A Multispectral Camera Development: From the Prototype Assembly until Its Use in a UAV System

Multispectral imaging (MI) techniques are being used very often to identify different properties of nature in several domains, going from precision agriculture to environmental studies, not to mention quality inspection of pharmaceutical production, art restoration, biochemistry, forensic sciences or geology, just to name some. Different implementations are commercially available from the industry and yet there is quite an interest from the scientific community to spread its use to the majority of society by means of cost effectiveness and ease of use for solutions. These devices make the most sense when combined with unmanned aerial vehicles (UAVs), going a step further and alleviating repetitive routines which could be strenuous if traditional methods were adopted. In this work, a low cost and modular solution for a multispectral camera is presented, based on the use of a single panchromatic complementary metal oxide semiconductor (CMOS) sensor combined with a rotating wheel of interchangeable band pass optic filters. The system is compatible with open source hardware permitting one to capture, process, store and/or transmit data if needed. In addition, a calibration and characterization methodology has been developed for the camera, allowing not only for quantifying its performance, but also able to characterize other CMOS sensors in the market in order to select the one that best suits the budget and application. The process was experimentally validated by mounting the camera in a Dji Matrice 600 UAV to uncover vegetation indices in a reduced area of palm trees plantation. Results are presented for the normalized difference vegetation index (NDVI) showing a generated colored map with the captured information.

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Red-Green-Blue and Multispectral Imaging as Potential Tools for Estimating Growth and Nutritional Performance of Cassava under Deficit Irrigation and Potassium Fertigation

Remote Sensing ◽

10.3390/rs13040598 ◽

2021 ◽

Vol 13 (4) ◽

pp. 598

Author(s):

Daniel O. Wasonga ◽

Afrane Yaw ◽

Jouko Kleemola ◽

Laura Alakukku ◽

Pirjo S.A. Mäkelä

Keyword(s):

Water Deficit ◽

Vegetation Index ◽

Normalized Difference Vegetation Index ◽

Multispectral Imaging ◽

High Energy ◽

Plant Performance ◽

Estimation Accuracy ◽

Management Decisions ◽

Lower Estimation ◽

Low K

Cassava has high energy value and rich nutritional content, yet its productivity in the tropics is seriously constrained by abiotic stresses such as water deficit and low potassium (K) nutrition. Systems that allow evaluation of genotypes in the field and greenhouse for nondestructive estimation of plant performance would be useful means for monitoring the health of plants for crop-management decisions. We investigated whether the red–green–blue (RGB) and multispectral images could be used to detect the previsual effects of water deficit and low K in cassava, and whether the crop quality changes due to low moisture and low K could be observed from the images. Pot experiments were conducted with cassava cuttings. The experimental design was a split-plot arranged in a completely randomized design. Treatments were three irrigation doses split into various K rates. Plant images were captured beginning 30 days after planting (DAP) and ended at 90 DAP when plants were harvested. Results show that biomass, chlorophyll, and net photosynthesis were estimated with the highest accuracy (R2 = 0.90), followed by leaf area (R2 = 0.76). Starch, energy, carotenoid, and cyanide were also estimated satisfactorily (R2 > 0.80), although cyanide showed negative regression coefficients. All mineral elements showed lower estimation accuracy (R2 = 0.14–0.48) and exhibited weak associations with the spectral indices. Use of the normalized difference vegetation index (NDVI), green area (GA), and simple ratio (SR) indices allowed better estimation of growth and key nutritional traits. Irrigation dose 30% of pot capacity enriched with 0.01 mM K reduced most index values but increased the crop senescence index (CSI). Increasing K to 16 mM over the irrigation doses resulted in high index values, but low CSI. The findings indicate that RGB and multispectral imaging can provide indirect measurements of growth and key nutritional traits in cassava. Hence, they can be used as a tool in various breeding programs to facilitate cultivar evaluation and support management decisions to avert stress, such as the decision to irrigate or apply fertilizers.

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A Case Study about the Use of Precision Agriculture Technology Applied to a Zn Biofortification Workflow for Grapevine Vitis vinifera cv Moscatel

Biology and Life Sciences Forum ◽

10.3390/iecag2021-09663 ◽

2021 ◽

Vol 3 (1) ◽

pp. 2

Author(s):

Diana Daccak ◽

Inês Carmo Luís ◽

Ana Coelho Marques ◽

Ana Rita F. Coelho ◽

Cláudia Campos Pessoa ◽

...

