scholarly journals Mesoporous Calcium-Silicate Nanoparticles Loaded with Low-Dose Triton-100+Ag+ to Achieve Both Enhanced Antibacterial Properties and Low Cytotoxicity for Dentin Disinfection of Human Teeth

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1518
Author(s):  
Mengting Duan ◽  
Wei Fan ◽  
Bing Fan
Keyword(s):  
Sustained Release ◽  
Calcium Silicate ◽  
Low Dose ◽  
Ph Value ◽  
Membrane Integrity ◽  
Antibacterial Properties ◽  
Filling Material ◽  
Human Teeth ◽  
Dentinal Tubules ◽  
Low Cytotoxicity

Mesoporous calcium-silicate nanoparticles (MCSNs) are excellent biomaterials for controlled drug delivery and mineralization induction. In this study, MCSNs were loaded with low-dose silver ion (Ag+) and Triton X-100 (TX-100) as the M-AgTX to achieve both enhanced antibacterial properties and low cytotoxicity for dentin disinfection. The physicochemical property, biocompatibility, infiltration ability into dentinal tubules, anti-bacterial ability against both planktonic Enterococcusfaecalis (E. faecalis) and its biofilm on dentin, effects on dentin microhardness and in vitro mineralization property were systematically investigated. Results confirmed that the MCSNs and M-AgTX nanoparticles showed typical morphology of mesoporous materials and exhibited sustained release of chemicals with an alkaline pH value over time. M-AgTX also exhibited excellent biocompatibility on MC3T3-E1 cells and could eliminate 100% planktonic E. faecalis after 48-h treatment. On dentin slices, it could enter dentinal tubules by ultrasonic activation and inhibit the growth of E. faecalis on dentin. M-AgTX could completely inactive 28-day E. faecalis biofilm. TEM confirmed the destruction of cell membrane integrity and Ag+ infiltration into bacteria by M-AgTX. Besides, dentin slices medicated with M-AgTX nanoparticles displayed an increased microhardness. After being immersed in SBF for 7 days, apatite crystals could be observed on the surface of the material tablets. M-AgTX could be developed into a new multifunctional intra-canal medication or bone defect filling material for infected bone defects due to its sustained release profile, low cytotoxicity, infiltration ability, enhanced anti-bacterial and mineralization features.

scholarly journals Bioactive Properties of a Novel Antibacterial Dye Obtained from Laccase-Mediated Oxidation of 8-Anilino-1-naphthalenesulfonic Acid

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 487
Author(s):  
Jolanta Polak ◽  
Marcin Grąz ◽  
Kamila Wlizło ◽  
Katarzyna Szałapata ◽  
Justyna Kapral-Piotrowska ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
High Efficiency ◽  
Ph Value ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Transformation Process ◽  
Textile Dye ◽  
Bioactive Properties ◽  
Low Cytotoxicity ◽  
Mediated Oxidation

Fungal laccase obtained from a Cerrena unicolor strain was used as an effective biocatalyst for the transformation of 8-anilino-1-naphthalenesulfonic acid into a green-coloured antibacterial compound, which can be considered as both an antimicrobial agent and a textile dye, simultaneously. The process of biosynthesis was performed in buffered solutions containing methanol as a co-solvent, allowing better solubilisation of substrate. The transformation process was optimised in terms of the buffer pH value, laccase activity, and concentrations of the substrate and co-solvent. The crude product obtained exhibited low cytotoxicity, antibacterial properties against Staphylococcus aureus and Staphylococcus epidermidis, and antioxidant properties. Moreover, the synthesised green-coloured compound proved non-allergenic and demonstrated a high efficiency of dyeing wool fibres.

scholarly journals Mesoporous calcium silicate nanoparticles for superficial dental tissue reconstruction, in vitro and in vivo

