scholarly journals Use of Conventional and Innovative Technologies for the Production of Food Grade Hop Extracts: Focus on Bioactive Compounds and Antioxidant Activity

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 41
Author(s):  
Veronica Santarelli ◽  
Lilia Neri ◽  
Katya Carbone ◽  
Valentina Macchioni ◽  
Paola Pittia
Keyword(s):  
Bioactive Compounds ◽  
Extraction Method ◽  
Industrial Process ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Total Carotenoids ◽  
Power Ultrasound ◽  
Food Grade ◽  
High Power Ultrasound ◽  
Almost All

This study investigated the use of conventional and innovative extraction methods to produce food-grade hop extracts with high antioxidant capacity and content in bioactive compounds. Conventional extractions (CONV) were performed under dynamic maceration at 25 and 60 °C; innovative extractions were performed using two ultrasound systems, a laboratory bath (US) and a high-power ultrasound bath (HPUS), and a high-pressure industrial process. For CONV, US, and HPUS extractions the effect of the extraction time was also tested. Experimental results showed that extraction method, temperature, and time affect to a different extent the phenolic profile and have a significant effect (p < 0.05) on the total phenolic content, total flavonoid content, antiradical capacity (ABTS), chlorophyll α, and total carotenoids content. Overall, US and CONV 60 °C extractions showed the highest extraction efficiency for almost all the investigated compounds, however, the extraction method and time to be used strongly depends on the target compounds to extract.

scholarly journals Seasonal Variation of Health-Promoting Bioactives in Broccoli and Methyl-Jasmonate Pre-Harvest Treatments to Enhance Their Contents

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1371 ◽  
Author(s):  
Vanesa Nuñez-Gómez ◽  
Nieves Baenas ◽  
Inma Navarro-González ◽  
Javier García-Alonso ◽  
Diego A. Moreno ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Methyl Jasmonate ◽  
Bioactive Compounds ◽  
High Performance ◽  
Total Phenolic ◽  
Diode Array ◽  
Total Carotenoids ◽  
Health Promoting

Broccoli is a source of bioactive compounds that provide an important nutritional value. The content of these compounds can vary depending on agronomic and environmental conditions, as well as on elicitation. In this study, three crop trials were carried out to evaluate the effects of the cultivation season, the application of different dosages of methyl-jasmonate (MeJA) on the overall quality and on the total content of bioactive compounds of ‘Parthenon’ broccoli cultivated under the field conditions of southeastern Spain. Color parameters, chlorophyll content, total phenolic compounds, total flavonoids and antioxidant activity were measured to evaluate the overall quality. Moreover, individual carotenoids, phenolic compounds and glucosinolates were evaluated by high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography equipped with diode array detector coupled to mass spectrometer using electro spray ionization (HPLC-DAD-ESI/MSn). The content of total carotenoids, phenolic compounds and glucosinolates were higher in autumn compared with spring, showing increases of 2.8-fold, 2-fold and 1.2-fold, respectively. Moreover, a double application of MeJA increased the contents of total carotenoids, phenolic compounds and glucosinolates by 22%, 32% and 39%, respectively, relative to the untreated samples. Considering our results, the controlled and timely application of 250 µM MeJA to the aerial parts of the plants four days before harvest, on two consecutive days, seems to be a valid agronomic strategy to improve the health-promoting capacity of Parthenon broccoli, without compromising its overall quality.

scholarly journals Obtaining Bioactive Compounds from the Coffee Husk (Coffea arabica L.) Using Different Extraction Methods

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 46
Author(s):  
Mariana de Oliveira Silva ◽  
John Nonvignon Bossis Honfoga ◽  
Lorena Lucena de Medeiros ◽  
Marta Suely Madruga ◽  
Taliana Kênia Alencar Bezerra
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Extraction Methods ◽  
Water Bath ◽  
Raw Material ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Ethanol Mixture ◽  
Coffee Husk ◽  
Coffea Arabica L

