scholarly journals 3D-Printed Mucoadhesive Collagen Scaffolds as a Local Tetrahydrocurcumin Delivery System

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1697
Author(s):  
Mireia Andonegi ◽  
Teresa Carranza ◽  
Alaitz Etxabide ◽  
Koro de la Caba ◽  
Pedro Guerrero
Keyword(s):  
3D Printing ◽  
Fibrillar Structure ◽  
3D Printer ◽  
X Ray Diffraction ◽  
Collagen Scaffolds ◽  
Scanning Calorimetry ◽  
X Ray ◽  
Native Collagen ◽  
3D Printed ◽  
Physical Interactions

Native collagen doughs were processed using a syringe-based extrusion 3D printer to obtain collagen scaffolds. Before processing, the rheological properties of the doughs were analyzed to determine the optimal 3D printing conditions. Samples showed a high shear-thinning behavior, reported beneficial in the 3D printing process. In addition, tetrahydrocurcumin (THC) was incorporated into the dough formulation and its effect on collagen structure, as well as the resulting scaffold’s suitability for wound healing applications, were assessed. The denaturation peak observed by differential scanning calorimetry (DSC), along with the images of the scaffolds’ surfaces assessed using scanning electron microscopy (SEM), showed that the fibrillar structure of collagen was maintained. These outcomes were correlated with X-ray diffraction (XRD) results, which showed an increase of the lateral packaging of collagen chains was observed in the samples with a THC content up to 4%, while a higher content of THC considerably decreased the structural order of collagen. Furthermore, physical interactions between collagen and THC molecules were observed using Fourier transform infrared (FTIR) spectroscopy. Additionally, all samples showed swelling and a controlled release of THC. These results along with the mucoadhesive properties of collagen suggested the potential of these THC–collagen scaffolds as sustained THC delivery systems.

Preliminary Study of Alginates Extracted from Brown Algae (Sargassum sp.) Available in Madura Island as Composite Based Hydrogel Materials

2019 ◽  
Vol 964 ◽  
pp. 240-245 ◽  
Author(s):  
Amaliya Rasyida ◽  
Thalyta Rizkha Pradipta ◽  
Sigit Tri Wicaksono ◽  
Vania Mitha Pratiwi ◽  
Yeny Widya Rakhmawati
Keyword(s):  
Sodium Alginate ◽  
Brown Algae ◽  
Extraction Process ◽  
Composition Analysis ◽  
3D Printer ◽  
X Ray Diffraction ◽  
X Ray ◽  
3D Printed ◽  
Preliminary Study

Utilization of brown algae especially in Madura, where it’s close to Surabaya, only limited for food. This become a reference for developing and increasing the potential of this algae by extracting one of the ingredients, namely alginate. This paper deals with the characterization of sodium alginate extracted from sargassum sp. using modified-purified calcium routes. The extracted sodium alginate will be further used as composite hydrogel materials and compared with commercial sodium alginate. Hereafter, the synthesized composite is expected to be bio-ink for 3d printer. Chemical composition analysis were analyzed using X-Ray Fluorosense (XRF) followed by Fourier-transform infrared spectroscopy (FTIR) analysis to identify the functional group of composite and X-Ray Diffraction (XRD). Furthermore, viscosity bath is performed to compare the viscosity of extracted and commercial one. The result shows that modified-purified calcium routes in the extraction process of sodium alginate is desirable for improving their properties. Interestingly enough, with the goal of using it as bio-ink in 3d printed fabrication, the synthesized composite shows viscosity, 300 cSt, which meets the criteria for bio-ink in 3d printer.

Effects of Pea Protein on the Properties of Potato Starch-Based 3D Printing Materials

2018 ◽  
Vol 14 (3) ◽  
Author(s):  
Feng Chuanxing ◽  
Wang Qi ◽  
Li Hui ◽  
Zhou Quancheng ◽  
Meng Wang
Keyword(s):  
3D Printing ◽  
Chemical Structure ◽  
Potato Starch ◽  
Textural Properties ◽  
Theoretical Research ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
Pea Protein ◽  
X Ray ◽  
Printing Quality

AbstractSince materials are the substantial foundation for 3D printing, the lack of theoretical research on 3D printing food materials restricts the development of 3D printing in food field. Based on the principle of 3D starch gelatinization printing, this study revealed the effects of pea protein on the printability of potato starch-based 3D printing materials in granular structure, crystalline structure, chemical structure, textural properties, and thermal properties, through scanning electron microscope, X-ray diffraction, Fourier-transformed infrared, differential scanning calorimetry, and textural method, and developed new food materials for 3D printing. The study indicated the structural properties and physicochemical properties changed regularly with the increase in the content of pea protein, when the content of pea protein was 1%, the printing quality was best. The study provides a new theory for the application of 3D printing in food production and technical support for actual production.

scholarly journals Biocompatibility of Developing 3D-Printed Tubular Scaffold Coated with Nanofibers for Bone Applications