Keyword(s):

Vitis Vinifera ◽

Precision Agriculture ◽

Vegetation Index ◽

Normalized Difference Vegetation Index ◽

Foliar Application ◽

Fold Increase ◽

Production Cycle ◽

Smart Farming ◽

Reduced Surface

As the human population is growing worldwide, the food demand is sharply increasing. Following this assumption, strategies to enhance the food production are being explored, namely, smart farming, for monitoring crops during the production cycle. In this study, a vineyard of Vitis vinifera cv. Moscatel located in Palmela (N 38°35′47.113′′ O 8°40′46.651) was submitted to a Zn biofortification workflow, through foliar application of zinc oxide (ZnO) or zinc sulfate (ZnSO4) (at a concentration of 60% and 90%—900 g·ha−1 and 1350 g·ha−1, respectively). The field morphology and vigor of the vineyard was performed through Unmanned Aerial Vehicles (UAVs) images (assessed with altimetric measurement sensors), synchronized by GPS. Drainage capacity and slopes showed one-third of the field with reduced surface drainage and a maximum variation of 0.80 m between the extremes (almost flat), respectively. The NDVI (Normalized Difference Vegetation Index) values reflected a greater vigor in treated grapes with treatment SZn90 showing a higher value. These data were interpolated with mineral content, monitored with atomic absorption analysis (showing a 1.3-fold increase for the biofortification index). It was concluded that the used technologies furnishes specific target information in real time about the crops production.

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New 30 m resolution Hong Kong climate, vegetation, and topography rasters indicate greater spatial variation than global grids within an urban mosaic

Earth System Science Data ◽

10.5194/essd-11-1083-2019 ◽

2019 ◽

Vol 11 (3) ◽

pp. 1083-1098 ◽

Cited By ~ 4

Author(s):

Brett Morgan ◽

Benoit Guénard

Keyword(s):

Hong Kong ◽

Precision Agriculture ◽

Vegetation Index ◽

Normalized Difference Vegetation Index ◽

City Planning ◽

Rapid Urbanization ◽

Local Climate ◽

Spatial Understanding ◽

Environmental And Ecological Studies ◽

Relevant Variables

Abstract. The recent proliferation of high-quality global gridded environmental datasets has spurred a renaissance of studies in many fields, including biogeography. However, these data, often 1 km at the finest scale available, are too coarse for applications such as precise designation of conservation priority areas and regional species distribution modeling, or purposes outside of biology such as city planning and precision agriculture. Further, these global datasets likely underestimate local climate variations because they do not incorporate locally relevant variables. Here we describe a comprehensive set of 30 m resolution rasters for Hong Kong, a small tropical territory with highly variable terrain where intense anthropogenic disturbance meets a robust protected area system. The data include topographic variables, a Normalized Difference Vegetation Index raster, and interpolated climate variables based on weather station observations. We present validation statistics that convey each climate variable's reliability and compare our results to a widely used global dataset, finding that our models consistently reflect greater climatic variation. To our knowledge, this is the first set of published environmental rasters specific to Hong Kong. We hope this diverse suite of geographic data will facilitate future environmental and ecological studies in this region of the world, where a spatial understanding of rapid urbanization, introduced species pressure, and conservation efforts is critical. The dataset (Morgan and Guénard, 2018) is accessible at https://doi.org/10.6084/m9.figshare.6791276.

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Application of Phenotyping Methods in Detection of Drought and Salinity Stress in Basil (Ocimum basilicum L.)

Frontiers in Plant Science ◽

10.3389/fpls.2021.629441 ◽

2021 ◽

Vol 12 ◽

Author(s):

Boris Lazarević ◽

Zlatko Šatović ◽

Ana Nimac ◽

Monika Vidak ◽

Jerko Gunjača ◽

...

Keyword(s):