Coffee husks (Coffea arabica L.) are characterized by exhibiting secondary metabolites such as phenolic compounds, which can be used as raw material for obtaining bioactive compounds of interest in food. The objective of this study is to evaluate different methods for obtaining the raw material and extracting solutions of bioactive compounds from coffee husks. Water bath and ultrasound-assisted extraction methods were used, using water (100%) or ethanol (100%) or a mixture of both (1:1) as extracting solutions and the form of the raw material was in natura and dehydrated. The extracts were evaluated by their antioxidant potential using DPPH radicals, ABTS, and iron reduction (ferric reducing antioxidant power (FRAP)), and later total phenolic compounds, total flavonoids, and condensed tannins were quantified the phenolic majority compounds were identified. It was verified that the mixture of water and ethanol (1:1) showed better extraction capacity of the compounds with antioxidant activity and that both conventional (water bath) or unconventional (ultrasound) methods showed satisfactory results. Finally, a satisfactory amount of bioactive compounds was observed in evaluating the chemical composition (total phenolic compounds, total flavonoids, condensed tannins, as well as the analysis of the phenolic profile) of these extracts. Corroborating with the results of the antioxidant activities, the best extracting solution was generally the water and ethanol mixture (1:1) using a dehydrated husk and water bath as the best method, presenting higher levels of the bioactive compounds in question, with an emphasis on chlorogenic acid. Thus, it can be concluded that the use of coffee husk as raw material to obtain extracts of bioactive compounds is promising. Last, the conventional method (water bath) and the water and ethanol mixture (1:1) stood out among the methods and extracting solutions used for the dehydrated coffee husk.

scholarly journals Agro-industrial waste as a source of bioactive compounds: ultrasound-assisted extraction from blueberry (Vaccinium myrtillus) and raspberry (Rubus idaeus) pomace

2021 ◽  
Vol 43 ◽  
pp. e55564
Author(s):  
Suelen Siqueira dos Santos ◽  
Carolina Moser Paraíso ◽  
Letícia Misturini Rodrigues ◽  
Grasiele Scaramal Madrona
Keyword(s):  
Bioactive Compounds ◽  
Energy Efficient ◽  
Extraction Method ◽  
Rubus Idaeus ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Conventional Extraction ◽  
Gallic Acid Equivalent

Blueberry and raspberry pomace are a rich source of bioactive compounds that have not been commercially utilized yet, and ultrasound-assisted technology can efficiently extract these compounds. Also, the use of water as a solvent added to the ultrasound-assisted technology improves this eco-friendly process. Therefore, an aqueous eco-friendly extraction, including extraction time and ultrasound presence or absence (conventional extraction) was performed in order to extract bioactive compounds from blueberry and raspberry pomace. Response parameters included levels of anthocyanins, phenolic compounds, and flavonoids, and antioxidant activity determined by DPPH, ABTS, and FRAP methods. Analysis of variance results indicated that ultrasound-assisted extraction for 45 min. was feasible to extract the bioactive compounds. The antioxidant content of the extract obtained by the ultrasound-assisted process was 1.4 times higher on average and the total phenolic concentration was 1.6 times higher (for blueberry 5.02 and for raspberry 2.53 mg gallic acid equivalent/g) compared with those obtained by the conventional process. Thus, the ultrasound-assisted extraction method can be a profitable alternative to extract bioactive compounds from blueberry and raspberry pomace, as it is energy efficient, requires fewer chemicals, and produces less effluent. This eco-friendly technology is therefore viable for food, nutraceutical, and cosmetic industries, and also for reducing food waste.

