthesis of SiO2@Ag Mesoporous Nanoparticle Coating for Inhibition of Escherichia coli Bacteria on Various Surfaces

Silver nanoparticles (Ag NPs) as antibacterial agents are of considerable interest owing to their simplicity, high surface area to volume ratio, and efficient oligodynamic properties. Hence, we investigated the synthesis of silica-supported Ag NPs (SiO2@Ag) as an effective antibacterial agent by using a wet-impregnation method. The formation of SiO2@Ag with Ag NP (5–15 nm diameter) on the silica particle (100–130 nm diameter) was confirmed with transmission electron microscopy (TEM). The study on antibacterial activity was performed in a liquid culture to determine the minimum inhibitory concentration (MIC) against Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis) bacteria. Both bacteria are chosen to understand difference in the effect of Ag NPs against Gram-negative (E. coli) and Gram-positive (B. subtilis) bacteria. SiO2@Ag mesoporous nanoparticles had excellent antibacterial activity against E. coli bacteria and fully restricted the bacterial growth when the material concentration was increased up to 1.00 mg/mL. In addition, the obtained material had good adhesion to both steel and polyethylene substrates and exhibited a high inhibition effect against E. coli bacteria.

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The Antibacterial Activity Of Several Sponges From The Waters Of Tasik Ria Against Escherichia coli And Staphylococcus aureus bacteria

JURNAL ILMIAH PLATAX ◽

10.35800/jip.7.1.2019.21435 ◽

2018 ◽

Vol 7 (1) ◽

pp. 1

Author(s):

Abrianto A. O. Rompis ◽

Fitje Losung ◽

Deiske A. Sumilat ◽

Agung B. Windarto ◽

Stenly Wullur ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Natural Compounds ◽

E Coli ◽

Escherichia Coli Bacteria ◽

And Staphylococcus Aureus ◽

Positive Results ◽

Sponge Extract

The sponge is one of the sea organisms that has a prospect as a source of natural compounds including peptides, steroids, asetogenin, terpenoids, alkaloids, cyclic halide and nitrogen. This research was directed to obtain several species of sponges from the waters of Tasik Ria as well as testing the antibacterial activity of extracts from some of the sponge against the bacteria Escherichia coli and Staphylococcus aureus. From the identification, seven species of sponges were found, which consists of: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Cribochalina sp., Hyrtios sp., and Lendenfeldia sp. The tests of antibacterial activity of the extracts from these sponges against test bacteria E. coli and S. aureus showed some positive results. Extract from Axinosa sp. sponge(16 mm) showed the strongest antibacterial activity on Escherichia coli bacteria. Followed by Hyrtios sp. extract (13.5 mm), Aaptos sp. extract (13 mm), Lendenfeldia sp. extract (13 mm) and Cribochalinai sp. extract(10.5 mm). While the the tests on Staphylococcus aureus bacteria showed that the strongest antibacterial activity was found from Axinosa sp. sponge extract (16.5 mm), followed by the extract from Aaptos sp. (15 mm), Lendenfeldia sp. extract (14.5 mm), Hyrtios sp. extract(13.5 mm) and Cribochalina sp. extract (11 mm).Keywords: Sponge, antibacterial, Escherichia coli, Staphylococcus aureus ABSTRAK Spons merupakan salah satu biota laut yang sangat prospektif sebagai sumber senyawa bahan-bahan alami antara lain peptide, terpenoid, steroid, asetogenin, alkaloid, halide siklik dan senyawa nitrogen. Penelitian ini diarahkan untuk mendapatkan beberapa spesies spons dari perairan Tasik Ria serta menguji aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri Escherichia coli dan bakteri Staphylococcus aureus. Hasil identifikasi spons ditemukan sebanyak tujuh spesies yang terdiri dari: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Hyrtios sp., Cribochalina sp. dan Lendenfeldia sp.. Aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri uji E. coli dan S. aureus terdapat diameter zona hambat bervariasi yaitu bakteri Escherichia coli menunjukkan aktivitas antibakteri ekstrak spons terkuat pada spons Axinosa sp (16 mm), disusul ekstrak spons Hyrtios sp. (13,5 mm), ekstrak spons Aaptos sp. (13 mm), ekstrak spons Lendenfeldia sp. (13 mm) dan ekstrak spons Cribochalinai sp. (10,5 mm). Sedangkan pada bakteri Staphylococcus aureus menunjukkan aktivitas antibakteri ekstrak spons terkuat yaitu: ekstrak spons Axinosa sp. (16,5 mm), disusul ekstrak spons Aaptos sp. (15 mm), ekstrak spons Lendenfeldia sp. (14,5 mm), ekstrak spons Hyrtios sp. (13,5 mm) dan ekstrak spons Cribochalina sp.(11mm).Kata Kunci : Spons, Antibakteri, Escherichia coli, Staphylococcus aureus

