Evaluation of Antimicrobial and Anticancer Activities of Selected Medicinal Plants of Himalayas, Pakistan

Medicinal plants are known for their diverse use in the traditional medicine of the Himalayan region of Pakistan. The present study is designed to investigate the anticancer and antimicrobial activities of Prunus cornuta and Quercus semicarpifolia. The anticancer activity was performed using cancerous human cell lines (HepG2, Caco-2, A549, MDA-MB-231, and NCI-H1437 carcinoma cells), while the antimicrobial activity was conducted with the agar-well diffusion method. Furthermore, toxicity studies were performed on alveolar and renal primary epithelial cells. Initially, different extracts were prepared by maceration techniques using n-hexane, chloroform, ethyl acetate, butanol, and methanol. The preliminary phytochemical screening showed the presence of secondary metabolites such as alkaloids, tannins, saponins, flavonoids, glycosides, and quinones. The chloroform extract of P. cornuta (PCC) exhibited significant inhibitory activity against Acinetobacter baumannii (16 mm) and Salmonella enterica (14.5 mm). The A. baumannii and S. enterica strains appeared highly susceptible to n-hexane extract of P. cornuta (PCN) with an antibacterial effect of 15 mm and 15.5 mm, respectively. The results also showed that the methanolic extracts of Quercus semecarpifolia (QSM) exhibited considerable antibacterial inhibitory activity in A. baumannii (18 mm), Escherichia coli (15 mm). The QSN and QSE extracts also showed good inhibition in A. baumannii with a 16 mm zone of inhibition. The Rhizopus oryzae strain has shown remarkable mycelial inhibition by PCM and QSN with 16 mm and 21 mm inhibition, respectively. Furthermore, the extracts of P. cornuta and Q. semicarpifolia exhibited prominent growth inhibition of breast (MDA-MB-231) and lung (A549) carcinoma cells with 19–30% and 22–39% cell viabilities, respectively. The gut cell line survival was also significantly inhibited by Q. semicarpifolia (24–34%). The findings of this study provide valuable information for the future development of new antibacterial and anticancer medicinal agents from P. cornuta and Q. semicarpifolia extracts.

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Efficacy of Kumaun Himalayan Biota orientalis Endl. leaves extracts against pathogenic fungi

International Journal of Pharmacology and Toxicology ◽

10.14419/ijpt.v4i2.6702 ◽

2016 ◽

Vol 4 (2) ◽

pp. 224

Author(s):

Savita Joshi ◽

S. C. Sati ◽

Parikshit Kumar

Keyword(s):

Inhibitory Activity ◽

Ethanol Extract ◽

Pathogenic Fungi ◽

Chloroform Extract ◽

Positive Control ◽

Diffusion Method ◽

Colletotrichum Falcatum ◽

Fungal Strains ◽

Percent Inhibition ◽

Biota Orientalis

An increasing demand for natural plant products has shifted the attention from synthetic to natural antifungal agents. This study was carried out to evaluate the antifungal activity of methanol, ethanol, chloroform, hexane and water extracts of Biota orientalis Endl. leaves, a Kumaun Himalayan gymnospermic plant. The antifungal potential of all extracts of B. orientalis were tested against seven different fungal strains (Alternaria alternata, Colletotrichum falcatum, Fusarium oxysporum, Pyricularia oryzae, Sclerotinia rolfsii, Sclerotinia sclerotiorum and Tilletia indica) using agar-well diffusion method. The ethanol extract was found most active against all the pathogens tested (Percent inhibition, 27-59%) followed by hexane extract (Percent inhibition, 31-58%) and methanol extract (27-57%) while chloroform and aqueous extracts were found totally inactive against all the tested fungal strains, only chloroform extract showed inhibitory activity against S. rolfsii (% inhibition, 58%). The inhibitory activity of these extracts was found very effective as compared to Clotrimazol, standard antifungal agent that was used as positive control against tested fungal strains.