RSC Advances ◽  
2021 ◽  
Vol 11 (40) ◽  
pp. 24681-24693
Author(s):  
Yixue Gao ◽  
Pin Huang ◽  
Ruiying Chen ◽  
Man Wang ◽  
Yining Wang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Calcium Silicate ◽  
Antibacterial Properties ◽  
Dental Tissue ◽  
Dentinal Tubules ◽  
Tissue Reconstruction

MCSNs could be used as a promising biomaterial for occluding the dentinal tubules in vitro and in vivo. Also, the outstanding drug delivery and antibacterial properties enable it to carry antibiotics easily for inhibiting deeper pulp infection.

scholarly journals Comparison of root canal walls cleanliness obturated with two commercially available; calcium silicate sealers and a resin sealer after retreatment

2020 ◽  
Vol 5 (1) ◽  
pp. 20-23
Author(s):  
Mai H. Abdelrahman ◽  
◽  
Mohamed Y Hassan ◽  
Keyword(s):  
Root Canal ◽  
Calcium Silicate ◽  
Complete Removal ◽  
Filling Material ◽  
Dentinal Tubule ◽  
Universal System ◽  
Dentinal Tubules ◽  
Root Canal Retreatment ◽  
Root Canal Sealers ◽  
Scanning Electron

Background: Despite improvements and advances in materials and methods, root canal treatment is not always satisfactory. After completing the obturation procedures, retreatment might be mandatory in cases of reinfection or inappropriately cleaned root canal. Retreatment strategy involves elimination of the obturating material, and then the whole mechanical and filling procedures are repeated again. Thus, root canal sealers removal shouldn’t represent an obstacle. Objective: The current study aimed is to investigate and observe the cleanliness of root canal walls that were previously obturated with two types of calcium silicate bioceramic sealers compared to a resin sealer. Materials and Methods: 21 single-rooted premolars were utilized. After complete removal of the obturating materials using Protaper retreatment universal system, the roots were cut by the use of a hammer and a chisel, then the middle third of the retreated canals were observed under a scanning electron microscope at magnification 1000x, the amount of clear, opened dentinal tubules was compared. Results: Among the three investigated sealers, ADSEAL showed the largest number of clear dentinal tubules while, a fewer open dentinal tubule were observed with Well Root and Ceraseal. Conclusions: None of the investigated sealers could be totally removed from root canal walls. However, the quantity of remnants of root filling material with well root and ceraseal were significantly more, compared to ADSEAL.

scholarly journals Implantation and Retrieval of Magnetic Nanobots for Treatment of Endodontic Re-Infection in Human Dentine

2021 ◽  
Author(s):  
Debayan Dasgupta ◽  
Shanmukh Srinivas Peddi ◽  
Deepak K. Saini ◽  
Ambarish Ghosh
Keyword(s):  
Root Canal ◽  
Treatment Method ◽  
Therapeutic Modality ◽  
Clinical Practices ◽  
Human Teeth ◽  
Dentinal Tubules ◽  
Current State ◽  
Human Dentine ◽  
New Treatment ◽  
Magnetic Drive

<div> <div> <div> <p>More than 10% of root canal treatments undergo failure worldwide due to remnant bacteria deep in the dentinal tubules located within the dentine tissue of human teeth. Owing to the complex and narrow geometry of the tubules, current techniques relying on passive diffusion of anti-bacterial agents are inadequate. Here, we present a new treatment method using actively maneuvered nanobots, which can be incorporated during standard root canal procedure. Our technique will enable dentists to execute procedures inside the dentine not yet possible by current state of the art. We demonstrate that magnetically driven nanobots can reach the depths of the tubules up to hundred times faster than current clinical practices. Subtle modifications of the magnetic drive allowed deep implantation of the nanobots isotopically distributed throughout the dentine, along with spatially controlled retrieval from selected areas. Finally, we demonstrate the integration of bactericidal therapeutic modality with the nanobots, thereby validating the tremendous potential of nanobots in dentistry, and nanomedicine in general. </p> </div> </div> </div>

scholarly journals Large-Scale Screening of 239 Traditional Chinese Medicinal Plant Extracts for Their Antibacterial Activities against Multidrug-Resistant Staphylococcus aureus and Cytotoxic Activities

