Antinociceptive and Anti-Inflammatory Activities of the Sesame Oil and Sesamin

Background Dietary intervention to prevent atherosclerosis and inflammation has been a major focus in recent years. Sesame oil (SO), widely used in many Asian countries, has been reported to help reduce high blood pressure. It has also been shown to reduce plasma cholesterol, low density lipoprotein (LDL) cholesterol and triglyceride levels. We previously reported that SO was effective in inhibiting atherosclerosis in LDL-receptor negative mice. In this study we tested whether the aqueous, non-lipid components of SO might have anti-inflammatory effects. Methods Sesame oil was extracted using ethanol:water mixture, lyophilized and reconstituted in water. To study anti-inflammatory effect, RAW 264.7 cells (macrophage cell line) were treated with the aqueous extract in the presence or absence of lipopolysaccharide (LPS) for 24 hours. RNA was extracted using Trizol. mRNA expression of inflammatory cytokines such as IL-1α, IL-6 and TNF-α were analyzed by real time PCR. Protein expression was determined by western blot analysis. To identify the mechanism of action, we performed luciferase assay using HepG2-LXR reporter cell lines. Results LPS induced the expression of IL-1α, IL-6 and TNF-α mRNA levels in RAW cells. The extract alone did not significantly affect the expressions of inflammatory cytokine genes. However, when treated together with LPS, sesame oil aqueous extract inhibited the mRNA levels of these cytokines significantly. Treatment with LPS together with SO extract also decreased the protein expression of these cytokines. The SO extract induced LXR expression as identified by the luciferase assay system in HepG2-LXR reporter cells. Conclusion These findings suggest that the aqueous portion of SO might be effective in preventing inflammation. Furthermore, the activation of LXR might suggest additional effects on lipid metabolism. Identifying the specific components present in the aqueous extract will be instrumental in developing treatment modalities for atherosclerosis and other inflammatory conditions.

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Non-Invasive Delivery of Nano-Emulsified Sesame Oil-Extract of Turmeric Attenuates Lung Inflammation

Pharmaceutics ◽

10.3390/pharmaceutics12121206 ◽

2020 ◽

Vol 12 (12) ◽

pp. 1206

Author(s):

Sahibzada Tasleem Rasool ◽

Rajasekhar Reddy Alavala ◽

Umasankar Kulandaivelu ◽

Nagaraja Sreeharsha

Keyword(s):

Lung Injury ◽

Bronchoalveolar Lavage ◽

Lung Inflammation ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Treated Group ◽

Sesame Oil ◽

Oil Extract ◽

Anti Inflammatory ◽

Nano Emulsion

Turmeric, the golden Indian spice, and the edible oil of sesame seeds are the essential ingredients of Indian food created by ancestors and established the belief of the curative effect of food for many generations. Considering the anti-inflammatory effects of turmeric, we formulated a nano-emulsion of turmeric infused in edible sesame oil, with a globule size of 200–250 nm using high-energy microfluidization. The product with a zeta potential of −11.5 mV showed spherical globules when imaged for scanning and transmission electron microscopy. We explored the anti-inflammatory potential of this edible nano-emulsion in lung inflammation. The lungs are the internal organ most vulnerable to infection, injury, and rapid inflammation from the external environment because of their constant exposure to pollutants, pathogenic microorganisms, and viruses. We evaluated the nano-emulsion for efficacy in ovalbumin-induced lung injury in mice with an oral treatment for two weeks. The therapeutic effect of nano-emulsion of the sesame oil-extract of turmeric was evident from biochemical analysis of bronchoalveolar lavage fluid, lung histopathology, and flow cytometric analysis. The developed nano-emulsion significantly reduced the inflammation and damage to the alveolar network in ovalbumin-injured mice. Significant reduction in the levels of neutrophils and inflammatory cytokines like IL-4, IL-6, and IL-13 in bronchoalveolar lavage fluid was observed in the nano-emulsion-treated group. Leukotriene B4 and IgE were also significantly altered in the treated group, thus suggesting the suitability of the formulation for the treatment of allergy and other inflammatory conditions. The nano-emulsification process potentiated the immunoregulatory effect of turmeric, as observed from the elevated levels of the natural anti-inflammatory cytokine, IL-10. The dietary constituents-based nano-emulsion of spice turmeric helped in scavenging the free radicals in the injured lungs, thus modulating the inflammation pathway. This easily scalable formulation technology approach can therefore serve as a potential noninvasive and safe treatment modality for reducing lung inflammation in lung injury cases.

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Abstract 347: Water-soluble Components of Sesame Oil Reduces Inflammation and Atherosclerosis

Arteriosclerosis Thrombosis and Vascular Biology ◽

10.1161/atvb.35.suppl_1.347 ◽

2015 ◽

Vol 35 (suppl_1) ◽

Author(s):

Chandrakala Aluganti Narasimhulu ◽

Krithika Selvarajan ◽

Kathryn Burge ◽

Dmitry Litvinov ◽

Bhaswati Sengupta ◽

...