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Evaluation of antioxidant and antimicrobial activities of chloroformic and methanolic extracts of 6 important medicinal plants collected from North of Iran

10.21203/rs.2.19337/v2 ◽

2020 ◽

Author(s):

Somayeh Ghahari ◽

Zahra Hadadi ◽

Ghorban Ali Nematzadeh

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Radical Scavenging ◽

Antimicrobial Activities ◽

Bioactive Molecules ◽

Diffusion Method ◽

Total Phenolic ◽

Guaiacol Peroxidase ◽

Phytochemical Analysis ◽

Methanolic Extracts

Abstract Background: as possible sources of natural bioactive molecules, the plant essential oils and extracts have been used globally in new antimicrobial compounds, food preservatives, and alternatives to treat infectious disease.Methods: In this research, the antimicrobial activities of chloroformic and methanolic extracts of Sophora flavescens, Rhaponticum repens, Alhagi maurorum, Melia azedarach, Peganum harmala, and Juncus conglomeratus were evaluated against 8 bacteria (S. aureus, B. subtilis, R. toxicus, P. aeruginosa, E. coli, P. syringae, X. campestris, P. viridiflava) and 3 fungi (Pyricularia oryzae, Fusarium oxysporum and Botrytis cinerea), through disc diffusion method. Furthermore, the essential oils of plants with the highest antibacterial activity were analyzed utilizing GC/MS. Moreover, the tested plants were exposed to screening for possible antioxidant effect utilizing DPPH test, guaiacol peroxidas, and catalase enzymes. Besides, the amount of total phenol and flavonoid of these plants was measured. Results: Among the tested plants, methanolic and chloroformic extracts of P. harmala fruits showed the highest antibacterial activity against the tested bacteria. Besides, the investigation of free radical scavenging effects of the tested plants indicated the highest DPPH, protein, guaiacol peroxidase, and catalase in P. harmala, M. azedarach, J. conglomeratus fruits, and J. conglomeratus fruits, respectively. In addition, the phytochemical analysis demonstrated the greatest amounts of total phenolic and flavonoid compositions in J. conglomeratus and P. harmala, respectively.Conclusion: The results indicated that these plants could act as a promising antimicrobial agent, due to their short killing time.

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Preliminary phytochemical screening and antimicrobial activities of plant extract of Elaeocarpus ganitrus Roxb.

International Journal of Bioassays ◽

10.21746/ijbio.2016.09.0019 ◽

2016 ◽

Vol 5 (09) ◽

pp. 4885 ◽

Cited By ~ 1

Author(s):

Khushbu Pandey ◽

Mahendra Singh* ◽

Bharat Pandey ◽

Anshulika Upadhyaya ◽

Kamal K. Pande

Keyword(s):

Antimicrobial Activity ◽

Rhizopus Oryzae ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Bacterial Strains ◽

E Coli ◽

Antibacterial And Antifungal ◽

Anti Fungal

The present study was carried out for phytochemical screening of principle bioactive compounds and antimicrobial activity in Elaeocarpus ganitrus Roxb., Phytochemical analysis revealed the presence of saponin, terpenoid, steroid, saponin, flavonoid, tannin and alkaloid. The petroleum, ether, chloroform, methanol, acetone and aqueous extracts were subjected to antimicrobial activity against bacterial strains Staphylococcus aureus, Pseudomonas, E. coli and Bacillus subtilis against anti-fungal strains A.awamori, A.fumigatus, Rhizopus oryzae, Trichoderma viridae and C.oryzae. The antibacterial and antifungal activity was evaluated by disc-diffusion method.

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Evaluation of antioxidant and antimicrobial activities of chloroformic and methanolic extracts of 6 important medicinal plants collected from Northern of Iran

10.21203/rs.2.19337/v1 ◽

2019 ◽

Author(s):

Somayeh Ghahari ◽

Zahra Hadadi ◽

Ghorban Ali Nematzadeh

Keyword(s):