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Febe Carolina Vazquez-Vazquez ◽  
Osmar Alejandro Chanes-Cuevas ◽  
David Masuoka ◽  
Jesús Arenas Alatorre ◽  
Daniel Chavarria-Bolaños ◽  
...  
Keyword(s):  
3D Printing ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Ease Of Use ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
Tubular Scaffold ◽  
3D Printed ◽  
Cell Material Interactions ◽  
Nanofiber Coating

3D printing with controlled microarchitectures has gained traction in a wide variety of fields, including bone tissue engineering, because it represents an exciting alternative for the synthesis of new scaffolds due to its rapid manufacturing process, high precision, cost-effectiveness, and ease of use. Thus, this study is aimed at evaluating the biocompatibility response of a 3D-printed tubular scaffold coated by a layer of 7% PLA nanofibers. The morphology, structure, and chemical composition of the 3D-printed tubular scaffold were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and surface property analysis by profilometry. The biocompatibility response of the scaffold was assessed by cell adhesion, proliferation, and cell-material interactions of human fetal osteoblasts. Our results showed that 3D printing allowed obtaining similar and reproducible structures and the biocompatibility assays showed that nanofiber coating of the surface of the 3D tubular scaffold promoted an improvement on cell attachment, proliferation, and the morphology of osteoblast cells when compared with a noncoated scaffold. In conclusion, the surface of the 3D-printed tubular scaffold could be improved by the deposition of a nanofiber layer to render a more mimetic and active topography with excellent cellular biocompatibility for bone tissue applications.

scholarly journals A Green Approach towards Native Collagen Scaffolds: Environmental and Physicochemical Assessment

Polymers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1597
Author(s):  
Mireia Andonegi ◽  
Ainhoa Irastorza ◽  
Ander Izeta ◽  
Sara Cabezudo ◽  
Koro de la Caba ◽  
...  
Keyword(s):  
Global Strategy ◽  
X Ray Diffraction ◽  
Collagen Scaffolds ◽  
Green Approach ◽  
Use Of Resources ◽  
Native Collagen ◽  
Physical Interactions ◽  
Porous Microstructures ◽  
The Impact ◽  
Compression Resistance

Native collagen scaffolds were prepared in this work, in which both materials and environmental approaches were considered with the aim of providing a global strategy towards more sustainable biomaterials. From the environmental perspective, it is worth mentioning that acid and enzymatic treatments have been avoided to extract collagen, allowing the reduction in the use of resources, in terms of chemicals, energy, and time, and leading to a low environmental load of this step in all the impact categories under analysis. With the incorporation of chitosan into the scaffold-forming formulations, physical interactions occurred between collagen and chitosan, but the native collagen structure was preserved, as observed by Fourier transform infrared (FTIR) and X-ray diffraction (XRD) analyses. The incorporation of chitosan also led to more homogenous porous microstructures, with higher elastic moduli and compression resistance for both dry and hydrated scaffolds. Furthermore, hydrated scaffolds preserved their size and shape after some compression cycles.

Studies on the Preparation, Characterization and Solubility of -Cyclodextrin-Roxythromycin Inclusion Complexes

2009 ◽  
Vol 2 (2) ◽  
pp. 523-530
Author(s):  
D. Nagasamy Venkatesh ◽  
S. Karthick ◽  
M. Umesh ◽  
G. Vivek ◽  
R.M. Valliappan ◽  
...  
Keyword(s):  
Dissolution Rate ◽  
Inclusion Complexes ◽  
Phase Solubility ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
X Ray ◽  
Ir Studies ◽  
Poorly Soluble ◽  
Poorly Soluble Drug

Roxythromycin/ β-cyclodextrin (Roxy/ β-CD) dispersions were prepared with a view to study the influence of β-CD on the solubility and dissolution rate of this poorly soluble drug. Phase-solubility profile indicated that the solubility of roxythromycin was significantly increased in the presence of β-cyclodextrin and was classified as AL-type, indicating the 1:1 stoichiometric inclusion complexes. Physical characterization of the prepared systems was carried out by differential scanning calorimetry (DSC), X-ray diffraction studies (XRD) and IR studies. Solid state characterization of the drug β-CD binary system using XRD, FTIR and DSC revealed distinct loss of drug crystallinity in the formulation, ostensibly accounting for enhancement of dissolution rate.

scholarly journals A review of nanotechnology with an emphasis on Nanoplex

2015 ◽  
Vol 51 (2) ◽  
pp. 255-263
Author(s):  
Rupali Nanasaheb Kadam ◽  
Raosaheb Sopanrao Shendge ◽  
Vishal Vijay Pande
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Drug Loading ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
X Ray ◽  
Conventional Drug ◽  
Anionic Drugs ◽  
Oppositely Charged ◽  
The Brain