The Effects of Solvents and Extraction Methods on the Antioxidant Activity of P. niruri

2014 ◽  
Vol 68 (5) ◽  
Author(s):  
Hafizah Mohd Hadzri ◽  
Mohd Azizi Che Yunus ◽  
Salman Zhari ◽  
Fahim Rithwan
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Soxhlet Extraction ◽  
Solvent Polarity ◽  
Extraction Methods ◽  
The Other ◽  
Natural Antioxidant ◽  
Total Phenolic

The effects of different types of solvents and extraction method were investigated to determine the presence of antioxidant contents and activity from the P. niruri plant. The aim of this study is to determine which extraction method will give higher natural antioxidant contents and antioxidant activity. The content of natural antioxidant and antioxidant activity were analysed by total phenolic content (TPC), total flavonoid content (TFC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The results showed that extracts from a supercritical fluid extraction (SFE) method without the addition of modifier showed the highest content of total phenolic (187.66 mg GAE/ g) and flavonoid (1100.93 mg QE/g) in P. niruri compared to the other methods of extraction with different type of solvents. The extract of P. niruri from different extraction methods showed antioxidant activity on DPPH radical scavenging assay. The soxhlet extraction method by methanol showed the lowest IC50 compared to the other methods of extraction. The results revealed that P. niruri extracts had different content of antioxidant and antioxidant activity. The solvent polarity and different methods of extraction play significant roles in determining the most suitable method for production of antioxidant contents and antioxidant activity from P. niruri extracts.

scholarly journals Ripe Ora-pro-nobis (Pereskia aculeata miller) fruits express high contents of bioactive compounds and antioxidant capacity

2018 ◽  
Vol 40 (3) ◽  
Author(s):  
Aline Priscilla Gomes da Silva ◽  
Poliana Cristina Spricigo ◽  
Thais Pádua de Freitas ◽  
Thiago Machado da Silva Acioly ◽  
Severino Matias de Alencar ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Maturity Stage ◽  
Total Phenolic Compounds ◽  
Total Carotenoids ◽  
Significant Difference

Abstract Pereskia aculeata Miller is a native cactaceae found from the Northeast to the South of Brazil. This plant is recognized by the high nutritional value of their leaves, which are the most used in cooking and folk medicine. However, studies on the chemical characteristics and antioxidant capacity of the ora-pro-nobis fruits are scarce. Therefore, the aim of this study was to determine the best harvest point of the ora-pro-nobis fruits to optimize the content of bioactive compounds and their antioxidant capacity. The fruits were manually harvested, defined by their peel colors in three maturity stages: unripe (green), intermediate (yellowish green), and ripe (yellow) stages. The following attributes were evaluated: soluble solids (SS), titratable acidity (TA), SS/TA ratio, pH, and content of bioactive compounds, such as total chlorophylls, carotenoids, yellow flavonoids, total phenolic compounds, and antioxidant capacity. There was a decrease in the content of chlorophyll followed by an increase in the of total carotenoids and yellow flavonoids, due to the maturity stage. For the total phenolic compounds, it was not observed significant difference between the stages. The antioxidant capacity was quantified by the ORAC method in lyophilized pulp in the unripe (1.95 mmol 100 g-1 trolox) and ripe (3.85 mmol 100 g-1 trolox) stages. The lowest contents were observed in the intermediate (0.80 mmol 100 g-1 trolox in lyophilized pulp) stage. The maturity stage significantly influenced the bioactive compounds of ora-pro-nobis, which presented higher values of total carotenoids, yellow flavonoids, and antioxidant capacity in ripe fruits.

scholarly journals Extraction Processes with Several Solvents on Total Bioactive Compounds in Different Organs of Three Medicinal Plants

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4672
Author(s):  
Nour El Houda Lezoul ◽  
Mohamed Belkadi ◽  
Fariborz Habibi ◽  
Fabián Guillén
Keyword(s):  
Bioactive Compounds ◽  
Extraction Method ◽  
Extraction Methods ◽  
Polyphenol Content ◽  
Medicinal Species ◽  
Total Polyphenol ◽  
Physalis Peruviana ◽  
Total Antioxidant ◽  
Extraction Processes ◽  
Different Parts

