scholarly journals Plasma Agriculture from Laboratory to Farm: A Review

Processes ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 1002 ◽  
Author(s):  
Pankaj Attri ◽  
Kenji Ishikawa ◽  
Takamasa Okumura ◽  
Kazunori Koga ◽  
Masaharu Shiratani
Keyword(s):  
Seed Germination ◽  
Thermal Plasma ◽  
Biochemical Analysis ◽  
Early Stage ◽  
Growth Enhancement ◽  
Treated Water ◽  
Post Harvest ◽  
Non Thermal Plasma ◽  
Germination And Growth

In recent years, non-thermal plasma (NTP) application in agriculture is rapidly increasing. Many published articles and reviews in the literature are focus on the post-harvest use of plasma in agriculture. However, the pre-harvest application of plasma still in its early stage. Therefore, in this review, we covered the effect of NTP and plasma-treated water (PTW) on seed germination and growth enhancement. Further, we will discuss the change in biochemical analysis, e.g., the variation in phytohormones, phytochemicals, and antioxidant levels of seeds after treatment with NTP and PTW. Lastly, we will address the possibility of using plasma in the actual agriculture field and prospects of this technology.

scholarly journals Effects of Non-Thermal Plasma Treatment on Seed Germination and Early Growth of Leguminous Plants—A Review

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1616
Author(s):  
Božena Šerá ◽  
Vladimír Scholtz ◽  
Jana Jirešová ◽  
Josef Khun ◽  
Jaroslav Julák ◽  
...  
Keyword(s):  
Seed Germination ◽  
Plant Species ◽  
Thermal Plasma ◽  
Microbial Inactivation ◽  
Growth Stages ◽  
Agricultural Crop ◽  
Initial Growth ◽  
Total Production ◽  
Legume Seeds ◽  
Non Thermal Plasma

The legumes (Fabaceae family) are the second most important agricultural crop, both in terms of harvested area and total production. They are an important source of vegetable proteins and oils for human consumption. Non-thermal plasma (NTP) treatment is a new and effective method in surface microbial inactivation and seed stimulation useable in the agricultural and food industries. This review summarizes current information about characteristics of legume seeds and adult plants after NTP treatment in relation to the seed germination and seedling initial growth, surface microbial decontamination, seed wettability and metabolic activity in different plant growth stages. The information about 19 plant species in relation to the NTP treatment is summarized. Some important plant species as soybean (Glycine max), bean (Phaseolus vulgaris), mung bean (Vigna radiata), black gram (V. mungo), pea (Pisum sativum), lentil (Lens culinaris), peanut (Arachis hypogaea), alfalfa (Medicago sativa), and chickpea (Cicer aruetinum) are discussed. Likevise, some less common plant species i.g. blue lupine (Lupinus angustifolius), Egyptian clover (Trifolium alexandrinum), fenugreek (Trigonella foenum-graecum), and mimosa (Mimosa pudica, M. caesalpiniafolia) are mentioned too. Possible promising trends in the use of plasma as a seed pre-packaging technique, a reduction in phytotoxic diseases transmitted by seeds and the effect on reducing dormancy of hard seeds are also pointed out.

scholarly journals Non-Thermal Plasma Can Be Used in Disinfection of Scots Pine (Pinus sylvestris L.) Seeds Infected with Fusarium oxysporum

Forests ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 837
Author(s):  
Magdalena Świecimska ◽  
Mirela Tulik ◽  
Božena Šerá ◽  
Patrycja Golińska ◽  
Juliána Tomeková ◽  
...  
Keyword(s):  
Seed Germination ◽  
Pinus Sylvestris ◽  
Plasma Treatment ◽  
Fusarium Oxysporum ◽  
Scots Pine ◽  
Thermal Plasma ◽  
Optimal Time ◽  
Surface Barrier ◽  
Surface Barrier Discharge ◽  
Non Thermal Plasma

The aim of this study was to use diffuse coplanar surface barrier discharge (DCSBD) non-thermal plasma for the disinfection of pine seed surfaces infected with Fusarium oxysporum spores. Artificially infected seeds of Scots pine (Pinus sylvestris L.) were treated with plasma for the following exposure times: 1 s, 3 s, 5 s, 10 s, 15 s, 20 s, 30 s, and 60 s, and subsequently germinated on agar medium in Petri dishes at room temperature for the estimation of seed germination and disinfection effect of plasma treatment. Results of the treated samples were compared to the control samples, which were prepared as follows: seeds uninfected and non-treated with plasma (first control); seeds infected with F. oxysporum and non-treated with plasma (second control); and seeds infected with F. oxysporum, non-treated with plasma, but sterilized with 30% perhydrol (third control). Obtained results indicate that 3 s plasma treatment was an optimal time to inhibit F. oxysporum growth, and at the same time, increase the seed germination. In addition, our results are the first to show the practical application of non-thermal plasma in disinfecting infected Scots pine seeds and improving their germination. According to the results of this study, non-thermal plasma can serve as a seed surface disinfectant in the regeneration of different pine species.