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Synergistic Antibacterial Activity of Silver-Loaded Graphene Oxide towards Staphylococcus Aureus and Escherichia Coli

Nanomaterials ◽

10.3390/nano10020366 ◽

2020 ◽

Vol 10 (2) ◽

pp. 366 ◽

Cited By ~ 8

Author(s):

Truong Vi ◽

Selvaraj Kumar ◽

Jong-Hwei Pang ◽

Yu-Kuo Liu ◽

Dave Chen ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bactericidal Effect ◽

Catalytic Performance ◽

Redox Activity ◽

Ag Nps ◽

E Coli ◽

Transmission Electron ◽

Physicochemical Effect

In this study, the physicochemical and surface properties of the GO–Ag composite promote a synergistic antibacterial effect towards both Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. Aureus) bacteria. GO–Ag NPs have a better bactericidal effect on E. coli (73%) and S. Aureus (98.5%) than pristine samples (pure Ag or GO). Transmission electron microscopy (TEM) confirms that the GO layers folded entire bacteria by attaching to the membrane through functional groups, while the Ag NPs penetrated the inner cell, thus damaging the cell membrane and leading to cell death. Cyclic voltammetry (CV) tests showed significant redox activity in GO–Ag NPs, enabling good catalytic performance towards H2O2 reduction. Strong reactive oxygen species (ROS) in GO–Ag NPs suggests that ROS might be associated with bactericidal activity. Therefore, the synergy between the physicochemical effect and ROS production of this material is proposed as the mechanism of its antibacterial activity.

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UJI EFEK ANTIBAKTERI JAMUR ENDOFIT PADA TUMBUHAN SEREH (Cymbopogon citratus) PADA BAKTERI UJI Staphylococcus aureus DAN Escherichia coli

PHARMACON ◽

10.35799/pha.8.2019.29395 ◽

2019 ◽

Vol 8 (3) ◽

pp. 705

Author(s):