Keyword(s):

High Fat Diet ◽

Plasma Lipids ◽

Cholesterol Metabolism ◽

Plasma Cholesterol ◽

Sesame Oil ◽

Water Soluble ◽

Gene Analysis ◽

High Fat ◽

Atherosclerotic Lesions ◽

Anti Inflammatory

Background: Atherosclerosis, a major form of cardiovascular disease, has now been recognized as a chronic inflammatory disease. Non-pharmacological means of treating chronic diseases have gained attention recently. We previously reported that the sesame oil aqueous extract (SOAE) has anti-inflammatory properties both in vitro and in vivo. In this study, we have determined whether SOAE has anti-atherosclerotic properties, and mechanisms by which it might modulate atherosclerosis by identifying genes and inflammatory markers. Methods and results: Low-density lipoprotein receptor knockout (LDLR-/-) female mice were fed with either high fat diet or high fat diet supplemented with SOAE. Plasma lipids and atherosclerotic lesions were quantified after 3 months of feeding. Plasma samples were used for global cytokine array. RNA was extracted from the liver tissue and the aorta and used for gene analysis. The SOAE-supplemented high fat diet significantly reduced atherosclerotic lesions, plasma cholesterol, and LDL cholesterol levels in LDLR-/- mice. Plasma inflammatory cytokines were reduced, but not significantly, demonstrating an anti-inflammatory property of SOAE. Gene analysis showed that SOAE-supplemented high fat diet reduced the genes involved in inflammation, and induced genes involved in cholesterol metabolism and reverse cholesterol transport. Conclusion: In conclusion, our studies suggest that a SOAE-enriched diet could be an effective non-pharmacological treatment for atherosclerosis by controlling inflammation and regulating lipid metabolism.

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Analgesic, Anti-pyretic and Anti-inflammatory Activity of Dietary Sesame Oil in Experimental Animal Models

Pharmacologia ◽

10.5567/pharmacologia.2011.172.177 ◽

2011 ◽

Vol 2 (6) ◽

pp. 172-177 ◽

Cited By ~ 11

Author(s):

T.S. Mohamed Saleem ◽

S. Darbar Basha ◽

G. Mahesh ◽

P.V. Sandhya Rani ◽

N. Suresh Kumar ◽

...

Keyword(s):

Animal Models ◽

Experimental Animal ◽

Sesame Oil ◽

Inflammatory Activity ◽

Experimental Animal Models ◽

Anti Inflammatory

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Anti-Atherosclerotic and Anti-Inflammatory Actions of Sesame Oil

Journal of Medicinal Food ◽

10.1089/jmf.2014.0138 ◽

2015 ◽

Vol 18 (1) ◽

pp. 11-20 ◽

Cited By ~ 31

Author(s):

Chandrakala Aluganti Narasimhulu ◽

Krithika Selvarajan ◽

Dmitry Litvinov ◽

Sampath Parthasarathy

Keyword(s):

Sesame Oil ◽

Anti Inflammatory

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Anti-Inflammatory, Antioxidative, and Hepatoprotective Effects of TransΔ9-Tetrahydrocannabinol/Sesame Oil on Adjuvant-Induced Arthritis in Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/9365464 ◽

2018 ◽

Vol 2018 ◽

pp. 1-13 ◽

Cited By ~ 4

Author(s):

Morouj Ismail ◽

Hiba Hasan ◽

Youmna El-Orfali ◽

Hanan Ismail ◽

Ghada Khawaja

Keyword(s):

Side Effects ◽

Interleukin 1 ◽

Clinical Signs ◽

Clinical Manifestations ◽

Antioxidant Defense System ◽

Sesame Oil ◽

Necrosis Factor Alpha ◽

Adjuvant Induced Arthritis ◽

First Line Therapy ◽

Anti Inflammatory

Rheumatoid arthritis (RA) is a painful chronic autoimmune disease affecting the joints. Its first-line therapy, Methotrexate (MTX), although effective in ameliorating the progress of the disease, induces hepatotoxicity over long-term usage. Thus, seeking natural compounds with fewer side effects could be an alternative therapeutic approach. This study aimed to investigate the anti-inflammatory, antiarthritic, and antioxidative effects of synthetictrans-Δ9-tetrahydrocannabinol (Δ9-THC) dissolved in sesame oil (Dronabinol) against MTX in adjuvant-induced arthritis (AIA) rat model. Daily oral administration of Δ9-THC/sesame oil, over a period of 21 days, was well tolerated in arthritic rats with no particular psychoactive side effects. It markedly attenuated the severity of clinical manifestations, recovered the histopathological changes in tibiotarsal joints, and repressed the splenomegaly in arthritic rats. Δ9-THC/sesame oil therapy showed similar effects to MTX in neutralizing the inflammatory process of AIA, through attenuating erythrocyte sedimentation rate (ESR) scores and proinflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin 1-beta (IL-1β), and interleukin-6 (IL-6) levels, to normal values. As opposed to MTX, this natural combination markedly protected the liver of arthritic rats and downregulated the induced oxidative stress by increasing the antioxidant defense system such as activities of catalase and superoxide dismutase (SOD) and levels of glutathione (GSH). These results suggest promising effects for the clinical use of Δ9-THC/sesame oil therapy in alleviating arthritic clinical signs as well as arthritis-induced liver injury.

