scholarly journals DNA Damage, n-3 Long-Chain PUFA Levels and Proteomic Profile in Brazilian Children and Adolescents

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2483
Author(s):  
Tamiris Barros ◽  
Vinicius Venancio ◽  
Lívia Hernandes ◽  
Lusania Antunes ◽  
Elaine Hillesheim ◽  
...  
Keyword(s):  
Dna Damage ◽  
Children And Adolescents ◽  
Protective Role ◽  
Blood Levels ◽  
Healthy Children ◽  
Chain Pufa ◽  
Inflammatory Proteins ◽  
Low Levels ◽  
Cluster 2 ◽  
Long Chain

Fatty acids play a significant role in maintaining cellular and DNA protection and we previously found an inverse relationship between blood levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and DNA damage. The aim of this study was to explore differences in proteomic profiles, for 117 pro-inflammatory proteins, in two previously defined groups of individuals with different DNA damage and EPA and DHA levels. Healthy children and adolescents (n = 140) aged 9 to 13 years old in an urban area of Brazil were divided by k-means cluster test into two clusters of DNA damage (tail intensity) using the comet assay (cluster 1 = 5.9% ± 1.2 and cluster 2 = 13.8% ± 3.1) in our previous study. The cluster with higher DNA damage and lower levels of DHA (6.2 ± 1.6 mg/dL; 5.4 ± 1.3 mg/dL, p = 0.003) and EPA (0.6 ± 0.2 mg/dL; 0.5 ± 0.1 mg/dL, p < 0.001) presented increased expression of the proteins CDK8–CCNC, PIK3CA–PIK3R1, KYNU, and PRKCB, which are involved in pro-inflammatory pathways. Our findings support the hypothesis that low levels of n-3 long-chain PUFA may have a less protective role against DNA damage through expression of pro-inflammatory proteins, such as CDK8–CCNC, PIK3CA–PIK3R1, KYNU, and PRKCB.

scholarly journals Cross-sectional association between serum concentrations ofn-3 long-chain PUFA and depressive symptoms: results in Japanese community dwellers

2015 ◽  
Vol 115 (4) ◽  
pp. 672-680 ◽  
Author(s):  
Chika Horikawa ◽  
Rei Otsuka ◽  
Yuki Kato ◽  
Yukiko Nishita ◽  
Chikako Tange ◽  
...  
Keyword(s):  
Depressive Symptoms ◽  
Multiple Logistic Regression Analysis ◽  
Depression Scale ◽  
Serum Levels ◽  
Blood Levels ◽  
Serum Concentrations ◽  
Cross Sectional ◽  
Chain Pufa ◽  
Long Chain ◽  
Community Dwellers

AbstractThe effect ofn-3 long-chain PUFA (n-3 LCPUFA) on depression in healthy subjects is unclear, and most of the previous studies have focused on populations eating Western diets with lower fish intake. The present study investigated the association between blood levels ofn-3 LCPUFA and depressive symptoms in Japanese community dwellers with highern-3 LCPUFA blood levels. A cross-sectional study was conducted from 2006 to 2008, including 1050 men and 1073 women aged 40 years or older from the National Institute for Longevity Sciences – the Longitudinal Study of Aging. The Center for Epidemiologic Studies Depression Scale (CES-D) was used to assess depressive symptoms. Multiple logistic regression analysis was performed to estimate the OR and 95 % CI for a CES-D score ≥16. Serum concentrations ofn-3 PUFA, but notn-6 PUFA, were inversely associated with depressive symptoms. Compared with the lowest quintile, the adjusted OR for serum EPA at the fourth and fifth quintiles were 0·55 (95 % CI 0·35, 0·85) and 0·64 (95 % CI 0·42, 0·98), respectively, and at the fifth quintile for DHA it was 0·58 (95 % CI 0·37, 0·92), for the presence of depressive symptoms (Pfor trend=0·013 and 0·011, respectively). Serum levels of EPA and DHA were inversely associated with depressive symptoms in Japanese community dwellers with higher blood levels ofn-3 LCPUFA, suggesting thatn-3 LCPUFA intakes corresponding to higher levels in a Japanese population may have implications for a lower prevalence of depression.