The extraction of secondary metabolites by water, MeOH:water (8:2) containing NaF, methanol, ethanol and acetone (all of them diluted (7:3) in water)from the different parts (leaves, flowers, stems and roots) of Passiflora caerulea L., Physalis peruviana L. and Solanum muricatum Aiton via decoction and maceration methods was studied. The highest extraction yields were recorded by methanol for decoction and acetone for maceration. The total polyphenol content (TPC) obtained by decoction had the highest TPC contents, and MeOH containing NaF was the best solvent for the extraction of TPC. Maceration was suitable for flavonoid extractions, with ethanol and acetone being the best solvents. In general, the highest levels of TPC and flavonoids were obtained from Passiflora leaves regardless of the solvent or extraction method applied. Furthermore, the roots of Physalis and Solanum showed important levels of these compounds in consonance with the total antioxidant activity (TAA) evaluated in the different organs of the plant in the three species. In this study, the solvents and extraction methods applied were tools that determined significantly the level of extraction of bioactive compounds, showing a different impact on plant organs for each medicinal species studied.

scholarly journals Impact of Extraction Conditions on the Phenolic Composition and Antioxidant Capacity of Grape Stem Extracts

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 597 ◽  
Author(s):  
Nerea Jiménez-Moreno ◽  
Francesca Volpe ◽  
Jose Antonio Moler ◽  
Irene Esparza ◽  
Carmen Ancín-Azpilicueta
Keyword(s):  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Bioactive Compounds ◽  
Extraction Method ◽  
Ethanol Concentration ◽  
Total Phenolic ◽  
Phenolic Composition ◽  
Total Phenolic Compounds ◽  
Extraction Solvent ◽  
Selection Of

The use of grape stems for the extraction of bioactive compounds to be used in the pharmaceutical, food, and cosmetic industries is a promising objective. The aim of this work is to determine the influence of the different extraction conditions (temperature, ethanol concentration, and ratio of sample/solvent) on phenolic composition and antioxidant capacity of Mazuelo stem extracts. In general, the ethanol concentration of the extraction solvent was the factor that had the greatest influence on the extraction of different bioactive compounds. The greatest content of total phenolic compounds and the highest antioxidant activity of the extracts were obtained with 50% ethanol and at 40 °C. The most abundant compound found in the different extracts obtained from Mazuelo grape stem was (+)-catechin, but appreciable concentrations of gallic acid, a quercetin derivative, and stilbenes (trans-resveratrol and trans-ε-viniferin) were also extracted. Quercetin and malvidin-3-glucoside showed the highest correlation with the antioxidant capacity of the extracts, while stilbenes did not present such relation. The maximum concentration of gallic acid was extracted with water but the extraction of most of the compounds was maximum on using 50% ethanol. Consequently, the selection of the extraction method to be used will depend on the particular compound to be extracted in greatest quantity.

scholarly journals Nutrients and bioactive compounds of pulp, peel and seed from umbu fruit

2019 ◽  
Vol 49 (4) ◽  
Author(s):  
Leilson de Oliveira Ribeiro ◽  
Eliseth de Souza Viana ◽  
Ronoel Luiz de Oliveira Godoy ◽  
Sidinea Cordeiro de Freitas ◽  
Suely Pereira Freitas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Nutritional Composition ◽  
Total Phenolic ◽  
Total Carotenoids ◽  
Fresh Pulp ◽  
Main Barrier ◽  
By Products

ABSTRACT: The objective of this research was to evaluate the nutritional composition and bioactive compounds of whole umbu fruit, including pulp, seed and peel, and also of a commercial umbu pulp. Samples of the fractions and of commercial pulp were analyzed for determination of minerals and proximate composition, total phenolic and antioxidant activity. Pulps and peel were also analyzed for vitamin C and carotenoids contents. Commercial pulp presented better nutritional composition than fresh pulp (P<0.05) and the peel presented higher phenolic content and antioxidant activity than seed. Peel also stood out by its vitamin C (79 mg.100 g-1) and total carotenoids (2,751 µg.100 g-1) contents, showing that, as the main barrier of the fruit for its protection, it is a fraction rich in bioactive compounds. The highest dietary fiber and iron contents were observed in umbu seed (P<0.05). Therefore, umbu by-products may be ingredients proper for development of food richer in nutrients and bioactive compounds.