Portable Plasma Biomedical Device for Cancer Treatment

2011 ◽  
Author(s):  
Magesh Thiyagarajan
Keyword(s):  
Thermal Plasma ◽  
Early Stage ◽  
Fluorescent Imaging ◽  
Acute Monocytic Leukemia ◽  
Air Plasma ◽  
Monocytic Leukemia ◽  
Trypan Blue Exclusion ◽  
Cellular Death ◽  
Non Thermal Plasma ◽  
Kill Curve

This research study examined the effect of non-thermal portable atmospheric air plasma system on leukemia cancer cells. Acute monocytic leukemia cells (THP-1) were exposed to atmospheric pressure non-thermal plasma. To assess death caused by plasma exposure, cells were subjected to trypan blue exclusion assays and a kill-curve and assessment of death overtime were compiled using data from the assays. In addition to this, DNA was harvested from treated and untreated samples to determine if apoptotic ladders were present. Results have indicated that non-thermal plasma can cause cell death in THP-1 cells overtime, and the death that occurs corresponds directly to the amount of time that the cells were exposed to ionized plasma. Preliminary fluorescent imaging of the treated cells revealed that higher treatment doses are not only more likely to induce cellular death but are likely to induce necrotic death, while lower treatment doses that are capable of inducing death may induce apoptotic or programmed cellular death. Ideally the results obtained from these experiments will allow for further investigation of the effects of ionized non-thermal plasma on melanoma cell lines and will lead to an inexpensive method for treating early stage skin cancer and cancerous lesions.

scholarly journals Peptone and tomato extract induced early stage of embryo development of Dendrobium phalaenopsis Orchid

2017 ◽  
Vol 1 (2) ◽  
pp. 77 ◽  
Author(s):  
Nintya Setiari ◽  
Aziz Purwantoro ◽  
Sukarti Moeljopawiro ◽  
Endang Semiarti
Keyword(s):  
Seed Germination ◽  
Embryo Development ◽  
Growth And Development ◽  
Culture Medium ◽  
Early Stage ◽  
Optimal Growth ◽  
Basic Medium ◽  
Tomato Extract ◽  
Germination And Growth

Germination and growth of orchid seeds can be accelerated by the addition of organic supplement and plant extract in culture medium. The objective of this study was to determine the effect of peptone and tomato extract on early stage of embryo development of Dendrobium phalaenopsis orchids. Orchid seeds were sown on NP and VW medium with addition of 10% of CW (NPCW and VWCW).  Five weeks after seed germination, about 58.03% seed germination was observed on VWCW medium, and only 37.45% seed germination on NPCW. Tomato extract and peptone were added in VWCW, resulting VWCWTP medium. After 4-8 weeks on VWCWTP, 94.42% seeds was germinated into plantlet, but only 67.30% germinated seeds on VWCW. To get optimal growth and development of  D.  phalaenopsis orchids embryos in the in vitro condition, supplement of 100 ml.L-1 coconut water, 100 mg.L-1 tomato extract and 2 mg.L-1 peptone into VW basic medium is required.

scholarly journals Impact of non-thermal plasma treatment on the seed germination and seedling development of carrot (Daucus carota sativus L.)

2021 ◽  
Author(s):  
Rajesh Prakash Guragain ◽  
Hom Bahadur Baniya ◽  
Suman Prakash Pradhan ◽  
Santosh Dhungana ◽  
Ganesh Kuwar Chhetri ◽  
...  
Keyword(s):  
Seed Germination ◽  
Thermal Plasma ◽  
Growth Parameters ◽  
Radical Scavenging ◽  
Seedling Development ◽  
Carotenoid Content ◽  
Favorable Effect ◽  
Seed Surface ◽  
Non Thermal Plasma ◽  
The Impact

Abstract Seed germination is a complicated physiological process that starts with the seed absorbing water and concludes with the radicle emerging. The kinetics and amount of water uptake by seeds are known to be influenced by both seed surface properties and the surrounding environment. As a result, altering seed surface features are linked to seed medium and is a valuable strategy for controlling seed germination. In the agricultural field, non-thermal plasma surface activation of seeds is currently being investigated as an efficient pre-sowing treatment for modifying seed germination. The impact of non-thermal plasma (NTP) on the germination and seedling growth of carrot seeds at room temperature and atmospheric pressure for varied treatment times was investigated in this study. Seed's germination properties and growth parameters were examined for both control and NTP treated seeds. Germination-related parameters such as germination percentage, vigor index, and chlorophyll content were all improved by NTP treatment. However, no significant changes were seen in the carotenoid content. Similarly, the in-vitro radical scavenging activities, total phenol, and total flavonoid contents in the seedlings were altered by NTP treatment. Our results indicate that NTP treatment has a favorable effect on carrots germination and seedling development.

scholarly journals Non-Thermal Plasma—A New Green Priming Agent for Plants?

2020 ◽  
Vol 21 (24) ◽  
pp. 9466
Author(s):  
Ľudmila Holubová ◽  
Stanislav Kyzek ◽  
Ivana Ďurovcová ◽  
Jana Fabová ◽  
Eva Horváthová ◽  
...  
Keyword(s):  
Thermal Plasma ◽  
Adaptive Response ◽  
Genetic Manipulation ◽  
Growth Enhancement ◽  
Mild Stress ◽  
Response Priming ◽  
Interesting Phenomenon ◽  
Surface Sterilization ◽  
Positive Effects ◽  
Non Thermal Plasma

Since the earliest agricultural attempts, humankind has been trying to improve crop quality and yields, as well as protect them from adverse conditions. Strategies to meet these goals include breeding, the use of fertilisers, and the genetic manipulation of crops, but also an interesting phenomenon called priming or adaptive response. Priming is based on an application of mild stress to prime a plant for another, mostly stronger stress. There are many priming techniques, such as osmopriming, halopriming, or using physical agents. Non-thermal plasma (NTP) represents a physical agent that contains a mixture of charged, neutral, and radical (mostly reactive oxygen and nitrogen species) particles, and can cause oxidative stress or even the death of cells or organisms upon interaction. However, under certain conditions, NTP can have the opposite effect, which has been previously documented for many plant species. Seed surface sterilization and growth enhancement are the most-reported positive effects of NTP on plants. Moreover, some studies suggest the role of NTP as a promising priming agent. This review deals with the effects of NTP treatment on plants from interaction with seed and cell surface, influence on cellular molecular processes, up to the adaptive response caused by NTP.

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