Drought Stress ◽

Salinity Stress ◽

Vegetation Index ◽

Normalized Difference Vegetation Index ◽

Multispectral Imaging ◽

Ocimum Basilicum ◽

Phenotypic Traits ◽

Severe Drought ◽

Moderate Salinity ◽

Automated Phenotyping

Basil is one of the most widespread aromatic and medicinal plants, which is often grown in drought- and salinity-prone regions. Often co-occurrence of drought and salinity stresses in agroecosystems and similarities of symptoms which they cause on plants complicates the differentiation among them. Development of automated phenotyping techniques with integrative and simultaneous quantification of multiple morphological and physiological traits enables early detection and quantification of different stresses on a whole plant basis. In this study, we have used different phenotyping techniques including chlorophyll fluorescence imaging, multispectral imaging, and 3D multispectral scanning, aiming to quantify changes in basil phenotypic traits under early and prolonged drought and salinity stress and to determine traits which could differentiate among drought and salinity stressed basil plants. Ocimum basilicum “Genovese” was grown in a growth chamber under well-watered control [45–50% volumetric water content (VWC)], moderate salinity stress (100 mM NaCl), severe salinity stress (200 mM NaCl), moderate drought stress (25–30% VWC), and severe drought stress (15–20% VWC). Phenotypic traits were measured for 3 weeks in 7-day intervals. Automated phenotyping techniques were able to detect basil responses to early and prolonged salinity and drought stress. In addition, several phenotypic traits were able to differentiate among salinity and drought. At early stages, low anthocyanin index (ARI), chlorophyll index (CHI), and hue (HUE2D), and higher reflectance in red (RRed), reflectance in green (RGreen), and leaf inclination (LINC) indicated drought stress. At later stress stages, maximum fluorescence (Fm), HUE2D, normalized difference vegetation index (NDVI), and LINC contribute the most to the differentiation among drought and non-stressed as well as among drought and salinity stressed plants. ARI and electron transport rate (ETR) were best for differentiation of salinity stressed plants from non-stressed plants both at early and prolonged stress.

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Inherent Reflectance Variability of Vegetation

Agriculture ◽

10.3390/agriculture9110246 ◽

2019 ◽

Vol 9 (11) ◽

pp. 246 ◽

Cited By ~ 1

Author(s):

Baabak Mamaghani ◽

M. Grady Saunders ◽

Carl Salvaggio

Keyword(s):

Remote Sensing ◽

Precision Agriculture ◽

Vegetation Index ◽

Normalized Difference Vegetation Index ◽

Remote Sensing Data ◽

Time Of Day ◽

Unmanned Aircraft ◽

P Value ◽

Ground Sample ◽

Significant Difference

With the inception of small unmanned aircraft systems (sUAS), remotely sensed images have been captured much closer to the ground, which has meant better resolution and smaller ground sample distances (GSDs). This has provided the precision agriculture community with the ability to analyze individual plants, and in certain cases, individual leaves on those plants. This has also allowed for a dramatic increase in data acquisition for agricultural analysis. Because satellite and manned aircraft remote sensing data collections had larger GSDs, self-shadowing was not seen as an issue for agricultural remote sensing. However, sUAS are able to image these shadows which can cause issues in data analysis. This paper investigates the inherent reflectance variability of vegetation by analyzing six Coneflower plants, as a surrogate for other cash crops, across different variables. These plants were measured under different forecasts (cloudy and sunny), at different times (08:00 a.m., 09:00 a.m., 10:00 a.m., 11:00 a.m. and 12:00 p.m.), and at different GSDs (2, 4 and 8 cm) using a field portable spectroradiometer (ASD Field Spec). In addition, a leafclip spectrometer was utilized to measure individual leaves on each plant in a controlled lab environment. These spectra were analyzed to determine if there was any significant difference in the health of the various plants measured. Finally, a MicaSense RedEdge-3 multispectral camera was utilized to capture images of the plants every hour to analyze the variability produced by a sensor designed for agricultural remote sensing. The RedEdge-3 was held stationary at 1.5 m above the plants while collecting all images, which produced a GSD of 0.1 cm/pixel. To produce 2, 4, and 8 cm GSD, the MicaSense RedEdge-3 would need to be at an altitude of 30.5 m, 61 m and 122 m respectively. This study did not take background effects into consideration for either the ASD or MicaSense. Results showed that GSD produced a statistically significant difference (p < 0.001) in Normalized Difference Vegetation Index (NDVI, a commonly used metric to determine vegetation health), R 2 values demonstrated a low correlation between time of day and NDVI, and a one-way ANOVA test showed no statistically significant difference in the NDVI computed from the leafclip probe (p-value of 0.018). Ultimately, it was determined that the best condition for measuring vegetation reflectance was on cloudy days near noon. Sunny days produced self-shadowing on the plants which increased the variability of the measured reflectance values (higher standard deviations in all five RedEdge-3 channels), and the shadowing of the plants decreased as time approached noon. This high reflectance variability in the coneflower plants made it difficult to accurately measure the NDVI.