Antibacterial Activity ◽

Radical Scavenging ◽

Antimicrobial Activities ◽

Bioactive Molecules ◽

Diffusion Method ◽

Melia Azedarach ◽

Total Phenolic ◽

Guaiacol Peroxidase ◽

Phytochemical Analysis ◽

Methanolic Extracts

Abstract Background: The plant essential oils and extracts are possible sources of natural bioactive molecules and have been selected globally for new antimicrobial compounds, food preservatives, and alternatives to treat infectious disease.Methods: In this research, antimicrobial activities of chloroformic and methanolic extracts of Sophora flavescens, Rhaponticum repens, Alhagi maurorum, Melia azedarach, Peganum harmala, and Juncus conglomeratus were evaluated against 8 bacteria (S. aureus, B. subtilis, R. toxicus, P. aeruginosa, E. coli, P. syringae, X. campestris, P. viridiflava) and 3 fungi (Pyricularia oryzae, Fusarium oxysporum and Botrytis cinerea) through disc diffusion method. Furthermore, the essential oil of plants with the highest antibacterial activity was analyzed utilizing GC/MS. Moreover, tested plants were exposed to screening for possible antioxidant effect utilizing DPPH test, guaiacol peroxidas, and catalase enzymes. Besides, the amount of total phenol and flavonoid of these plants was measured. Results: Among tested plants, methanolic and chloroformic extracts of P. harmala fruits showed the highest antibacterial activity against tested bacteria. Also, the investigation of free radical scavenging effects of the tested plants indicated the highest DPPH, protein, guaiacol peroxidase, and catalase in P. harmala, M. azedarach, J. conglomeratus fruits, and J. conglomeratus fruits, respectively. In addition, the phytochemical analysis demonstrated the greatest amounts of total phenolic, and flavonoid compositions in J. conglomeratus and P. harmala. Conclusion: The results indicated that these plants can act as a promising antimicrobial agent due to its short killing time.

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FACILE SYNTHESIS, SPECTRAL STUDIES, DFT CALCULATIONS AND BIOLOGICAL ACTIVITIES OF NOVEL NI (II), CU (II), AND PD (II) COMPLEXES OF THIADIAZOLE ANALOGS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i4.17130 ◽

2017 ◽

Vol 9 (4) ◽

pp. 185

Author(s):

Priyanka Bhatt ◽

S. Deepthi ◽

Ch. Ravi Shankar Kumar ◽

Anjali Jha

Keyword(s):

Conventional Method ◽

Biological Activities ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Spectral Studies ◽

Spectroscopic Techniques ◽

Anticancer Activities ◽

Facile Synthesis ◽

Spectral Techniques ◽

Antibacterial And Antifungal Activities

Objective: A facile synthesis of some novel Schiff base derivatives of 2-substituted-5-amino-thiadiazoles along with their Ni (II), Cu (II), and Pd (II) complexes were achieved by sonication and the conventional method. In addition to establish the structure by DFT studies and to explore antimicrobial and anticancer activities of these novel compounds.Methods: The precursor 2-substituted-5-amino-thiadiazoles (T1-T3), target ligands and their metal complexes were synthesized by ultra-sonication and conventional means. The isolated products were thoroughly characterized by physical and spectroscopic techniques including 1H-NMR, [13]C-NMR and IR spectroscopy. All characterized compounds were screened for antimicrobial activities using well diffusion method, and MTT assay was performed for cytotoxicity.Results: All novel compounds were synthesized by a green route i.e. ultra sonication and a noticeable improvement in yield with shorter reaction time than the conventional method were observed. The octahedral geometry was proposed for Ni (II)/Cu (II) complexes whereas square planar for Pd (II) complexes on the basis of the spectral techniques which were supported by DFT analysis by Gaussian03. On the analysis of antimicrobial activities, the compound T7 and T10 showed maximum antibacterial and antifungal activities respectively. However, compounds T25, T37, T31 found to be a potential cytotoxic compound with IC50 value 0.469, 0.865 and 1.131 μM respectively.Conclusion: Analysis of synthetic protocol, it could be concluded that ultra-sonication is the better method to synthesize these potential biological active moiety. On the whole Cu (II) and Ni (II) complexes showed promising activity towards all microorganisms while Pd (II) complex emerged an excellent moiety in carcinoma cell line.