DNA damage is inversely associated to blood levels of DHA and EPA fatty acids in Brazilian children and adolescents

2020 ◽  
Vol 11 (6) ◽  
pp. 5115-5121
Author(s):  
Tamiris Trevisan de Barros ◽  
Vinicius de Paula Venâncio ◽  
Lívia Cristina Hernandes ◽  
Lusânia Maria Greggi Antunes ◽  
Elaine Hillesheim ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dna Damage ◽  
Docosahexaenoic Acid ◽  
Eicosapentaenoic Acid ◽  
Children And Adolescents ◽  
Beta Carotene ◽  
Blood Levels ◽  
Brazilian Children

This study aimed to investigate the association between DNA damage and blood levels of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), retinol, beta-carotene and riboflavin in Brazilian children and adolescents.

Normal percentile curves for left atrial size in healthy children and adolescents

2019 ◽  
Vol 36 (4) ◽  
pp. 770-782 ◽  
Author(s):  
Alejandro Díaz ◽  
Yanina Zócalo ◽  
Daniel Bia
Keyword(s):  
Children And Adolescents ◽  
Left Atrial ◽  
Healthy Children ◽  
Left Atrial Size ◽  
Percentile Curves ◽  
Atrial Size

scholarly journals Folate and Cobalamin Serum Levels in Healthy Children and Adolescents and Their Association with Age, Sex, BMI and Socioeconomic Status

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 546
Author(s):  
Paulina Kreusler ◽  
Mandy Vogel ◽  
Anja Willenberg ◽  
Ronny Baber ◽  
Yvonne Dietz ◽  
...  
Keyword(s):  
Socioeconomic Status ◽  
Children And Adolescents ◽  
Serum Levels ◽  
Population Based ◽  
Research Centre ◽  
Healthy Children ◽  
Serum Samples ◽  
Lms Method ◽  
Healthy Participants ◽  
Age And Sex

This study proposes age- and sex-specific percentiles for serum cobalamin and folate, and analyzes the effects of sex, age, body mass index (BMI), and socioeconomic status (SES) on cobalamin and folate concentrations in healthy children and adolescents. In total, 4478 serum samples provided by healthy participants (2 months–18.0 years) in the LIFE (Leipzig Research Centre for Civilization Diseases) Child population-based cohort study between 2011 and 2015 were analyzed by electrochemiluminescence immunoassay (ECLIA). Continuous age-and sex-related percentiles (2.5th, 10th, 50th, 90th, 97.5th) were estimated, applying Cole’s LMS method. In both sexes, folate concentrations decreased continuously with age, whereas cobalamin concentration peaked between three and seven years of age and declined thereafter. Female sex was associated with higher concentrations of both vitamins in 13- to 18-year-olds and with higher folate levels in one- to five-year-olds. BMI was inversely correlated with concentrations of both vitamins, whilst SES positively affected folate but not cobalamin concentrations. To conclude, in the assessment of cobalamin and folate status, the age- and sex-dependent dynamic of the respective serum concentrations must be considered. While BMI is a determinant of both vitamin concentrations, SES is only associated with folate concentrations.

scholarly journals Cytotoxic and antigenotoxic properties of phenolic compound isolated from the fruit of Terminalia chebula on HeLa cell

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
K. Soumya ◽  
Jesna James ◽  
T. M. Archana ◽  
A. T. Dhanya ◽  
A. P. Shahid ◽  
...  
Keyword(s):  
Dna Damage ◽  
Gallic Acid ◽  
Phenolic Compound ◽  
Diethyl Ether ◽  
Hela Cells ◽  
Protective Role ◽  
Terminalia Chebula ◽  
Diethyl Ether Extract ◽  
Toxic Concentration ◽  
Isolated Compound

Abstract Background DNA in a human cell is subjected to constant assault from both environmental factors and normal metabolic processes. Accumulation of DNA damage drives the progression of many health disorders like aging, cancer, diabetes, and neurodegenerative disorders. Results The present study focuses on the isolation of phenolic compound from the fruit of Terminalia chebula and its protective role on induced DNA damage. Diethyl ether and ethyl acetate extract of Terminalia chebula fruit were subjected to column chromatographic purification, and the fractions obtained were tested for the presence of phenolics. Fraction-12 isolated from diethyl ether extract was identified as gallic acid, which is used for cytotoxic and DNA damage protection activity assays. To select a non-toxic concentration of isolated compound, cytotoxicity was assessed by MTT assay. Gallic acid showed moderate toxicity at the highest concentration tested (i.e., percentage cell viability at 100 μg/ml is 40.51 ± 1.31). Antigenotoxic effect of gallic acid on HeLa cells was carried by alkaline comet assay. The compound showed significant protective abilities against hydrogen peroxide-induced DNA damage in HeLa cells. Conclusion These results show the importance of gallic acid isolated from Terminalia chebula fruit, as protector of oxidative stress-induced DNA damage.