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Spectral signature of brown rust and orange rust in sugarcane

Revista Facultad de Ingeniería Universidad de Antioquia ◽

10.17533/udea.redin.20191042 ◽

2020 ◽

pp. 9-20

Author(s):

Eniel Rodríguez-Machado ◽

Osmany Aday-Díaz ◽

Luis Hernández-Santana ◽

Jorge Luís Soca-Muñoz ◽

Rubén Orozco-Morales

Keyword(s):

Precision Agriculture ◽

Spectral Reflectance ◽

Vegetation Index ◽

Near Infrared ◽

Normalized Difference Vegetation Index ◽

Brown Rust ◽

Spectral Signature ◽

Spatial And Temporal Variability ◽

Mean Values ◽

Index Value

Precision agriculture, making use of the spatial and temporal variability of cultivable land, allows farmers to refine fertilization, control field irrigation, estimate planting productivity, and detect pests and disease in crops. To that end, this paper identifies the spectral reflectance signature of brown rust (Puccinia melanocephala) and orange rust (Puccinia kuehnii), which contaminate sugar cane leaves (Saccharum spp.). By means of spectrometry, the mean values and standard deviations of the spectral reflectance signature are obtained for five levels of contamination of the leaves in each type of rust, observing the greatest differences between healthy and diseased leaves in the red (R) and near infrared (NIR) bands. With the results obtained, a multispectral camera was used to obtain images of the leaves and calculate the Normalized Difference Vegetation Index (NDVI). The results identified the presence of both plagues by differentiating healthy from contaminated leaves through the index value with an average difference of 11.9% for brown rust and 9.9% for orange rust.

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Proposal for an Embedded System Architecture Using a GNDVI Algorithm to Support UAV-Based Agrochemical Spraying

Sensors ◽

10.3390/s19245397 ◽

2019 ◽

Vol 19 (24) ◽

pp. 5397 ◽

Cited By ~ 1

Author(s):

Maik Basso ◽

Diego Stocchero ◽

Renato Ventura Bayan Henriques ◽

André Luis Vian ◽

Christian Bredemeier ◽

...

Keyword(s):

Control System ◽

Embedded System ◽

Precision Agriculture ◽

System Architecture ◽

Vegetation Index ◽

Normalized Difference Vegetation Index ◽

Raspberry Pi ◽

Research Station ◽

High Definition ◽

Cpu Usage

An important area in precision agriculture is related to the efficient use of chemicals applied onto fields. Efforts have been made to diminish their use, aiming at cost reduction and fewer chemical residues in the final agricultural products. The use of unmanned aerial vehicles (UAVs) presents itself as an attractive and cheap alternative for spraying pesticides and fertilizers compared to conventional mass spraying performed by ordinary manned aircraft. Besides being cheaper than manned aircraft, small UAVs are capable of performing fine-grained instead of the mass spraying. Observing this improved method, this paper reports the design of an embedded real-time UAV spraying control system supported by onboard image processing. The proposal uses a normalized difference vegetation index (NDVI) algorithm to detect the exact locations in which the chemicals are needed. Using this information, the automated spraying control system performs punctual applications while the UAV navigates over the crops. The system architecture is designed to run on low-cost hardware, which demands an efficient NDVI algorithm. The experiments were conducted using Raspberry Pi 3 as the embedded hardware. First, experiments in a laboratory were conducted in which the algorithm was proved to be correct and efficient. Then, field tests in real conditions were conducted for validation purposes. These validation tests were performed in an agronomic research station with the Raspberry hardware integrated into a UAV flying over a field of crops. The average CPU usage was about 20% while memory consumption was about 70 MB for high definition images, with 4% CPU usage and 20.3 MB RAM being observed for low-resolution images. The average current measured to execute the proposed algorithm was 0.11 A. The obtained results prove that the proposed solution is efficient in terms of processing and energy consumption when used in embedded hardware and provides measurements which are coherent with the commercial GreenSeeker equipment.

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Sustainable Agriculture by Increasing Nitrogen Fertilizer Efficiency Using Low-Resolution Camera Mounted on Unmanned Aerial Vehicles

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16203893 ◽

2019 ◽

Vol 16 (20) ◽

pp. 3893 ◽

Cited By ~ 1

Author(s):

Kim ◽

Min ◽

Kim ◽

Silva ◽

Hyun ◽

...

Keyword(s):

Chlorophyll Content ◽

Nitrogen Fertilizer ◽

Precision Agriculture ◽

Vegetation Index ◽

Normalized Difference Vegetation Index ◽

Nitrogen Deficiency ◽

Underground Water ◽

Fertilizer Efficiency ◽

Low Resolution ◽

Effective Manner

Nitrogen use efficiency in modern agriculture is very low. It means that a lot of synthetic chemicals are wasted rather than utilized by crops. This can cause more problems where the soil surface is thin and rocky like Jeju Island in the Republic of Korea. This is because overly used nitrogen fertilizer can be washed into the underground water and pollute it. Thus, it would be important to monitor the nitrogen deficiency of crops in the field to provide the right amount of nitrogen in a timely manner so that nitrogen waste can be limited. To achieve this, the normalized difference vegetation index (NDVI) was used to monitor chlorophyll content, which is tightly associated with nitrogen content in the buckwheat field. The NDVI was calculated with the data obtained by a low-resolution camera mounted on an unmanned aerial vehicle. The results showed that the NDVI can estimate the chlorophyll content of buckwheat. These simple but clear results imply that precision agriculture could be achieved even with a low-resolution camera in a cost-effective manner to reduce the pollution of underground water.