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Antimicrobial Activity of Selected Medicinal Plants from a Sub-Saharan African Country Against Bacterial Pathogens from Post-Operative Wound Infections

Medical Sciences ◽

10.3390/medsci9020023 ◽

2021 ◽

Vol 9 (2) ◽

pp. 23

Author(s):

Enid Owusu ◽

Martin Mensah Ahorlu ◽

Emmanuel Afutu ◽

Amos Akumwena ◽

George Awuku Asare

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Medicinal Plants ◽

Traditional Medicine ◽

Chronic Wounds ◽

Bacterial Pathogens ◽

Antimicrobial Activities ◽

Aureus Strain ◽

Diffusion Method ◽

Phytochemical Analysis

Background: Globally, the application of medicinal plants in the management of acute and chronic wounds can be considered a common occurrence in most traditional medicine practices. In view of this, many plants in the tropical and subtropical regions have been screened for their wound-healing activities. Consequently, plants having antimicrobial activity against multidrug-resistant (MD-R) pathogens can be considered great assets. Therefore, this study evaluated ethanolic and aqueous extracts of five medicinal plants (Psidium guajava, Myrianthus arboreus, Alchornea cordifolia, Momordica charantia, and Justicia flava) for their antimicrobial activities against MD-R bacterial pathogens isolated from post-operative wounds; Methods: This involved the aqueous and ethanolic extraction of the selected medicinal plants. Preliminary phytochemical constituents of the plants were examined. The agar well diffusion method was then used to determine the antibacterial activity of the leaves against reference strains (Escherichia coli ATCC 25922, Salmonella typhi ATCC 19430, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923, and a Methicillin-Resistant Staphylococcus aureus strain) as well as the MDR clinical isolates (E. coli, P. aeruginosa, S. aureus and CoNS) from the wounds; Results: The preliminary phytochemical analysis of the leaves showed the presence of saponins, phenolics, and reducing sugars in almost all the plants tested. All plant extracts were observed to have some antimicrobial activity against at least one reference strain. For the clinical MDR isolates, A. cordifolia from this study showed highest inhibition to growth of all bacteria used. Activity of J. flava against S. aureus was highest as compared to that of E.coli and P. aeruginosa. Similar observation was made for M. arboreus, P. guajava and M. charantia where the highest activity was observed against S. aureus; Conclusion: This study has mainly shown that P. guajava, M. arboreus, A. cordifolia, M. charantia, and J. flava exhibits antimicrobial activities against MD-R bacterial pathogens isolated from post-operative wounds. Also, these plants has bioactive phytochemical compounds with potential medicinal values for the treatment of numerous infections. Therefore, these plants may be helpful in the management of acute and chronic wounds, especially in traditional medicine practices.

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Evaluation of Phytochemical, Antimicrobial and Antioxidant Capacities of Pennisetum purpureum (Schumach) Extracts

Chemical Science International Journal ◽

10.9734/csji/2020/v29i430170 ◽

2020 ◽

pp. 1-14

Author(s):

I. R. Jack ◽

P. D. Clark ◽

G. I. Ndukwe

Keyword(s):

Antifungal Activity ◽

Vitamin C ◽

Methanolic Extract ◽

Antioxidant Activities ◽

Biological Activities ◽

Antimicrobial Activities ◽

Pennisetum Purpureum ◽

Diffusion Method ◽

Methanolic Extracts ◽

Test Organisms

Medicinal plants are valuable natural sources for the development of potentially safe drugs. The biological activities associated with these plants are due to the presence of certain phytochemicals that act individually or synergistically. Thus, this study examined the phytochemical components, antioxidant and antimicrobial activities of n-hexane, ethylacetate and methanolic extracts of Pennisetum purpureum (Schumach). Qualitative and quantitative phytochemical assays of P. purpureum showed the presence of alkaloids (0.004%), saponins (0.002%), flavonoids (0.021%), steroids, terpenoids and glycosides (0.008%). Methanol, n-hexane and ethylacetate extracts of P. purpureum were examined for antimicrobial activity using the disc diffusion method. Six microbial strains were exposed to six different concentrations of each extracts; 200 mg/ml, 100 mg/ml, 50 mg/ml, 25 mg/ml, 12.5 mg/ml and 6.25 mg/ml. The three extracts demonstrated varied concentration-dependent antimicrobial activities against the test organisms. The methanolic extract showed antibacterial activity against E. coli, S. aureus, B. cereus and antifungal activity against T. mentagrophyte and A. niger. Among all extracts, the methanolic extract of P. purpureum exhibited relatively strong antifungal activity against A. niger (10.3±0.12 mm) when compared to the standard antifungal agent, fluconazole (13.9±0.12 mm). Furthermore, antioxidant activities were spectrophotometrically studied using vitamin C as standard; methanol and ethylacetate extracts of P. purpureum showed pronounced scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH); and had a potent reductive ability on ferric ion and phosphomolybdate. However, only the non-polar extract of P. purpureum showed a non-significant correlation and significant differences when compared to vitamin C. Antioxidant activities of the plant extracts were observed in the order of methanol >ethylacetate> n-hexane extracts. The results showed that P. purpureum contains phytochemicals that significantly contributed to the observed antimicrobial and antioxidant abilities of the plant and could be used as a potential source for the development of novel therapeutic drugs.