Fathia Falugah ◽

Jimmy Posangi ◽

Paulina V. Y. Yamlean

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Endophytic Fungi ◽

Pathogenic Bacteria ◽

Antibacterial Effect ◽

Cymbopogon Citratus ◽

E Coli ◽

Gram Positive Bacteria ◽

Escherichia Coli Bacteria

ABSTRACT Endophytic fungi are fungi that live in plant tissues and do not harm these plants. Endophytic fungi can produce compounds that have the potential as antibacterial. This study aimed to examine the antibacterial effects of endophytic fungi isolated from the lemongrass (Cymbopogon citratus) against Staphylococcus aureus and Escherichia coli bacteria. Lemongrass is used to inhibit or kill pathogenic bacteria because it contains essential oils that function as an antifungal and antibacterial against several pathogenic bacteria such as Staphylococcus aureus and Escherichia coli. The laboratory experiment method tested the antibacterial activity of endophytic fungi isolates obtained from lemongrass (Cymbopogon citratus) against Staphylococcus aureus and Escherichia coli using hole / well method. From the results of the study, four endophytic fungi were isolated from the roots and leaves of lemongrass (Cymbopogon citratus). Antibacterial results showed that endophytic fungi extract isolated from lemongrass plants was more effective in inhibiting Staphylococcus aureus compared to Escherichia coli bacteria. In Staphylococcus aureus bacteria the average inhibitory value is 5-9 mm while in Escherichia coli bacteria the average inhibitory value is only 3 mm. Endophytic fungi from lemongrass roots have a better antibacterial effect compared to endophytic fungi from lemongrass leaves. Conclusion, endophytic fungi isolated from the roots and leaves of lemongrass (Cymbopogon citratus) have an antibacterial effect on the growth of S. aureus bacteria and are not effective against E. coli bacteria. This endophytic fungus isolate is more active against Gram positive (+) bacteria and less active against Gram negative (-) bacteria. Keywords: Lemongrass plants, Endophytic Fungi, Antibacterial Activity, Staphylococcus aureus, Escherichia coli. ABSTRAK Jamur endofit ialah jamur yang hidup di dalam jaringan tumbuhan dan tidak membahayakan tumbuhan tersebut. Jamur endofit dapat menghasilkan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk menguji efek antibakteri jamur endofit yang diisolasi dari akar,batang dan daun tumbuhan sereh (Cymbopogon citratus) terhadap bakteri Staphylococcus aureus dan Escherichia coli. Sereh digunakan untuk menghambat atau membunuh bakteri patogen karena mengandung minyak atsiri yang berfungsi sebagai antijamur dan antibakteri terhadap beberapa bakteri patogen seperti Staphylococcus aureus dan Escherichia coli. Metode penelitian secara eksperimen laboratorium menguji aktivitas antibakteri dari isolat jamur endofit yang diperoleh dari tumbuhan sereh (Cymbopogon citratus) pada bakteri Staphylococcus Aureus dan Escherichia Coli menggunakan metode lubang/sumuran. Dari hasil penelitan diperoleh empat jenis jamur endofit yang diisolasi dari akar dan daun tumbuhan sereh (Cymbopogon citratus). Hasil penelitian antibakteri menunjukkan bahwa ekstrak jamur endofit yang diisolasi dari tumbuhan sereh lebih efektif menghambat bakteri Staphylococcus aureus dibandingkan dengan bakteri Escherichia coli. Pada bakteri Staphylococcus aureus nilai rata-rata daya hambat yaitu 5-9 mm sedangkan pada bakteri Escherichia coli nilai rata-rata daya hambat yaitu hanya 3 mm. Jamur endofit dari akar sereh memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit dari daun sereh. Kesimpulan, Jamur endofit yang diisolasi dari akar dan daun sereh (Cymbopogon citratus) memiliki efek antibakteri terhadap pertumbuhan bakteri S. aureus dan tidak efektiv terhadap bakteri E. coli. Isolat jamur endofit ini lebih aktif pada bakteri Gram positif (+) dan kurang aktif pada bakteri negatif (-). Kata Kunci : Tumbuhan Sereh, Jamur Endofit, Aktivitas Antibakteri, Staphylococcus aureus, Escherichia coli

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AKTIVITAS ANTIBAKTERI INFUSA DAUN MUDA MANGROVE Sonneratia alba KERING

MEDIA TEKNOLOGI HASIL PERIKANAN ◽

10.35800/mthp.7.2.2019.23613 ◽

2019 ◽

Vol 7 (2) ◽

pp. 52

Author(s):

Yayu Mukhmin Ibrahim ◽

Verly Dotulong ◽

Djuhria Wonggo ◽

Helen Jenny Lohoo ◽

Roike Iwan Montolalu ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Extraction Time ◽