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 185 ◽  
Author(s):  
Gowoon Kim ◽  
Ren-You Gan ◽  
Dan Zhang ◽  
Arakkaveettil Kabeer Farha ◽  
Olivier Habimana ◽  
...  
Keyword(s):  
Large Scale ◽  
Salvia Miltiorrhiza ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Multidrug Resistant ◽  
Cytotoxic Activities ◽  
Phenolic Contents ◽  
Medicinal Plant Extracts ◽  
Low Cytotoxicity

Novel alternative antibacterial compounds have been persistently explored from plants as natural sources to overcome antibiotic resistance leading to serious foodborne bacterial illnesses. In this study, the ethanolic extracts from 239 traditional Chinese medicinal plants (TCMP)’ materials were screened to discover promising candidates that have strong antibacterial properties against multidrug-resistant Staphylococcus (S.) aureus and low cytotoxicity. The results revealed that 74 extracts exhibited good antibacterial activities (diameter of inhibition zone (DIZ) ≥ 15 mm). Furthermore, 18 extracts (DIZ ≥ 20 mm) were determined their minimum inhibitory concentrations (MIC) and minimum bactericide concentrations (MBC), ranging from 0.1 to 12.5 mg/mL and 0.78 to 25 mg/mL, respectively. In addition, most of the 18 extracts showed relatively low cytotoxicity (a median lethal concentration (LC50) >100 µg/mL). The 18 extracts were further determined to estimate possible correlation of their phenolic contents with antibacterial activity, and the results did not show any significant correlation. In conclusion, this study selected out some promising antibacterial TCMP extracts with low cytotoxicity, including Rhus chinensis Mill., Ilex rotunda Thunb., Leontice kiangnanensis P.L.Chiu, Oroxylum indicum Vent., Isatis tinctorial L., Terminalia chebula Retz., Acacia catechu (L.f.) Willd., Spatholobus suberectus Dunn, Rabdosia rubescens (Hemsl.) H.Hara, Salvia miltiorrhiza Bunge, Fraxinus fallax Lingelsh, Coptis chinensis Franch., Agrimonia Pilosa Ledeb., and Phellodendron chinense C.K.Schneid.

scholarly journals Effects of Dentifrices Containing Nanohydroxyapatite on Dentinal Tubule Occlusion—A Scanning Electron Microscopy and EDX Study

2020 ◽  
Vol 10 (18) ◽  
pp. 6513
Author(s):  
Emilia Bologa ◽  
Simona Stoleriu ◽  
Gianina Iovan ◽  
Cristina Angela Ghiorghe ◽  
Irina Nica ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Study Groups ◽  
Control Group ◽  
Dentinal Tubule ◽  
Human Teeth ◽  
Dentinal Tubules ◽  
Mineral Deposition ◽  
Tubule Occlusion ◽  
Scanning Electron

This in vitro study evaluated the effects of dentifrices containing nano-hydroxyapatite (n-HAp) on dentinal tubule occlusion and on mineral deposition. Dentin specimens of ten human teeth were submersed for 30 s in 40% citric acid and then randomly divided into four groups (three study groups and one control group). In the study groups, the dentin samples were exposed to three different n-HAp toothpastes: Karex (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany), Biorepair Plus Sensitive (Coswell SpA, Bologna, Italy), and Dr. Wolff’s Biorepair (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany); in the control group no toothpaste was applied. All of the samples were evaluated using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis. In the control group all of the samples showed a frank and wide opening of the dentinal tubules, whereas in the study groups different degrees of tubule closure by mineral depositions were observed. Toothpastes containing n-HAp determined a significant occlusion of dentinal tubules and a significant increase of mineral deposition on the dentin surface. All three tested toothpastes showed similar results regarding the degree of dentinal tubule closure. Varying degrees of differences in calcium, phosphate, carbon, and oxygen ion concentrations among the three tested toothpastes were obtained.

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