Prospective enzymes for omega-3 PUFA biosynthesis found in endoparasitic classes within the phylum Platyhelminthes

2020 ◽  
Vol 94 ◽  
Author(s):  
D. Babaran ◽  
M.T. Arts ◽  
R.J. Botelho ◽  
S.A. Locke ◽  
J. Koprivnikar
Keyword(s):  
Parasite Species ◽  
De Novo ◽  
Genomic Data ◽  
Wide Distribution ◽  
Omega 3 ◽  
Free Living ◽  
Chain Pufa ◽  
Pufa Biosynthesis ◽  
Aquatic Food ◽  
Long Chain

Abstract The free-living infectious stages of macroparasites, specifically, the cercariae of trematodes (flatworms), are likely to be significant (albeit underappreciated) vectors of nutritionally important polyunsaturated fatty acids (PUFA) to consumers within aquatic food webs, and other macroparasites could serve similar roles. In the context of de novo omega-3 (n-3) PUFA biosynthesis, it was thought that most animals lack the fatty acid (FA) desaturase enzymes that convert stearic acid (18:0) into ɑ-linolenic acid (ALA; 18:3n-3), the main FA precursor for n-3 long-chain PUFA. Recently, novel sequences of these enzymes were recovered from 80 species from six invertebrate phyla, with experimental confirmation of gene function in five phyla. Given this wide distribution, and the unusual attributes of flatworm genomes, we conducted an additional search for genes for de novo n-3 PUFA in the phylum Platyhelminthes. Searches with experimentally confirmed sequences from Rotifera recovered nine relevant FA desaturase sequences from eight species in four genera in the two exclusively endoparasite classes (Trematoda and Cestoda). These results could indicate adaptations of these particular parasite species, or may reflect the uneven taxonomic coverage of sequence databases. Although additional genomic data and, particularly, experimental study of gene functionality are important future validation steps, our results indicate endoparasitic platyhelminths may have enzymes for de novo n-3 PUFA biosynthesis, thereby contributing to global PUFA production, but also representing a potential target for clinical antihelmintic applications.

scholarly journals Fear conditioning and stimulus generalization in association with age in children and adolescents

2021 ◽  
Author(s):  
Julia Reinhard ◽  
Anna Slyschak ◽  
Miriam A. Schiele ◽  
Marta Andreatta ◽  
Katharina Kneer ◽  
...  
Keyword(s):  
Children And Adolescents ◽  
Fear Conditioning ◽  
Repeated Measures ◽  
Conditioned Fear ◽  
Fear Learning ◽  
Stimulus Generalization ◽  
Healthy Children ◽  
Older Individuals ◽  
Age Related ◽  
Current Task

AbstractThe aim of the study was to investigate age-related differences in fear learning and generalization in healthy children and adolescents (n = 133), aged 8–17 years, using an aversive discriminative fear conditioning and generalization paradigm adapted from Lau et al. (2008). In the current task, participants underwent 24 trials of discriminative conditioning of two female faces with neutral facial expressions, with (CS+) or without (CS−) a 95-dB loud female scream, presented simultaneously with a fearful facial expression (US). The discriminative conditioning was followed by 72 generalization trials (12 CS+, 12 GS1, 12 GS2, 12 GS3, 12 GS4, and 12 CS−): four generalization stimuli depicting gradual morphs from CS+ to CS− in 20%-steps were created for the generalization phases. We hypothesized that generalization in children and adolescents is negatively correlated with age. The subjective ratings of valence, arousal, and US expectancy (the probability of an aversive noise following each stimulus), as well as skin conductance responses (SCRs) were measured. Repeated-measures ANOVAs on ratings and SCR amplitudes were calculated with the within-subject factors stimulus type (CS+, CS−, GS1-4) and phase (Pre-Acquisition, Acquisition 1, Acquisition 2, Generalization 1, Generalization 2). To analyze the modulatory role of age, we additionally calculated ANCOVAs considering age as covariate. Results indicated that (1) subjective and physiological responses were generally lower with increasing age irrespective to the stimulus quality, and (2) stimulus discrimination improved with increasing age paralleled by reduced overgeneralization in older individuals. Longitudinal follow-up studies are required to analyze fear generalization with regard to brain maturational aspects and clarify whether overgeneralization of conditioned fear promotes the development of anxiety disorders or vice versa.

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