Gram Positive ◽

Gram Negative ◽

E Coli ◽

Sample Concentration ◽

Sonneratia Alba ◽

Escherichia Coli Bacteria

Sonneratia alba mangroves are known to have bioactive compounds such as antibacterial. The purpose of this study was to determine the antibacterial activity of extract of S. alba dried mangrove leaves on Gram positive Staphylococcus aureus and Gram negative Escherichia coli bacteria. Extraction method by infusion, extraction time is 40 and 50 minutes. The extract obtained was then evaporated by the solvent above the water bath then the extracts were made concentrations of 5 and 10%. Antibacterial testing uses the modified Kirby-Bauer method. The highest yield is found in the treatment of 50 minutes infusion extraction time which is 15.6 ± 0.2%. The highest antibacterial activity against S. aureus was found in the treatment of 50 minutes extraction time both for 5% sample concentration of 7.0 mm (medium category) and at a sample concentration of 10% at 8.0 mm (medium category). While the highest antibacterial activity against E. coli bacteria was found in the treatment of 50 minutes extraction time both at 5% sample concentration of 8.0 (medium category) mm and at 10% sample concentration of 8.3 mm (medium category). From these results it can be seen that the S. alba extract of young mangrove leaf infusion has a broad spectrum antibacterial activity because it can inhibit both Gram positive S. aureus and Gram negative E. coli bacteria.Keyword: Mangrove, Sonneratia alba, infusion, rendemen, antibacterial. Mangrove Sonneratia alba diketahui memiliki senyawa bioaktif seperti antibakteri. Tujuan dari penelitian ini untuk mengetahui aktivitas antibakteri ekstrak Infusa daun muda mangrove S. alba kering terhadap bakteri Grampositif Staphylococcus aureus dan bakteri Gram negatif Escherichia coli. Metode ekstraksi dengan cara infusa, lama waktu ekstraksi yaitu40 dan 50 menit. Ekstrak yang didapatkan kemudian dievaporasi pelarutnya diatas penangas air kemudian hasil ekstrak dibuat konsentrasi 5 dan 10%. Pengujian antibakteri menggunakan metode Kirby-Bauer yang dimodifikasi. Rendemen tertinggi terdapat pada perlakuan lama ekstraksi infusa 50 menit yaitu sebesar 15,6±0,2 %. Aktivitas antibakteri tertinggi terhadap bakteri S. aureus terdapat pada perlakuan lama ekstraksi 50 menit baik untuk konsentrasi sampel 5% sebesar 7,0 mm (kategori sedang) dan pada konsentrasi sampel 10% sebesar 8,0 mm (kategori sedang). Sedangkan aktivitas antibakteri tertinggi terhadap bakteri E. coli terdapat pada perlakuan lama ekstraksi 50 menit baik pada konsentrasi sampel 5% sebesar 8,0 mm (kategori sedang) dan pada konsentrasi sampel 10% sebesar 8,3 mm (kategori sedang). Dari hasil ini dapat dilihat bahwa ekstrak infusa daun muda mangrove S. alba mempunyai aktivitas antibakteri dengan spektrum yang luas karena dapat menghambat baik bakteri Gram positif S. aureus maupun Gram negatif E. coli.Kata kunci: Mangrove, Sonneratia alba, infusa, rendemen, antibakteri.

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Hydroxyl-rich nanoporous carbon nanosheets synthesized by a one-pot method and their application in the in situ preparation of well-dispersed Ag nanoparticles

RSC Advances ◽

10.1039/c5ra18300f ◽

2015 ◽

Vol 5 (116) ◽

pp. 96062-96066 ◽

Cited By ~ 5

Author(s):

Xiaodi Guo ◽

Gaili Liu ◽

Shuang Yue ◽

Jing He ◽

Lianying Wang

Keyword(s):

Ag Nanoparticles ◽

High Surface ◽

High Surface Area ◽

Nanoporous Carbon ◽

Hydroxyl Groups ◽

Ag Nps ◽

One Pot ◽

Carbon Nanosheets ◽

In Situ Preparation

Nanoporous carbon nanosheets (CNSs) with high surface area have been synthesized by pyrolysis of organic–inorganic precursor. The CNSs with rich hydroxyl groups display remarkable reactivity and capability for in situ loading ultrafine Ag NPs.

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AKTIVITAS ANTIBAKTERI FRAKSI ALGA Turbinaria ornata (Turner) J. Agardh YANG DIPEROLEH DARI PERAIRAN TELUK MANADO

PHARMACON ◽

10.35799/pha.8.2019.29298 ◽

2019 ◽

Vol 8 (2) ◽

pp. 325

Author(s):