<p>The use of nanotechnology based on the development and fabrication of nanostructures is one approach that has been employed to overcome the challenges involved with conventional drug delivery systems. Formulating Nanoplex is the new trend in nanotechnology. A nanoplex is a complex formed by a drug nanoparticle with an oppositely charged polyelectrolyte. Both cationic and anionic drugs form complexes with oppositely charged polyelectrolytes. Compared with other nanostructures, the yield of Nanoplex is greater and the complexation efficiency is better. Nanoplex are also easier to prepare. Nanoplex formulation is characterized through the production yield, complexation efficiency, drug loading, particle size and zeta potential using scanning electron microscopy, differential scanning calorimetry, X-ray diffraction and dialysis studies. Nanoplex have wide-ranging applications in different fields such as cancer therapy, gene drug delivery, drug delivery to the brain and protein and peptide drug delivery.</p>

scholarly journals Multi-Scale Studies of 3D Printed Mn–Na–W/SiO2 Catalyst for Oxidative Coupling of Methane

Catalysts ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 290
Author(s):  
Tim Karsten ◽  
Vesna Middelkoop ◽  
Dorota Matras ◽  
Antonis Vamvakeros ◽  
Stephen Poulston ◽  
...  
Keyword(s):  
Oxidative Coupling ◽  
Oxidative Coupling Of Methane ◽  
Operating Conditions ◽  
Homogeneous Distribution ◽  
Silica Support ◽  
X Ray Diffraction ◽  
Cross Sectional ◽  
X Ray ◽  
Multi Scale ◽  
3D Printed

This work presents multi-scale approaches to investigate 3D printed structured Mn–Na–W/SiO2 catalysts used for the oxidative coupling of methane (OCM) reaction. The performance of the 3D printed catalysts has been compared to their conventional analogues, packed beds of pellets and powder. The physicochemical properties of the 3D printed catalysts were investigated using scanning electron microscopy, nitrogen adsorption and X-ray diffraction (XRD). Performance and durability tests of the 3D printed catalysts were conducted in the laboratory and in a miniplant under real reaction conditions. In addition, synchrotron-based X-ray diffraction computed tomography technique (XRD-CT) was employed to obtain cross sectional maps at three different positions selected within the 3D printed catalyst body during the OCM reaction. The maps revealed the evolution of catalyst active phases and silica support on spatial and temporal scales within the interiors of the 3D printed catalyst under operating conditions. These results were accompanied with SEM-EDS analysis that indicated a homogeneous distribution of the active catalyst particles across the silica support.

scholarly journals Diethylstilbestrol Modifies the Structure of Model Membranes and Is Localized Close to the First Carbons of the Fatty Acyl Chains

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 220
Author(s):  
Alessio Ausili ◽  
Inés Rodríguez-González ◽  
Alejandro Torrecillas ◽  
José A. Teruel ◽  
Juan C. Gómez-Fernández
Keyword(s):  
Membrane Surface ◽  
Water Layer ◽  
Fatty Acyl ◽  
31P Nmr Spectroscopy ◽  
Relaxation Rates ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
X Ray ◽  
Acyl Chains ◽  
Dynamics Simulations

The synthetic estrogen diethylstilbestrol (DES) is used to treat metastatic carcinomas and prostate cancer. We studied its interaction with membranes and its localization to understand its mechanism of action and side-effects. We used differential scanning calorimetry (DSC) showing that DES fluidized the membrane and has poor solubility in DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) in the fluid state. Using small-angle X-ray diffraction (SAXD), it was observed that DES increased the thickness of the water layer between phospholipid membranes, indicating effects on the membrane surface. DSC, X-ray diffraction, and 31P-NMR spectroscopy were used to study the effect of DES on the Lα-to-HII phase transition, and it was observed that negative curvature of the membrane is promoted by DES, and this effect may be significant to understand its action on membrane enzymes. Using the 1H-NOESY-NMR-MAS technique, cross-relaxation rates for different protons of DES with POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) protons were calculated, suggesting that the most likely location of DES in the membrane is with the main axis parallel to the surface and close to the first carbons of the fatty acyl chains of POPC. Molecular dynamics simulations were in close agreements with the experimental results regarding the location of DES in phospholipids bilayers.

scholarly journals Effects of Sintering Temperature on Densification, Microstructure and Mechanical Properties of Al-Based Alloy by High-Velocity Compaction

Metals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 218
Author(s):  
Xianjie Yuan ◽  
Xuanhui Qu ◽  
Haiqing Yin ◽  
Zaiqiang Feng ◽  
Mingqi Tang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
High Velocity ◽  
Sintered Density ◽  
Sintering Temperature ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
X Ray ◽  
Transmission Electron ◽  
High Velocity Compaction ◽  
Sintered Temperature

This present work investigates the effects of sintering temperature on densification, mechanical properties and microstructure of Al-based alloy pressed by high-velocity compaction. The green samples were heated under the flow of high pure (99.99 wt%) N2. The heating rate was 4 °C/min before 315 °C. For reducing the residual stress, the samples were isothermally held for one h. Then, the specimens were respectively heated at the rate of 10 °C/min to the temperature between 540 °C and 700 °C, held for one h, and then furnace-cooled to the room temperature. Results indicate that when the sintered temperature was 640 °C, both the sintered density and mechanical properties was optimum. Differential Scanning Calorimetry, X-ray diffraction of sintered samples, Scanning Electron Microscopy, Energy Dispersive Spectroscopy, and Transmission Electron Microscope were used to analyse the microstructure and phases.

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