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Anti-Inflammatory and Antioxidant Activities of the Nonlipid (Aqueous) Components of Sesame Oil: Potential Use in Atherosclerosis

Journal of Medicinal Food ◽

10.1089/jmf.2014.0139 ◽

2015 ◽

Vol 18 (4) ◽

pp. 393-402 ◽

Cited By ~ 17

Author(s):

Krithika Selvarajan ◽

Chandrakala Aluganti Narasimhulu ◽

Reena Bapputty ◽

Sampath Parthasarathy

Keyword(s):

Antioxidant Activities ◽

Sesame Oil ◽

Anti Inflammatory ◽

Potential Use

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Anti-inflammatory and Antioxidant Effects of Sesame Oil on Atherosclerosis: A Descriptive Literature Review

Cureus ◽

10.7759/cureus.1438 ◽

2017 ◽

Cited By ~ 6

Author(s):

Edmund Hsu ◽

Sam Parthasarathy

Keyword(s):

Literature Review ◽

Sesame Oil ◽

Anti Inflammatory ◽

Antioxidant Effects

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Healing Effect of Sesame Ointment on Second-degree Burn Wound in Rats

Galen Medical Journal ◽

10.31661/gmj.v5i2.658 ◽

2016 ◽

Vol 5 (2) ◽

pp. 56-62

Author(s):

Setareh Tehrani ◽

Parisa Lotfi ◽

Sepideh Tehrani ◽

Ehsan Jangholi ◽

Hoda Aryan ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Treated Group ◽

Sesame Oil ◽

Control Group ◽

Burn Wound ◽

Matrix Deposition ◽

Degree Burn ◽

Anti Inflammatory ◽

Second Degree

Background: Wound healing requires processes like cell migration, proliferation of extracellular matrix deposition, remodeling and angiogenesis. Also, anti-oxidative and anti-inflammatory reactions play key roles in the progression of wound healing. Previous studies showed that sesame oil has anti-inflammatory, antimicrobial and antioxidant activities. In addition, it is shown that sesame oil stimulates fibroblast production in vitro and in skin wounds. Therefore, in this study we investigated the effects of sesame ointment on the process of second-degree burn wound healing in rat models according to stereological parameters. Materials and Methods: In this experimental study, forty male Wistar rats (200±20 g) were randomly divided into four groups (n=10): ointment-base treated group (vehicle), silver sulfadiazine (SDD) treated group, sesame treated group (E1) and the control group which received no treatment. A 2×3 cm2 standard second-degree burn wound was induced on the posterior surface of animal’s neck under general anesthesia. After sacrifice, the animal’s skin sample was fixed in buffered formaldehyde for stereological evaluations. The data was analyzed by SPSS statistical software (version 14.0). P<0.05 was considered statistically significant. Results: According to our results, the mean of reduction in wound areas, volume density of collagen bundles and hair folicules, fibroblast populations, length density of vessels in E1 group was significantly higher than control group (P<0.05). The differences between E1 and SSD were not statistically noticeable regarding the stereological parameters. Conclusion: According to stereological analysis, administration of sesame ointment showed the ability to improve wound healing process and tissue regeneration in the treatment of skin damages.[GMJ.2016;5(2):56-62]

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Basal Lamina Formation in DMBA Induced Mammary Carcinoma

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080109 ◽

1977 ◽

Vol 35 ◽

pp. 534-535

Author(s):

I. Russo ◽

J. Saby ◽

J. Russo

Keyword(s):

Mammary Carcinoma ◽

Virgin Female ◽

Mammary Glands ◽

Sesame Oil ◽

Female Rats ◽

Ultrastructural Changes ◽

Post Inoculation ◽

Intermediate Type ◽

Sprague Dawley ◽

Intercellular Spaces

It has been previously demonstrated that DMBA-induced rat mammary carcinoma originates in the terminal end bud (TEB) of the mammary gland by proliferation of intermediate type cells (1). The earliest lesion identified is the intraductal proliferation (IDP), which gives rise to intraductal carcinomas. These evolve to cribriform, papillary and comedo types (2). In the present work, we report the ultrastructural changes that take place in the IDP for the formation of a cribriform pattern.Fifty-five-day-old Sprague Dawley virgin female rats were inoculated intra- gastrically with 20 mg 7,12-dimethylbenz(a)anthracene (DMBA) in 1 ml sesame oil. Non-inoculated, age-matched females were used as controls. Mammary glands from both control and experimental rats were removed weekly from the time of inoculation until 86 days post-inoculation. The glands were fixed and processed for electron microscopy (2).The first change observed in IDP's was the widening of intercellular spaces and the secretion of an electron dense material into these spaces (Fig. 1).

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