scholarly journals Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1930 ◽  
Author(s):  
Ming-You Shie ◽  
Jian-Jr Lee ◽  
Chia-Che Ho ◽  
Ssu-Yin Yen ◽  
Hooi Yee Ng ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physical Properties ◽  
Dermal Fibroblast ◽  
Sol Gel ◽  
Biological Properties ◽  
Human Dermal Fibroblast ◽  
Natural Material ◽  
Matrix Remodeling ◽  
Proliferation Markers

Gelatin-methacryloyl (GelMa) is a very versatile biomaterial widely used in various biomedical applications. The addition of methacryloyl makes it possible to have hydrogels with varying mechanical properties due to its photocuring characteristics. In addition, gelatin is obtained and derived from natural material; thus, it retains various cell-friendly motifs, such as arginine-glycine-aspartic acid, which then provides implanted cells with a friendly environment for proliferation and differentiation. In this study, we fabricated human dermal fibroblast cell (hDF)-laden photocurable GelMa hydrogels with varying physical properties (5%, 10%, and 15%) and assessed them for cellular responses and behavior, including cell spreading, proliferation, and the degree of extracellular matrix remodeling. Under similar photocuring conditions, lower concentrations of GelMa hydrogels had lower mechanical properties than higher concentrations. Furthermore, other properties, such as swelling and degradation, were compared in this study. In addition, our findings revealed that there were increased remodeling and proliferation markers in the 5% GelMa group, which had lower mechanical properties. However, it was important to note that cellular viabilities were not affected by the stiffness of the hydrogels. With this result in mind, we attempted to fabricate 5–15% GelMa scaffolds (20 × 20 × 3 mm3) to assess their feasibility for use in skin regeneration applications. The results showed that both 10% and 15% GelMa scaffolds could be fabricated easily at room temperature by adjusting several parameters, such as printing speed and extrusion pressure. However, since the sol-gel temperature of 5% GelMa was noted to be lower than its counterparts, 5% GelMa scaffolds had to be printed at low temperatures. In conclusion, GelMa once again was shown to be an ideal biomaterial for various tissue engineering applications due to its versatile mechanical and biological properties. This study showed the feasibility of GelMa in skin tissue engineering and its potential as an alternative for skin transplants.

Fabrication of Chitosan Nanofibers Scaffolds with Small Amount Gelatin for Enhanced Cell Viability

2015 ◽  
Vol 749 ◽  
pp. 220-224 ◽  
Author(s):  
Min Sup Kim ◽  
Sang Jun Park ◽  
Bon Kang Gu ◽  
Chun Ho Kim
Keyword(s):  
Mechanical Properties ◽  
Cell Culture ◽  
Cell Viability ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Biological Properties ◽  
Human Dermal Fibroblast ◽  
Initial Contact ◽  
Initial Contact Angle ◽  
Contact Angle Analysis

Chitosan and gelatin has attracted considerable interest owing to its advantageous biological properties such as excellent biocompatibility, biodegradation, and non-toxic properties. In this paper, we investigated the potential of chitosan/gelatin (Chi-Gel) nanofibers mat with enhanced cell viability for use as cell culture scaffolds. The surface morphology, mechanical properties, and initial contact angle analysis of Chi-Gel nanofibers mat were evaluated. The proliferation of human dermal fibroblast cell (HDFs) on Chi-Gel nanofibers mat was found to be approximately 20% higher than the pure chitosan nanofibers mat after 7 days of culture. These results suggest that the Chi-Gel nanofibers mat has great potential for use tissue engineering applications.

Crosslinked Gelatin Nanofibers and their Potential for Tissue Engineering

2007 ◽  
Vol 342-343 ◽  
pp. 169-172 ◽  
Author(s):  
Young Jin Kim ◽  
Oh Hyeong Kwon
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Dermal Fibroblast ◽  
Solvent System ◽  
Human Dermal Fibroblast ◽  
Deionized Water ◽  
Electrospun Gelatin

Gelatin nanofibers were obtained by the use of TFE/deionized water co-solvent system without adding any other fiber forming material. The diameter of nanofibers was in the rage from 200 to 360 nm. The water-resistant ability and mechanical properties of electrospun gelatin nanofibers were improved by crosslinking in GTA or FA vapor, which increased with crosslinking time. Cytotoxicity evaluation indicates that the crosslinked gelatin membranes support the proliferation of human dermal fibroblast.