Nadia Adam ◽

Widya A. Lolo ◽

Sri Sudewi

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Raw Materials ◽

Diffusion Method ◽

Marine Biota ◽

Turbinaria Ornata ◽

E Coli ◽

Escherichia Coli Bacteria

ABSTRACT Marine biota such as algae has been developed to be used as a source of medicinal raw materials in the pharmaceutical industry. Extracs from Turbinaria ornata algae J. Agardh showed antibacterial activity. This study aims to determine the antibacterial activity of the Turbinaria ornata (Turner) J. Agardh fraction obtained from the waters of the Manado Bay against the growth of Staphylococcus aureus and Escherichia coli bacteria. The method used in this study is an experimental laboratory. Turbinaria ornata (Turner) J. Agardh was extracted by maceration method with ethanol 96% solvent followed by fractionation and antibacterial activity testing with diffusion method. Antibacterial activity was determined based on the results of the clear zone measurements formed around the disc paper. The results showed that the methanol at a concentration of 10%, 20%, 30%, fractions of 30% ethyl acetate, and fractions of 20% and 30% n-hexane, have antibacterial activity which is categorized strongly in both bacteria of S. aureus and E. coli, while ethyl acetate fractions with a concentration of 10% and 20% againts S.aureus is in the moderate category. Likewise the antibacterial activity of n-hexane fraction with a concentration of 10% againts E. coli was included in the medium category. Keywords: Turbinaria ornata (Turner) J. Agardh, antibacterial, Staphylococcus aureus, Escherichia coli ABSTRAK Biota laut seperti alga telah banyak dikembangkan untuk digunakan sebagai sumber bahan baku obat dalam industri farmasi. Ekstrak dari alga Turbinaria ornata (Turner) J. Agardh menunjukan adanya aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri fraksi Turbinaria ornata (Turner) J. Agardh yang diperoleh dari perairan Teluk Manado terhadap pertumbuhan bakteri Staphylococus aureus dan Escherichia coli. Metode yang digunakan dalam penelitian ini adalah eksperimental laboratorium. Turbinaria ornata (Turner) J. Agardh diekstraksi dengan metode maserasi dengan pelarut etanol 96% dilanjutkan dengan tahap fraksinasi dan pengujian aktivitas antibakteri dengan metode difusi. Aktivitas antibakteri ditentukan berdasarkan hasil pengukuran zona bening yang terbentuk disekitar kertas cakram. Hasil penelitian menunjukkan bahwa fraksi metanol pada konsentrasi 10%. 20% ,30%, fraksi etil asetat konsentrasi 30%, dan fraksi n-heksana pada konsentrasi 20%, 30% memiliki aktivitas antibakteri yang dikategorikan kuat pada kedua bakteri yaitu S. aureus dan E. coli, sedangkan fraksi etil asetat dengan konsentrasi 10% dan 20% pada bakteri S. aureus termasuk dalam kategori sedang. Begitu pula aktivitas antibakteri fraksi n-heksan dengan konsentrasi 10% terhadap E. coli termasuk dalam kategori sedang. Kata kunci : Turbinaria ornata (Turner) J. Agardh, antibakteri, Staphylococus aureus, Escherichia coli.

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Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria

JKPK (Jurnal Kimia dan Pendidikan Kimia) ◽

10.20961/jkpk.v3i3.22742 ◽

2018 ◽

Vol 3 (3) ◽

pp. 201 ◽

Cited By ~ 1

Author(s):

Suryadi Budi Utomo ◽

Mita Fujiyanti ◽

Warih Puji Lestari ◽

Sri Mulyani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Hexadecyltrimethylammonium Bromide ◽

E Coli ◽

Escherichia Coli Bacteria

This study aims to determine the antibacterial activity of C-4-methoxyphenylcalix-[4]resorcinarene modified by Hexadecyltrimethylammonium-Bromide (Resorcinarene-HDTMA-Br) against Staphylococcus aureus and Escherichia coli bacteria. The study was conducted by testing the inhibition zone diameter with paper disc diffusion method. Variations in the concentration of the sample used were 10%; 15%; 20%; 25%; and 30% b/v. In this study, Dimethyl sulfoxide (DMSO) (sampel solvent) was used as a negative control and Chloramphenicol as a positive control. It was found that Resorsinarene-HDTMA-Br had higher antibacterial activity than the unmodified C-4-methoxyphenylcalix[4]resorcinarene (CMFKR) against the S. aureus bacteria. Both CMFKR and Resorcinarene-HDTMA-Br are not active against E. coli.