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Evaluation of the phytochemical composition and antimicrobial properties of two aromatic plants used in the production of sweet wort and tchapalo, two artisanal beverages from Côte d'Ivoire

International Journal of Biological and Chemical Sciences ◽

10.4314/ijbcs.v14i9.19 ◽

2021 ◽

Vol 14 (9) ◽

pp. 3215-3230

Author(s):

Dolourou Diarrassouba ◽

Solange Aka ◽

Karamoko Ouattara ◽

Issa Bagre ◽

Nanga Yesse Zinzindorf ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Aromatic Plants ◽

Adansonia Digitata ◽

Phytochemical Composition ◽

Methanolic Extracts ◽

Phytochemical Compounds ◽

Extraits Aqueux

The search for new antimicrobial molecules from natural sources is an opportunity to preserve food. The objective of this study is to evaluate the phytochemical composition and antimicrobial properties of the leaves of Adansonia digitata (Bombacaceae) and the bark of Grewia venusta (Tilaceae), two aromatic plants used in the production of sweet must and tchapalo. Phytochemical screening from the aqueous, decocted and methanolic extracts of these plants was performed using standard colorimetric methods. Antimicrobial activity was also evaluated on 21 strains using the well diffusion method. Minimum Inhibitory Concentrations (MIC) and Bactericides were determined respectively by the liquid macro-dilution and agar plating methods. Results showed the presence of tannins, triterpenoids, cardiotonic glycosides and saponins in these extracts. All extracts were active on Staphylococcus aureus ATCC25923 with inhibition diameters ranging from 10.33±1 to 20.3±1.5 mm. The methanolic extracts had the lowest MICs (12.5 mg/mL), with bactericidal and/or fungicidal activity on most of the microbial strains tested. These results confirm the antimicrobial activities of the phytochemical compounds of these plants that could play the role of stabilizer and preservative of sweet wort and tchapalo. La recherche de nouvelles molécules antimicrobiennes à partir de sources naturelles est une opportunité pour conserver les aliments. L’objectif de cette étude est d’évaluer la composition phytochimique et les propriétés antimicrobiennes des feuilles de Adansonia digitata (Bombacaceae) et des écorces de Grewia venusta (Tilaceae), deux plantes aromatiques utilisées dans la production du moût sucré et du tchapalo. Le criblage phytochimiques à partir des extraits aqueux, décoctés et méthanoliques de ces plantes a été réalisé selon les méthodes standards de colorimétries. L’activité antimicrobienne a été également évaluée sur 21 souches en utilisant la méthode de diffusion en puits. Les Concentrations Minimales Inhibitrices (CMI) et Bactéricides ont été déterminées respectivement par les méthodes de macro-dilution en milieu liquide et ensemencement sur milieu gélosé. Les résultats ont montré la présence de tannins, de triterpénoïdes, de cardiotoniques glycosides et de saponines dans ces extraits. Tous les extraits ont été actifs sur Staphylococcus aureus ATCC25923 avec des diamètres d’inhibition allant de 10,33±1 à 20,3± 1,5 mm. Les extraits méthanoliques ont présenté les CMI les plus faibles (12,5 mg/mL), avec une activité bactéricide et/ou fongicide sur la plupart des souches microbiennes testées. Ces résultats confirment les activités antimicrobiennes des composés phytochimiques de ces plantes qui pourraient jouer le rôle de stabilisant et conservateur du moût sucré et du tchapalo.