Hybrid nanostructured hydroxyapatite–chitosan composite scaffold: bioinspired fabrication, mechanical properties and biological properties

2015 ◽  
Vol 3 (23) ◽  
pp. 4679-4689 ◽  
Author(s):  
Ya-Ping Guo ◽  
Jun-Jie Guan ◽  
Jun Yang ◽  
Yang Wang ◽  
Chang-Qing Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Biological Properties ◽  
Chitosan Composite

A bioinspired strategy has been developed to fabricate a hybrid nanostructured hydroxyapatite–chitosan composite scaffold for bone tissue engineering.

scholarly journals Additive manufacturing of PLA-based scaffolds intended for bone regeneration and strategies to improve their biological properties

e-Polymers ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 571-599
Author(s):  
Ricardo Donate ◽  
Mario Monzón ◽  
María Elena Alemán-Domínguez
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Additive Manufacturing ◽  
Bone Regeneration ◽  
Polylactic Acid ◽  
State Of The Art ◽  
Biological Properties ◽  
Design Stage ◽  
Bone Scaffolds ◽  
Regeneration Of Bone

AbstractPolylactic acid (PLA) is one of the most commonly used materials in the biomedical sector because of its processability, mechanical properties and biocompatibility. Among the different techniques that are feasible to process this biomaterial, additive manufacturing (AM) has gained attention recently, as it provides the possibility of tuning the design of the structures. This flexibility in the design stage allows the customization of the parts in order to optimize their use in the tissue engineering field. In the recent years, the application of PLA for the manufacture of bone scaffolds has been especially relevant, since numerous studies have proven the potential of this biomaterial for bone regeneration. This review contains a description of the specific requirements in the regeneration of bone and how the state of the art have tried to address them with different strategies to develop PLA-based scaffolds by AM techniques and with improved biofunctionality.

scholarly journals Porous Cellulose-Collagen Scaffolds For Soft Tissue Regeneration: Influence of Cellulose Derivatives On Mechanical Properties And Compatibility With Adipose-Derived Stem Cells.

2022 ◽  
Author(s):  
Katarína Kacvinská ◽  
Martina Trávničková ◽  
Lucy Vojtová ◽  
Petr Poláček ◽  
Jana Dorazilová ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Adhesion ◽  
Soft Tissue ◽  
Vascular Tissue ◽  
Biological Properties ◽  
Cellulose Derivatives ◽  
Adipose Derived Stem Cells ◽  
Soft Tissue Regeneration

Abstract This study deals with cellulose derivatives in relation to the collagen fibrils in composite collagen-cellulose scaffolds for soft tissue engineering. Two types of cellulose, i.e., oxidized cellulose (OC) and carboxymethyl cellulose (CMC), were blended with collagen (Col) to enhance its elasticity, stability and sorptive biological properties, e.g. hemostatic and antibacterial features. The addition of OC supported the resistivity of the Col fibrils in a dry environment, while in a moist environment OC caused a radical drop. The addition of CMC reduced the mechanical strength of the Col fibrils in both environments. The elongation of the Col fibrils was increased by both types of cellulose derivatives in both environments, which is closely related to tissue like behaviour. In these various mechanical environments, the ability of human adipose-derived stem cells (hADSCs) to adhere and proliferate was significantly greater in the Col and Col/OC scaffolds than in the Col/CMC scaffold. This is explained by deficient mechanical support and loss of stiffness due to the high swelling capacity of CMC. Although Col/OC and Col/CMC acted differently in terms of mechanical properties, both materials were observed to be cytocompatible, with varying degrees of further support for cell adhesion and proliferation. While Col/OC can serve as a scaffolding material for vascular tissue engineering and for skin tissue engineering, Col/CMC seems to be more suitable for moist wound healing, e.g. as a mucoadhesive gel for exudate removal, since there was almost no cell adhesion.

scholarly journals Suture Fiber Reinforcement of a 3D Printed Gelatin Scaffold for Its Potential Application in Soft Tissue Engineering

