scholarly journals A Green Process for the Extraction and Purification of Hesperidin from Mexican Lime Peel (Citrus aurantifolia Swingle) that is Extendible to the Citrus Genus

Processes ◽  
2018 ◽  
Vol 6 (12) ◽  
pp. 266 ◽  
Author(s):  
J. Padilla de la Rosa ◽  
Priscilla Ruiz-Palomino ◽  
Enrique Arriola-Guevara ◽  
Jorge García-Fajardo ◽  
Georgina Sandoval ◽  
...  
Keyword(s):  
Packed Column ◽  
Pilot Scale ◽  
Solvent Ratio ◽  
Recovery Efficiency ◽  
Purification Process ◽  
Green Process ◽  
Static Tests ◽  
Mexican Lime ◽  
Extraction And Purification ◽  
Lime Peel

The processing of Mexican limes generates great amounts of peel as a by-product. Lime peel is mainly rich in the flavonoid hesperidin, whose bioactivity is oriented mainly to cardiovascular diseases and cancer. The purpose of this work was to develop a green process for the extraction and purification of hesperidin from Mexican lime peel. The extraction of hesperidin was investigated on a laboratory scale by varying the solvent composition and the solid-to-solvent ratio, and then scaling this process (volume: 20 L). Next, a purification process using adsorption resins was assessed: first through static tests to determine the adsorption efficiency with two resins (FPX66, EXA118), and then on a packed column with 80 mL of resin at 25 °C. Lab-scale extraction showed that the best conditions were a solid-to-solvent ratio of 0.33 g/mL and 60% ethanol. After using these conditions at pilot scale and concentrating the solution, the hesperidin content of the extract was 0.303 mg/mL. Through static tests, higher adsorption efficiencies were achieved with the EXA-118 resin and diluted extract (4:6 ratio with 10% dimethylsulfoxide, (DMSO)). Finally, the purification process on a packed column from the diluted extract (hesperidin concentration of 0.109 mg/mL) had a mean recovery efficiency of almost 90%.

scholarly journals A Green Process for the Extraction and Purification of Hesperidin from Mexican Lime Peel (Citrus aurantifolia Swingle) Extendible to Citrus Genus

2018 ◽  
Author(s):  
José Daniel Padilla de la Rosa ◽  
Priscilla Ruiz-Palomino ◽  
Guadalupe M. Guatemala-Morales ◽  
Jorge A. García-Fajardo ◽  
Georgina C. Sandoval-Fabián ◽  
...  
Keyword(s):  
Pilot Scale ◽  
Solvent Ratio ◽  
Recovery Efficiency ◽  
Green Process ◽  
Static Tests ◽  
Mexican Lime ◽  
Extraction And Purification ◽  
The Mean ◽  
Lime Peel ◽  
Kinetics Of

The processing of Mexican limes generates great amounts of peel as a byproduct. Lime peel is mainly rich in the flavonoid hesperidin, whose bioactivity is oriented mainly to cardiovascular diseases and cancer. The purpose of this work was to develop a green process for the extraction and purification of hesperidin from Mexican lime peel. The extraction of hesperidin was investigated on a laboratory scale by varying the solvent composition and the solid-to-solvent ratio. The best conditions (solid-to-solvent ratio of 0.33 g/mL and 60% ethanol) were used for the extraction of hesperidin in a pilot scale (Volume = 20 L). The kinetics of the extraction was studied to find the maximum hesperidin concentration at 100 min. The concentrated extract had a hesperidin content of 0.303 mg/mL. Next, a purification process using adsorption resins was assessed. Through static tests, it was determined that higher adsorption efficiencies were achieved with the EXA-118 resin and diluted extract (4:6 ratio with 10% DMSO). Finally, the adsorption of hesperidin from the diluted extract (hesperidin concentration of 0.109 mg/mL) was carried out at 25 °C in a column packed with 80 mL of EXA-118 resin. The mean recovery efficiency of hesperidin from the extract was almost 90%.

scholarly journals Assessment of Long-Term Fermentability of PHA-Based Materials from Pure and Mixed Microbial Cultures for Potential Environmental Applications

Water ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 897
Author(s):  
Neda Amanat ◽  
Bruna Matturro ◽  
Marta Maria Rossi ◽  
Francesco Valentino ◽  
Marianna Villano ◽  
...  
Keyword(s):  
Environmental Remediation ◽  
Volatile Fatty Acids ◽  
Rapid Onset ◽  
Pilot Scale ◽  
Point Of View ◽  
Microbial Cultures ◽  
Extraction And Purification ◽  
Fermentation Pattern ◽  
Mixed Microbial Cultures

The use of polyhydroxyalkanoates (PHA) as slow-release electron donors for environmental remediation represents a novel and appealing application that is attracting considerable attention in the scientific community. In this context, here, the fermentation pattern of different types of PHA-based materials has been investigated in batch and continuous-flow experiments. Along with commercially available materials, produced from axenic microbial cultures, PHA produced at pilot scale by mixed microbial cultures (MMC) using waste feedstock have been also tested. As a main finding, a rapid onset of volatile fatty acids (VFA) production was observed with a low-purity MMC-deriving material, consisting of microbial cells containing 56% (on weight basis) of intracellular PHA. Indeed, with this material a sustained, long-term production of organic acids (i.e., acetic, propionic, and butyric acids) was observed. In addition, the obtained yield of conversion into acids (up to 70% gVFA/gPHA) was higher than that obtained with the other tested materials, made of extracted and purified PHA. These results clearly suggest the possibility to directly use the PHA-rich cells deriving from the MMC production process, with no need of extraction and purification procedures, as a sustainable and effective carbon source bringing remarkable advantages from an economic and environmental point of view.