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UJI AKTIVITAS ANTIBAKTERI JAMUR ENDOFIT PADA DAUN DAN BATANG TUMBUHAN KUMIS KUCING (Orthosiphon aristatus) TERHADAP BAKTERI Escherichia coli DAN staphylococcus aureus

PHARMACON ◽

10.35799/pha.9.2020.29273 ◽

2020 ◽

Vol 9 (2) ◽

pp. 211

Author(s):

Excel Pangouw ◽

Jimmy Posangi ◽

Widya Astuty Lolo ◽

Robert Bara

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Endophytic Fungi ◽

Antibacterial Effect ◽

E Coli ◽

Average Value ◽

Escherichia Coli Bacteria ◽

Cat’S Whiskers ◽

And Staphylococcus Aureus

ABSTRACTEndophytic fungi are fungi that live in plant tissues and do not harm the plants. Endophytic fungi can produce compounds that have the potential to be antibacterial. This study aims to examine the antibacterial activity of endophytic fungi isolated from the leaves and stems of the cat's whiskers plant (Orthosiphon aristatus) against Escherichia coli and Staphylococcus aureus bacteria. Cat's whiskers contain essential oils which are used as antibacterial against some pathogenic bacteria such as Staphylococcus aureus and Escherichia coli. The method used is experimentally in the laboratory to test the antibacterial activity of endophytic fungi isolates derived from cat's whiskers (Orthosiphon aristatus) on Escherichia coli and Staphylococcus aureus bacteria using the hole / well method. In the research results obtained four samples of endophytic fungi extract isolated from the leaves and stems of the cat's whiskers plant (Orthosiphon aristatus) . The results of antibacterial research showed that endophytic mushroom extract isolated from cat's whiskers were more effective in inhibiting Escherichia coli bacteria compared to Staphylococcus aureus. In Escherichia coli bacteria the average value of inhibition is 5 mm whereas in Staphylococcus aureus bacteria the average value of inhibition is only 2 mm. So it can be seen that the endophytic fungus from the leaves of cat's whiskers has a better antibacterial effect than the endophytic fungus from the stems of the cat's whiskets. Conclusion, etidophytic fungi isolated from leaves and stems of cat's whiskers (Orthosiphon aristatus) have an antibacterial effect on the growth of E. coli bacteria and are not effective against S. aureus bacteria.Keywords: Cat's whiskers (Orthasiphan aristatus), antibacterial activity, Escherichia coli and Staphylococcus aureus. ABSTRAKJamur endofit ialah jamur yang hidup di dalam jaringan tumbuhan dan tidak membahayakan tumbuhan tersebut. Jamur endofit dapat menghasilkan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk menguji aktivitas antibakteri jamur endofit yang diisolasi dari daun dan batang tumbuhan kumis kucing (Orthosiphon aristatus) terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Kumis kucing mengandung minyak atsiri yang digunakan sebagai antibakteri terhadap beberapa bakteri patogen seperti Staphylococcus aureus dan Escherichia coli. Metode yang dipakai yakni secara eksperimen di laboratorium untuk menguji adanya aktivitas antibakteri dari isolat jamur endofit yang diberasal dari tumbuhan kumis kucing (Orthosiphon aristatus) pada bakteri Escherichia coli dan Staphylococcus aureus dengan menggunakan metode lubang/sumuran. Pada hasil penelitan didapatkan empat sampel ekstrak jamur endofit yang diisolasi dari daun dan batang tumbuhan kumis kucing (Orthosiphon aristatus). Hasil penelitian antibakteri menunjukkan bahwa ekstrak jamur endofit yang diisolasi dari tumbuhan kumis kucing lebih efektif menghambat bakteri Escherichia coli dibandingkan dengan bakteri Staphylococcus aureus. Pada bakteri Escherichia coli nilai rata-rata daya hambat yaitu 5 mm sedangkan pada bakteri Staphylococcus aureus nilai rata-rata daya hambat yaitu hanya 2 mm. Sehingga dapat diketahui bahwa jamur endofit dari daun kumis kucing memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit dari batang kumis kucing. Kesimpulan, Jamur endofit yang diisolasi dari daun dan batang kumis kucing (Orthosiphon aristatus) memiliki efek antibakteri terhadap pertumbuhan bakteri E. coli dan tidak efektiv terhadap bakteri S. aureus.Kata Kunci: Tumbuhan kumis kucing (Orthosiphon aristatus), aktivitas antibakteri, Escherichia coli dan Staphylococcu saureus.