scholarly journals PERBANDINGAN METODE EKSTRAKSI EKSTRAK UMBI BAWANG RAMBUT (Allium chinense G.Don.) MENGGUNAKAN PELARUT ETANOL 70% TERHADAP RENDEMEN DAN SKRINING FITOKIMIA

2019 ◽  
Vol 1 (1) ◽  
pp. 30-40
Author(s):  
Supomo Supomo ◽  
Husnul Warnida ◽  
Bagus Moch Said
Keyword(s):  
Experimental Study ◽  
Statistical Analysis ◽  
Secondary Metabolite ◽  
Bioactive Compounds ◽  
Extraction Method ◽  
Extraction Methods ◽  
Phytochemical Screening ◽  
Onion Bulbs ◽  
East Kalimantan ◽  
Allium Chinense

Allium chinense are plants that contain bioactive compounds such as alkaloids, flavonoids, and saponins. The rendement of an extract may be affected by the extraction mentod used. This study aims to determine the effect of maseration and digestion extraction method on rendemen and identification of secondary metabolite in the extract of the hair onion bulbs. Research conducted is an experimental study.  The samples used were hair bulbs obtained in Kota Bangun, East Kalimantan, extracted wiyh 2 extraction methods of maceration and digestion using ethanol 70% solvent and done as much as 3 times replication. Identification of secondary metabolite compounds was performed by phytochemical screening including test of alkaloids, flavonoids, saponins, tannins, and steroids. From the test data is processed by statistical analysis. The result of rendement with 3 times replikation of digesti mentod obtained by 20,02 gram, 19,03 gram, and 19,17 gram. While the maseration method obtained for 12,38 grams, 12,45 grams, and 15,91 grams. So there are differences between the results of the results of digestion mentod and maseration mentod. Based on phytochemical screening tets showed that the thick extract of hair onion bulbs contain compounds alkaloids, flavonoids, and saponins.

scholarly journals Effects of Extraction Methods and Conditions on Bioactive Compounds Extracted from Phaeodactylum tricornutum

2020 ◽  
Vol 67 (4) ◽  
pp. 1250-1261
Author(s):  
Saniye Akyıl ◽  
Işıl İlter ◽  
Mehmet Koç ◽  
Zeliha Demirel ◽  
Ayşegül Erdoğan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Extraction Method ◽  
Phaeodactylum Tricornutum ◽  
Phenolic Content ◽  
Extraction Methods ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Microwave Extraction

The effect of homogenization, ultrasound and microwave extraction methods and conditions on fucoxanthin content, total phenolic content and antioxidant activity of extracts obtained from Phaeodactylum tricornutum were investigated in this study. The solvent/biomass ratio was the most effective parameter on fucoxanthin content, total phenolic content and antioxidant activity. The maximum fucoxanthin content (5.60 ± 0.06 mg/g) and antioxidant activity (763.00 ± 15.88 EC50 μg/mL extract) were obtained with the homogenization extraction method whose optimum conditions were 1.93% biomass/solvent ratio, ~5200 rpm homogenization rate and 14.2 min extraction time. Although the ultrasonic extraction method has reached the approximately same level of fucoxanthin content (5.24 ± 0.07 mg/g)), TPC (67.68 ± 1.58 mg gallic acid/L) and antioxidant activity (619.90 ± 17.16 EC50 μg/mL extract) at an amplitude of 55.72%, a higher biomass/solvent ratio (2.72%) and a longer extraction time (17.37 min) have been required. The lowest fucoxanthin content, total phenolic content and antioxidant activity were determined for the microwave extraction method.

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