Analysis of Liquid Distribution in a Packed Column on a Pilot Scale

1998 ◽  
Vol 37 (7) ◽  
pp. 2844-2849 ◽  
Author(s):  
S. M. Pizzo ◽  
D. Moraes ◽  
F. A. N. Fernandes ◽  
M. S. Kobayasi ◽  
R. J. Pazini
Keyword(s):  
Packed Column ◽  
Pilot Scale ◽  
Liquid Distribution

scholarly journals Desorption of Organic Micropollutants from Loaded Granular Activated Carbon

Water ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2754
Author(s):  
Daniela Reif ◽  
Ernis Saracevic ◽  
Monika Šabić Runjavec ◽  
Julia Haslinger ◽  
Heidemarie Schaar ◽  
...  
Keyword(s):  
Particle Size ◽  
Activated Carbon ◽  
Contact Time ◽  
Granular Activated Carbon ◽  
Pilot Scale ◽  
Feed Water ◽  
Drying Methods ◽  
Solvent Ratio ◽  
Organic Micropollutants ◽  
The Impact

The loading of granular activated carbon (GAC) is influenced by the amount of water treated and the concentrations of adsorbates present in the water matrix. Through extraction experiments, we aimed to investigate the total adsorbed mass of eight organic micropollutants by using ethanol as solvent and the maximum possible concentrations, due to the desorption of organic micropollutants, in water. Three different drying methods and the impact of the contact time, GAC particle size, and GAC/solvent ratio were investigated. Although no significant differences between the drying methods could be observed, the chosen contact time and particle size had a significant impact on the amount of organic micropollutants extracted. Lower GAC/solvent ratios positively affected the extraction yield. The masses extracted in ethanol were compared with the cumulated masses calculated from 72 feed and effluent samples, collected during filter operation, resulting in extraction yields between 0.5% and 30%. The composition of extracted micropollutants in ethanol reflected the concentrations in feed water of the pilot-scale filter. Desorption in water was mostly influenced by the solubility of the investigated micropollutants. The same substances found in the supernatants inf the experiments could also be identified in the backwash water of the filter.

scholarly journals Systematic and Model-Assisted Process Design for the Extraction and Purification of Artemisinin from Artemisia annua L.—Part III: Chromatographic Purification

Processes ◽  
2018 ◽  
Vol 6 (10) ◽  
pp. 180 ◽  
Author(s):  
Fabian Mestmäcker ◽  
Axel Schmidt ◽  
Maximilian Huter ◽  
Maximilian Sixt ◽  
Jochen Strube
Keyword(s):  
High Performance ◽  
Artemisia Annua ◽  
Batch Process ◽  
Pilot Scale ◽  
Hplc Method ◽  
Artemisia Annua L ◽  
Reproducibility Study ◽  
Extraction And Purification ◽  
Layer Chromatography ◽  
Characteristic Process

In this study, the purification of an extract from Artemisia annua L. using chromatographic methods is studied. In a first step, a screening of different phases and solvents using thin-layer chromatography (TLC) was performed. Then, a laboratory-scale high performance liquid chromatography (HPLC) method was developed and transferred to a pilot scale. A reproducibility study based on 120 injections was carried out. The batch process that was developed and the results from a designed continuous simulated moving bed (SMB) chromatography were compared based on characteristic process numbers and economy.

scholarly journals Microwave-assisted extraction of phenolic compounds from Moringa oleifera seed as anti-biofouling agents in membrane processes

2018 ◽  
Vol 204 ◽  
pp. 03003 ◽  
Author(s):  
Ni'matul Izza ◽  
Shinta Rosalia Dewi ◽  
Ashried Setyanda ◽  
Agung Sukoyo ◽  
Panggulu Utoro ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Phenolic Content ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Purification Process ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Moringa Oleifera Seed

Moringa seed has known as a coagulant in the water purification process. It is because of the large amount of anti-microbial compounds contained in it. Phenol is one of the most common anti-microbial compounds found in natural materials. The aim of this study was to determine the total phenolic content (TPC) of Moringa seed which was extracted by Microwave-Assisted Extraction (MAE). Moringa seeds were characterized by FTIR and showed that it contained phenol compounds confirmed by specific peak in some areas. Moringa seeds were characterized by FTIR before extracted. Therefore, MAE was performed by variation of solvent ratios (1: 4, 1: 6, 1: 8) and extraction time (2, 3, 4 min). The highest TPC of 41.78 mg GAE / g dw was reached at 1: 8 solvent ratio and 3-min extraction time.

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