2021 ◽  
Vol 22 (21) ◽  
pp. 11600
Author(s):  
Dong Jin Choi ◽  
Kyoung Choi ◽  
Sang Jun Park ◽  
Young-Jin Kim ◽  
Seok Chung ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mechanical Strength ◽  
Physical Properties ◽  
Dimensional Stability ◽  
3D Structure ◽  
Biological Properties ◽  
Dermal Fibroblasts ◽  
3D Scaffolds ◽  
3D Printed

Gelatin has excellent biological properties, but its poor physical properties are a major obstacle to its use as a biomaterial ink. These disadvantages not only worsen the printability of gelatin biomaterial ink, but also reduce the dimensional stability of its 3D scaffolds and limit its application in the tissue engineering field. Herein, biodegradable suture fibers were added into a gelatin biomaterial ink to improve the printability, mechanical strength, and dimensional stability of the 3D printed scaffolds. The suture fiber reinforced gelatin 3D scaffolds were fabricated using the thermo-responsive properties of gelatin under optimized 3D printing conditions (−10 °C cryogenic plate, 40–80 kPa pneumatic pressure, and 9 mm/s printing speed), and were crosslinked using EDC/NHS to maintain their 3D structures. Scanning electron microscopy images revealed that the morphologies of the 3D printed scaffolds maintained their 3D structure after crosslinking. The addition of 0.5% (w/v) of suture fibers increased the printing accuracy of the 3D printed scaffolds to 97%. The suture fibers also increased the mechanical strength of the 3D printed scaffolds by up to 6-fold, and the degradation rate could be controlled by the suture fiber content. In in vitro cell studies, DNA assay results showed that human dermal fibroblasts’ proliferation rate of a 3D printed scaffold containing 0.5% suture fiber was 10% higher than that of a 3D printed scaffold without suture fibers after 14 days of culture. Interestingly, the supplement of suture fibers into gelatin biomaterial ink was able to minimize the cell-mediated contraction of the cell cultured 3D scaffolds over the cell culture period. These results show that advanced biomaterial inks can be developed by supplementing biodegradable fibers to improve the poor physical properties of natural polymer-based biomaterial inks.

scholarly journals Effects of Encapsulated Cells on the Physical–Mechanical Properties and Microstructure of Gelatin Methacrylate Hydrogels

2019 ◽  
Vol 20 (20) ◽  
pp. 5061 ◽  
Author(s):  
Srikumar Krishnamoorthy ◽  
Behnam Noorani ◽  
Changxue Xu
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Viability ◽  
Physical Properties ◽  
Pore Size ◽  
Cell Density ◽  
Tensile Modulus ◽  
Encapsulated Cells ◽  
3T3 Fibroblasts ◽  
Cell Densities

Gelatin methacrylate (GelMA) has been gaining popularity in recent years as a photo-crosslinkable biomaterial widely used in a variety of bioprinting and tissue engineering applications. Several studies have established the effects of process-based and material-based parameters on the physical–mechanical properties and microstructure of GelMA hydrogels. However, the effect of encapsulated cells on the physical–mechanical properties and microstructure of GelMA hydrogels has not been fully understood. In this study, 3T3 fibroblasts were encapsulated at different cell densities within the GelMA hydrogels and incubated over 96 h. The effects of encapsulated cells were investigated in terms of mechanical properties (tensile modulus and strength), physical properties (swelling and degradation), and microstructure (pore size). Cell viability was also evaluated to confirm that most cells were alive during the incubation. It was found that with an increase in cell density, the mechanical properties decreased, while the degradation and the pore size increased.

scholarly journals TiO2 doped chitosan/hydroxyapatite/halloysite nanotube membranes with enhanced mechanical properties and osteoblast-like cell response for application in bone tissue engineering

RSC Advances ◽  
2019 ◽  
Vol 9 (68) ◽  
pp. 39768-39779
Author(s):  
Sarim Khan ◽  
Viney Kumar ◽  
Partha Roy ◽  
Patit Paban Kundu
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Response ◽  
Halloysite Nanotubes ◽  
Biological Properties ◽  
Halloysite Nanotube ◽  
Nanotube Membranes ◽  
Hydroxyapatite Composite

This two-stage study aims to optimize the amount of halloysite nanotubes and TiO2 in a chitosan/nano-hydroxyapatite composite to tailor the mechanical and biological properties for application in bone tissue engineering.

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