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Polymacron™ Enzyme Immunoassay System for Detection of Escherichia coli O157 Inoculated into Foods†

Journal of Food Protection ◽

10.4315/0362-028x-60.2.98 ◽

1997 ◽

Vol 60 (2) ◽

pp. 98-101 ◽

Cited By ~ 8

Author(s):

BURTON W. BLAIS ◽

RONALD A. BOOTH ◽

LUCILLE PHILLIPPE ◽

SITHIAN PANDIAN ◽

HIROSHI YAMAZAKI

Keyword(s):

Escherichia Coli ◽

Enzyme Immunoassay ◽

High Surface ◽

High Surface Area ◽

Polyester Fabric ◽

Sodium Cholate ◽

Escherichia Coli O157 ◽

Dot Blot ◽

E Coli ◽

Pure Cultures

A rapid and simple enzyme immunoassay system was developed for detection of the food-borne pathogen Escherichia coli O157. This system is based on the use of anti-E. coli O157 antibody-coated Polymacron™, an inexpensive macroporous polyester fabric, as a high-surface-area immunoadsorbent for the rapid capture and subsequent immunoenzymatic detection of E. coli O157 antigens extracted from test samples by heating at 100°C for 10 min in the presence of sodium cholate. A dot blot format was used which facilitated the assay of multiple samples. The method was specific for all strains tested bearing the O157 and related antigens (e.g., group N Salmonella), giving positive reactions in the assay of pure cultures of 29 E. coli O157:H7 strains, one E. coli O157:H12, one E. coli O157:NM (nonmotile) and one Salmonella urbana strain, but not in the assay of a variety of other gram-negative and gram-positive bacteria. The method permitted the detection of ca. 400 E. coli O157:H7 CFU in a 5-μl spot applied to Polymacron™, and of as few as 0.4 CFU of E. coli O157:147 cells per g inoculated into ground beef samples, which were assayed after overnight enrichment.

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Antibacterial Activities of Seaweed (Eucheuma cottonii ) Extract Against Escherichia coli Bacteria

BERKALA SAINSTEK ◽

10.19184/bst.v9i1.18184 ◽

2021 ◽

Vol 9 (1) ◽

pp. 6

Author(s):

Sukmawati Sukmawati

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Positive Control ◽

Negative Control ◽

Seaweed Extract ◽

E Coli ◽

Extract Solution ◽

Activity Test ◽

Escherichia Coli Bacteria ◽

Eucheuma Cottonii

Indonesia is a maritime country that has more than 70% of its territory dominated by oceans and is a marine area that has abundant natural resources, one of which is Eucheuma cottonii. The content of compounds by E. cottonii has many benefits, the compound content of E.cottonii is a phenolic compound (flavonoids). Flavonoids found in seaweed E. cottonii are secondary metabolite compounds that can produce antibacterial activity. The purpose of this study was to determine the antibacterial activity of seaweed extract (E. cottonii) against Escherichia coli bacteria. The method that has been used in this research is descriptive method, including extraction, maceration, antibacterial activity test. The results of this study stated that the antibacterial activity of E. cottonii seaweed extract was able to inhibit the growth of E. coli bacteria. The highest antibacterial activity was found at a concentration of 50% ppm dry extract solution with a methanol solvent of 3.3 mm while the lowest antibacterial activity was found at a concentration of 5% ppm wet extract solution with a methanol solvent whose inhibitory activity value was 0.1 mm. Furthermore, the antibacterial activity test for positive control showed a strong inhibitory value of 7.8 mm, while the negative control did not indicate any inhibitory value against E. coli bacteria.

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