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Comparative Analysis of a few Indian Medicinal Plants for their Antimicrobial and Antioxidant Properties

International Journal of Scientific Research in Science Engineering and Technology ◽

10.32628/ijsrset20718 ◽

2020 ◽

pp. 73-79

Author(s):

Kiran Kumar D. J. ◽

Bhanupriya H. J.

Keyword(s):

Comparative Analysis ◽

Medicinal Plants ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Centella Asiatica ◽

Mentha Piperita ◽

Diffusion Method ◽

Methanolic Extracts ◽

Bauhinia Purpurea

Plant-derived substances have been used since antiquity for therapeutic and other purposes. The present study was undertaken to make a comparative analysis of antimicrobial and antioxidant properties of 8 medicinal plants, which are used in traditional medicine system. Methanolic extracts of Centella asiatica (leaves), Mentha piperita (leaves), Calotropis gigantean (leaves), Vitex nigundo (leaves), Bauhinia racemosus (leaves), Emblica officinalis (fruit), Bauhinia purpurea (leaves), Asperagus racemosus (stem) were tested for anti-microbial effects using the standard Agar Diffusion method. Antioxidant attributes of above extracts were studied by testing their ability to scavenge DPPH and ABTS radicles. Our results indicated that all tested extracts, with the exception of Bauhinia purpurea, were effective against E. Coli, while Candida albicans was only sensitive to Asperagus racemosus. Among tested extracts, Bauhinia racemosus was most potent in exhibiting radical scavenging activity. Our data provides rationale for exploitation of these plants for antimicrobial and antioxidant attributes.

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Antimicrobial activity of three medicinal plants against acne-inducing bacteria Propionibacterium acnes

Brazilian Journal of Biological Sciences ◽

10.21472/bjbs.051008 ◽

2018 ◽

Vol 5 (10) ◽

pp. 277-288

Author(s):

S. E. Abiya ◽

B. O. Odiyi ◽

L. R. Falarunu ◽

N. U. Abiya

Keyword(s):

Medicinal Plants ◽

Propionibacterium Acnes ◽

Acne Vulgaris ◽

Aloe Vera ◽

Antimicrobial Activities ◽

Inhibitory Effect ◽

Diffusion Method ◽

Zone Of Inhibition ◽

Zones Of Inhibition ◽

Synthetic Standard

The antimicrobial activities of three medicinal plants (Senna alata L., Azadirachta indica A. Juss, and Aloe vera (L.) Burm.f.) against pimples causing bacterium Propionibacterium acnes was studied using disc diffusion method. Extracts from each plant were used on the bacterium at three different dosage concentrations (0.1 mL, 0.15 mL and 0.2 mL). Their Zone of inhibition was measured in millimeter (mm) and compared against a known synthetic standard (Gentamycin). Results indicate that the plants differ significantly in their activity against the studied microorganism. S. alata had the highest inhibitory effect of all the plants used (26.00 mm, 30.67 mm and 36.00 mm, for 0.1 mL, 0.15 mL and 0.2 mL dosage concentration, respectively). This was followed by A. indica with 9.33 mm, 15.67 mm and 16.67 mm zone of inhibition for 0.1 mL, 0.15 mL and 0.2 mL dosage concentration, respectively. A. vera had no effect (0.0 mm zone of inhibition) at 0.1 mL and 0.15 mL dosage concentrations, but at 2.0 mL dosage concentration, 4.0 mm zone of inhibition was achieved. Gentamycin showed zones of inhibition of 17.33 mm, 26.67 mm and 22.67 mm, for 0.1 mL, 0.15 mL and 0.2 mL dosage concentration, respectively. A comparison of all result obtained from the three plant extracts and gentamycin shows that S. alata have a significantly higher (p > 0.05) inhibitory effect against the pimples causing bacterium; Propionibacterium acnes than all the other treatments. The trend follows S. alata > Gentamycin > A. indica > A. vera, respectively, in terms of their inhibitory effect. Therefore, S. alata is more active and is the most appropriate plant to be used for treating of acne vulgaris among the three plant species selected for this experiment.

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