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Assessment of Maize Growth and Development with High- and Medium-Resolution Remote Sensing Products

Agronomy ◽

10.3390/agronomy11050940 ◽

2021 ◽

Vol 11 (5) ◽

pp. 940

Author(s):

Rocío Ballesteros ◽

Miguel A. Moreno ◽

Fellype Barroso ◽

Laura González-Gómez ◽

José F. Ortega

Keyword(s):

Remote Sensing ◽

Precision Agriculture ◽

Vegetation Index ◽

Normalized Difference Vegetation Index ◽

Landsat Imagery ◽

Canopy Cover ◽

Remote Sensing Data ◽

Multilinear Regression ◽

Area Index ◽

Canopy Development

The availability of a great amount of remote sensing data for precision agriculture purposes has set the question of which resolution and indices, derived from satellites or unmanned aerial vehicles (UAVs), offer the most accurate results to characterize vegetation. This study focused on assessing, comparing, and discussing the performances and limitations of satellite and UAV-based imagery in terms of canopy development, i.e., the leaf area index (LAI), and yield, i.e., the dry aboveground biomass (DAGB), for maize. Three commercial maize fields were studied over four seasons to obtain the LAI and DAGB. The normalized difference vegetation index (NDVI) and visible atmospherically resistant index (VARI) from satellite platforms (Landsat 5TM, 7 ETM+, 8OLI, and Sentinel 2A MSI) and the VARI and green canopy cover (GCC) from UAV imagery were compared. The remote sensing predictors in addition to the growing degree days (GDD) were assessed to estimate the LAI and DAGB using multilinear regression models (MRMs). For LAI estimation, better adjustments were obtained when predictors from the UAV platform were considered. The DAGB estimation revealed similar adjustments for both platforms, although the Landsat imagery offered slightly better adjustments. The results obtained in this study demonstrate the advantage of remote sensing platforms as a useful tool to estimate essential agronomic features.

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Monitoring of a Calcium Biofortification Workflow for Tubers of Solanum tuberosum L. cv. Picasso Using Smart Farming Technology

Biology and Life Sciences Forum ◽

10.3390/iecag2021-09660 ◽

2021 ◽

Vol 3 (1) ◽

pp. 18

Author(s):

Ana Rita F. Coelho ◽

Inês Carmo Luís ◽

Ana Coelho Marques ◽

Cláudia Campos Pessoa ◽

Diana Daccak ◽

...

Keyword(s):

Solanum Tuberosum ◽

Precision Agriculture ◽

Vegetation Index ◽

Normalized Difference Vegetation Index ◽

Foliar Application ◽

Solanum Tuberosum L ◽

Test System ◽

Experimental Production ◽

Large Crop ◽

The Impact

Due to the rapid growth of the population worldwide and the need to provide food safety in large crop productions, UAVs (unmanned aerial vehicles) are being used in agriculture to provide valuable data for decision making. Accordingly, through precision agriculture, efficient management of resources, using data obtained by the technologies, is possible. Through remote sensed data collected in a crop region, it is possible to create NDVI (normalized difference vegetation index) maps, which are a powerful tool to detect stresses, namely, in plants. Accordingly, using smart farm technology, this study aimed to assess the impact of Ca biofortification on leaves of Solanum tuberosum L. cv. Picasso. As such, using an experimental production field of potato tubers (GPS coordinates: 39°16′38,816′′ N; 9°15′9128′′ W) as a test system, plants were submitted to a Ca biofortification workflow through foliar spraying with CaCl2 or, alternatively, chelated calcium (Ca-EDTA) at concentrations of 12 and 24 kg·ha−1. A lower average NDVI in Ca-EDTA 12 kg·ha−1 treatment after the fourth foliar application was found, which, through the application of the CieLab scale, correlated with lower L (darker color) and hue parameters, regarding control plants. Additionally, a higher Ca content was quantified in the leaves. The obtained data are discussed, and it is concluded that Ca-EDTA 12 kg·ha−1 triggers lower vigor in Picasso potatoes leaves.

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